FastQCFastQC Report
Tue 7 May 2024
EGAF00002877655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27526
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14325.20235413790598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.247039162973189No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA530.1925452299643973No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.18891230109714452No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.14531715469011117No Hit
AGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAG390.1416842258228584No Hit
TTCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.1235195814865945No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG340.1235195814865945No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT320.11625372375208894No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA310.11262079488483616No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA290.10535493715033059No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT280.10172200828307781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)