FastQCFastQC Report
Tue 7 May 2024
EGAF00002877706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048
Total Bases74.4 kbp
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19318.416030534351144No Hit
GAATCCCTCAGAGAAGCATGTGCATACTCTACCCTGTTGCCTGGCCTGGA383.6259541984732824No Hit
CTTGCCCGTTGCGCAGTATATACTGATCCAGATGATAAACCAGTGAACTA282.6717557251908395No Hit
TTCCTTCCCAATAGGTGATCTTAATTACTTTCAGAATATTTTCAAAATAG262.480916030534351No Hit
GGTTTATGAAATCAGTAATTTTTGATATTTCCCCAGTTCTTTTTAATGGG232.1946564885496183No Hit
CTTTTACAACGCTTTCCCCTCATTCTTGATGTGGTGTAATTGAAGGGTGT222.099236641221374No Hit
GGGTTCAGGCTTCACCCACTGGAAAGAACCACCATCTGCTCTAACCATGT201.9083969465648856No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTC201.9083969465648856No Hit
GGATCCTGTCACACCTGGAGACCTACAGGAGGCCAGAGTGAAGGGGTCCG191.8129770992366412No Hit
GTTCTTATGTCTTCTGTTCAACATTGTACAGGATATTCAATCCAGTGCAG191.8129770992366412No Hit
GAAGTCTCTAATATATCATTTGTCTTTTTTCCAGTGAGGTAAAATAAGGC181.717557251908397No Hit
GATGGAGGAAGCATCTGAGTTTGAGACCATGGCTGTTACAGGGATCATGT171.6221374045801527No Hit
AGATTACACTGTATTAACTCTCGAGGAGTTTCCTCACCAATAAACAGACA161.5267175572519083No Hit
GCTTCTTTCCGAGAGATGTCAAGTCCTCAAGAATTTGATGGCTTCTTCTG161.5267175572519083No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA141.3358778625954197No Hit
GTTGTACAGGTAGCTGGTGGTGTTGGATTATCCCATTGAAGCTGGGACAG141.3358778625954197No Hit
GGCTGCATCTTCGTCCATGCAAGCGGCTTCATTGGCGGTCACCGCACCCG131.2404580152671756No Hit
GAGGATAGCAGCAGAAGAGAAGAAGAAGCAGGATGAACTGAAACGGATTG131.2404580152671756No Hit
ATTTTATTTGCTTCGTAATAAAGTTTATAAGGAAGAGCATCTCATACAAA121.1450381679389312No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG121.1450381679389312No Hit
ATTTTAATATAATACTAAAAATCCTAAAAAAAAAAAAAAAAAAAAAAAAA111.049618320610687No Hit
TAAGTCGAGGGACTTTATGTTTTTGAGGCTTTCTGTTGCCATGGTGATGG111.049618320610687No Hit
CCCACATTTTTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.9541984732824428No Hit
ACAATAAAGTATTTATTTTGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAA100.9541984732824428No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC100.9541984732824428No Hit
GCCGTAAATTGTTAATAAAATTGATGTTTCCATTTTAAAAAAAAAAAAAA90.8587786259541985No Hit
ACTGAAACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.8587786259541985No Hit
CATTCCTGCCACCCGACATCAAAGCTGGCGTGTGACCTTTCCAGCCATGC80.7633587786259541No Hit
CTGTGTTTCTCTGAATAGAGGTGTGGAACCTGAGGGGCCAGCAGGCCTCT80.7633587786259541No Hit
GAATAAATACCTTCTAATAAACATGATGGCGCAGTCCCAAAAAAAAAAAA80.7633587786259541No Hit
GCTCCCTGAGATGTGCTGTCCACTAAGCACTGCACAAACAAGCAATCAAA80.7633587786259541No Hit
GCACTCCAGCCTGGGCAACAGAGTGAGACTTCATCTCAAAAAAAAAAGAA80.7633587786259541No Hit
GGCTGTAGTATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.6679389312977099No Hit
GCCTAGATGGCACCACTGCACTCCAGCCTGGGCGACAGAGAGAGACTCCA70.6679389312977099No Hit
CTTTCCCTACACGACGCGGGGAAGATAATGCAGGAGTCATAGTGAACAAT70.6679389312977099No Hit
GTCATGTGAGCCTCGTGTCATCTCCGGGGTGGCCACAGGCTAGATCCCCG70.6679389312977099No Hit
GAGGTTGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAA60.5725190839694656No Hit
ATCAATAAAGTACCCTGTGCTCAACCAAAAAAAAAAAAAAAAAAAAAAAA60.5725190839694656No Hit
CCTGAACCAAAGTCCCAGAGCTGGGCTGGGACGTGACCTGTGCTGAGGGC60.5725190839694656No Hit
TTCCAGTATGGATCTGCTAATATGCACAGTAAATCCATGTCTTTGTTTGT50.4770992366412214No Hit
GCAGTATTCACTATAACTTAACACTACGAGAGACTTAAAAAACAGTTACT50.4770992366412214No Hit
AGACAAATGGATTCCCTTAGAAGTAAGATTTCTATTTGCAGGATGAGTTG50.4770992366412214No Hit
CTCCCCTTCAAATGCTGTAATTAACAACACTTAAAAAACTTGAATAAAAT50.4770992366412214No Hit
AGTTTAAGAAAATGGAAATGGGCAACAGTGAGTGAAGACATATTTTAGCA50.4770992366412214No Hit
TGCATGAACAGTATCAGTCGTCTGGGCTCATGCTGGGATGTCGCAGTGCT50.4770992366412214No Hit
CCTGAACGCTGTCGTTCTGTCTGTTTCCTCCATGCTTGTGAACTGCACAA50.4770992366412214No Hit
GTATTTTCAAGCTTCTGAACTTAGGCAAAATATTCATCGCAAAGTCTCTA50.4770992366412214No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA40.38167938931297707No Hit
GAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTTTGGATAAAAA40.38167938931297707No Hit
AAGACGTGCACCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTT40.38167938931297707No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA40.38167938931297707No Hit
GCCGTGAGTACAACACTGCACTCCAGACTGGGTGACAGAGCAAGACCATC40.38167938931297707No Hit
CACCAGAGTCTCCGTGCAGCGGACTCAGGCTCCAGCTGTGGCTACAACAT40.38167938931297707No Hit
GGTTTGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTAAAAAA40.38167938931297707No Hit
GTACCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.38167938931297707No Hit
GCATCATGTCCCACCGGGTCCCCTGCAGTCCCAGTGCACCTGCCTGCTCA30.2862595419847328No Hit
GTGCAGTTCACAACTTGAGCCTGACTGTACATCTCTTGGATTTGTTTCAT30.2862595419847328No Hit
GGTGTTCTAAATGTCTTAAGAACCTAATTAAATAACTGACTACAAAAAAA30.2862595419847328No Hit
GTCCTGGACTTTCAAAAACCTTGAATAAGTCTCCCACAGTTGTATAAATT30.2862595419847328No Hit
TAATAAATGTCGTAAAACAGATAAACAGAATTAAAAATGAAAATCACATG30.2862595419847328No Hit
AAGACGTGCACCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTT30.2862595419847328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.2862595419847328No Hit
AATTGTGACTGTAAACATTGTACTGTAAATGTTTTGTAGTTTTCCCCCAA30.2862595419847328No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC30.2862595419847328No Hit
GTGTTTCCATTATTAAAAATTGAAGTTATTAGTTAAAAAAAAAAAAAAAA30.2862595419847328No Hit
GTTGTACAGGTAGCTGGTGGTGTTGGATTATCCCATTGAAGCTGGGCCAG20.19083969465648853No Hit
GAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCTAAGGAGAAGCG20.19083969465648853No Hit
GCCGTGAGTACAACACTGCACTCCAGACTGGGTGACAGAGCAAGACCTTG20.19083969465648853No Hit
AATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACA20.19083969465648853No Hit
TCTTTTGAGGTCAGGAGTTTGAGACCAGCCTGGACAACATGGTGAAACCG20.19083969465648853No Hit
AAGACGTGCACCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATT20.19083969465648853No Hit
ACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19083969465648853No Hit
GAAATGTGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19083969465648853No Hit
CTTCTGTTTTACTTGCCATTTTCCTGCTCTGTTTTCTCTGTGACATGAAG20.19083969465648853No Hit
AAGACGTGCACCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTT20.19083969465648853No Hit
GACCTCAAGGCTCAGCTCAGGGAGCTGAATATTACGGCAGCTAAGGAAAT20.19083969465648853No Hit
CTTTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTG20.19083969465648853No Hit
AAGACGTGCACCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTT20.19083969465648853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)