FastQCFastQC Report
Tue 7 May 2024
EGAF00002877637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10995
Total Bases780.6 kbp
Sequences flagged as poor quality0
Sequence length71
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7757.048658481127785No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA1541.400636653024102No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG760.6912232833105958No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA690.6275579809004093No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC510.4638472032742156No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT410.37289677125966353No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC400.36380172805820826No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.3274215552523874No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG330.30013642564802184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310.2819463392451114No Hit
GTTTATTACTGAAGATTGATCCATTGTTGCCTAAAGAAGTAAAAAATGAA300.2728512960436562No Hit
AACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT260.23647112323783537No Hit
GATCACAGTAGGGTGAAGTTAAGACCTTTACCAGGAAAAGACTCTAAGCA260.23647112323783537No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG260.23647112323783537No Hit
ATGCCTAAATGTGGTGAATTGTGAAATTACTTTCAGTGGTTTAAGAAGCA250.22737608003638018No Hit
GATCCATTGTTGCCTAAAGAAGTAAAAAATGAAGAAAGTGAAAAAGAAGA250.22737608003638018No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC240.21828103683492497No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA240.21828103683492497No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAA220.20009095043201455No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATA220.20009095043201455No Hit
TGATAATACAGCATAATTTTTACATTTGATGTCTTGTTATTGATCATACT220.20009095043201455No Hit
CAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGA210.19099590723055934No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGT210.19099590723055934No Hit
GTGCTAGCTCCTCCCACCTCAGCACCTGCTGCATCTGGAGCAGCCCAAGC210.19099590723055934No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA200.18190086402910413No Hit
CCTGCAAGATGGACACGAGCCACAAGCTGCACTGTGAACCTGGGCACTCC200.18190086402910413No Hit
TAATTAAGCTCTAGGTCAGATAACAATGGAGTAAAGTGATACCTGCATAA200.18190086402910413No Hit
CTATAGCGATGCTATGTTGAATGTATTCTGATCGGATCGTTTATGGTTAT200.18190086402910413No Hit
CCTTCTTTGATAATGTTAAATTTGTAAGTTTCAGGTGACATGTGAAACCT200.18190086402910413No Hit
GTGTGGATTTCCACTCTTTTCAGTCCTTCATGTTAAAGATTTAGACACCA190.17280582082764895No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA190.17280582082764895No Hit
CTATAATCTCCAACTCCTGAAAACCCCTCTCTCAACTAATACTTTGCTGT190.17280582082764895No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG190.17280582082764895No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG190.17280582082764895No Hit
CCCCATCACGGTTAACGAGCATCTTTGGTCTCTTGTGGAATTTGAACTAA180.1637107776261937No Hit
GGAGTTTTGGTTTTAATTAGATGGTTCACTACCACTGGGTAGTGCCATTT180.1637107776261937No Hit
CCCAGGTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACTCGGGAGCC180.1637107776261937No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1637107776261937No Hit
GTGGGATCGTTTCCAGATGAGAATTCACAAGCGACTCATTGACTTGCACA170.15461573442473853No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAA170.15461573442473853No Hit
ACCGTACCCGCCAAGCTGTCATCACAAAAGAGTTGATTGAAATTATCTCT170.15461573442473853No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTC170.15461573442473853No Hit
CATCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGAAAATA170.15461573442473853No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15461573442473853No Hit
AGGTGGAACCGTCCCAAAATGTCCCTTGCTCAGAAGAAGGATCGGGTAGC160.1455206912232833No Hit
TTTCAGTGGTTTAAGAAGCAATGGAGAAAATGATAATACAGCATAATTTT160.1455206912232833No Hit
AGATTGATCCATTGTTGCCTAAAGAAGTAAAAAATGAAGAAAGTGAAAAA160.1455206912232833No Hit
ATCAGGTGGAGAAGCACTTCAACGCTGGACGAAGTAAAGATTATTGTTGT160.1455206912232833No Hit
ACATGGAGAAACCCCATCTCTACTCAAAATACAAAGTTAGCTGGATGTGG160.1455206912232833No Hit
CCTTATCGAGGAGCAGAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCAC160.1455206912232833No Hit
GACCAATATGGTGAAACCCCGTCTCTGCTAAAAATACAAAAAAATTAGCT160.1455206912232833No Hit
CATTAGTGCAACTACTACCTTGGTGCCTCAGTTTACCCATCTGTAAAATG160.1455206912232833No Hit
ATATTGTGATTTGGCCTATATACTGATCCAAAGAAAAGTCTCGTGAGTGT160.1455206912232833No Hit
CCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGT160.1455206912232833No Hit
TGTCAGCAGGGCCTATGGTGGTTCCATGTGTGCTAAATGTGTTCGTGACA160.1455206912232833No Hit
GATCCGAGTCGTCCGGAAATCCATTGCCCGTGTTCTCACAGTTATTAACC160.1455206912232833No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT160.1455206912232833No Hit
CAGATAAAACAAAATCAACAAAAATTAAGTTCATTTTGTGATTAAACCTG150.1364256480218281No Hit
TTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAG150.1364256480218281No Hit
ATTTATTCCCTTCTATTCAAGGCCAGTTAGGAATGCAATTATTTTTTTTT150.1364256480218281No Hit
GAATTCTGCTTTCCTATAATTTCTACCTATTGGGCCTTGTTCTGTTCTCT150.1364256480218281No Hit
GCGTCTTGTTACTCCACGTGTCCTGCAGCACAAACGGCGGCGTATTGCTC150.1364256480218281No Hit
GTCCAGGTGGAGGAGGTGCTGGCGAGTTTGAAATTTGGGAGTCTTGACCG150.1364256480218281No Hit
GTTGCCCGGTTGGGAATGTGCTTGGCGCTGACCCTGCGGGCATCTGACTG150.1364256480218281No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT150.1364256480218281No Hit
GCAATGGAGAAAATGATAATACAGCATAATTTTTACATTTGATGTCTTGT150.1364256480218281No Hit
GGTTTATTACTGAAGATTGATCCATTGTTGCCTAAAGAAGTAAAAAATGA150.1364256480218281No Hit
ACCAACAGGAAGAAACCCCACCTTTACTAAAAATACAAAATTAGCAGGGT150.1364256480218281No Hit
CACTCCTAGAGAAAGGTACACAGTTGCCCGGTTGGGAATGTGCTTGGCGC150.1364256480218281No Hit
CCAACATGCTGCTGATCGGGGTCCATGGGCCCACCACCCCCTGCGAGGAG150.1364256480218281No Hit
GTGAGTTTCCTTTTCTCCATTTCTAGCATATGGACACCTGGCCTCTGCTT140.1273306048203729No Hit
GAATAGTTCCACATACTTTCAGAAGAGCTCAGCAGGGCCCTGCCTGGTCT140.1273306048203729No Hit
AAAGAAGATGCCTAAATGTGGTGAATTGTGAAATTACTTTCAGTGGTTTA140.1273306048203729No Hit
CAACTGTAAATAATGTACATTTAATGTCACAAGAAAAAAATGTCTTCTGC140.1273306048203729No Hit
GGTCATCACTGACAAAGAGAAGGCAGAGAAACTGAAGCAGTCCCTGCCCC140.1273306048203729No Hit
TGTCTGTACACCTGTCCCCCTGCTTCAGGGACGTCCAGATCGGTGACATC140.1273306048203729No Hit
CTTCTCATCTGCACATGGCACATATTCCAAAATTGACCCAAAAATTGAGC140.1273306048203729No Hit
CTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1273306048203729No Hit
ATGTTGAAGTTGCATTGTAGAAGTTTAGAAGCTCTGGCTATGGGTTGCCT140.1273306048203729No Hit
CTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCT140.1273306048203729No Hit
CCTCTAGTCCTAGCTACTCGGGAGGCTGAGGCACGAGAATCACTTGAACC130.11823556161891768No Hit
GTACTGAGTATCTCAATGATTTCTTCATCCAACAAGTAGTTATTGTGAGT130.11823556161891768No Hit
CACTAGAGGAAATGCCAATAAAACACAGAAAGATACTGGAGTAAATGAAA130.11823556161891768No Hit
CCTAAGGAAAAGGCCCAACCCCTGGAAGACCTGGCCGGCTTCACAGAGCT130.11823556161891768No Hit
AGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTAT130.11823556161891768No Hit
TATTCTAACAGCAGGAATTTCAAGCCAGGTAAGGGGAGCTATGTCAGGCT130.11823556161891768No Hit
CCATTAAAGAGATCCTGGGGACTGCCCAGTCAGTGGGCTGTAATGTTGAT130.11823556161891768No Hit
GAATAACACTGCTGCAGATGACAAGCAGCCTTATGAAAAGAAGGCTGCGA130.11823556161891768No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT130.11823556161891768No Hit
GATAATAAAGAATGTAATTAAATTTAAATTTCTTGGAATTCCCAGTTGTA130.11823556161891768No Hit
TCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAAC130.11823556161891768No Hit
GCCTAAATGTGGTGAATTGTGAAATTACTTTCAGTGGTTTAAGAAGCAAT130.11823556161891768No Hit
GCCCAGTCAGTGGGCTGTAATGTTGATGGCCGCCATCCTCATGACATCAT130.11823556161891768No Hit
TTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCC120.10914051841746249No Hit
GTATTAGACTATCTCTCCGACTTCATCTGCTGTTTATATGGTGTACCAAG120.10914051841746249No Hit
AATAAAATCATAGAACTGATTAGGAGGCCCTCTTAAAGAGGTCAACTCAT120.10914051841746249No Hit
GAAATGACCACGTGAAATTTGCCTCTGTCCAAACATTTCATCCGTGTGTA120.10914051841746249No Hit
CTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAAAAAAAAAA120.10914051841746249No Hit
AACCAGACTCAGAAAGAAAACCTCAGGAAATTCTACAAGGGCAAGAAGTA120.10914051841746249No Hit
CCTCAGTGTATAAATTTATCCTCACACCTGAAATTCTTTTTCTGAACTAA120.10914051841746249No Hit
GGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGA120.10914051841746249No Hit
CATCAAAGCCCTCAAGGAACCACCAAGAGACAGAAAGAAACAGAAAAACA120.10914051841746249No Hit
ACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAA120.10914051841746249No Hit
TATTATCTTTAAAAAGTTAAATGTTTTTTTAATCTTCAAGAAATCATGCT120.10914051841746249No Hit
CTCCAGCTTGTATTTAAGTCCCTGGGCTGCCCCCTTGGGGTGCCCCCCGC120.10914051841746249No Hit
CCTGGGTAGGGCGAAAGTTAATCTAGCCATGTGATTTTTCAGAAAAGTAA120.10914051841746249No Hit
TTGTTATAATGAATGAAACATTTTGTCATATAAGATTCATATTTACTTCT110.10004547521600728No Hit
CAAACCATTTTATTTTTTGGGCCATTACCCCATACCCCTTATTGCTGCCA110.10004547521600728No Hit
GTGAATACATGGATAAAAATAATGTGAGGCGACAAGCAACAACAATCATA110.10004547521600728No Hit
GATCCAGAAGGAGCTCACCATTGGCTCGAAGCTGCAGGATGCTGAAATTG110.10004547521600728No Hit
ATAGTGACGAATGTCTGGCAGTGAGGACACATTTTGGCATTCTTGCTGAC110.10004547521600728No Hit
GCTAAAGACTTCCCTGTCTGCTCACATCTGGTCTGTTCCCCAGTATCCTC110.10004547521600728No Hit
GCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACA110.10004547521600728No Hit
GGAGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT110.10004547521600728No Hit
AGCCAGCTGAGGGGAAGTTGCTGGTGAGTTTCCTTTTCTCCATTTCTAGC110.10004547521600728No Hit
GGAATAAGGAATGTGCCATACCGAATCCGTGTGCGGCTGTCCAGAAAACG110.10004547521600728No Hit
GATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCC110.10004547521600728No Hit
ATCCAGCTGCCTATGAAAATGACAAATGAGCAACGCATCCGGATGACGGT110.10004547521600728No Hit
GACTATCTGTTCATGAGAGAAATTTTCTGTAAGCTTGCTGTTTTACAGGG110.10004547521600728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)