FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4324
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA771.7807585568917668No Hit
GTGGAATAGCTCAGAATGTCAGTTCTGTTTTAAGTAACAGAATTGATAACTGAGCAAGGAAACGTAATTTG150.3469010175763182No Hit
CTCTTAGAGAGAAAATAGAGACTAGTACAACCAAGAAACTAAAGCAGATTACTACCAAAAAATTTGAATGT140.32377428307123035No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCC140.32377428307123035No Hit
GTCTGAAACCACTTCTGAACACACTTGTCAGCTCTGTGGTGGTTGGTTTGATACTAAAATTGGATTATCAA130.30064754856614245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.30064754856614245No Hit
TCCCTACACGACGCGGGATGGTAACTCACACCTGTAATCTCAACGCTTTGGGAGGCCAAGGCGGGAGGATT130.30064754856614245No Hit
GTATTTAGACCCAGTAAATGCAGAAATAGAAACAAACGGTCAGAAGACATATCGTGAGAGAGAGCGAGAGA130.30064754856614245No Hit
CAGTCATCCCAGCAATCGCTTGGTTCAGTTTCATTCAGCTCTCTATGGACCAGTAATCTGATAAATAACCG120.27752081406105455No Hit
GATTAAGAAAGATCTCTTGCAAAATAAATATGTGATTGAGGGATAAGGGAAGGAACCCCTAGGAGTATTAA120.27752081406105455No Hit
GCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGA120.27752081406105455No Hit
GAAGTATATGTAAATGTCTGTTTTTCATAATTGCTCTTTATATTGTGTGTTATCTGACAAGATAGTTATTT110.2543940795559667No Hit
CTCATATTAAATATAGAGTTGTTGAACCAGGTTGTTTTACTGTCTGAACATACTACCTCACTTTTAGACTA110.2543940795559667No Hit
GTATTTAAACCTCTCTATTCCCTGCCATAACATCTTTTGCCACGTATAGCTGGAATTAAGTGTTGTCTTGG110.2543940795559667No Hit
GTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTTG110.2543940795559667No Hit
GGCAAAAACATTGAACTTGCTGTCATGAGGCGAGATCAATCCCTCAAGATTTTAAATCCTGAAGAAATTGA100.23126734505087881No Hit
TCTGTAATCCTAGTGCTTTGAGAGTCTGAAGCAGGAGGATTGCTTGAGGCCAGGAGTTTAAGGCTGCAGTG100.23126734505087881No Hit
ACATGACAATTTGGCTATGAAAATTTGCAATGAGATCTTAACAAGTCCGTGCTCGCCAGAAATTCGAGTCT100.23126734505087881No Hit
GTAAAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTTT100.23126734505087881No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGA90.20814061054579094No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGA90.20814061054579094No Hit
GTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCA90.20814061054579094No Hit
GTAATCTGAGCACTTTGGGAGGCCGAGAGACCAGCCTGACCAATATGGTGAAAATCCGTCTCTATTAAAAA90.20814061054579094No Hit
CTTGTCAGCTCTGTGGTGGTTGGTTTGATACTAAAATTGGATTATCAAATCATGTTAGAGGCCACTTGAAA90.20814061054579094No Hit
TTCACAAACCAGGTGGAGACACAGAATTGATTGGAGACGTCTGTGGACCAGGCTGGGCAGCTAACATCTCT90.20814061054579094No Hit
GAGTATAATTGATACTCTTAGAGAGAAAATAGAGACTAGTACAACCAAGAAACTAAAGCAGATTACTACCA90.20814061054579094No Hit
GTGGTGCACCCTTGTAATCCCAGCTACTTGGGAGACTGAGGCAGGAGAATTGCTTGAACTTGGGAGGCGGA90.20814061054579094No Hit
GAGTAAATGATTGAGATAAGTTGAAAATTAAGCTTCCAGCCATACAAAAGTGGAGATTGCTATAGTGTCAA90.20814061054579094No Hit
GCCCATTGTAGCATATGTTTGATTTTTTTTTATTATTTCATAAGAAAACTACGTTAATTTTACCTTACTTT80.18501387604070307No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATT80.18501387604070307No Hit
GTACATATGTAGATATTGTTTTCTGTAAATAACCTATTTTTTTCTCTATTCTCTGCAATTTGTTTAAAGAA80.18501387604070307No Hit
AAATAGCACTAAACGTTTTCTTTCTGCAACCTGTACTTACAGATTCTTCCTGTAAACTAAATAAAAAAAAA80.18501387604070307No Hit
TCCCTACACGACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.18501387604070307No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACG80.18501387604070307No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT80.18501387604070307No Hit
CCCTTTATTTCATCTGTATTTAAACCTCTCTATTCCCTGCCATAACATCTTTTGCCACGTATAGCTGGAAT80.18501387604070307No Hit
TTCCCTACACGACGCGGGAGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGC80.18501387604070307No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGC80.18501387604070307No Hit
CAACAAGAGCTGTATTTTAAGTATTTAGACAGTTCTTTGTTAGCTGGTTTCTAGTTGGTTATCTAGTTACC80.18501387604070307No Hit
CTTGAGGACTTGGTCTTCCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCCGGCT80.18501387604070307No Hit
GATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGAC80.18501387604070307No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAA70.16188714153561518No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGT70.16188714153561518No Hit
ACATAATGCTAGTCCTAGATTACTTATTGATTTAAAAACAAAAAAAACACAAAAAAATAGTAAAATATAAA70.16188714153561518No Hit
TCCCTACACGACGCGGGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16188714153561518No Hit
GTGTGGTGGGAACCAAGAAGCGGGTGCTCACCCTCCGCAAGTCCTTGCTGGTGCAGACGAAGCGGCGGGCT70.16188714153561518No Hit
TTCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16188714153561518No Hit
GAACTGGACTGGAAGAACCAGCCATCTTTGCTGGGATACCAGCCCAGAGGTTGTAGAAAAAATCCAGGCAA70.16188714153561518No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16188714153561518No Hit
GTCTGAAGCAGGAGGATTGCTTGAGGCCAGGAGTTTAAGGCTGCAGTGAGCTGATTGCATCACTGTACCCC70.16188714153561518No Hit
GTATAGCTGGAATTAAGTGTTGTCTTGGAGCTGTTGTACATTTAAGAATAAACTTTTGTAAAAAAAAAAAA70.16188714153561518No Hit
CTCTATTCCCTGCCATAACATCTTTTGCCACGTATAGCTGGAATTAAGTGTTGTCTTGGAGCTGTTGTACA70.16188714153561518No Hit
CGCGGGATGAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16188714153561518No Hit
ATATAGAGTTGTTGAACCAGGTTGTTTTACTGTCTGAACATACTACCTCACTTTTAGACTAGGATACTACC70.16188714153561518No Hit
CATCCCAGCAATCGCTTGGTTCAGTTTCATTCAGCTCTCTATGGACCAGTAATCTGATAAATAACCGAGCT70.16188714153561518No Hit
ATGCAATTCCCGGACGTCTAAACCAAACCACTTTCGCCGCTACACGACCGGGGGTATACTACGGTCAATGC70.16188714153561518No Hit
CTAATAAGACATCGGCTATCTAAGCGTGTGGGTGCCCCCTTTCTGCCAGCAGTTCTGGTTCTTAAGAAAAT70.16188714153561518No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAA70.16188714153561518No Hit
ACAAAATGCAGCATTTCAAAAGAACTGGCACAGGAACACCTGTTAAACGAGTTAGAAAAGCTATAGAGAAG70.16188714153561518No Hit
CCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAAAA70.16188714153561518No Hit
GTAATCCTAGTGCTTTGAGAGTCTGAAGCAGGAGGATTGCTTGAGGCCAGGAGTTTAAGGCTGCAGTGAGC70.16188714153561518No Hit
AGGTGGAATGAAGAAAAAGACCACCCATTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAGGACCAGATCA70.16188714153561518No Hit
ACTTTCATTGTAAATAAGCCTGTCTTCCTATCTGGATTTTTTGTGTGCATACATATTCTACTGATTAACTA60.13876040703052728No Hit
GAAGAAAGCATTCATGGGACCACTGAAGAAAGACCGAATTGCAAAGGAAGAAGGAGCTTAATGCCAGGAAC60.13876040703052728No Hit
GTTCAGAAACTGAACCCCACCCTCCTGGGAAACGCCCATTGGAGTGTTGTTTTTAACCTTTGTACAGTATT60.13876040703052728No Hit
CTTATGTACCGAGCAAATGCCAGGTCTAGCAAACATAATGCTAGTCCTAGATTACTTATTGATTTAAAAAC60.13876040703052728No Hit
CAAGAATGTTAAGCAACTTTGTTTTTGCGGTGCTCCCTATTTCATAGTATCTTCAAATCAAAAATAAAAAT60.13876040703052728No Hit
GACCTGGTGTCCGAGTACCAGCAGTACCAGGATGCCACAGCCGAGGAGGAGGGCGAGTTCGAGGAGGAGGC60.13876040703052728No Hit
GGAAAAATGTTAACAAATGTGGCAATTATTTTGGATCTATCACCTGTCATCATAACTGGCTTCTGCTTGTC60.13876040703052728No Hit
TCCTAATAGGAATTAACCTCCAAATAAAACATGACTGGTACGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13876040703052728No Hit
TTCCCTACACGACGCGGGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13876040703052728No Hit
CAATGAAGCCCTCAAGGGCTGAAAATAAATAGGGAAGATGGAGACACCCTCTGGGGGTCCTCTCTGAGTCA60.13876040703052728No Hit
GGACTACTCAGGAGGCTGAGGCAGAGAATCGCTTGAACCTGGGAGACAGAGGCTTCAGTGAGCTGACATCC60.13876040703052728No Hit
AGTTTAAAGCCGTCCTAGTGGAGCAAGCCCTAAAGCAGATTTAATTTTTGCCATTTTCCAAGAATGACGGT60.13876040703052728No Hit
TCCCTGGACTGGCCATGAAGTGAGGAGATGGTTATTTAAAGAGAATTCCCTATTTATTTGACAAAAAATCC60.13876040703052728No Hit
TGGCAAAACTGGAAGAGTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGG60.13876040703052728No Hit
TTCCCTACACGACGCGGGATGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGCGCCATTGTACTCCAGGGCA60.13876040703052728No Hit
GGACTGGAAGAACCAGCCATCTTTGCTGGGATACCAGCCCAGAGGTTGTAGAAAAAATCCAGGCAAGAGGT60.13876040703052728No Hit
TTCCCTACACGACGCGGGGAATCAGTCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13876040703052728No Hit
CGCCGATGGCTGCTGGGGACAGCGCCCCTGAGCTGCAACAAGGTGGAAACAAGGGCTGGAGCTGCGTTTGT60.13876040703052728No Hit
CTGTAGCTCTAGCTACTCGGGAGGCTGAGGCAGGAGAACGGCGTGAACGCGGGAGGCGGAGCTTGCAGTGA60.13876040703052728No Hit
AGCCTGGCCAACATGATGAAACCCTGTCTCTACTAAATATACAAAAATTAGTTGGGTGTTATGGTGCATGC60.13876040703052728No Hit
CCTTGAACCCTATCAATAACATTTTTCACATATCTCAATTTTTGTGTGTCTTTGTAGTTGTATGTGGGCCA60.13876040703052728No Hit
TTCTACACTCTGTCCTACTCCCACAGAATTTTCAAGCCCTTAAGAGTTTAGTTAAAATAAAATTTTTGAAA60.13876040703052728No Hit
CCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTG60.13876040703052728No Hit
ATGTAAATGTAAACTCAGCATCAAAATATTTCAGTGAATTTGCACTGTTTAATCATAGTTACTGTGTAAAC60.13876040703052728No Hit
GGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13876040703052728No Hit
GTTCAGTTTCATTCAGCTCTCTATGGACCAGTAATCTGATAAATAACCGAGCTCTTCTTTGGGGATCAATA60.13876040703052728No Hit
ACCATGCCTGGAAGGCCAATGCCATAGGCCGGGGTGCCAAGTCAGTGCGTGAGTTCCTGGAGAAGAACTAT60.13876040703052728No Hit
CTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTAGA50.11563367252543941No Hit
AACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACC50.11563367252543941No Hit
GTTAACAAATGTGGCAATTATTTTGGATCTATCACCTGTCATCATAACTGGCTTCTGCTTGTCATCCACAC50.11563367252543941No Hit
GAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAG50.11563367252543941No Hit
TCCCTACACGACGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
CTGTCCTACTCCCACAGAATTTTCAAGCCCTTAAGAGTTTAGTTAAAATAAAATTTTTGAAATTAGAAAAA50.11563367252543941No Hit
CAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTG50.11563367252543941No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAG50.11563367252543941No Hit
AAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTA50.11563367252543941No Hit
GACGACATGATTGTTTATCTAGAAAACCCCATCGTCTCAGCCCAAAATCTCCTTAAGCTGATAAGCAACTT50.11563367252543941No Hit
CAATAACATTTTTCACATATCTCAATTTTTGTGTGTCTTTGTAGTTGTATGTGGGCCACTTACTAATATTT50.11563367252543941No Hit
GATGTTGTACCTTGTGGTATATCTGTCCCTATTAAATAAGTGTTGTTTTCTCCTCTTTAATATTGCTGTGA50.11563367252543941No Hit
GAGTGACTTCAGTCATCCCAGCAATCGCTTGGTTCAGTTTCATTCAGCTCTCTATGGACCAGTAATCTGAT50.11563367252543941No Hit
GCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACA50.11563367252543941No Hit
CCCAATAACCAGGTGCTTGGCAAAATCGAGCGGGCCATTGGCCTCAAGCTCCGGGGAAAGGACATTGGAAA50.11563367252543941No Hit
AAACAGAATAAGCAAGTCAGAGGAGAGAGAGATAATGTAGAAAACTGAAGAAATTTTCAAGGAAAAAATTA50.11563367252543941No Hit
TCCCTACACGACGCGGGAGCCGGGCGTGGTGGCGGGCGCCTGTAGCTCTAGCTACTCGGGAGGCTGAGGCA50.11563367252543941No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA50.11563367252543941No Hit
TCCCTACACGACGCGGGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
TCCCTACACGACGCGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
CCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
AAATACGGCATCATCTGCATGGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAAAGAGGC50.11563367252543941No Hit
AGACAGTTCTTTGTTAGCTGGTTTCTAGTTGGTTATCTAGTTACCAATGCTGCAGTCCTGCAGTCACCTAT50.11563367252543941No Hit
ATCCCAGCCCATGTACTAATAAAAGAAAGTCTTTGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
ATTGTAAGGTATTTTACACGTGTCCTGATTTTGCCACAACCTGGATATTGAAGCTATCCAAGCTTTTGAAA50.11563367252543941No Hit
ATGGTCACCATGTGACCAAACATGGCACATGTTTACCTGTGTAATGAATCTGCACATCCTGCACATGTACC50.11563367252543941No Hit
TCCCTACACGACGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
TTCCCTACACGACGCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
CTTTGGGAGGCCGAGGAGGGCGGATCACAAGGTCAGGAGATCAAGACCATCCTGGCTAATATGGTGAAACC50.11563367252543941No Hit
AGACCAAGTAGAGGCCTTGGTGCTGGTTGGGGTGGGGCCTGCAGAGTCTTAGTTACTGATTTCATTTTCAA50.11563367252543941No Hit
CTACACGACGCTGGATGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11563367252543941No Hit
CCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATT50.11563367252543941No Hit
TCTTTTAACTGGTGAACAGTTTATTCAGTTGCGAAGGGAATTAGCTTCTGTAAATGGTCATAGTGGTGATG50.11563367252543941No Hit
TTCAAATGCTGTAATTAACAACACTTAAAAAACTTGAATAAAATATTGAAACCTCAAAAAAAAAAAAAAAA50.11563367252543941No Hit
GTGCTAGCTCCTCCCACCTCAGCACCTGCTGCATCTGGAGCAGCCCAAGCCTCAGGATGGACAAGAGGAAA50.11563367252543941No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTA50.11563367252543941No Hit

[FAIL]Adapter Content

Adapter graph