FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15156
Sequences flagged as poor quality0
Sequence length71
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12178.029823172340986No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1500.9897070467141725No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT1270.8379519662179995No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA960.6334125098970704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG720.4750593824228029No Hit
GTGTGTGCCCAGGCAGACTTCGAGGGGTTCGTGCTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACA360.23752969121140144No Hit
AAATACTAGAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGTAAT350.23093164423330695No Hit
GCTTATTGGACACATGGATTTTCTTCAGATTTGCACTTACTGCTAGCTCTGCTTTTTATGCAGGAGAAAAG350.23093164423330695No Hit
CTGAGGTGTTGATGTGGTTTTATGTCGGAGAGATTATAGGCAAGCGGGGCATCATTGGCTATGATGTTTGA330.217735550277118No Hit
CATAGGGCCTGTTTTACTATGATGTAAAAATTAGGTCATGTACATTTTCATATTAGACTTTTTGTTAAATA330.217735550277118No Hit
GCTACAGAAGCTTCCAACAAGCATGTAGGCAGATACTCAAATGACATTCAGGAACTCTAATATTCATGGAA310.20453945632092901No Hit
GCAAGACATGTTGGATGGATTCATTAAAAAATTTGTTCTCTGTCCTGAATGTGAGAATCCTGAAACAGATT310.20453945632092901No Hit
GTTCTCACAGTTATTAACCAGACTCAGAAAGAAAACCTCAGGAAATTCTACAAGGGCAAGAAGTACAAGCC300.1979414093428345No Hit
ATATTGATATTGAAGCAATGCTTGAGGCTCCTTACAAGAAGACTTGCCTAACGATATCTCACCTCTACTCC290.19134336236474003No Hit
GTAAGAGACTCCAGCATGGCCTTCTGTTTGCCCCGCAGTAAAGTAACTTCCATATAAAATGGTATTTGAAA290.19134336236474003No Hit
GCCGAAGAGTGTGGTAGGTAACGGTCCTCAGCGCAAGGGTCATTTCGTCGCTGGGAAGGGACGGCCCTCGC290.19134336236474003No Hit
GGTCCACCCAGCAGCTGTTTGCCCAGAGCTGGAGCCTCAGCTTGAAGATGATGCTCAAGGTACTCTTCATG290.19134336236474003No Hit
GCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATA290.19134336236474003No Hit
GCCTAGCTGCTACAAAGAAGACATGTTTTAGACAAATACTCATGTGTATGGGCAAAAAACTCGAGGACTGT290.19134336236474003No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC290.19134336236474003No Hit
CTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC280.18474531538664557No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA280.18474531538664557No Hit
GTCTAAACCTGTGACCACCCCTGAAGAAATTGCACAGGTTGCTACGATTTCTGCAAACGGAGACAAAGAAA280.18474531538664557No Hit
ACTCCAGCCTAAGTGACAGAATGAGACCCTGTCTTTAAAAAAGAAAGAAAGAAAACCCTAAAAATTACAGT270.17814726840855108No Hit
AGACAGTTGATACTTACAACTAGATTATAAATTTGAAAGCAAAAGCCTTCATGTCTTTGTTCTCTCCCTAC270.17814726840855108No Hit
CAGTTCATACAACTCAGATTTAGTATTATTGTTTATCATAAAACATATTATGTTCCTAGTTTTAAAAAGAC270.17814726840855108No Hit
TGTATGGGATACTGGACTGTGTGAGCCACACGCCAGCCCTGCGGCTCTGGATATCAGCCATGAAGTGGGAC270.17814726840855108No Hit
GCACTACACTAGGGAATTTGCTCCTGTTCTGTCACTTGTCATGGTCTTTCTTGGTATTAAAGGCCACCATT260.1715492214304566No Hit
CCTTTTGGCCCTGCAGCATGTCATGCTCCCAGAATTTCAGCTTCAGCTTAACTGACAGACGTTAAAGCTTT260.1715492214304566No Hit
GAATATGGAGGCACCAAAGTAGTTCTAGATGACAAGGATTATTTCCTATTTAGAGATGGTGACATTCTTGG260.1715492214304566No Hit
GAGATAAGAAGAGAAAGGGCCAAGTGATCCAGTTCTAAACTTTGGGATATTTTTCTTCAATTTTGAAGAGA250.1649511744523621No Hit
CCTGAGAAGCTTCAAGAGATAGAGGAATTCAAAGAGAGGTTACGGAAGCGGCGGGAGGAGAAGCTCCTTCG250.1649511744523621No Hit
ACATTGGGTGACATTGTCTTCAAGAGAATCAGCAAGAGAATTTAAACAAGTCTGCATTTCATATTATTTTA250.1649511744523621No Hit
TAGCTAGAGAGGAATGTGCTGTGAGGCTAGTTACACAGAGGAAGGAAACTAACATTTACTAATCCTCTGCC250.1649511744523621No Hit
AATAATGGCTGTTCAGCAGAGAAACCCATGTCCTCTCTCCATAGGGCCTGTTTTACTATGATGTAAAAATT240.1583531274742676No Hit
AGTTGGTGGAAGGGCCCCCACCTCCACCTGAGCCACCAAAGACGGCAGTTGCCCATGCCAAAGAGTATATT240.1583531274742676No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC240.1583531274742676No Hit
GTATTTATGATGAAATGCAGAAAAAAAAGGAGGAAAATAATCCCAAATTGCTTTTGGGGAAAGACAGAAAG240.1583531274742676No Hit
GTGGTGAGCTAGTCTACGGACGCTGTATACCCTCCTCCACTTCCCGCTGCAGCCAATAAAGGCCGTTCCTA230.15175508049617312No Hit
CTCCTCCACTTCCCGCTGCAGCCAATAAAGGCCGTTCCTACCATAAAAAAAAAAAAAAAAAAAAAAAAAAA230.15175508049617312No Hit
ATCCTGGGGACTGCCCAGTCAGTGGGCTGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACAT230.15175508049617312No Hit
GTTTTCTTCAGAAGAAGGACTTCGGAATGCACTACAACAGGAAAATCATATTATAGATGGAGTAAAGGTCC230.15175508049617312No Hit
GTGTATGTATTTTTTTCAATGCCAAACCAGCTGTGATCCAATTTACATCCACATTTTAGGTCCAACAGCAA230.15175508049617312No Hit
GCACAAATTAGTTATATATGGGGATGGTAGTTTTTTCATCTTCAGTTGTCTCTGATGCAGCTTATACGAAA230.15175508049617312No Hit
GGTTGCTACGATTTCTGCAAACGGAGACAAAGAAATTGGCAATATCATCTCTGATGCAATGAAAAAAGTTG230.15175508049617312No Hit
TTTCTAGACAATAAAGATTTGTTTGTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.15175508049617312No Hit
AACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTA220.14515703351807865No Hit
GCCTCACAAAGTGACACAGTATAAGAAGGGCAAGGATTCTTTGTATGCCCAGGGAAGGAGGCGCTATGATC220.14515703351807865No Hit
CTAATGTACTACTAATTGAGCAACCTACGCACTCAGTTGACTACGTAAATCTGTCAGGTCTTTTGTGATTT220.14515703351807865No Hit
TTCATGAAGACTGAAGCCCCACAGTGGAATTGGCAAACCCACTGCAGCCCCTGAGAGGAGGTCGAATGGGT220.14515703351807865No Hit
CCTCCTTGCAGAGTGTTGTGCACAAGGAAGAAAGCCTGGGGCCCAAGAGACAGAAAGTTGGATTCCTGGGA220.14515703351807865No Hit
ATACAAAGTTAGCCGGGCGTGGTGGCACATGCCTGTAGTCCCAGCTGCTCTGGAGGCTGAGGCAGGGGAAT220.14515703351807865No Hit
GACAAAGGCAAGGCCCCTTTTCACTCTCCAGATTTCCTCCTACCTAATGGCCTACTGACCTCCCCTAGAGG210.13855898653998416No Hit
GTATACCCTCCTCCACTTCCCGCTGCAGCCAATAAAGGCCGTTCCTACCATAAAAAAAAAAAAAAAAAAAA210.13855898653998416No Hit
ATATTAGACTTTTTGTTAAATAAACTTTTGTAATAGTCAAAAATGCTTTCTCAGATGTTCTGAATATAGAA210.13855898653998416No Hit
ACCCTCAATGATGCAGGTTTTACCACTGCAAATAATATTGCCAACTTGAAAATCAGTTTATTGAATAAAGA210.13855898653998416No Hit
TCCCAGATCTGTGCAAAAGCAGTGAGAGATGCACTGAAGACAGAATTCAAAGCAAATGCTGAGAAGACTTC210.13855898653998416No Hit
GTATGAGAAGAAAAAGACCTCAAGAAAGCAACGAAAGGAACGCAAGAACAGAATGAAGAAAGTCAGGGGGA210.13855898653998416No Hit
GATACTTCCTGTTTTCTTGTTTCATATTTTCATGTTCAAAATTTAAGTTTTACATTTTTACTACTGTTAAT200.13196093956188967No Hit
CCTGTTTCTTGGCATGTCATGTGTATGCAGCTCTTCGGTTTCCTGGCTTTCATGATATTCATGTGCTGGGT200.13196093956188967No Hit
CCTGGACAGGTGTATGATGTGGAGGAGGTGGATGTGAAAGATCCTAACTATGATGATGACCAGGAGAACTG200.13196093956188967No Hit
AGGCTGGACTCAGCTACATCCGATACTCCCAGATCTGTGCAAAAGCAGTGAGAGATGCACTGAAGACAGAA200.13196093956188967No Hit
TCCTTGCAGAGTGTTGTGCACAAGGAAGAAAGCCTGGGGCCCAAGAGACAGAAAGTTGGATTCCTGGGATG200.13196093956188967No Hit
GGTTCTTGTTGGTGCACAGCACAAATTAGTTATATATGGGGATGGTAGTTTTTTCATCTTCAGTTGTCTCT200.13196093956188967No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA200.13196093956188967No Hit
AAATCGGAGTAGCCCTCGCGTTTTAGCAGTTTGCATATCAGAACGGAATGGAAAGTCCGGCCACAGCGGGT200.13196093956188967No Hit
TCAGAAGAGTGTACTGTCGCATGGGGAGTCTGTAACCATGCTTTTCACTTCCACTGCATCTCTCGCTGGCT200.13196093956188967No Hit
TAACAAAGGCGAGGAGAAGAAAGACGACGAGAACATTCCGATGGAGACAGAGGAGACACACCTTGAAGAAA200.13196093956188967No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT200.13196093956188967No Hit
GGACTATGGCAAGGAGTCTCAGGCCAAGGATGTCATTGAAGAGTATTTCAAATGCAAGAAATGAAGAAATA190.1253628925837952No Hit
GATAATACAGGAAAAACGGGGTTACTAGTAGCCGCCCATAGCCTGCAACCTTTGCACTCCACTGTGCAATG190.1253628925837952No Hit
GGCAGAGAGGGCACGGCCCCACACTGCCCCAGAGACATATAGGAGCCAGGCAAACACCAGGGCGAGTGAGC190.1253628925837952No Hit
TCCCTACACGACGCGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1253628925837952No Hit
CTATAATACTAGGTACTATATACTAGGATTGAAATTCTGTGTAACTGCTATAAACGTTTTATTAAAGTTAT190.1253628925837952No Hit
TCCCTACACGACGCGGGACTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.11876484560570072No Hit
CCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTT180.11876484560570072No Hit
CTTACAGTCTGATCAGTTCTGCAAGATGCCCATCAACAACAAGATGGCCCAGTGGGGCAACAAGCCTGTAT180.11876484560570072No Hit
GAGCCGAGATCGCGCCACTGCACTCCAGCCTGGACAGCAGAGGAGACTCCAACTCAAAAATAAAATAAAAA180.11876484560570072No Hit
GTCATTGCATCCTCTACCAGCTGTTAATCCATCACTCTGAGGGGGAGGAAATGTTGCATTGCTGTTTGTAA180.11876484560570072No Hit
GTATAAGAAGGGCAAGGATTCTTTGTATGCCCAGGGAAGGAGGCGCTATGATCGGAAGCAGAGTGGCTATG180.11876484560570072No Hit
ATCCTAAACTGAGACAATGCCTTGTTTTTTTCTAATACCGTGGATGGTGGGAATTCGGGAAAATAACCAGT180.11876484560570072No Hit
ATTCGGCTGGTTAAAAGATGCACTAAACCTGATAGAAAGGAATTCCAGAAGATTGCCATGGCAACAGCAAT180.11876484560570072No Hit
GTGAGTCATGATGGCACCACTGCACTCCAGGCTGAGTGACAGAGCAAGACTCTGTCTGAAAAAAAAAAAAA180.11876484560570072No Hit
GAACTATGACACATGAATATGTGGGCTAAGAAATAGTTCCTCTTGATAAATAAACAATTAACAAATAAAAA180.11876484560570072No Hit
CTTCAGTTTTGTTAAGGAGATTTAGTTTTACTGCTTTGACTGGTGGGTCTCTAGAAGCAAAACTGAGTGAT180.11876484560570072No Hit
ATATTGGAATGGTGGTAATACGAGGAAATAGTATCATCATGTTAGAAGCCTTGGAACGAGTATAAATAATG180.11876484560570072No Hit
GTATATGTATCATCATAAAATATTTAAATAAAAAGTATCTTTAGAGTGAAAAAAAAAAAAAAAAAAAAAAA180.11876484560570072No Hit
TGTCTGTGTACTCAAGCAATAAAATGATTGTTTAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.11876484560570072No Hit
GTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCCGGGGGATG180.11876484560570072No Hit
GAACAATAAAGGCGAGATGAAAGGTTCTGCCATTACAGGACCAGTAGCAAAGGAGTGTGCAGACTTGTGGC180.11876484560570072No Hit
AACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAAATCTAGAGTAA170.11216679862760623No Hit
GGCCCATCATGTTCAGAGGTGAGGTCCGTCTGAATGTCGAAGAGAAGAAGACTCGAGCTGCCAGGGAAGGC170.11216679862760623No Hit
GGCCAACATGGTGAAACTTCATCTCAACTAAAAATACAAAAATTAGCCGGGTATGGTGGCGAATGCCTGTA170.11216679862760623No Hit
ATGTAATTGTAACTATACCTCTGGTTCCCATTAAAAGTGACCATTTTAGTTAAAAAAAAAAAAAAAAAAAA170.11216679862760623No Hit
CTCCTGAAAAGGCCTGTAGCCTGGCAAAAACGGCATTTGATGAAGCAATTGCTGAATTGGATACGCTGAAT170.11216679862760623No Hit
GGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACT170.11216679862760623No Hit
AACATTTCCATTGTGTTAAGTGTAAAAAGGTCCAGACATGATCATAAAATTTAAATTTTATACAATTAAAA170.11216679862760623No Hit
GCCCAGTAATGTATGTCTACTGGTGGGAGACTGTGAGGATCCCAGGATTCAGTATTCCTGGCCCAGAGGGC170.11216679862760623No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC170.11216679862760623No Hit
ATTTTAAACCAGCCCATCTTCCCCGCTGCAGTTGGAACCGTTCTACGTGGATTTGGGATCAACCAGCAGGC170.11216679862760623No Hit
GGGCAGATCACCGGAGGTCGGGAGTTGGAGACCAGCCTGAGCAACGTGGAGAAACCCCATCTGTACTAAAA170.11216679862760623No Hit
GTATTGTACCCTGTAAAACTGTGTGTTTGTGTGCTTTCAAAGATGTTGGGATTTTATTTATCTGGGGACAG160.10556875164951175No Hit
ATCGGAAGCAGAGTGGCTATGGTGGGCAGACAAAGCCAATTTTCCGGAAGAAGGCTAAGACCACAAAGAAG160.10556875164951175No Hit
TCTGAAGGCTATTGAATGCAACTTACAATGCTTAATAAAAATCTTTATTCTTTTAGTATAAAAAAAAAAAA160.10556875164951175No Hit
GGGTGTTATAGGATCACATTGACAAAAGTACCATGGAGTTTTATGAGTCAGCATATTTTATTGTTCTTATT160.10556875164951175No Hit
TTCCAGGCCCATGGAAACTTGGATATGGGTAATTTGATGACAAATAATCTTCACTAAAGGTCATGTACAGG160.10556875164951175No Hit
GGACGCCATGGCAGGATGGAGAGGACTTGGTGGATTTTTGTTTCTTGCCTGACCTCAGTTTCATGAAAGAA160.10556875164951175No Hit
GTTTAAACTGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10556875164951175No Hit
ATTCACAGCCGTGCACGCGCTCAAGCCACCAGGGTGCGAGATGCCAGCTCTGGAGTTCTCGGTTGTTGTAG160.10556875164951175No Hit
GCGCCAGCCCTGTCCGTCTTCACCCATCCCCAAGCCTACTAGAGCAAGAAACCAGTTGTAATATAAAATGC160.10556875164951175No Hit
CTCAAGAAGAAACGCAAGAAATGTTAGAAGAAGCAAAGAAAAATTTGCAAGAAGAAATTGACGCCTTAGAA160.10556875164951175No Hit
TTCCCTACACGACGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10556875164951175No Hit
ATAACATACATGTCAATATCACTGTTTTTAAATAAAATATACTTTTATACAGAAAAAAAAAAAAAAAAAAA160.10556875164951175No Hit
CAACATGATGCTGCCCATTCCACTGAAGTTCTGAAATCTTTCGTCATGTAAATAATTTCCATATTTCTCTT160.10556875164951175No Hit
GCTTCCTTAAACACTGGTGATTCTCTGTCTCGTCTGTAAAATGCACATCACCACCTACTTGAGTGTTGAGG160.10556875164951175No Hit
GTCCAACAGAAGTACGTGCAAGGGGTGTATGGAGAAGATAGAAAAGGGCCAGGTGCGCCTGTCCAAGAAGA160.10556875164951175No Hit
GCAGGAAACAAGTTTCACAAAAGAAGCCTACAAGAAGTACATCAAAGATTACATGAAATCAATCAAAGGGA160.10556875164951175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)