FastQCFastQC Report
Tue 7 May 2024
EGAF00002877701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54663
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8311.5202239174578782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1730.3164846422625908No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA740.1353749336845764No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC680.12439858771015129No Hit
CTCTAGTGCCATGACTCACATTACTGAAGGAATTCAGGAGGACTCTTTAA670.12256919671441377No Hit
ATTATACATATACTATTTCCTTTTTATTTTTTTAAAATTTTTTTGCTTTT640.11708102372720121No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA610.11159285073998866No Hit
GTAAATACACTGAGTAAAGAGCTACATTCAGAGTTCTCAGAAGTTATGAA600.10976345974425114No Hit
GTATAATCCAGAGAGTGAACAAGATAAAAATGAGAAAAAGGGATCAAAAA600.10976345974425114No Hit
GTTTATATTAAAGGCTTCCCAACTGATGCAACTCTTGATGACATAAAAGA600.10976345974425114No Hit
TTATAATGCAGAGTGAGAACTTTCCCTACCGTGTTTGATAAATGTTGTCC590.1079340687485136No Hit
GAGGTGGGCTGATCATCTGAGGTCAGGGGTTCGAGGCCAGCCCTGACCAA580.10610467775277611No Hit
TGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATA560.10244589576130106No Hit
CCCCACCTCTGTGTGTGATGTAGCTTTCTCTCCCTCAGCCTGCAAGGGTC550.10061650476556354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)