FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11767
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4994.240673068751594No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA1431.2152630237103765No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG950.8073425681992011No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC800.6798674258519589No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.43341548398062374No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC450.38242542704172683No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC380.3229370272796805No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT360.3059403416333815No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA350.297441998810232No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC310.26344862751763404No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT280.2379535990481856No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT280.2379535990481856No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT280.2379535990481856No Hit
ACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATT260.22095691340188664No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC260.22095691340188664No Hit
TACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAAA260.22095691340188664No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT240.20396022775558764No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA230.19546188493243816No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA230.19546188493243816No Hit
ATACAAGCTTGTGATTTATGCTTACTCTAAGTGGAAATCAGGATTGTTATGAAGACTTAAGGCCCAGTATT220.18696354210928867No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.18696354210928867No Hit
GCTTTCATATAGCCAAGGCCCCATTAACTTGGGAATCTGGAAAACAGAAGACGTAAGTTTCTATCAGAGTT220.18696354210928867No Hit
CCTCTTACTGATTTACCCTACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACC220.18696354210928867No Hit
CAATATAGGAGCTGTGTCTACTATTAAAAGTGAAACATTTTGGCATGTTTGTTAATTCTAGTTTCATTTAA210.1784651992861392No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCA200.16996685646298973No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC200.16996685646298973No Hit
CCTTATAGTTAAGTGACTTGCCCAGGGTCACACAGCAAGTCAGTGGCACAGTGGTTGGAGAGGCCCTGGCC200.16996685646298973No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC200.16996685646298973No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA190.16146851363984024No Hit
CTTACACAGTTAGTCATGGAGAGTAACAGGCCTGCTGGTGAAACAGGTCACCCAAAATGGAGATGGCATCA190.16146851363984024No Hit
CCTATAAGTCTAATGGCTTTCAAATGTAATTTCCATTTGCTAATGGTGATCTTGCCACATCTGGCACGGAG180.15297017081669076No Hit
ATTGAAAACTAACATAATATGTTGAGGTTGCTTGGAAATGTACATAAAACTAAAATTTTCTGAATCGTGTG180.15297017081669076No Hit
ATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAA170.14447182799354125No Hit
CATGTACAGTGGGCACGGGAAGCTGGAATTCGTATGACGGAGTCTTATCTGAACTACACTTACTGAACAGC170.14447182799354125No Hit
GCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATT170.14447182799354125No Hit
CCAGCAGTCAAATTGCCTGAAGAGCATAGAATAAAATCTGAAAGTACAAACCATGAGCAACAATCTCCTCA170.14447182799354125No Hit
TTTCTAAACAGAATGGGCCGACTCAGTCACAGTAACTGTTGATCTCCATAGTAGAGCAACCCACAAAGACA170.14447182799354125No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14447182799354125No Hit
CCTTACAATCGTATACAACATTCACATGGCAATATTAGACAGTTAAGCACCCAATACCCATAGTTGACAAA170.14447182799354125No Hit
CATCAGACCATGGTGAATGTAAAGAATAGACCCTCATGAGGCTGGGCGCCGTGGCTCACACCTGTAATCCC160.13597348517039176No Hit
CTATAATACTAGAGGATACAATTAAAAAGGCATTATTTGAGACTTGATTCTACTTTTCCAGCAGAGGGCCC160.13597348517039176No Hit
CTCACTTATGCAGTATGTATTCTGTAAGTTCCTTTTACTCAGCATGTTTGTATGTTCATCAGTGTGTTTAA160.13597348517039176No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG160.13597348517039176No Hit
ATATAAAAATGTATGGGATGCAGCAAGAGCAGTTTTAAGAGGGAAGTATTTAGTAATAAACACCTACATCA160.13597348517039176No Hit
TTAAAGTACCTGTAGTGAGAAACTGATTTATGATCACTTGGAAGATTTGTATAGTTTTATAAAACTCAGTT160.13597348517039176No Hit
CCTGTTAACTTACATTTTATGGATTGGCACATTGTATTACTTACTGCAAGAGATATTTCATTTTCAGCACA160.13597348517039176No Hit
TACTTAAGCAGTAAGCGTATTTAGGTAAAAGCAGTTGAATTATGTTAAATGTTGCCCTTTGCCACGTTAAA160.13597348517039176No Hit
AATATGCACAGCACATGCAGTGCTAGCATCTAAACTAATACAATAAGCAATTACTAAAGCTACAGTGACTT160.13597348517039176No Hit
AAATTTGAGACGCAATACCAATACTTAGGATTTTGGTCTTGGTGTTTGTATGAAATTCTGAGGCCTTGATT160.13597348517039176No Hit
GAATTACCACGACCCCCAAGTGCTTCCGGCTCCAGGAAATAACCATTCATGTGTTTGCTGGAGGTCACACA160.13597348517039176No Hit
AAATTATACTAAACATTGATGTCCTTGATCATTTTATGTTCTCATATTACTTTTGATTCTACTATGATTGT150.12747514234724228No Hit
GAAGTAAGAGGTGGGACTAAACTGTTAAGAAGATAATAATTTAAAATAAAACTTCAGAATCCTTATTCTAG150.12747514234724228No Hit
CCTCATTAGGATGCATCACTGGCCTCGTGCACCGGCTTCACCAGCACTGCACCCTAATCAAAACAGGGGCC150.12747514234724228No Hit
AGGTGGATCTATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGTAAATCAGTAAGAG150.12747514234724228No Hit
CTGTTGTTGAACAACTTTGCAATGCCCTTTCATCTTCCATTAAATTAACCATAAAATGTAAAAACACAAAT150.12747514234724228No Hit
ATCTTATTCTATGGCATATGTATGGAAGGGTGTAAAGATTCTTTTGAAAGGTTTATTCACATTGTAGAACA150.12747514234724228No Hit
GAATAAAGTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCC150.12747514234724228No Hit
CTTTATTCAGATTACAATTAATTAGAGTGATTATGAATTAGTGTTCTACACCATTACTCAATTCTTAAAAA150.12747514234724228No Hit
ATCACAAGCAGTTTATAGTTTGACATATTCTTCTAGATCCTGTGTGTAGGCACAACATCCAATTTTATGGG150.12747514234724228No Hit
GTCTTAAGCAACAGCCCCAAATGCTGGCTGCCCCCAGCCAAGCATTGGGGCCGCCATCCTGCCTGGCACTG150.12747514234724228No Hit
TCCTATAACTAACTTGAGAAAAGCTGGAACTTAAGTTTAACAGTTATAGTTTACTCAGCTTCACTGTTACA150.12747514234724228No Hit
GTTTTGTGGACCACTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTAC140.1189767995240928No Hit
CACCAAATCACTACACAGGACAGCAAAGGGGTGAGAAGGGGCTGAGGGAGGAAAAGCCAGGAAACTGAGAT140.1189767995240928No Hit
CCTGTGAATATGTAAAACAAAACCATTATCTTTGAGGGAGTTTTTAATACCAATGACAGAACAGAGATTTG140.1189767995240928No Hit
ATCTTATCTCATAGTTTTTTCAGGGGTGCTTCTTGATTAGTAGGGAATTTTGAACACCTCTTTAAATACAG140.1189767995240928No Hit
ATCCAAAACAGTGTTCAATTTAACGTGGCAAAGGGCAACATTTAACATAATTCAACTGCTTTTACCTAAAT140.1189767995240928No Hit
ACATTCAGATGATTGTTTTTAAATGTTTTACTTTTGGTACAGTTGATAGACATCATAAACGATATCAAGCT140.1189767995240928No Hit
TATCTATAATGCCCTTCTATATGTGCTACCATAGATGTGACATTTTTGACCTTAATATCGTCTTTGAAAAT140.1189767995240928No Hit
CAACCGGACATGCGCATTCATAGCAGAAGGAAACCATCAAGAAGTGGAAGGCTGACCATGATGAGCAGTAG140.1189767995240928No Hit
GCTTTGGAATTTTCAAGTCAAAGTCCCAACCCTTTGCCTCTCCCCCTTTAATTTGCCTTTTCTCTTTTGAT140.1189767995240928No Hit
ATGTGTATCTTTCTGAGATGCTTTAAACGCTTTTTGTAAAAAGCATGTCTTCTGTTTGATATATGACAACT140.1189767995240928No Hit
GTTATAAAATCAGGAAATCCAAACAGCGATTTACACCGATTAACACCCCCTTTATATATTTTTTACAAAAA140.1189767995240928No Hit
TCCCCGAGGAGCTGGGCCCCTGCACAGGCACACAGGGCCCGGCCACACCCAGCGGCCCCCCGCACAGCCAC140.1189767995240928No Hit
AGTTTTAACTTTATAAGGCTTTTCAAAATGTGTTTTTCTGAGATACATTGTTATTTATTCATATCCAGAAA140.1189767995240928No Hit
TAATTGGACCATTTTCTATTTGTCTATTTTATACAAAAAGGCTACACAATTGTTACACTTTATTCAGATTA140.1189767995240928No Hit
GCTGCTCTTTAATATGGGGGTATTGAATAACTTACATAAAATATGTTAGCAAAACTTGAAAAAGTTAAAGC140.1189767995240928No Hit
GCATTATAGGAAATTGAAAAACACAAGCAAAGAACAAAGTCATTCACAATCACAAGCAGTTTATAGTTTGA140.1189767995240928No Hit
CAGTGGCACAGTGGTTGGAGAGGCCCTGGCCTTAACCACCAGCATAACATGGCCACCTCGCTTTTTTAACC140.1189767995240928No Hit
GACTGGGTCTAACTCAGGATATAAATGCTCAATCATGAAATTAAAAGGGAAGCAAGTCCATTATCATTATG130.11047845670094332No Hit
GCTGATGTTCTGAGCGAAGAAGCAATACTGAAATGGTATAAGGAAGCACATGTTGCTAAAGGCAAAAGTGT130.11047845670094332No Hit
GTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTTAA130.11047845670094332No Hit
CTAATACAGTATCTAACACAAAAGAAGCTTTAAAAAGACAGTTTCTTTCCTTATTTGATTAGCTAGAAGTT130.11047845670094332No Hit
GTGTTGTTGGTGTGCCCTGGTGCCGTGGTGGCGGTCACTCCCTCTGCTGCCAGTGTTTGGACAGAACCCAA130.11047845670094332No Hit
GCTTTAGCCCGGGAGTTTGAGATCAGCCTGGGCAATATCAGCAGACCCTATTTCTAACAAAAATAAAAATA130.11047845670094332No Hit
GGACTGGGCCATTCTCCTTAGAGAGAAGTGGGGTGGCTTTTAGGATGGCAAGGGACTTCCTGTAACAACGC130.11047845670094332No Hit
CAACAATCATTTTTCCCCTTCAGTTCTGCTGCTTTATACAACAGTGTGATTGACAGAAAATTGGATGGCAT130.11047845670094332No Hit
GATGTCTTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCAAGTTTGAAGAGAGATA130.11047845670094332No Hit
CATGTTTTCTAAACTAGATCTAGGAAGTGGAATGTGGAATCAATCCGTCCTCCTCCCCTTAAGGGCTAACC130.11047845670094332No Hit
GTCTTTCACACTCAGGTCTGTGGGAAGGAAAGGCAGTGAGACCAGGTGTGAACACATGCACGGAGACCAAT130.11047845670094332No Hit
CAAGTGATCTTTCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCATGCCCAGCCTGGG130.11047845670094332No Hit
CTAATTCATAATCACTCTAATTAATTGTAATCTGAATAAAGTGTAACAATTGTGTAGCCTTTTTGTATAAA130.11047845670094332No Hit
CTCTTCCTATATATATCCATAGTGGACTTATTCAGAACATAGATATGTATTCAGCTTGTCTTCAAATACGG130.11047845670094332No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTA130.11047845670094332No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTA130.11047845670094332No Hit
GTACAAAGGTTTCTAAATCCCTTCTTTTCTATGCACTTTTTTATTTAAGAGGTGGGGTCCCAGGTGGGAAC120.10198011387779382No Hit
GCTTAGCAAAGGAGAATACAATGGGATGAATCCACCCATTGATTTCAAGTTTTGCTTAGCCAGTCATCAGG120.10198011387779382No Hit
GGTATTACGTAACATGCACATACTCTAAAAAGTACTCATCCCTTGTTTTCTGCTCATTTCTTCCAGCCTGA120.10198011387779382No Hit
TGCTTAAGGGTTGCATACTTTTATTGGAGTAAATCTGAATGATCCTACTCCTTTGGAGTAAAACTAGTGCT120.10198011387779382No Hit
GGATTTTGGTCTTGGTGTTTGTATGAAATTCTGAGGCCTTGATTTAAATCTTTCATTGTATTGTGATTTCC120.10198011387779382No Hit
CTATACTTCACTACAAAGCTTGTCTATACAGTATCATATGACCTTTGATTATGAAAAAATCAGAATAAAAA120.10198011387779382No Hit
CTGCCACACCATCATGAACTACACAAGGACCTGTCCTAAGGGTCTGAATCCAGGGAAAGCTATTGCAGAGA120.10198011387779382No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACA120.10198011387779382No Hit
CTTGTAATCCTACTTGGGAGGTTGAGACAGGAGGATCACTTGAGGCTAGGAGTTGGAGGCTGCAGTGTACT120.10198011387779382No Hit
ATACCAATACTTAGGATTTTGGTCTTGGTGTTTGTATGAAATTCTGAGGCCTTGATTTAAATCTTTCATTG120.10198011387779382No Hit
ACCCATAGTTGACAAAATGTCCCACTGACCAGCATTCATTTAAATACATCCTTCGTATAGAGGGAGGAAAA120.10198011387779382No Hit
ATGCTATGTTGGCTAAAACTGGTGTCCATCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGA120.10198011387779382No Hit
GCCTCCACCCCTCCAGCCCATCCAGCAATCGCCCCCTTTCTGCCCTGGGGGCCCAGGATGTAGATATTGTA120.10198011387779382No Hit
GTGAAGAGACTCTGTTTGCTTGTGTTTGTTTGTTTGCAGTCTCTCTCTGCCATGGCCTTGGCAGGCTGCTG120.10198011387779382No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT120.10198011387779382No Hit
ATTCAGATTACAATTAATTAGAGTGATTATGAATTAATTAGTGTTCTACACCATTACTCAATTCTTAAAAA120.10198011387779382No Hit
GTGAAACCCCACCTCTACTAAAAATACAAAAAATAGCCAGGCATCGTGGCATGCACCTGTAATCCCAGCTA120.10198011387779382No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10198011387779382No Hit
CAAGAAATACCGTGCCCTGAGGTTGGACGTGGGGAATTTCTCCTGGGGCTCAGAGTGTTGTACTCGTAAAA120.10198011387779382No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10198011387779382No Hit
GCATTTACTATACTCCTTCCTAAGAGCACCAGATGGGGAACATGTTTTCTAAACTAGATCTAGGAAGTGGA120.10198011387779382No Hit
TGTCTATTTTATACAAAAAGGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAGAGTGATTAT120.10198011387779382No Hit
GATTACAATTAATTAGAGTGATTATGAATTAGTGTTCTACACCATTACTCAATTCTTAAAAATTAGAAATT120.10198011387779382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)