FastQCFastQC Report
Wed 8 May 2024
EGAF00002878010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545387
Total Bases38.7 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51250.9396996994794522No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA37270.6833679570653499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15240.2794346033183776No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC10620.1947241133360348No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG7080.12981607555735653No Hit
GAGCTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG6800.12468210646751755Illumina Single End PCR Primer 1 (100% over 41bp)
GATCTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG6710.12303190211721218Illumina Single End PCR Primer 1 (100% over 41bp)
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT6440.11808128906629606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)