FastQCFastQC Report
Tue 7 May 2024
EGAF00002877935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7642
Total Bases542.5 kbp
Sequences flagged as poor quality0
Sequence length71
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA3955.168803978016226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2012.630201517927244No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG610.7982203611619995No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA530.6935357236325569No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440.575765506411934No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC270.3533106516618686No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC260.3402250719706883No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC220.287882753205967No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC170.22245485475006543No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG160.20936927505888508No Hit
GAGTAAAGAAGATGATGATGTAATAGTGAATAAACCTCATGTTTCGGATG160.20936927505888508No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT150.1962836953677048No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC150.1962836953677048No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC140.18319811567652447No Hit
GAACATGGTCAATGAAACAGTGATTTGGAAAATAGTATTAAGGAATTCAC140.18319811567652447No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG140.18319811567652447No Hit
TCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.17011253598534415No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA120.15702695629416383No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG120.15702695629416383No Hit
TCCCTACACGACGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1439413766029835No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATC110.1439413766029835No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT110.1439413766029835No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTG110.1439413766029835No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA110.1439413766029835No Hit
TCCCTACACGACGCGGGGGACTATGAGAATCGAACCCATCCCTGAGAATC110.1439413766029835No Hit
CCTCTAGGGAATGGGATTTAGATTTAAAAAGGGTGGGATGGGAAACTGTG100.13085579691180318No Hit
GTCTGCAAGACGACAGCTAAGGAAGAAATACCCAGACAAAATCTTTGGGA100.13085579691180318No Hit
ACATAGTATGAGGATTTACTTTATATATAAGTAACAAAGAGTAGTGTGAA90.11777021722062288No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG90.11777021722062288No Hit
TCTTTTCAGTGTTCACATCTTGTTCCCCATTTCTCACTTGTGTCACCAGC90.11777021722062288No Hit
TTTCAGAAGCTTTCCGCCCGGCTAAGAGCCTTGAGGCAGAGGCAGCTGGA90.11777021722062288No Hit
GAATAAAGAGAATCTGGACCACTACCCGGGCACCAGGGACCACAGCACTG90.11777021722062288No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATA90.11777021722062288No Hit
GGCCTTAACCAATAGGTTGGAAGACAAGACCAATTGAAGAGTTAGGAAAT90.11777021722062288No Hit
GTCCACATGGGACACTGCTGCCTTCATTGTTTTCCTCACCTTCATGGGCA90.11777021722062288No Hit
ATACTCCTCAATAGCCATCGCTGTAGTATATCCAAAGACAACCATCATTC90.11777021722062288No Hit
GGGTACAAACCTGAAAAGGGAGAATCAAAGATTGATGGAAATATGTGAAA90.11777021722062288No Hit
AACCCCATCTCTAATAAAAATACAAAAAATTAGCTGGGCGTGGTGGCAGG90.11777021722062288No Hit
AGATTTAATTATAAGGTAGTACCTAATTTGTTCAATGTATAACATTCTCA90.11777021722062288No Hit
TTCTCCTTGTTGTTGGTTGTTTTTTCCTTTGCTCTTTCCCCCTTCCATCT80.10468463752944254No Hit
CCACTAATAGTTATGTCATCCCTCTTATTAATCATCATCCTAGCCCTAAG80.10468463752944254No Hit
CATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACGAAACGG80.10468463752944254No Hit
GAATCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC80.10468463752944254Illumina Single End PCR Primer 1 (100% over 40bp)
CTCATAACCCTCAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGC80.10468463752944254No Hit
TCCCTACACGACGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10468463752944254No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA80.10468463752944254No Hit
GAATGTTCGTCTAACAGAAGAAAATGCTGTAAATATTTGTAACAACATTT80.10468463752944254No Hit
GAGTATAACCACTAGTTGCCTAGACAAAAGCTAATTTCTACAAAATCAAA80.10468463752944254No Hit
GTTACCTGGAAATACTGATGATAACATATTACCTTATTTGAACAAGTTTT80.10468463752944254No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAAT80.10468463752944254No Hit
TTTTCCTCATTGAGCAAGCTGGGATTCTCTAATGTAGAAATCAAGCCATC80.10468463752944254No Hit
GACTTGCACAGTCCTTCTGAGATTGTTAAGCAGATTACTTCCATCAGTAT80.10468463752944254No Hit
GGATATGGAATATTATCTTGTAAAATGGAAAGGATGGCCAGATTCTACAA80.10468463752944254No Hit
AATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG80.10468463752944254No Hit
GTCACCATCTTAGACTCTTCTGAGGAAGATAAGGAAAAAGAAGAGGTTGC80.10468463752944254No Hit
ACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCAC80.10468463752944254No Hit
ACAAAGAAACATGTCAGCAGGGCCTATGGTGGTTCCATGTGTGCTAAATG80.10468463752944254No Hit
GTTTTTAGACCTGTGTTACTAAAAAAAAGATGAATGTCCTGAAAAGGGTG80.10468463752944254No Hit
GATATCTTCTAGACATATTGAACACATTTAAGATTTGAGGGATATAAGGG80.10468463752944254No Hit
GGCTTTAGTAGGTATAGAAGACAATGGATTACCATCTTTATTGCTGTAAT80.10468463752944254No Hit
GTATAAAATATACCCCAGATTTTGTAGACTTAGTAAGAAAAGAAAGACTG80.10468463752944254No Hit
GGTCTGAATATTTGATAGAACCAATTGTTGTACATAAAACAGATCTGCGC80.10468463752944254No Hit
ATACAGGACTTGTTGCCAATTGGGTAATTTTCATTAGTTGTTTTGTTTGT80.10468463752944254No Hit
CCCTCAGCACTTGCTCAGTAGTTTTGTGAAAGTCTCAAGTAAAAGAGACA80.10468463752944254No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT80.10468463752944254No Hit
AGCCTGCTCCACCCAGAGAAGCACACTTTGTGAGAACCAATGGGAAGGAG80.10468463752944254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)