FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16989
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6103.5905585967390663No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG930.5474130319618578No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG520.30608040496792044No Hit
CAGTTAGTAATGGTGCTGGGTAATAAGCATAAGACCAATCTCTTGCTGTTAAATCAGTTCTGTCCTTGGCA300.17658484901995408No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC270.15892636411795866No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGA260.15304020248396022No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA260.15304020248396022No Hit
GGCCTATGGTGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTATCGAGGAGC260.15304020248396022No Hit
GTCTATAAGAAGGAGAAAGCTCGAGTCATCACTGAGGAAGAGAAGAATTTCAAAGCCTTCGCTAGTCTCCG240.14126787921596326No Hit
GTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCA240.14126787921596326No Hit
CGTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG210.12360939431396785No Hit
CTTCAAATACAGTGTGTAGTTGAAGATGATAAAGTTGGAACAGATATGCTGGAGGAGCAGATCACTGCTTT200.1177232326799694No Hit
CATGGGGCCTGAACATGCAGGCAGCTCAGCCTAAGGGGAATCCAGTTGCTGCTCCTACAGGAGATGACCTC190.11183707104597092No Hit
TTCCCTACACGACGCGGGCTGATTAACAGCTAAGTAAATTCTATAGTACTGTACTGAAAATATAAAAAATT190.11183707104597092No Hit
AAAGAGAACTTGTTATTACTACTTTGGATGCTAATATGGAAAAAAATATGATTTACCAAGTCGATTAAGAA190.11183707104597092No Hit
GTGTAACATCACATAGTAATGTGTATTTTGTCTTGACTAAGTTGTGTAAAGGAATGTCTTTTTAATTCAGC190.11183707104597092No Hit
CACAGGCACTCTGCCTGCTTGGAGGTGTTCGCTGGCTCGCTCAGACTGACCTCAGCAGCCATGAACATGGG190.11183707104597092No Hit
TGCCAAAAGTGCGGCCACAAGGAGGCTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCG180.10595090941197245No Hit
GTATAGAAGATAGAAAAATATAAAGCCAAAAATTGGATAAAATAGCACTGAAAAAATGAGGAAATTATTGG180.10595090941197245No Hit
CACTTCAATTTTGCAATCGTTTTTACAGATGTTAAAAGACCTGGCTATCGAGTTAAGGAGATTGGCAGCAC170.10006474777797397No Hit
GCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCT170.10006474777797397No Hit
CAAGAAATACCGTGCCCTGAGGTTGGACGTGGGGAATTTCTCCTGGGGCTCAGAGTGTTGTACTCGTAAAA170.10006474777797397No Hit
TCCCTACACGACGCGGGATGGTCAGTAAAATATAAAAACATGTTTTTATGTTTTTAACCTTGCTTCTAATC170.10006474777797397No Hit
GTATTTACCAATAAAGGAAGCAGTTTGTGAGAAGCTAATATTTCCAGAAAGAATTGATCCTAACCCAGCAG170.10006474777797397No Hit
GTGTATACCTGTGTAACAAACCTGCACTTTCTGTACATGTGTCCCAGAACTTAAAGTATAATTTAAAAAAA170.10006474777797397No Hit
GTACAGATTTAAAAACAAATCTTGTTGCTGATTTGCCCATTTCTTTCCCTGCACTTTGTTACATCTGGGAT170.10006474777797397No Hit
GTCGTAAGGGCTGAGGATTTTTGGTCCGCACGCTCCTGCTCCTGACTCACCGCTGTTCGCTCTCGCCGAGG170.10006474777797397No Hit
CTCCACTGCTGCTGCTCCAGCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATG170.10006474777797397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)