FastQCFastQC Report
Tue 7 May 2024
EGAF00002877790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18245
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA6883.770896135927652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2281.2496574403946286No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC2281.2496574403946286No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG1640.8988764044943821No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250.6851192107426692No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA760.41655248013154295No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC730.40010961907371884No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATA530.2904905453548917No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA410.22471910112359553No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT380.20827624006577147No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA340.18635242532200602No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAA320.1753905179501233No Hit
GTATCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA290.15894765689229925No Hit
CTATATGTACTCTCTGGACTACTTTGGACAGAAGTAGGTTTTTGAATGTA290.15894765689229925No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA280.15346670320635789No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT270.14798574952041654No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC260.1425047958344752No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA250.13702384214853383No Hit
GTGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG250.13702384214853383No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC240.1315428884625925No Hit
AGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACTATGCA220.12058098109070979No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC220.12058098109070979No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTC210.11510002740476843No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA210.11510002740476843No Hit
TACTTATTTAATGAGATAATAAATGAAAACTTCTCAAGTCTAGTAACAGA210.11510002740476843No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA200.10961907371882709No Hit
GCATACACCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA200.10961907371882709No Hit
CTATTGATGAGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10961907371882709No Hit
TACTTGTATAGCAGTTTCTGCTTCACATTTGATTTTTTCAAATTTAATAT200.10961907371882709No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT190.10413812003288574No Hit
ATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTA190.10413812003288574No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT190.10413812003288574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)