FastQCFastQC Report
Tue 7 May 2024
EGAF00002877979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85857
Total Bases6 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8601.001665560175641No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA2770.3226294885682006No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC1410.16422656277298298No Hit
CCATCCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG1310.15257928881745228Illumina Single End PCR Primer 1 (100% over 41bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.12928474090639086No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1090.12695528611528473No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1040.12113164913751936No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT900.10482546559977637No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG880.10249601080867023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)