FastQCFastQC Report
Tue 7 May 2024
EGAF00002877699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820
Total Bases200.2 kbp
Sequences flagged as poor quality0
Sequence length71
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61221.70212765957447No Hit
GGTATTTCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATC411.4539007092198581No Hit
GGACCTGTAAACTTCATGGTTCGGCTTTTTGTGGTGATTGTGATGTTTGC371.3120567375886525No Hit
GTCTCTGCACAGAAACTTGAACTCCAGGATGGAATTCTTCCTCCTCTGCT351.2411347517730498No Hit
GCTTTACCCTGCTGCAGTAGATACCATTGTTGCTATCATGGCAGAAGGAA321.1347517730496455No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC301.0638297872340425No Hit
GCAGCACATTGTGGAGTGTGATTCTTAATTCTTCATTGAGTTTGTCAATA291.028368794326241No Hit
CCTGGCACAGTCACTTCACAAGTTGTCTACCGCCTGTCCAGGAAGGGTTC280.9929078014184398No Hit
GGAGACCATTGCATCATTGGCCGCACACTGGTGGTCCATGAAAAAGCAGA280.9929078014184398No Hit
GTGATTACTTTTGCACCAACCTAATACATAGTTTCAAGGTATCTTCCCAC270.9574468085106382No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA270.9574468085106382No Hit
CTAGTATGTTGGTCTGGTGACCTGGTTACATTTTATTATACACATTATTG260.9219858156028369No Hit
GTGTATGCCCTGCAAGTTGGCTGTCTATGAGCATTTAGAGATTTAGAAGA250.8865248226950355No Hit
GGCCAAGGCAGGAGGATTGCTTGAGGTCAGGAGTTCATGACTGGCTTGGG240.851063829787234No Hit
CTTTCCCTACCGTGTTTGATAAATGTTGTCCAGGTTCTATTGCCAAGAAT230.8156028368794326No Hit
ACTATGTGTAGACAGGTTTTATATTATAAAGTATGCATTCTTATCACCTA230.8156028368794326No Hit
GCCTCTGAGGCTGCGTGCCTGATCGTGTCTGTAGATGAAACCATCAAGAA230.8156028368794326No Hit
AGGGACCATCAGTTCCCTGCAAAAAGACCCAGGCTATCAGAACCCCAGGG230.8156028368794326No Hit
CACCGGACACCATCTCCTATGCCTTGGAGACCTTCTGTCACTTGGCTCCC220.7801418439716312No Hit
GCATGGAGAGCTACATTTCATAATTTGAGGGAAAATTTCTAACAGATGCC220.7801418439716312No Hit
GCATCCCACCGCCCTACGACAAGAAAAAGCGGATGGTGGTTCCTGCTGCC210.7446808510638299No Hit
GCTTCAGAGGTGGCCCCACCTGAGCCCCCACCCGGGAGCAGTGTCCTGTG200.7092198581560284No Hit
ATGTATTAATGAAGTGATTAATTGGCTCCATATTTAGACTAATAAAACAT200.7092198581560284No Hit
GTTATTATAATAATGGGTAATTTGTCAATAAAGCATTCCTTTGGGGGAAA200.7092198581560284No Hit
GCCGCCAGTGTTTCTCAGGGATGTGACTGAGGCCCAGGAGGGACCTGTGA190.6737588652482269No Hit
TGCCCAGAAATACAACTGCGACAAGATGATCTGCCGCAAGTGCTATGCTC190.6737588652482269No Hit
GTAAAGGCAGACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGC190.6737588652482269No Hit
GGGTGTGGTAGAGCATGCCTGTAGTCCCAGCTACTCAGAAGACTGAGGTG190.6737588652482269No Hit
GGAGAATCGCTTGAACCCAGGAGGCAGAGGTTACAGTGAGCTGAGATTAT190.6737588652482269No Hit
GCCTAGAGGAGCGGTTCAAGATGACTATACCACTGCTTGTCAGACAAATT180.6382978723404255No Hit
TCTACATCCTGTGCTTTTCTCCTAGTTTCTCTCCACCCTGGAACATTCGA180.6382978723404255No Hit
GCTACTAGGGAGGCTGAGGTAGGAGAATCGCTTGAACCTGGGAGGCGGAG180.6382978723404255No Hit
CCCCTCCACCCCATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCT170.6028368794326241No Hit
GATATCAAGTGTGTACTAAATGAAGGCATGCCAATTTATCGTAGACCATA170.6028368794326241No Hit
GAACATAGATATGTATTCAGCTTGTCTTCAAATACGGCCAAGCAGAAAAT170.6028368794326241No Hit
CCTTTAGAAATAAGAGGGCCATATGACGTGGCAAATCTAGGCTTGCTGTT160.5673758865248227No Hit
CCATTCCCTTCAGCAAGCCTGTAAAAGTTTATATAATGCCCAAACCTGCA160.5673758865248227No Hit
CATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGT150.5319148936170213No Hit
GGATCACGTAGACATGTAACCCAGCAGCAGTTTGCTTCTGTTGTCCACTG150.5319148936170213No Hit
GGATTCATATGCAGAAGCAAGACCTAAGCAAACTACAAACCAGGAAAATG150.5319148936170213No Hit
GCTTTGGCGTCCCCGTCTTCGGCCACGTGCAGGTTGCTATCAATACCCTT150.5319148936170213No Hit
GTGGAGAGTGGAGCCATGACCAAGGACCTGGCGGGCTGCATTCACGGCCT150.5319148936170213No Hit
GCTATTAGCCAGGATCATGGTGTAATAAGACATAACGTTTTTCCTTTAAA150.5319148936170213No Hit
CCAACATCATGGAGGTCCCTGGATTGAAAAAGGAGCCTCTCCCACTCCTC140.4964539007092199No Hit
AGTCTTTGTAGCCACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.4964539007092199No Hit
CCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAG140.4964539007092199No Hit
GTTCTAGAGGGCATGGAGGTGGTGCGGAAGGTGGAGAGCACCAAGACAGA140.4964539007092199No Hit
TGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAG130.46099290780141844No Hit
CTCCCCTCTGACCCTCAGGCTTTCCCAGCTTCCTCCCCTTGCTACAGCCT130.46099290780141844No Hit
GCTTTGGAGCCCAGCTGGCGCATATCCAGGCGCTGATCAGCGGTATTGAA130.46099290780141844No Hit
GAGATTTTTTTCTGCGTTGTTTAATGCCTGAAATCCAAGTCTTCCTCCAT130.46099290780141844No Hit
GGGGTGTGGCAGTGGTTTTGGCAAACGCTAAAGAGCCCTTGCCTCCCCAT120.425531914893617No Hit
GTATTCATTTGCTTGAAAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.425531914893617No Hit
ACTTTGGCATAGCGGCCTCCCTGGTCAAGATGGGCCGGCTGGAGGGCTGG120.425531914893617No Hit
GGCAAAGTTAAGCTTGATGATGGTTAAAATCGGTTTGATAGCACCATGGG120.425531914893617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.425531914893617No Hit
GAATGTTGCTGCAGAGACTGAATGACATGCAACAGGTGAAGGTTTATACG110.3900709219858156No Hit
CTTCAAGAGAATCAGCAAGAGAATTTAAACAAGTCTGCATTTCATATTAT110.3900709219858156No Hit
ATGTATATTAATTAAAACATCTTAATCCAGAAAAAAAAAAAAAAAAAAAA110.3900709219858156No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.3900709219858156No Hit
AATTTGATGTACACAACATTAAAAGTACTGACACCTGAGAATTTCTGCTC110.3900709219858156No Hit
ATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGC100.3546099290780142No Hit
GTACCACACCTTCCGGCCAGCTGTCCTCCTGCTGATGTTCCTCAGTGTCT100.3546099290780142No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCT100.3546099290780142No Hit
CTGGTACCCAGTGATCCCCACCCCAGGATCCTAAATCATGACTTACCTGC100.3546099290780142No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA100.3546099290780142No Hit
GTCTTGTTAGCCATAAAGGAATGATTACATGAACCAGTAAGCTGGCTCAG100.3546099290780142No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.3546099290780142No Hit
TCACCCCTGTAATCTCTGCACTTTGGAGGATGAGGCAGGGTGGAATGCTT80.28368794326241137No Hit
GAAGCTGAGTCAGCTACGGGCAAGCGGGCAGCTGAAGATGATGAGGATGA80.28368794326241137No Hit
GATCAAGTGTCCTTTCCTACACATAGTTCTCTCTCAGTCTCTAGGAATCA80.28368794326241137No Hit
TTCATGAACAATCCAAGCATAATTGTTAAGAACACGTGTATTAAATTCAT80.28368794326241137No Hit
GGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAG80.28368794326241137No Hit
ATTTAATAAAGTTTGTCACAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAA80.28368794326241137No Hit
GATACCTCTTTACGGACTCCACTTATGACTCCCTAAAGCCCATGTCGAAG80.28368794326241137No Hit
GTGGAGGAAAGAGCTAGATGAACAGGTTGCTTTAAAGAAAAAAAAAAAAA80.28368794326241137No Hit
CATATGTCTCATTTTCATCTAAAATTAAATGGCAGGAAACAAGGACTGCA70.24822695035460995No Hit
GAAGATGAGAAACTTCAAGGCAAGATTAACGATGAGGACAAACAGAAGAT70.24822695035460995No Hit
GAGAGAAAGCAAAAAGTTAGAAAACAAAACAAAACAGAAGTAAGATAAAT60.2127659574468085No Hit
GACAGATGCTCAAGTGAGGCTGCAGGCGCGGTGTGGTGGGGCCGAGTGTG60.2127659574468085No Hit
GGGTTTGTGGAGCAGGCTGAGGCTCTGAAGGCCAAGGGAGTCCAGGTGGT60.2127659574468085No Hit
TTCCCTACACGACGCGGGGAACCAAGACATGGTAAAAAAAAAAAAAAAAA60.2127659574468085No Hit
GAAATGACAGCAGTGGTACTGCCTGGTAAAAGAATTGGTTCTGTGACCCG60.2127659574468085No Hit
GCTTTGTGGAACGCTGCCAGGAACTGGTGCGGAGTGGGCCAGACCTCCGA60.2127659574468085No Hit
CTCTTGAGGGCAATAAAAGTTGTCATGGTGTGTAAAAAAAAAAAAAAAAA60.2127659574468085No Hit
AGCTTGTCACCGTTCGAGCAGCGCGCCTATCCGCACGTCTTCACTAAAGG60.2127659574468085No Hit
CCCAGAGCCTGGGAAGGAGGCCGCTATGCAGGGTAGCACTGGGAACAGGA50.1773049645390071No Hit
GCTTTATTTTTCAATTAAAAGTGGTTTTCTCCTAAAAAAAAAAAAAAAAA50.1773049645390071No Hit
GGGCCCTCGGTGTGAAGATGAACCTTCCACCGTCTTCACTGCATCCTGGA50.1773049645390071No Hit
GTTCATAGTCAAAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1773049645390071No Hit
GGTGACTTTGCTTTTGTGACTGACAACACTTATACTAAGCACCAAATCAG50.1773049645390071No Hit
CCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAA50.1773049645390071No Hit
CATCATCATGCCTTCCAAATTGTGGGTGGCTCTGTGGGCGCATCAATAAA50.1773049645390071No Hit
CTTTTTGTTTTGTTTTTGCCAAATGTAACATGAAAGCAGATGCTGCAGCT50.1773049645390071No Hit
GGAAGCTTCCCAAGAGTAGCCTCAACCTGTGCTTCTGTGCATTATTCTGA50.1773049645390071No Hit
GTATTATTGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1773049645390071No Hit
ATTTAGAGATGGTGACATTCTTGGAAAGTACGTAGACTGAAATAAGTCAC50.1773049645390071No Hit
CCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCGGATCACGAGGTCA50.1773049645390071No Hit
GCCTGGCCAACATGGTGAAACCCCTTCTCTACTAAAAATACAAAAATTAG50.1773049645390071No Hit
GTCCGGAACGTCTATAAGAAGGAGAAAGCTCGAGTCATCACTGAGGAAGA40.14184397163120568No Hit
GCAGAGAAACCCATGTCCTCTCTCCATAGGGCCTGTTTTACTATGATGTA40.14184397163120568No Hit
GTCCTCATTGGCAGAAATTTAGATAAGGATATCCTTAAACAGCTGTTTAT40.14184397163120568No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA40.14184397163120568No Hit
GTATGGGCTAGAGCAGGTCTTCATCATGCCTTGTCTTTTTTAACTGAGAA40.14184397163120568No Hit
GGCCAGTTTGTGTATTGCGGCAAGAAGGCCCAGCTCAACATTGGCAATGT40.14184397163120568No Hit
AATTAACATCACTAGTGACACTAATAAAATTAACTTCTTAGAATGCAAAA40.14184397163120568No Hit
TCCCTACACGACGCGGGAATTATGTGACAACCTTTGTGTAATAAAAATTT40.14184397163120568No Hit
GCATTTCACCATCCAATGCAGTCCTGCATCCCATTAGATTAACCCTCTTC40.14184397163120568No Hit
GTATCTTGGCATTTGTATTGATAGTTAAAATTCACTTCCAAATAAATAAA40.14184397163120568No Hit
GCATGTGGGCAAAGCCTTCAATCAGGGCAAGATCTTCAAGTGAACATCTC40.14184397163120568No Hit
CCATGACCAAGATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGC40.14184397163120568No Hit
CTCTTAAATATATTAAAAACAATTTACATTTCAGGAAAAAAAAAAAAAAA40.14184397163120568No Hit
GCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCC40.14184397163120568No Hit
GCACTGAAGCCTGAGCAACAGAGCGAGACCCTGTCTCCAAAAAAAAAAAA40.14184397163120568No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCGGC30.10638297872340426No Hit
AACGCAAGGAGCGCGAGATGGTGGCCACACAGCGGGAGATGATGGACGCG30.10638297872340426No Hit
TTGCCTTCCTTTCTCCAGGACCAATAAAATTTATAAGAGAGAAAAAAAAA30.10638297872340426No Hit
GAATAACTAAAATCTCTTCTCATCCTTTTAAAAAAAAAAAAAAAAAAAAA30.10638297872340426No Hit
TTGCTGAGTTGTTCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10638297872340426No Hit
CGGTGAACCGAGATCGCACCATTGCATTCCAGCCTGGGCAACAAGAGCAA30.10638297872340426No Hit
GCACCTGTGAGGCCTGACGTGTCAGTTCCCAATAAATGCTCTTCTGATTT30.10638297872340426No Hit
TTTCTGGAGTGTGCCCAGAACCAGGGTGACATCAAGCTCTGTGAGGGTTT30.10638297872340426No Hit
GGCTTAGGAAAACATGAACACAGGGGCAACAGAGGCAAACTGTTGTTCGA30.10638297872340426No Hit
ACATTATGTTATTGGACTTCTTCTGACTTTGCTAAGACAATGAAGGCTAT30.10638297872340426No Hit
GTAATTTATAATGGCTTTGCAGTAATGTGTATCAGATAAGAAGCACTGGA30.10638297872340426No Hit
GGGTGACCGATCTGAGGACTTTGGTGTGAATGAAGACTTGGCTGATTCAG30.10638297872340426No Hit
GTTATTATAATAATGGGTAATTTGTCAATAAAGCATTCCTTTGGGGAAAA30.10638297872340426No Hit
CCGCACTACTTCACCTGAGCCACCCAACCTAAATGTACTTATCTGTCCCC30.10638297872340426No Hit
GAATAAAAGTGCACACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10638297872340426No Hit
AAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10638297872340426No Hit
ACCAAAGCCCCTCCTGTGTGTGTTAATAAAAGAATCATAAACAAAAAAAA30.10638297872340426No Hit
GATGATTTGGAGTTGTGCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10638297872340426No Hit
CCTATTTCAGTATTTATATAACTAGATTAGTGCTTTCTAGCAAACGGTTC30.10638297872340426No Hit
CCTTGGAATCTGTGGATTTGAAGATGGCTCCTGCCTTCTCACGTGGGAAT30.10638297872340426No Hit
CAAATATAAGGCAAAGAGGAACTGCTGGCCACGAGTACGGGGTGTGGCCA30.10638297872340426No Hit
TCATAGGGCCGGAGGGAGAGCCCTTCCGACGCTACAGCCGCACCTTCCCA30.10638297872340426No Hit
GTCAGTGCAGAGGTGCCCCCTGCAGATGTTCAATAAAGGAGACAAGTGCT30.10638297872340426No Hit
GGGGCCACCTACAGATGCTCCTGCAGTGGACACAGCAGAACAAGTCTATA30.10638297872340426No Hit
AAGGATATTATTGAATACCCAAATAAAACGAACTGATACATATTTCTCCA30.10638297872340426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)