FastQCFastQC Report
Wed 8 May 2024
EGAF00002877957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437420
Total Bases102 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106230.7390324331093209No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA55140.3836039570897859No Hit
GTACTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG52810.3673943593382588Illumina Single End PCR Primer 1 (100% over 41bp)
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC23230.16160899389183397No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT22290.1550694995199733No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA18440.12828540023096938No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT18100.12592005120284955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16040.11158881885600591No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC15920.11075398978725773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)