FastQCFastQC Report
Tue 7 May 2024
EGAF00002877923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12481
Total Bases886.1 kbp
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4593.6775899367037903No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA980.7851934941110488No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA560.448681996634885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG420.33651149747616377No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG400.3204871404534893No Hit
GGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGT380.30446278343081484No Hit
TGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTG280.2243409983174425No Hit
ACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCA280.2243409983174425No Hit
GCCCAGGGTCACACAGCAAGTCAGTGGCACAGTGGTTGGAGAGGCCCTGG270.2163288198061053No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG260.20831664129476804No Hit
CATATGACCTTTGATTATGAAAAAATCAGAATAAAAATCTTTAGTGACAT250.20030446278343084No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT250.20030446278343084No Hit
CATTAAACATTTGAGAGCTAACTATATTTTTATAAGACTACTATACAAAC250.20030446278343084No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA240.19229228427209358No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC230.18428010576075635No Hit
TGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGTGCTAGAAAAGG220.17626792724941912No Hit
TATGTGCATTATAGGAAATTGAAAAACACAAGCAAAGAACAAAGTCATTC220.17626792724941912No Hit
TTTCTACTCATTGCCATTTGTTAATACTAACCCTTAAAAAAAAGAAATAG220.17626792724941912No Hit
CTTTATATTTCTGGGACATTAAAATTCAAATCTCTGTTGAAAATGAAAAA220.17626792724941912No Hit
TACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCC220.17626792724941912No Hit
ATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTG220.17626792724941912No Hit
ATTATAAACAGAGGGGCAGACAGTTTTTGTTTGTTTTTAAGATGGGGTCT210.16825574873808188No Hit
CTGGTAAACTGTCCAAAACAAGGTTCCAAATAACACCTCTTACTGATTTA210.16825574873808188No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT210.16825574873808188No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT210.16825574873808188No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT200.16024357022674465No Hit
GAGTATAACTCTGAAGTCAGTGGGGTCAGTAAAAGCCTTATCAGACCATC200.16024357022674465No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC200.16024357022674465No Hit
GTTTATACATGGTGATAAAGAAAATTAAGAAAGATGAGGTGGCCAGGCAC200.16024357022674465No Hit
AAATAATGATGGGGCTAAGTTATACCCAAAGCTCACTTTACAAAATATTT200.16024357022674465No Hit
GGTGTTCGAGACCAGCCTGGCCAACATGGCGAAATCCTGTTTCTACAAAA200.16024357022674465No Hit
AATCTGGACATTCGAGGAATTGGCCGCTGTCACTGCTTGTTGTTTGCGCA200.16024357022674465No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA190.15223139171540742No Hit
CCTGACTCAGTATAGTTACTGGTACCACAGTGAGGTGAATAAAACGGGAT190.15223139171540742No Hit
GTCATTATATGACAATGTGAAGCCAAAGGAAGGTGAAGGATCTAAAGCTG190.15223139171540742No Hit
GGAAAGAGGTGTCTGGGGTCTCATTGTCAAATGGTGATTTGGTGAGTTTC190.15223139171540742No Hit
CGTCTCTACTAAAAATACAAAAAAAATAGCCAGGCTTGGTGGCAGGCGCC180.1442192132040702No Hit
CAAAAAGGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAGA180.1442192132040702No Hit
AGATATGGCAAAAAGTGACAAAGCTCGCTATGACAGGGAGATGAAAAATT180.1442192132040702No Hit
GCCTAGAATAGAACTGCTCAGCAGCAATAAAAACTAAATAAATTGTTACC180.1442192132040702No Hit
CAAGTGTCTTATATATGCAGATCTAATGTAAAATCCAGAACTTGGACTCC180.1442192132040702No Hit
GAGCTTGCTCCCTACGATGAGAACTGGTTCTACACGCGAGCTGCTTCCAC180.1442192132040702No Hit
ACAGTTCTTTTTAAAACACTGGAAGACTCATGTTAAACTCTAATTGTGAA180.1442192132040702No Hit
GAGCACGCTGGATGCCAAGGCCACAAAAGCCAAGAGCAAGAAGAACTGAG180.1442192132040702No Hit
GTTTGCAACTAAGTTTTACATTTTTCATTTTCAACAGAGATTTGAATTTT180.1442192132040702No Hit
CCTGTAATCCTAGCACTTTGGGAGGCCAAGGCAGGTGGATCACCTGAGGT180.1442192132040702No Hit
CTGTACATTAGTTCCAACTATCCCTATTTTACAATCTATAAGGCTCATGT180.1442192132040702No Hit
GTTATAGTGAATACTGCTACAGCAATTTCTAATTTTTAAGAATTGAGTAA180.1442192132040702No Hit
ATTTTAAAAATTTAATTAATTGGACTTCATCAAATTAAAATTTTTGTTCT170.13620703469273296No Hit
CTGTGGACAACTCGAATAAGTTTGACTTGTGTTTTATCTTAACCACCAGA170.13620703469273296No Hit
TAATACACGTGTTCTTAACAATTATGCTTGGATTGTTCATGAAAATTTCA170.13620703469273296No Hit
TTGATGATCTTATTAAATTACATCCAGAAAGTAAAGATGATAAACATGGA170.13620703469273296No Hit
TGCATATGATGTTTGAGTGCTGTTGTTTGAAATTATTTTTCTTTTCGGGT170.13620703469273296No Hit
CTTCTGCATCTTCCAATTGATTCCTTTACAAACTCTGCACACATACAACT170.13620703469273296No Hit
GTAATGGGATGTTGCAATACAAAAACACACAAGGAGGCTGGGCGTGGTGG170.13620703469273296No Hit
AATTTATACAATGTATATTGAGCACTAGTTCCTGTACAGCTTATTCTTAT170.13620703469273296No Hit
ATATTATAGACATATGTTAGAAAAGTCCTAGAAATGCACCCAATTTCCTT170.13620703469273296No Hit
AAATAATTTGAATAATATCATGGGACCCATATCAAGTGCTACTAATGATG170.13620703469273296No Hit
GGAGGTCAGGAGTTTGACACCAGCCTGACCAACATGGTGAAACCTTGTCT170.13620703469273296No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC170.13620703469273296No Hit
ATATTAGACAGTTAAGCACCCAATACCCATAGTTGACAAAATGTCCCACT170.13620703469273296No Hit
GTAACAAACATCCCTAATTGGATTCTAGAGAGGAAGCAGGGAAGGCCAGG170.13620703469273296No Hit
AGAGTGCATATATTTATATTTTGCCTGTTAAAAAGAAAGTGAGCAGTGTT170.13620703469273296No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT170.13620703469273296No Hit
GAATTAATGGACAGTCTGGTGAACTTCAAAAGCTTTTTGATGTATAAAAC170.13620703469273296No Hit
ATCTTTATCCCAATAAAGTTGATTATTGCAGGGGTTGAAAAAGTAATTTT170.13620703469273296No Hit
GTTATAGTTTACTCAGCTTCACTGTTACATCCTAGATGAGTATTGTATTC170.13620703469273296No Hit
CTAATACAGTATCTAACACAAAAGAAGCTTTAAAAAGACAGTTTCTTTCC160.12819485618139573No Hit
AATGTACACTGCTCTGTGAAATAAACCTGTAAAAAAGCAGACATCCTGTT160.12819485618139573No Hit
CCTTATTCTCAGCATCATTTGTTTAAAAAACAAAACAGCAAAACACCACT160.12819485618139573No Hit
CTTTCTGCCTTCTTCTTTATTCCATATTGGTCACACCCAGGATTTTCAAC160.12819485618139573No Hit
GTAGGATTACAAGCATGCGCCGACGATGCCCAGAATCCAGAACTTTGTCT160.12819485618139573No Hit
ATATTTGACAGACATCCTTGCAGTTTAAGATGACACTTTTAAAATAAATT160.12819485618139573No Hit
GCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATCG160.12819485618139573No Hit
GCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATA160.12819485618139573No Hit
GATAATTTTCTCTTCTTTAGGCAATGATTAAGTTAGGCAATTTCCAGTAT160.12819485618139573No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC160.12819485618139573No Hit
ATTTTATACAAAAAGGCTACACAATTGTTACACTTTATTCAGATTACAAT160.12819485618139573No Hit
GCACTTTGGGAGACCGAAGTGGGCGGATCACTTGAGGTCATGAGTTCGAG150.1201826776700585No Hit
GATGTGGTGGCCGTCACGCTTTCATGACCACTGGGCCTGCTAGTCTGTCA150.1201826776700585No Hit
ATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGTAA150.1201826776700585No Hit
ATAATGTAGAGGGGAAAAAAAGATCCAAAATATCCAAAATCCAAAATACA150.1201826776700585No Hit
GTGCACGCCTGTAGTCTCAGCTACTAGGGAGGCTGAGGCATGAGAATCGC150.1201826776700585No Hit
ATAATGCCCTTCTATATGTGCTACCATAGATGTGACATTTTTGACCTTAA150.1201826776700585No Hit
GCACTTTGGGAGGCCAAGGCAGGCAGATCACCTGAGGTCGGGAGTTCGAG150.1201826776700585No Hit
CACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAA150.1201826776700585No Hit
CATACAATGACTACTTTGAATACTTTGGACCAGATTTCAAGCTCCACATC150.1201826776700585No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC150.1201826776700585No Hit
GTAGAGGAGGAGGGAAGGAAGAAATATGCTAGGCTTAAATATTGCTCAAA150.1201826776700585No Hit
GCTCGAGTCATCACTGAGGAAGAGAAGAATTTCAAAGCCTTCGCTAGTCT150.1201826776700585No Hit
ATCTACAGCAAGTGATAAGGAAACTGGGCAAAAAAGGAAAAAAGCATACA150.1201826776700585No Hit
TGGTAAGAGCCCATTAGCTGCCAAGAGGGAATAAGGAATAAATTTCAAAA150.1201826776700585No Hit
GTATTGTGATTTCCTTTTAGGTATATTGCGCTAAGTGAAACTTGTCAAAT150.1201826776700585No Hit
GTATAGAACCTCTTGTTGGGCATGATGGCAAGGGACAAAGCTACAACTTG140.11217049915872125No Hit
GGAGAGCCATGCGGATTTTTAGAAGCTCCTGAGACCCGCCTGAAGATCAG140.11217049915872125No Hit
TCCCTACACGACGCGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGA140.11217049915872125No Hit
CTCTTATAAAATAGCTTTTGCCGAAACATAGTTTTTTTTTAGCAGATCCC140.11217049915872125No Hit
GATGTGCACTGCCATGCAGCATGGTAAGACGGGGTTTCACCATGTTGGCC140.11217049915872125No Hit
AGCTATACCTGAGGAACAGAGGCAGATAAATGTTATAACAACAATTTCGA140.11217049915872125No Hit
ACTTTTATTCCACTTACATGAATTTAATACACGTGTTCTTAACAATTATG140.11217049915872125No Hit
GTTTTTAAAAGGAGGATTTATTTGACAAGTTTCACTTAGCGCAATATACC140.11217049915872125No Hit
AGATTATCTTAAAACCAGAGTTCACTGATCTCACATTTTAAAAAGAAAAA140.11217049915872125No Hit
AGTCTCTACTAAAAATACAAAAAAAATTAAAAAATCAGCTGGACGTGGTG140.11217049915872125No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTG140.11217049915872125No Hit
GTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGG140.11217049915872125No Hit
ACCGAGCTCTGTGGGAAAAAAGAAAAACCTGCTCCCTTCGCTCTGCTGGA140.11217049915872125No Hit
GTGTCACCTGTATTTTACTTTGTATAGTCCCCATTGTTCATCTGAGTCTG140.11217049915872125No Hit
TACTTAACCTTAATGGAAACGCTGGAAATCTGCAGGGGGAGGGAGAGGGG140.11217049915872125No Hit
ACAAAGGTGTTTTTCTCTGTTAAGGAATTTATAAATAAAGGACTGTGTGT140.11217049915872125No Hit
GTTGTTGACTCGCATAATGCGAGAGATGTTGCGGAAACGAGCCAAGGAGC140.11217049915872125No Hit
GCCCCGAGTTGTTTCCTCGCTGATCGATTTCTTTCCTCCAGGTAGAGTTT130.10415832064738402No Hit
GTTATTAATCTGGACATTCGAGGAATTGGCCGCTGTCACTGCTTGTTGTT130.10415832064738402No Hit
GTTTCAGAGTTGAGAGTTGGTGGTGCAAATATGTATATAATGAACTGTAT130.10415832064738402No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG130.10415832064738402No Hit
AAGAAGAACTGAGGAGTGGTGGACCAGGCTCGAACACTGGCCGAGGAGGA130.10415832064738402No Hit
GGATGGGCCGGTGGGGCTGGTCAGGTCACAGTGTGAGGGACAGAACGGAC130.10415832064738402No Hit
CTACTATACAAACTACAGAGTTTATGATTTAAGGTACTTAAAGCTTCTAT130.10415832064738402No Hit
GGTGTGGTGGTACATGCCTGTAGTCCCAGCTGCTTGGGAGGCTGAGGCAG130.10415832064738402No Hit
GAGTTGTACGTGGAGGACTAATGAGACTTGTACAAGACACCACGGGGCTT130.10415832064738402No Hit
GGTAAGGAAACAGTTGGTCAACTCTGATACAAAGTATATATACAGTTCAG130.10415832064738402No Hit
GTTGGAAACAGCACATTGCTGATTTACACTGGATCTTGCCTTAGAAACCA130.10415832064738402No Hit
CAATACTAGCCATAAATACTTTTGCATTAATTTCTGAAGCTTTATAATGT130.10415832064738402No Hit
TCCGTGTCCGTGTAAAGGGTGGTGGTCACGTGGCCCAGATTTATGCTATC130.10415832064738402No Hit
GGGTAGTCTTCTCTGCCTTCTACCCCACCAGGCTACAACCATTTGAATGT130.10415832064738402No Hit
GTGCCTGTACTCCCAGCTACACAGGAGTCTGAGGCAGGAGAATGGCGTGA130.10415832064738402No Hit
GTGCATGTAATACTTTTGCAAGTGTTGGGGAGATCGGTATGTTTTGAAAA130.10415832064738402No Hit
TATCAGAAGTGTGTTTACCAACTTATTTTTGTTTGAAAGTGTGATTTTTT130.10415832064738402No Hit
TGCTAGTCCTAGATTACTTATTGATTTAAAAACAAAAAAACACAAAAAAA130.10415832064738402No Hit
CCTTCTGCAACATCTTAAGATCCACAAATGAAGGGATATAAAAATAATGT130.10415832064738402No Hit
GCCCAATATACAGAGAAAGGAGGAAAAAAGCTAGAATTCTATGCACTACT130.10415832064738402No Hit
GGTCTAACAGCCAAAATGTGCAGAGGAAATGAGCTCCTGCTGTCTTCCAT130.10415832064738402No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG130.10415832064738402No Hit
GAGTTGAGCACCCTCGCTGCTTCTCCGCTCTGCTCTTTCCTGCTTGCCCC130.10415832064738402No Hit
TGCTTGGATTGTTCATGAAAATTTCATAAGACATTAAACAAAGCTAGCCA130.10415832064738402No Hit
GGCAGACTTTGAAGGGTTTGTGCTGTAAGACCTAAAGTTCTTATGAGGTT130.10415832064738402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)