FastQCFastQC Report
Wed 8 May 2024
EGAF00002878035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620475
Total Bases44 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31590.5091260727668319No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA25850.41661630202667316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15570.2509367823038801No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT6710.1081429549941577No Hit
GATATAACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG6610.106531286514364Illumina Single End PCR Primer 1 (100% over 41bp)
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA6450.10395261694669407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)