FastQCFastQC Report
Tue 7 May 2024
EGAF00002877769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16469
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4732.8720626631853787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1150.6982816200133585No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA480.2914566761794887No Hit
GTATTTTATAGGTCATAAAAAACACCATAATATAACGAATCTCATTTTCT460.27931264800534333No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC400.2428805634829073No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAA340.2064484789604712No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC330.20037646487339852No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.19430445078632583No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG300.18216042261218046No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG280.1700163944380351No Hit
GTTGAAAAGAACTTTGAAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAA260.15787236626388973No Hit
TCCCTACACGACGCGGGGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCA260.15787236626388973No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG250.15180035217681706No Hit
GGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGT240.14572833808974436No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA240.14572833808974436No Hit
GCATGGCATGGCATGGCATGGCATGGCATGGCATGGCATGGCATGGCATG240.14572833808974436No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG230.13965632400267167No Hit
TCCCTACACGACGCGGGTGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAA200.12144028174145365No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC200.12144028174145365No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA200.12144028174145365No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT190.11536826765438095No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAAAAAAA180.10929625356730828No Hit
GAATAACACTGCTGCAGATGACAAGCAGCCTTATGAAAAGAAGGCTGCGA180.10929625356730828No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10929625356730828No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG180.10929625356730828No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC180.10929625356730828No Hit
AGCTGCAACTGACTCATTGCCTCATGGTTTTTGTTTTTATTTTTATTTAT170.1032242394802356No Hit
TCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTT170.1032242394802356No Hit
GGTCAGAGCAGTATTCCAAAATGCGGAGTATGTTGTCTCCAGAACCTGAA170.1032242394802356No Hit
GGCATGGCATGGCATGGCATGGCATGGCATTGCATGGCATGGCATGGCAT170.1032242394802356No Hit
GAAAAAGACCACCCATTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAGG170.1032242394802356No Hit
CTTTCCCTACACGACGCGGGGAAGCGTTTACTTTGAAAAAATTAGAGTGT170.1032242394802356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)