FastQCFastQC Report
Wed 8 May 2024
EGAF00002877634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213900
Total Bases15.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19740.9228611500701263No Hit
TCCCTACACGACGCGGTTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG12950.6054230949041608Illumina Single End PCR Primer 1 (100% over 30bp)
TTCCCTACACGACGCGGTTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGA8110.3791491351098644Illumina Single End PCR Primer 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6190.28938756428237494No Hit
CTACACGACGCGGTTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT4040.18887330528284244Illumina Single End PCR Primer 1 (100% over 33bp)
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA3860.1804581580177653No Hit
ACACGACGCGGTTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG3010.1407199625993455Illumina Single End PCR Primer 1 (100% over 35bp)
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG2830.13230481533426836No Hit
CCCTACACGACGCGGTTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT2410.11266947171575503Illumina Single End PCR Primer 1 (100% over 31bp)
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGT2220.10378681626928472No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA2150.10051425899953248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)