FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10995
Sequences flagged as poor quality0
Sequence length71
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7516.830377444292861No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1030.9367894497498863No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT700.6366530241018644No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA650.5911778080945885No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC460.41837198726693947No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT400.36380172805820826No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.3274215552523874No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC340.30923146884947705No Hit
GTTTATTACTGAAGATTGATCCATTGTTGCCTAAAGAAGTAAAAAATGAAGAAAGTGAAAAAGAAGATGCC280.2546612096407458No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250.22737608003638018No Hit
GATCACAGTAGGGTGAAGTTAAGACCTTTACCAGGAAAAGACTCTAAGCACAGCGACTACATTAATGCAAA240.21828103683492497No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC240.21828103683492497No Hit
GATCCATTGTTGCCTAAAGAAGTAAAAAATGAAGAAAGTGAAAAAGAAGATGCCTAAATGTGGTGAATTGT230.20918599363346974No Hit
ATGCCTAAATGTGGTGAATTGTGAAATTACTTTCAGTGGTTTAAGAAGCAATGGAGAAAATGATAATACAG220.20009095043201455No Hit
TGATAATACAGCATAATTTTTACATTTGATGTCTTGTTATTGATCATACTTGAAAGTGAACTTTAACATTG220.20009095043201455No Hit
AACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC210.19099590723055934No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCA210.19099590723055934No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA210.19099590723055934No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.19099590723055934No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGG200.18190086402910413No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC190.17280582082764895No Hit
CAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGA190.17280582082764895No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.17280582082764895No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1637107776261937No Hit
CTATAATCTCCAACTCCTGAAAACCCCTCTCTCAACTAATACTTTGCTGTTGAAATGTTGTGAAATGTTAA180.1637107776261937No Hit
CCCCATCACGGTTAACGAGCATCTTTGGTCTCTTGTGGAATTTGAACTAAAACTATGAGCCTTATTCAATA180.1637107776261937No Hit
CTATAGCGATGCTATGTTGAATGTATTCTGATCGGATCGTTTATGGTTATTTACAATGCAGTTACTCTTGA180.1637107776261937No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG180.1637107776261937No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC170.15461573442473853No Hit
GGAGTTTTGGTTTTAATTAGATGGTTCACTACCACTGGGTAGTGCCATTTTGGCCGGACATGGTTGGGGTA170.15461573442473853No Hit
CCTTCTTTGATAATGTTAAATTTGTAAGTTTCAGGTGACATGTGAAACCTTTTTTAAGATTTTTCTCAAAG170.15461573442473853No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15461573442473853No Hit
GTGTGGATTTCCACTCTTTTCAGTCCTTCATGTTAAAGATTTAGACACCACATACAACTGGTAAAGGACGT170.15461573442473853No Hit
CATCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGAAAATACTACAGAGTGTGCACACTGGC160.1455206912232833No Hit
TTTCAGTGGTTTAAGAAGCAATGGAGAAAATGATAATACAGCATAATTTTTACATTTGATGTCTTGTTATT160.1455206912232833No Hit
GTGGGATCGTTTCCAGATGAGAATTCACAAGCGACTCATTGACTTGCACAGTCCTTCTGAGATTGTTAAGC160.1455206912232833No Hit
ACCGTACCCGCCAAGCTGTCATCACAAAAGAGTTGATTGAAATTATCTCTGGTGCTGCAGCTCTGGATTAA160.1455206912232833No Hit
CCTTATCGAGGAGCAGAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGCTAAATAAA160.1455206912232833No Hit
TGTCAGCAGGGCCTATGGTGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTA150.1364256480218281No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGT150.1364256480218281No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1364256480218281No Hit
ACCAACAGGAAGAAACCCCACCTTTACTAAAAATACAAAATTAGCAGGGTATAGTGGCACATGCCTGTAAT150.1364256480218281No Hit
CCCAGGTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACTCGGGAGCCTGAGGCAGAAGAATCACTTGA150.1364256480218281No Hit
CAGATAAAACAAAATCAACAAAAATTAAGTTCATTTTGTGATTAAACCTGCAACCATTTTTCCATTACTTT150.1364256480218281No Hit
GTTGCCCGGTTGGGAATGTGCTTGGCGCTGACCCTGCGGGCATCTGACTGGTCTTCCAGCTCAGGAAAAAG150.1364256480218281No Hit
CATTAGTGCAACTACTACCTTGGTGCCTCAGTTTACCCATCTGTAAAATGGGTAAGCATAGCCACTGGTGG150.1364256480218281No Hit
AGGTGGAACCGTCCCAAAATGTCCCTTGCTCAGAAGAAGGATCGGGTAGCTCAAAAGAAGGCAAGCTTCCT140.1273306048203729No Hit
GGTCATCACTGACAAAGAGAAGGCAGAGAAACTGAAGCAGTCCCTGCCCCCCGGTTTGGCAGTGAAGGAAC140.1273306048203729No Hit
GCGTCTTGTTACTCCACGTGTCCTGCAGCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAGA140.1273306048203729No Hit
TAATTAAGCTCTAGGTCAGATAACAATGGAGTAAAGTGATACCTGCATAACTTTACTCCATTGTTATCTGA140.1273306048203729No Hit
GTCCAGGTGGAGGAGGTGCTGGCGAGTTTGAAATTTGGGAGTCTTGACCGGGCGCAGTGGCTCACGCCTGT140.1273306048203729No Hit
AAAGAAGATGCCTAAATGTGGTGAATTGTGAAATTACTTTCAGTGGTTTAAGAAGCAATGGAGAAAATGAT140.1273306048203729No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA140.1273306048203729No Hit
GTGCTAGCTCCTCCCACCTCAGCACCTGCTGCATCTGGAGCAGCCCAAGCCTCAGGATGGACAAGAGGAAA140.1273306048203729No Hit
GAATTCTGCTTTCCTATAATTTCTACCTATTGGGCCTTGTTCTGTTCTCTGGAACTAAACAGAACAACCAT140.1273306048203729No Hit
ATGTTGAAGTTGCATTGTAGAAGTTTAGAAGCTCTGGCTATGGGTTGCCTAAATTGATGTTTTGAGGAAGC140.1273306048203729No Hit
GAATAGTTCCACATACTTTCAGAAGAGCTCAGCAGGGCCCTGCCTGGTCTCCCGAGCATCATGAGTAACGT140.1273306048203729No Hit
ATATTGTGATTTGGCCTATATACTGATCCAAAGAAAAGTCTCGTGAGTGTATTAATTTTGGGTGTCTTTAG140.1273306048203729No Hit
GCAATGGAGAAAATGATAATACAGCATAATTTTTACATTTGATGTCTTGTTATTGATCATACTTGAAAGTG140.1273306048203729No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1273306048203729No Hit
CAACTGTAAATAATGTACATTTAATGTCACAAGAAAAAAATGTCTTCTGCAAATTTTCTAGTATAACAGAA140.1273306048203729No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT140.1273306048203729No Hit
GGTTTATTACTGAAGATTGATCCATTGTTGCCTAAAGAAGTAAAAAATGAAGAAAGTGAAAAAGAAGATGC130.11823556161891768No Hit
ACATGGAGAAACCCCATCTCTACTCAAAATACAAAGTTAGCTGGATGTGGTGGCACATGCCGGTAATCCCA130.11823556161891768No Hit
AGATTGATCCATTGTTGCCTAAAGAAGTAAAAAATGAAGAAAGTGAAAAAGAAGATGCCTAAATGTGGTGA130.11823556161891768No Hit
GCCTAAATGTGGTGAATTGTGAAATTACTTTCAGTGGTTTAAGAAGCAATGGAGAAAATGATAATACAGCA130.11823556161891768No Hit
GCCCAGTCAGTGGGCTGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACATCAACAGTGGTGC130.11823556161891768No Hit
CCAACATGCTGCTGATCGGGGTCCATGGGCCCACCACCCCCTGCGAGGAGGTCTCCATGAAGCATGTAGGC130.11823556161891768No Hit
GATAATAAAGAATGTAATTAAATTTAAATTTCTTGGAATTCCCAGTTGTATATTCATCACCTTTGTAGCAT130.11823556161891768No Hit
CACTAGAGGAAATGCCAATAAAACACAGAAAGATACTGGAGTAAATGAAAAGGCAAGAGAGAAATTTTATG130.11823556161891768No Hit
CTTCTCATCTGCACATGGCACATATTCCAAAATTGACCCAAAAATTGAGCATAAAGCAATCATCAGCAAAT130.11823556161891768No Hit
CCTCTAGTCCTAGCTACTCGGGAGGCTGAGGCACGAGAATCACTTGAACCTGGGAGGCGGAGGTTGCAGTG130.11823556161891768No Hit
GAATAACACTGCTGCAGATGACAAGCAGCCTTATGAAAAGAAGGCTGCGAAGCTGAAGGAAAAATACGAAA130.11823556161891768No Hit
CCTGGGTAGGGCGAAAGTTAATCTAGCCATGTGATTTTTCAGAAAAGTAAGTGGAACATGCTGCCACTTTT120.10914051841746249No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA120.10914051841746249No Hit
TATTCTAACAGCAGGAATTTCAAGCCAGGTAAGGGGAGCTATGTCAGGCTGCCAGATGGTGGGGAAGACAG120.10914051841746249No Hit
GTATTAGACTATCTCTCCGACTTCATCTGCTGTTTATATGGTGTACCAAGCGCCTATGGAGTTGTCACTAT120.10914051841746249No Hit
GATCCGAGTCGTCCGGAAATCCATTGCCCGTGTTCTCACAGTTATTAACCAGACTCAGAAAGAAAACCTCA120.10914051841746249No Hit
CTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10914051841746249No Hit
TGTCTGTACACCTGTCCCCCTGCTTCAGGGACGTCCAGATCGGTGACATCGTCACAGTGGGCGAGTGCCGG120.10914051841746249No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT120.10914051841746249No Hit
CACTCCTAGAGAAAGGTACACAGTTGCCCGGTTGGGAATGTGCTTGGCGCTGACCCTGCGGGCATCTGACT120.10914051841746249No Hit
GTGAGTTTCCTTTTCTCCATTTCTAGCATATGGACACCTGGCCTCTGCTTGAGCACTTAGGTGACAGGAAC120.10914051841746249No Hit
GACCAATATGGTGAAACCCCGTCTCTGCTAAAAATACAAAAAAATTAGCTGGGTGTGGTGGTGGGTACCTG120.10914051841746249No Hit
GAAATGACCACGTGAAATTTGCCTCTGTCCAAACATTTCATCCGTGTGTATGTGTATGTGTGTGAGTGTGT120.10914051841746249No Hit
CCTGCAAGATGGACACGAGCCACAAGCTGCACTGTGAACCTGGGCACTCCGCGCCGATGCCACCGGCCTGT120.10914051841746249No Hit
GACTATCTGTTCATGAGAGAAATTTTCTGTAAGCTTGCTGTTTTACAGGGGATTTATCAATAATTGATTTT110.10004547521600728No Hit
AGCCAGCTGAGGGGAAGTTGCTGGTGAGTTTCCTTTTCTCCATTTCTAGCATATGGACACCTGGCCTCTGC110.10004547521600728No Hit
GCTAAAGACTTCCCTGTCTGCTCACATCTGGTCTGTTCCCCAGTATCCTCACTGTATTAAAGTTGTTTCTT110.10004547521600728No Hit
AACCAGACTCAGAAAGAAAACCTCAGGAAATTCTACAAGGGCAAGAAGTACAAGCCCCTGGACCTGCGGCC110.10004547521600728No Hit
GTGAATACATGGATAAAAATAATGTGAGGCGACAAGCAACAACAATCATAGCTGATAATATTATATTTCTG110.10004547521600728No Hit
CCATTAAAGAGATCCTGGGGACTGCCCAGTCAGTGGGCTGTAATGTTGATGGCCGCCATCCTCATGACATC110.10004547521600728No Hit
CCTCAGTGTATAAATTTATCCTCACACCTGAAATTCTTTTTCTGAACTAAAAATGTATGTGGCTTATCTTT110.10004547521600728No Hit
CAAACCATTTTATTTTTTGGGCCATTACCCCATACCCCTTATTGCTGCCAAAACCACATGGGCTGGGGGCC110.10004547521600728No Hit
GGAATAAGGAATGTGCCATACCGAATCCGTGTGCGGCTGTCCAGAAAACGTAATGAGGATGAAGATTCACC110.10004547521600728No Hit
ATCCAGCTGCCTATGAAAATGACAAATGAGCAACGCATCCGGATGACGGTTCCCTGTCTCTGAAAGACCTT110.10004547521600728No Hit
GTACTGAGTATCTCAATGATTTCTTCATCCAACAAGTAGTTATTGTGAGTCTTGCTCCACGGATGGAGTAG110.10004547521600728No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10004547521600728No Hit
CCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGTATGAAGATCGGGCGGATTGAG110.10004547521600728No Hit
CATCAAAGCCCTCAAGGAACCACCAAGAGACAGAAAGAAACAGAAAAACATTAAACACAGTGGGAATATCA110.10004547521600728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)