FastQCFastQC Report
Wed 8 May 2024
EGAF00002877688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529117
Total Bases37.5 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18870.35663189804901374No Hit
ATATAGTACCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT11580.21885518703802753Illumina Single End PCR Primer 1 (100% over 39bp)
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC6980.1319178933959786No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA6920.13078392869629968No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC5780.1092385994024006No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT5750.10867161705256116No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA5560.1050807288369113No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA5550.10489173472029815No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT5550.10489173472029815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)