FastQCFastQC Report
Tue 7 May 2024
EGAF00002877647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60918
Total Bases4.3 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10621.7433270954397715No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA4790.7863028989789553No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA4460.732131718047211No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3440.5646935224400013No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC3020.49574838307232677No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.49082373026035No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT2800.45963426245116384No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG2270.37263206277290783No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.3463672477756985No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC2100.3447256968383729No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA2020.33159328933976817No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG1910.31353622902918676No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG1630.26757280278407036No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA1590.2610065990347681No Hit
TTCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.2527988443481401No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC1520.24951574247348896No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA1500.2462326405988378No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT1470.24130798778686102No Hit
TTCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.2347417840375587No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG1410.23145868216290752No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA1340.21996782560162842No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.21668472372697722No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATA1300.21340162185232608No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.20026921435372141No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT1200.19698611247907025No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC1080.1772875012311632No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT1070.17564595029383762No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTG930.15266423717127942No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAAT920.15102268623395385No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT880.1444564824846515No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC880.1444564824846515No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAA830.13624872779802358No Hit
AACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT700.11490856561279096No Hit
AATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC670.10998391280081421No Hit
TCCCTACACGACGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.10834236186348861No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA640.10505925998883746No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAA630.10341770905151187No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAA610.10013460717686071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)