FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12481
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4493.5974681515904177No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG710.5688646743049435No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA520.4166332825895361No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380.30446278343081484No Hit
GGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTT350.28042624789680315No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC340.2724140693854659No Hit
ACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATT270.2163288198061053No Hit
GCCCAGGGTCACACAGCAAGTCAGTGGCACAGTGGTTGGAGAGGCCCTGGCCTTAACCACCAGCATAACAT260.20831664129476804No Hit
TGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTTT260.20831664129476804No Hit
CATATGACCTTTGATTATGAAAAAATCAGAATAAAAATCTTTAGTGACATAAGCCTTACAATCGTATACAA250.20030446278343084No Hit
CATTAAACATTTGAGAGCTAACTATATTTTTATAAGACTACTATACAAACTACAGAGTTTATGATTTAAGG240.19229228427209358No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT230.18428010576075635No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT220.17626792724941912No Hit
ATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAA210.16825574873808188No Hit
TGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGTGCTAGAAAAGGCAGCTAAAGGAAGTGAATCTG210.16825574873808188No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA210.16825574873808188No Hit
TTTCTACTCATTGCCATTTGTTAATACTAACCCTTAAAAAAAAGAAATAGGAAAAGAAAGGCTGCAATAAA210.16825574873808188No Hit
TACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATAT210.16825574873808188No Hit
TATGTGCATTATAGGAAATTGAAAAACACAAGCAAAGAACAAAGTCATTCACAATCACAAGCAGTTTATAG210.16825574873808188No Hit
ATTATAAACAGAGGGGCAGACAGTTTTTGTTTGTTTTTAAGATGGGGTCTCACTCTGTTGCCCAGGCTGGA200.16024357022674465No Hit
GGTGTTCGAGACCAGCCTGGCCAACATGGCGAAATCCTGTTTCTACAAAAAATACAAAATACAAAAAATTT200.16024357022674465No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA200.16024357022674465No Hit
CTTTATATTTCTGGGACATTAAAATTCAAATCTCTGTTGAAAATGAAAAATGTAAAACTTAGTTGCAAACA200.16024357022674465No Hit
GTCATTATATGACAATGTGAAGCCAAAGGAAGGTGAAGGATCTAAAGCTGGAGAATTGAATGCCAGATAAA190.15223139171540742No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT190.15223139171540742No Hit
GAGTATAACTCTGAAGTCAGTGGGGTCAGTAAAAGCCTTATCAGACCATCCATAGTTTTACAATGTGATCT180.1442192132040702No Hit
GCCTAGAATAGAACTGCTCAGCAGCAATAAAAACTAAATAAATTGTTACCTTAAGCTACTTTTAGAGCTAT180.1442192132040702No Hit
AAATAATGATGGGGCTAAGTTATACCCAAAGCTCACTTTACAAAATATTTCCTCAGTACTTTGCAGAAAAC180.1442192132040702No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC180.1442192132040702No Hit
AATCTGGACATTCGAGGAATTGGCCGCTGTCACTGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGG180.1442192132040702No Hit
GGAAAGAGGTGTCTGGGGTCTCATTGTCAAATGGTGATTTGGTGAGTTTCAGTCCGTTGCCTGAGGAAAGA180.1442192132040702No Hit
GTTTATACATGGTGATAAAGAAAATTAAGAAAGATGAGGTGGCCAGGCACGGTGGCTCACGCCTGTAATCC180.1442192132040702No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATC180.1442192132040702No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC180.1442192132040702No Hit
CCTGACTCAGTATAGTTACTGGTACCACAGTGAGGTGAATAAAACGGGATTTTCAGAAGTTAGCCTGAATT170.13620703469273296No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT170.13620703469273296No Hit
GTAACAAACATCCCTAATTGGATTCTAGAGAGGAAGCAGGGAAGGCCAGGTGCGGTGGCTCATGCCTGTAA170.13620703469273296No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC170.13620703469273296No Hit
GAGCTTGCTCCCTACGATGAGAACTGGTTCTACACGCGAGCTGCTTCCACAGCGCGGCACCTGTACCTCCG170.13620703469273296No Hit
ATTTTAAAAATTTAATTAATTGGACTTCATCAAATTAAAATTTTTGTTCTCTGAAAAACACTGGTAAGTAA170.13620703469273296No Hit
TAATACACGTGTTCTTAACAATTATGCTTGGATTGTTCATGAAAATTTCATAAGACATTAAACAAAGCTAG170.13620703469273296No Hit
ATCTTTATCCCAATAAAGTTGATTATTGCAGGGGTTGAAAAAGTAATTTTTTGTTTATTCTTCTAAGGTCT170.13620703469273296No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT170.13620703469273296No Hit
CTGTGGACAACTCGAATAAGTTTGACTTGTGTTTTATCTTAACCACCAGATCATTCCTTCTGTAGCTCAGG170.13620703469273296No Hit
CCTGTAATCCTAGCACTTTGGGAGGCCAAGGCAGGTGGATCACCTGAGGTCAGGAGTTCGAGAACAGCCTG170.13620703469273296No Hit
CTGTACATTAGTTCCAACTATCCCTATTTTACAATCTATAAGGCTCATGTCATAGTTCAGCACCAAAAAGA170.13620703469273296No Hit
TTGATGATCTTATTAAATTACATCCAGAAAGTAAAGATGATAAACATGGAAGTTATGAAGATGCTGTTCAC160.12819485618139573No Hit
CTTTCTGCCTTCTTCTTTATTCCATATTGGTCACACCCAGGATTTTCAACATATTGTGCTTCTGTAAGAAA160.12819485618139573No Hit
CTTCTGCATCTTCCAATTGATTCCTTTACAAACTCTGCACACATACAACTTAAGCCTCTGCTCAGGCTTAT160.12819485618139573No Hit
CTAATACAGTATCTAACACAAAAGAAGCTTTAAAAAGACAGTTTCTTTCCTTATTTGATTAGCTAGAAGTT160.12819485618139573No Hit
CTGGTAAACTGTCCAAAACAAGGTTCCAAATAACACCTCTTACTGATTTACCCTACCCATACATATCCCAA160.12819485618139573No Hit
GTTTGCAACTAAGTTTTACATTTTTCATTTTCAACAGAGATTTGAATTTTAATGTCCCAGAAATATAAAGA160.12819485618139573No Hit
GAATTAATGGACAGTCTGGTGAACTTCAAAAGCTTTTTGATGTATAAAACTTGATAAATGGAACTATTCCA160.12819485618139573No Hit
AATGTACACTGCTCTGTGAAATAAACCTGTAAAAAAGCAGACATCCTGTTAGCTGCAGGTTGATAAACAAA160.12819485618139573No Hit
ATTTTATACAAAAAGGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAGAGTGATTATGAATT160.12819485618139573No Hit
GGAGGTCAGGAGTTTGACACCAGCCTGACCAACATGGTGAAACCTTGTCTCTACTAAAAATACAAAAATTA160.12819485618139573No Hit
GATAATTTTCTCTTCTTTAGGCAATGATTAAGTTAGGCAATTTCCAGTATGTTAAGTAACACACTTATTTT160.12819485618139573No Hit
GTTATAGTTTACTCAGCTTCACTGTTACATCCTAGATGAGTATTGTATTCAAAAATACTGGGCCTTAAGTC160.12819485618139573No Hit
ATATTAGACAGTTAAGCACCCAATACCCATAGTTGACAAAATGTCCCACTGACCAGCATTCATTTAAATAC160.12819485618139573No Hit
CAAGTGTCTTATATATGCAGATCTAATGTAAAATCCAGAACTTGGACTCCATCGTTAAAATTATTTATGTG160.12819485618139573No Hit
AGAGTGCATATATTTATATTTTGCCTGTTAAAAAGAAAGTGAGCAGTGTTAGCTTAGTTCTCTTTTGATGT150.1201826776700585No Hit
AAATAATTTGAATAATATCATGGGACCCATATCAAGTGCTACTAATGATGGTGGAAGTGCTCCCAAGAAGC150.1201826776700585No Hit
ATATTATAGACATATGTTAGAAAAGTCCTAGAAATGCACCCAATTTCCTTCCATTTTACTTTCCTACATGG150.1201826776700585No Hit
GCTGCACCCAGCTCTGTAGGTGACTTTTAAATGATTATACAATGGAAATAACATTCATTGACATTTCTGTG150.1201826776700585No Hit
ATATTTGACAGACATCCTTGCAGTTTAAGATGACACTTTTAAAATAAATTCTCTCCTAATGATGACTTGAG150.1201826776700585No Hit
ATAATGCCCTTCTATATGTGCTACCATAGATGTGACATTTTTGACCTTAATATCGTCTTTGAAAATGTTAA150.1201826776700585No Hit
GTTATAGTGAATACTGCTACAGCAATTTCTAATTTTTAAGAATTGAGTAATGGTGTAGAACACTAATTAAT150.1201826776700585No Hit
GCTCGAGTCATCACTGAGGAAGAGAAGAATTTCAAAGCCTTCGCTAGTCTCCGTATGGCCCGTGCCAACGC150.1201826776700585No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA150.1201826776700585No Hit
GCACTTTGGGAGACCGAAGTGGGCGGATCACTTGAGGTCATGAGTTCGAGACTAGCCTGGCCAACATGGTG150.1201826776700585No Hit
GAGCACGCTGGATGCCAAGGCCACAAAAGCCAAGAGCAAGAAGAACTGAGGAGTGGTGGACCAGGCTCGAA150.1201826776700585No Hit
CGTCTCTACTAAAAATACAAAAAAAATAGCCAGGCTTGGTGGCAGGCGCCTGTAGTCCCAGCTACTTGGGA150.1201826776700585No Hit
GTAGGATTACAAGCATGCGCCGACGATGCCCAGAATCCAGAACTTTGTCTATCACTCTCCCCAACAACCTA150.1201826776700585No Hit
GTAGAGGAGGAGGGAAGGAAGAAATATGCTAGGCTTAAATATTGCTCAAAAGGAATTATTGGTGACTGAAA150.1201826776700585No Hit
GATGTGGTGGCCGTCACGCTTTCATGACCACTGGGCCTGCTAGTCTGTCAGGGCCTTGGCCCAGGGGTCAG150.1201826776700585No Hit
ACAGTTCTTTTTAAAACACTGGAAGACTCATGTTAAACTCTAATTGTGAAGGCAGAATCTCTGCTAATTTT150.1201826776700585No Hit
GTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTT140.11217049915872125No Hit
CTCTTATAAAATAGCTTTTGCCGAAACATAGTTTTTTTTTAGCAGATCCCAAAATATAATGAAGGGGATGG140.11217049915872125No Hit
ATAATGTAGAGGGGAAAAAAAGATCCAAAATATCCAAAATCCAAAATACATTTAATATAATGTAGAGGGAA140.11217049915872125No Hit
TACTTAACCTTAATGGAAACGCTGGAAATCTGCAGGGGGAGGGAGAGGGGAACTGTTATCTCCCAAGATTA140.11217049915872125No Hit
TGCATATGATGTTTGAGTGCTGTTGTTTGAAATTATTTTTCTTTTCGGGTCTTCAAAAATTCAAGAAAAGT140.11217049915872125No Hit
CACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTT140.11217049915872125No Hit
CCTTATTCTCAGCATCATTTGTTTAAAAAACAAAACAGCAAAACACCACTAAACGTGAACAAGAGAACAGT140.11217049915872125No Hit
GCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTCTTCAATC140.11217049915872125No Hit
AATTTATACAATGTATATTGAGCACTAGTTCCTGTACAGCTTATTCTTATTAGTTTGGATCCAACTATTCC140.11217049915872125No Hit
GTTTTTAAAAGGAGGATTTATTTGACAAGTTTCACTTAGCGCAATATACCTAAAAGGAAATCACAATACAA140.11217049915872125No Hit
ATTTCATGTTTTTGATCAAAAACTATTTGGGATATGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTG140.11217049915872125No Hit
ACTTTTATTCCACTTACATGAATTTAATACACGTGTTCTTAACAATTATGCTTGGATTGTTCATGAAAATT130.10415832064738402No Hit
GGCAGACTTTGAAGGGTTTGTGCTGTAAGACCTAAAGTTCTTATGAGGTTGTCCAAAACAAAGAAACATGT130.10415832064738402No Hit
GTGCATGTAATACTTTTGCAAGTGTTGGGGAGATCGGTATGTTTTGAAAAGAGTAATTTAACTTTTGGGTG130.10415832064738402No Hit
GCACTTTGGGAGGCCAAGGCAGGCAGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGACCAACATGGAG130.10415832064738402No Hit
GGGTAGTCTTCTCTGCCTTCTACCCCACCAGGCTACAACCATTTGAATGTCCATTCCTGTGGATACCCCAG130.10415832064738402No Hit
GGTAAGGAAACAGTTGGTCAACTCTGATACAAAGTATATATACAGTTCAGTATTGTCTCTGTTCATTTTGT130.10415832064738402No Hit
TCCCTACACGACGCGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC130.10415832064738402No Hit
AGATATGGCAAAAAGTGACAAAGCTCGCTATGACAGGGAGATGAAAAATTACGTTCCTCCCAAAGGTGATA130.10415832064738402No Hit
AAGAAGAACTGAGGAGTGGTGGACCAGGCTCGAACACTGGCCGAGGAGGAGCTCTCTGCCTGCCAGAAGAG130.10415832064738402No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC130.10415832064738402No Hit
AGTCTCTACTAAAAATACAAAAAAAATTAAAAAATCAGCTGGACGTGGTGGCTCATGCTACTGTAGAGGCT130.10415832064738402No Hit
AGATTATCTTAAAACCAGAGTTCACTGATCTCACATTTTAAAAAGAAAAAAGTTTGTTAACTGTTTTAATC130.10415832064738402No Hit
ACAAAGGTGTTTTTCTCTGTTAAGGAATTTATAAATAAAGGACTGTGTGTACCTTGGTGACAAAATAAAAA130.10415832064738402No Hit
CTACTATACAAACTACAGAGTTTATGATTTAAGGTACTTAAAGCTTCTATGGTTGACATTGTATATATAAT130.10415832064738402No Hit
ACCGAGCTCTGTGGGAAAAAAGAAAAACCTGCTCCCTTCGCTCTGCTGGAAGCTGGAGGGTGCTAGGCCCC130.10415832064738402No Hit
ATCTACAGCAAGTGATAAGGAAACTGGGCAAAAAAGGAAAAAAGCATACATAGGAAAATGAAAGATTCTCT130.10415832064738402No Hit
TGCTTGGATTGTTCATGAAAATTTCATAAGACATTAAACAAAGCTAGCCATCATCTCAAGTTATTTCCCTG130.10415832064738402No Hit
TGCTAGTCCTAGATTACTTATTGATTTAAAAACAAAAAAACACAAAAAAATAGTAAAATATAAAAACAAAT130.10415832064738402No Hit
GTTGTTGACTCGCATAATGCGAGAGATGTTGCGGAAACGAGCCAAGGAGCCGTAGGATCCCAGCATGTCTG130.10415832064738402No Hit
TGGTAAGAGCCCATTAGCTGCCAAGAGGGAATAAGGAATAAATTTCAAAAAATGTACAATTTCCTTTAGAG130.10415832064738402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)