FastQCFastQC Report
Tue 7 May 2024
EGAF00002877831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35750
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length66
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA362210.131468531468531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1920.5370629370629371No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.12307692307692308No Hit
GTATTGCGCTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC440.12307692307692308Illumina Single End PCR Primer 1 (100% over 38bp)
CTATTGCGCTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC400.11188811188811189Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)