FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17716.889312977099237No Hit
GAATCCCTCAGAGAAGCATGTGCATACTCTACCCTGTTGCCTGGCCTGGAGAGGTTCTTGTTCGCTTGTGC323.0534351145038165No Hit
CTTGCCCGTTGCGCAGTATATACTGATCCAGATGATAAACCAGTGAACTATGTCAAAAGCACTCTCAATAT282.6717557251908395No Hit
TTCCTTCCCAATAGGTGATCTTAATTACTTTCAGAATATTTTCAAAATAGATATATTTTTAAAATCCTTAA262.480916030534351No Hit
CTTTTACAACGCTTTCCCCTCATTCTTGATGTGGTGTAATTGAAGGGTGTGAAATGCTTTGTCAATCATTT222.099236641221374No Hit
GGTTTATGAAATCAGTAATTTTTGATATTTCCCCAGTTCTTTTTAATGGGGTCAATAATGGACATTCTAGT201.9083969465648856No Hit
GGGTTCAGGCTTCACCCACTGGAAAGAACCACCATCTGCTCTAACCATGTAGACTTATTGCGGCCTGGTTT191.8129770992366412No Hit
GTTCTTATGTCTTCTGTTCAACATTGTACAGGATATTCAATCCAGTGCAGAATGATAAATACATGTAAGAA181.717557251908397No Hit
GAAGTCTCTAATATATCATTTGTCTTTTTTCCAGTGAGGTAAAATAAGGCATAAATGCAGGTAATTATTCC181.717557251908397No Hit
GGATCCTGTCACACCTGGAGACCTACAGGAGGCCAGAGTGAAGGGGTCCGTTCTGTCCCTCACACTGTGAC181.717557251908397No Hit
GATGGAGGAAGCATCTGAGTTTGAGACCATGGCTGTTACAGGGATCATGTAAACTTGCTGTTTTTGTTTTT171.6221374045801527No Hit
AGATTACACTGTATTAACTCTCGAGGAGTTTCCTCACCAATAAACAGACAACCTCAAAAAAAAAAAAAAAA161.5267175572519083No Hit
GCTTCTTTCCGAGAGATGTCAAGTCCTCAAGAATTTGATGGCTTCTTCTGCAGCTATAACCACAAGGAACC151.4312977099236641No Hit
GTTGTACAGGTAGCTGGTGGTGTTGGATTATCCCATTGAAGCTGGGACAGCTGTTCCTTTCTCATAGTGAT131.2404580152671756No Hit
GAGGATAGCAGCAGAAGAGAAGAAGAAGCAGGATGAACTGAAACGGATTGCCAGAGAATTGGCAGAAGATG131.2404580152671756No Hit
GGCTGCATCTTCGTCCATGCAAGCGGCTTCATTGGCGGTCACCGCACCCGAGAGGGTGCCTTGAGCATGGC131.2404580152671756No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG121.1450381679389312No Hit
ATTTTATTTGCTTCGTAATAAAGTTTATAAGGAAGAGCATCTCATACAAAAAAAAAAAAAAAAAAAAAAAA121.1450381679389312No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT111.049618320610687No Hit
ATTTTAATATAATACTAAAAATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111.049618320610687No Hit
ACAATAAAGTATTTATTTTGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.9541984732824428No Hit
CCCACATTTTTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.9541984732824428No Hit
GCCGTAAATTGTTAATAAAATTGATGTTTCCATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.8587786259541985No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGC90.8587786259541985No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTTCCCGCTCGGGT90.8587786259541985No Hit
ACTGAAACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.8587786259541985No Hit
CTGTGTTTCTCTGAATAGAGGTGTGGAACCTGAGGGGCCAGCAGGCCTCTCTGAAGGCCTCCATGGAGCAA80.7633587786259541No Hit
GCTCCCTGAGATGTGCTGTCCACTAAGCACTGCACAAACAAGCAATCAAATTATGAATAAACATAATAAAT80.7633587786259541No Hit
GAATAAATACCTTCTAATAAACATGATGGCGCAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.7633587786259541No Hit
CTTTCCCTACACGACGCGGGGAAGATAATGCAGGAGTCATAGTGAACAATAAAGGCGAGATGAAAGGTTCT70.6679389312977099No Hit
TAAGTCGAGGGACTTTATGTTTTTGAGGCTTTCTGTTGCCATGGTGATGGTGTAGCCCTCCCACTTTGCTG70.6679389312977099No Hit
GCACTCCAGCCTGGGCAACAGAGTGAGACTTCATCTCAAAAAAAAAAGAAAAGAAAAGAAAAAAAAAGTGG70.6679389312977099No Hit
GGCTGTAGTATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.6679389312977099No Hit
GCCTAGATGGCACCACTGCACTCCAGCCTGGGCGACAGAGAGAGACTCCATCTCAAAAAAAAAAAAAAAAA70.6679389312977099No Hit
CCTGAACCAAAGTCCCAGAGCTGGGCTGGGACGTGACCTGTGCTGAGGGCTGTGAGAATGTGAAACAACAG60.5725190839694656No Hit
GAGGTTGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCG60.5725190839694656No Hit
ATCAATAAAGTACCCTGTGCTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5725190839694656No Hit
AGTTTAAGAAAATGGAAATGGGCAACAGTGAGTGAAGACATATTTTAGCACTGAATAGAATATTTTTAAAA50.4770992366412214No Hit
TTCCAGTATGGATCTGCTAATATGCACAGTAAATCCATGTCTTTGTTTGTTTTTCTATTAAAAAGCAAAAA50.4770992366412214No Hit
GCAGTATTCACTATAACTTAACACTACGAGAGACTTAAAAAACAGTTACTGCAAAAAAAAAAAAAAAAAAA50.4770992366412214No Hit
GTATTTTCAAGCTTCTGAACTTAGGCAAAATATTCATCGCAAAGTCTCTAGCGTCATATTTTTCTCACCCA50.4770992366412214No Hit
AGACAAATGGATTCCCTTAGAAGTAAGATTTCTATTTGCAGGATGAGTTGGGCAGGGAAAAGGGTCAGGGT50.4770992366412214No Hit
CTCCCCTTCAAATGCTGTAATTAACAACACTTAAAAAACTTGAATAAAATATTGAAACCTCAAAAAAAAAA50.4770992366412214No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.38167938931297707No Hit
GAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAA40.38167938931297707No Hit
GCCGTGAGTACAACACTGCACTCCAGACTGGGTGACAGAGCAAGACCATCCTGTCACAAAAAAAAAAAAAA40.38167938931297707No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTTCCCGCTCGGGG40.38167938931297707No Hit
GGTTTGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAA40.38167938931297707No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.38167938931297707No Hit
GTACCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.38167938931297707No Hit
GTCCTGGACTTTCAAAAACCTTGAATAAGTCTCCCACAGTTGTATAAATTGGACAATTTAGGAATTTTAAA30.2862595419847328No Hit
GTGCAGTTCACAACTTGAGCCTGACTGTACATCTCTTGGATTTGTTTCATTAAAAAGAAGCACTTTATGTA30.2862595419847328No Hit
CCTGAACGCTGTCGTTCTGTCTGTTTCCTCCATGCTTGTGAACTGCACAACTTGAGCCTGACTGTACATCT30.2862595419847328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.2862595419847328No Hit
TAAGTCGAGGGACTTTATGTTTTTGAGGCTTTCTGTTGCCATGGTGATGGTGTAGCCCTCCCACTTTGCTT30.2862595419847328No Hit
CATTCCTGCCACCCGACATCAAAGCTGGCGTGTGACCTTTCCAGCCATGCGATATTCCCCTTGGAAGATGC30.2862595419847328No Hit
CATTCCTGCCACCCGACATCAAAGCTGGCGTGTGACCTTTCCAGCCATGCGATATTACCCTTGGAAGATGC30.2862595419847328No Hit
GTGTTTCCATTATTAAAAATTGAAGTTATTAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2862595419847328No Hit
TAATAAATGTCGTAAAACAGATAAACAGAATTAAAAATGAAAATCACATGATCATCTCAATAGATGCAGAA30.2862595419847328No Hit
GGTGTTCTAAATGTCTTAAGAACCTAATTAAATAACTGACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2862595419847328No Hit
TCTTTTGAGGTCAGGAGTTTGAGACCAGCCTGGACAACATGGTGAAACCGCGTCTCTACTAAAAAAAAAAA20.19083969465648853No Hit
CACCAGAGTCTCCGTGCAGCGGACTCAGGCTCCAGCTGTGGCTACAACATAGGGTTTTTATACAAGAAAAA20.19083969465648853No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA20.19083969465648853No Hit
ACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19083969465648853No Hit
TGCATGAACAGTATCAGTCGTCTGGGCTCATGCTGGGATGTCGCAGTGCTCCTGTTGCAACACCTCCCAGC20.19083969465648853No Hit
GACCTCAAGGCTCAGCTCAGGGAGCTGAATATTACGGCAGCTAAGGAAATTGAAGTTGGTGGTGGTCGGAA20.19083969465648853No Hit
GAAATGTGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19083969465648853No Hit
GCCGTGAGTACAACACTGCACTCCAGACTGGGTGACAGAGCAAGACCTTGTCTCTAAACAAGGCAAAACAA20.19083969465648853No Hit
AATTGTGACTGTAAACATTGTACTGTAAATGTTTTGTAGTTTTCCCCCAATAAAATTTTTGGAAAAAAAAA20.19083969465648853No Hit
GGTTTATGAAATCAGTAATTTTTGATATTTCCCCAGTTCTTTTTAATGGGGTCAATAATGTACATTCTAGT20.19083969465648853No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCGTTCCCGCGCGGGG20.19083969465648853No Hit
GAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGAG20.19083969465648853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.19083969465648853No Hit
GCATCATGTCCCACCGGGTCCCCTGCAGTCCCAGTGCACCTGCCTGCTCATCCAGGGACCTGTCCTCACTG20.19083969465648853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)