FastQCFastQC Report
Thu 9 May 2024
EGAF00002877672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291342
Total Bases162.6 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59820.26106971373107984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45420.19822444663433045No Hit
GTATTATTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC25000.10910636648741219Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)