FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7642
Sequences flagged as poor quality0
Sequence length71
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG2222.904998691442031No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1892.4731745616330802No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA740.9683328971473436No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT550.7197068830149176No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA500.654278984559016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380.4972520282648521No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC250.327139492279508No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC200.26171159382360637No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGC190.24862601413242605No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG150.1962836953677048No Hit
GAACATGGTCAATGAAACAGTGATTTGGAAAATAGTATTAAGGAATTCACCCAGAATGTAGTAAGAGAGAA140.18319811567652447No Hit
GAGTAAAGAAGATGATGATGTAATAGTGAATAAACCTCATGTTTCGGATGAAGAGGAAGAAGAACCTCCTT130.17011253598534415No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA130.17011253598534415No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC120.15702695629416383No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA120.15702695629416383No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC110.1439413766029835No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT110.1439413766029835No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA110.1439413766029835No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCA100.13085579691180318No Hit
CCTCTAGGGAATGGGATTTAGATTTAAAAAGGGTGGGATGGGAAACTGTGTTTTTCATTTTAAGTCCTTCT100.13085579691180318No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTA100.13085579691180318No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG100.13085579691180318No Hit
GGCCTTAACCAATAGGTTGGAAGACAAGACCAATTGAAGAGTTAGGAAATGTGAGTTTTTGTTACTTCTGT90.11777021722062288No Hit
AACCCCATCTCTAATAAAAATACAAAAAATTAGCTGGGCGTGGTGGCAGGCGCCTGTAATCCCAGCTACTT90.11777021722062288No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGAGCCCGTATTTACCCTATAA90.11777021722062288No Hit
AGATTTAATTATAAGGTAGTACCTAATTTGTTCAATGTATAACATTCTCAGGATTTGTAACACTTAAATGA90.11777021722062288No Hit
GGGTACAAACCTGAAAAGGGAGAATCAAAGATTGATGGAAATATGTGAAAGTCTGGAGAAAACTAAGCAGA90.11777021722062288No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC90.11777021722062288No Hit
TCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11777021722062288No Hit
GTCTGCAAGACGACAGCTAAGGAAGAAATACCCAGACAAAATCTTTGGGACGAATGAAAATTTGTAACTCT90.11777021722062288No Hit
ATACTCCTCAATAGCCATCGCTGTAGTATATCCAAAGACAACCATCATTCCCCCTAAATAAATTAAAAAAA90.11777021722062288No Hit
GTATAAAATATACCCCAGATTTTGTAGACTTAGTAAGAAAAGAAAGACTGTAAAATATCTCAATACCTGTT80.10468463752944254No Hit
GTCCACATGGGACACTGCTGCCTTCATTGTTTTCCTCACCTTCATGGGCACCGTGCTGCTCCTGCTGCTGC80.10468463752944254No Hit
GACTTGCACAGTCCTTCTGAGATTGTTAAGCAGATTACTTCCATCAGTATTGAGCCAGGAGTTGAGGTGGA80.10468463752944254No Hit
GGATATGGAATATTATCTTGTAAAATGGAAAGGATGGCCAGATTCTACAAATACTTGGGAACCTTTGCAAA80.10468463752944254No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC80.10468463752944254No Hit
TCCCTACACGACGCGGGGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTA80.10468463752944254No Hit
ACATAGTATGAGGATTTACTTTATATATAAGTAACAAAGAGTAGTGTGAACAGGAGGGAATTAATCTACAT80.10468463752944254No Hit
ATACAGGACTTGTTGCCAATTGGGTAATTTTCATTAGTTGTTTTGTTTGTTTTGATTTGAAACCTGGAAAT80.10468463752944254No Hit
CCACTAATAGTTATGTCATCCCTCTTATTAATCATCATCCTAGCCCTAAGTCTGGCCTATGAGTGACTACA80.10468463752944254No Hit
GTTTTTAGACCTGTGTTACTAAAAAAAAGATGAATGTCCTGAAAAGGGTGTTGGGAGGGTGGTTCAACAAA80.10468463752944254No Hit
TTTCAGAAGCTTTCCGCCCGGCTAAGAGCCTTGAGGCAGAGGCAGCTGGACCGAGCTGCGGCTGCTGTGGA80.10468463752944254No Hit
ACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTA80.10468463752944254No Hit
GTTACCTGGAAATACTGATGATAACATATTACCTTATTTGAACAAGTTTTCCTTTATTGAGTACCAAGCCA80.10468463752944254No Hit
AGCCTGCTCCACCCAGAGAAGCACACTTTGTGAGAACCAATGGGAAGGAGCCTGAGCTGCTGGAACCTATT80.10468463752944254No Hit
GGTCTGAATATTTGATAGAACCAATTGTTGTACATAAAACAGATCTGCGCATATATATATATGTATAAAAA80.10468463752944254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)