FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25320.031670625494854No Hit
ATAACAATCAGTGTGGATTTCCACTCTTTTCAGTCCTTCATGTTAAAGATTTAGACACCACATACAACTGG120.9501187648456058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.791765637371338No Hit
ACGTTTTTGAAGGGGGTGAGATTTATTTGTGGTGCCCTGTTCCAAAGGGGAGAAGATTAAGAGATAGGAGG100.791765637371338No Hit
GATATTTTAAAAGTTTTTTATTCTTCGGGCCTGGAAAGTGTACAAAGTCAGTGTAGGAGAAAAAAATCCAT80.6334125098970704No Hit
GTAGCTGCCACATTGATTTTTCTATAATCACTTGGGGTTTGTACATTTTTGGGGGGAGAGACACAGATTTT70.5542359461599367No Hit
GGCACTGTTCCTGGTGAACCTTGGACCACAGCATGTCAGTGCTCTAGGGATTGTCTACTCCAGGGATTTTC70.5542359461599367No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG60.4750593824228029No Hit
CTCCGTTTTCTAGCAAGTATTAAGGGAGCTGTGTCTGAAATGGCACTGTCTTGTCAGTCATTTCTGTTTAC60.4750593824228029No Hit
CAGTAAAATCCACTGTGTGTGGAAGGCAGAAGCTAGCGGTTGTATCCCAAGCATCTTTTGTATTTGTCTTT60.4750593824228029No Hit
ACACTGTGAAACCCTGTCTCTACTAAAATACAAAAAATTAGCCAGGTGTGGTGGTGGGTGTCTGTAACCTC60.4750593824228029No Hit
GTTCTAGTGTTTGATAGCCCAGTAGGATGACTATAGTTAACAGTAATTTATTGTATATTTCAAAATAGCTG60.4750593824228029No Hit
CCACAGCTGGCTGGGGCATGGGCATTGATCGAGTCGCCATGTTTCTCACGGACTCCAACAACATCAAGGAA60.4750593824228029No Hit
GTGCTGTCTTCACATACTGGATTTTGCCAATCTTCAGGAGATGAAGCCTTATCTAAATCAGTGCCTGTCAC50.395882818685669No Hit
TCTCTGTTTCACGTAGTTGGGTTGACAAGTTTCTGCCTTTAAGATAAATGAGTAATAGTCTAATGACCAGC50.395882818685669No Hit
GTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTG50.395882818685669No Hit
GGCTACCACTTCGAAGACACCGCGGGAGCTTACTTGCTCAACCGCACTCTATGGGATCGGTGCGAGGCGCC40.3167062549485352No Hit
GAAGTAAGACAGCTTGCAGAGGATTTCCTTCGTGATTACACCCAGATCAACGTAGGCAATCTGGAGTTGAG40.3167062549485352No Hit
CTCAATAAACCCTATTTCTTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3167062549485352No Hit
GGCACAAGAAGGGGAATAAAGGGGCTGTGTGAAGGCACTGCTGGGAGCCATTAGAACACAGATACAAGAGA40.3167062549485352No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTA40.3167062549485352No Hit
CTACTGACTGCCTTGATGTTTATTGGCTAGAAGTCATTAAGTGTGCCTGCCATCTTATGATTTGGACTGGT40.3167062549485352No Hit
CGACGCGGGGTAATTAAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3167062549485352No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3167062549485352No Hit
CCATGAACTCTGTAAAAGTTATAGAAGACTGCAGTGAAAATCACAGTTACAAAAGCGACAGTCTTCTATTT40.3167062549485352No Hit
GGCTCAAAACACGACAGGTGTGTCCATTGGACAACAGAGAGTGGGAATTCCAAAAGTATGGGCACTAGGAA40.3167062549485352No Hit
GATTCATGAGATCTATACTGTTGGAAAACGCTTCAAAGAGGCAAATAACTTCCTGTGGCCCTTCAAATTGT40.3167062549485352No Hit
GCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAGTGGTTCGAGACCATCCTGGCCAACATGGGG40.3167062549485352No Hit
GTGCTGCTGATGTGCAACAAATCTTACTGTGCCGAGATCGCTCACAATGTTTCCTCCAAGAACCGCAAAGC30.23752969121140144No Hit
GTCTCAAAAAACAAACAAACAAACAAAAGGTAATTGTGCAGTGAGGAATATGTTAATATACTGACTACAGT30.23752969121140144No Hit
GGATTTGGAGTTTGTATCCCCTGGTCCCTCTGGGATGCTCATTAAAACCTTCCCACTCCTTGGAAAAAAAA30.23752969121140144No Hit
TGGTTATCACTAGGCTAAAACTGGACAAAGACCGCAAAAAGATCCTCGAACGGAAAGCCAAATCTCGCCAA30.23752969121140144No Hit
TTAGAACACATTGTATTGGTATTACAACCAGATTATATTCTTGACGTTGACTTCATTAAAATTATCTACAA30.23752969121140144No Hit
GTTCAAATGATTTTAATTATTGGAATGCACAATTTTTTTAATATGCAAATAAAAAGTTTAAAAACAAAAAA30.23752969121140144No Hit
CATTCATTAATATATGGAATTCCTCTACTGCCCAGCCCCTCCTGATTTCTTTGGCCCCTGGACTATGGTGC30.23752969121140144No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT30.23752969121140144No Hit
CTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTA30.23752969121140144No Hit
TTCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.23752969121140144No Hit
GTGCAGACATTCAATTGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.23752969121140144No Hit
GATTGAGGATGTCACCCCCATCCCCTCTGACAGCACTCGCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGT30.23752969121140144No Hit
GTTCTTTGTGTATTTTTTCTACAGAGGATTTTCTCTGATTTTATTTTCTTTGTTTCTGACTCTAATAATTA30.23752969121140144No Hit
GGAAATAATGTACCCATGTAGACTAGCAAAATAGTATGTAGATGTGATCTCAGTTGTAAATAGAAAAATCT30.23752969121140144No Hit
ACGCGGGGGAGGAGGACTTTTTGTTTTTTAAATGTAGCTCTTTTAGAAAACGATGAAAAGGATGAAGAGGA30.23752969121140144No Hit
CAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGT30.23752969121140144No Hit
TGTAAAAAGATCTACATACAAACAATGCAACCAACTATCCAAGTGTTATACCAACTAAAACCCCCAATAAA30.23752969121140144No Hit
GTTTATCTATTGTCAGTATTTTATGTTGAATATGTAAAGAACATTAAAGTCCTAAAACATCAAAAAAAAAA30.23752969121140144No Hit
CCCATTCTTGCACGATCACCATTAAAATTCTCTCTTCCATTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAA30.23752969121140144No Hit
CATTAAATACAGATGCAGATAAAATATCTTTAGTGCTGCAACATTTTACCTAACTTTTTGTATGTTTTCAT30.23752969121140144No Hit
GAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCCGATCACCTCTGAGACCCACCTTGCTC30.23752969121140144No Hit
GGTACTCCTGAAATGGCTTCTGCAGAGATGGACCTATGCCGGGGACAGCACTGGAAGCAGGGTACAGTAGC30.23752969121140144No Hit
GGATCTAGAAGAATATGTCAAACTATAAACTGCTTGTGATTGTGAATGACTTTGTTCTTTGCTTGTGTTTT30.23752969121140144No Hit
ATATATATGTAAAAGAAAAATAAATGTTATTTGTTAGAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
ACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
GCTCACAGATCAGGTGATGCAGAATCCTCGAGTTCTGGCAGCTTTACAGGAGCGACTTGACAATGTCCCTC20.1583531274742676No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA20.1583531274742676No Hit
CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTA20.1583531274742676No Hit
GAATAATGCAAATGCAACGTTGGTGACCTTACATTACCTGGGAATCAGAAGGTTATTAGTATTCCCTAGGG20.1583531274742676No Hit
GGTCACCCACCCCCTGGTCCACATCACTGAGGAAGTAGAAGAAAACAGGACACAAGATGGCAAGCCTGAGA20.1583531274742676No Hit
CGCGGGGAGCTATGAAATAAAACATTTTAAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
CCTTGGTAACTAGCATCCAGATCAAGAAACAGAATTTTACGAGAACCTGGCTGGGCACAGTGGGTCATGCC20.1583531274742676No Hit
CCTATGCAATATTTTTTACTTTTCTAATAAACATGTTTGTTAAAACAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
TGTAATAGTGAAACCTGTACTCAAAATATAAGCAGCTTGAAACTGGCTTTACCAATCTTGAAATTTGACCA20.1583531274742676No Hit
AACTTTAGTCGTGTAATAAACTCACCAGTGGCAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
GTTCACACCACTGCACTCCAGCCTGGCAACAGAGTACGTGAAAATCTGTCTCAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
CCTCTAGTCCCAGCTACCTGGGAGGCTGAGGTGGGAGAATCGCTTGGACCTGGGAGGCAGAAGTTGCAGTG20.1583531274742676No Hit
GCTTTCCTATGTTTACCCAGGGGACCTCCTTTCAGATGAACTGGGAAGAGATGAAATGTTTTTTCATATTT20.1583531274742676No Hit
ATATTCCTTTCTAACTTCAAACGTCTGCATTCATGTATAATAGCCATTTTATGCACAGATGAGGTGATTCC20.1583531274742676No Hit
GTGCTGCTGATCTGGGCCTGAATAAGGGTTATCGAATGGTGGTGAATGAAGGTTCAGATGGTGGACAGTCT20.1583531274742676No Hit
GGTTTGTCTCCCCTCCACCGCCAGACTGAAGGCTGAGGCCCATGGCAACTACTGAGCCATGGAGTTGCATG20.1583531274742676No Hit
GGTTTATCTTCAGTTGTCATTCATGCCAGGTTAATTTAATTAACTTAGTAAAACATACATAACAACAAATA20.1583531274742676No Hit
GGCGCGCGCACACCCGCTGATCTATCACTCTCGTTCTTGTAGCTCCCAGCCGAGGACGTCGGATGTCATCA20.1583531274742676No Hit
GTCCAACAATAAACAGGAATTTTATTTTGCTGAGTTGTTCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
ATTTCACATCTCCAAGGGACCCTTCCTTTCATTTTACACTTTGTTACTAATTTGCAGAACTCTATTAATTA20.1583531274742676No Hit
AGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGCCCTCACGGAGGCGGGGGTTCCAGGGCACGAGT20.1583531274742676No Hit
GAAGCAGGGTACCCTTTGGGGAGCAGGAGGCCAATAAAAAGTTCAGAGTTCTAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
GGGAAGATGGAGACACCCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAATAAATT20.1583531274742676No Hit
GCTTATGCCACTTCCTTGTTCAAAATAAAGTAACTGCCTTAATCTTAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
GATTGTGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCGTCTCAAAAAAAAAAAAAGTTGGAGGT20.1583531274742676No Hit
TCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
GACGGGCATCGCCTGATTATGCGCGGCGCTCATCTCACCGCTCTGGAAATGCTCACCGCCTTCGCCTCCCA20.1583531274742676No Hit
CCTTCACTGAACATGCCTAGTCCAACATTTTTTCCCAGTGAGTCACATCCTGGGATCCAGTGTATAAATCC20.1583531274742676No Hit
GGCAAGGAGATGGAATCCAAGTGAATAGTCAGTTTCAGCAAGAATTTCCCAGCCTGCAGGCAGCTGGGGAT20.1583531274742676No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
CCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTC20.1583531274742676No Hit
GTGCAGACATTCAATTGTTGTAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
GGCCGATCGCGACGGCTGGTTTGCATCGCCAGCTTCTATATATTACGGCCTTTTTTTTTTGCTGGATATTA20.1583531274742676No Hit
GTAGTTTTACAATCATGCTATTCTTACACTTGCTATTTTATACTTCAAATAGCCACATATTTTCAAGATAT20.1583531274742676No Hit
GGTTGCGGTGAACCGAGATCACGCCATTGCACTCCAGCTTAGGAAACAAGAGTGAAACTCCGTCTCAAAAA20.1583531274742676No Hit
GTTCCAGGCCAGCCTGGGCAACATGGAGAAACCGCGGCTCTATAAAAATGTTTTTAAATGAGCTGGGTGTG20.1583531274742676No Hit
GGTTTTAAGTTATAAATGTTAACTGCCTCTGGGTGAAAAAGTTTTTAATAAACACCTATTACCTCTTGAAA20.1583531274742676No Hit
CCTGTGCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1583531274742676No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGCTGCGCCGCCGGACCTACAGAGCTCATGGTCGGA20.1583531274742676No Hit
GACTGGCGGTTCTGGTTTTTACTCTGTGAACTTTATTTAAGGACATTCTTTTTTATTGGCGGCTCCATGGC20.1583531274742676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)