FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366
Sequences flagged as poor quality0
Sequence length71
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80133.85460693153001No Hit
GAACATGTCCTTCGAGCTACAAACCAGATCAGTCCTGCCCAGGTGCAGGGCTACTTCATGCTGTATAAAAA391.6483516483516485No Hit
GGGCCTTCTATGCTGAGCTTTATCATATCATCAGCAGCAACCTGGAGAAAATTGTCAACCCAAAGGGTGAA341.4370245139475908No Hit
GTGCTACAAATGTTTGTCACTTAAATTTCAAGTTTCTGTTTTAATAGTTAACTGACTATAGATTGTTTTCT291.2256973795435333No Hit
AAACACAAATCATGTCAAAATCACCATGCCATATTTCGTCTATTACATTAGCAAGCAATGAAATTATGATA271.1411665257819104No Hit
GGATGATCTCAACAAGAGCTGTATTTTAAGTATTTAGACAGTTCTTTGTTAGCTGGTTTCTAGTTGGTTAT241.0143702451394758No Hit
GAAAAAGGCAAACGAGAAGAAGGAGGAACCCAAGTAGCTTTGTGGCTTCGTGTCCAACCCTCTTGCCCTTC230.9721048182586645No Hit
GGATGGTTTTGCAAGAATAAGACTTGACCAAGACCGACTGCCAGTAATAGAGCCTGTTAGTATTAATGAAG230.9721048182586645No Hit
TGTTTTGGAGTACATATATATGAAAATTATTATGACACGCACTTTTCTAATCATTGTACATTTCTCAGAGT220.9298393913778529No Hit
CACACATCAATTAAAGGAGACAAAAAGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.9298393913778529No Hit
ATTGAACTCTGAACTCTGATAATTGAGAATATGGCTGCTTTTTAAAAGTGCATGAAATATCTCCCAAATTG210.8875739644970414No Hit
GAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTG190.8030431107354183No Hit
CCTGCCCACAGCTATGCCTTGGCCTCTCCAGCAGGGTGCTCCTGCTCTGCACTCCAGAAACACCCTGACAG160.6762468300929839No Hit
GTTTGTAAATTCAAGGTGCAAAAAGTTGATTTAAACCATTTGCAGAGTTGAACTCTATTATGAAAATAAAT160.6762468300929839No Hit
ACTGGAAACTTTCCATTTTATTCAAGAAATCTGTTCATGTTAAAAGCCTTGATTAAAGAGGAAGTTTTTAT150.6339814032121724No Hit
GCATTTAACGGATGCTGCCCTGACTGCTGGAGTGCAATGGCGCGATCTCGGCTTGCCGCAACCTCTGCCTC150.6339814032121724No Hit
CCTCACAGTTGCCATGTAGACCCCTTGAAGAGGGGAGGGGCCTAGGGAGCCGCACCTTGTCATGTACCATC140.591715976331361No Hit
CAAGCTCAAGGCCTTCCTGGCCTCCCCTGAGTACGTGAACCTCCCCATCAATGGCAACGGGAAACAGTGAG140.591715976331361No Hit
GATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATG130.5494505494505495No Hit
TATTTTTGATGACTATAAATAAGTGTTTCCACTATGGAAAAGAAAGTTAGCACAGTACATTTTCATGACTG120.5071851225697379No Hit
GCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGG120.5071851225697379No Hit
GGGAAGGGACGGCCCTCGCCCGCGGTGATGGTGGTTAGCAAGATGAACAAAGATGCGCAGATGAGAGCAGC120.5071851225697379No Hit
GGTTGCAGTAAACTGAGATTGCGTCACTGCACTCCCGCCTGGGCAACAGAGGGAGACTCAATCACAAAAAA110.46491969568892644No Hit
TTAATTTGCTCTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.46491969568892644No Hit
GGCGTTGGCGGCTTGTGCAGCAATGGCCAAGATCAAGGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGC110.46491969568892644No Hit
GGCTGAGGCAGGAGAATTGCTGGAACTCAGGAGGCAGTGGCTGCAGTGAGCCAAGATCACACCACTGCACT110.46491969568892644No Hit
GGATTTATGGAGGAGAGACTACCTAAGGGGAGCAGTAAAAATGAGAGTGCAGCTCTGCCCAGATGGCCATG110.46491969568892644No Hit
AGCCATGTGAGACACACTGATAGAAGATGTTAATAAGAGAGGAAACTAGGTTGCGGCTACATGGGAAATCT110.46491969568892644No Hit
CAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTAT110.46491969568892644No Hit
CATTTTTATATGACAATTCCTCAGGATTTGGTAAGGCTTCCAAGTTGTAGCTTTTAGTGTAAGTGCTGGGG110.46491969568892644No Hit
GTGTTCTAGGCCCGCCCACCCCAACCTTCTGGTGGGGAGAAATAAACGGTTTAGAGACTAAAAAAAAAAAA100.422654268808115No Hit
CCTCCTGAGCTGCTTGAGATTATCAACGAGGACATTGCCAAGAGGACGTGTGAGGAGCACCAGCTCTGTGT100.422654268808115No Hit
CAGCTATGTAACCTGTATTATTCTGGACGGACTTATTAAAATACAAACAGACAAAAAATAAAACAAAACTT100.422654268808115No Hit
GCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTTTGGATAAAAAAAAAA100.422654268808115No Hit
CCTTTAAGATCAGTTTGGCTGCAATTTTGGCTCCTTGGACCCTCAAGCATAGCTGGTAAATCAAGTTAGAG100.422654268808115No Hit
GCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACT90.3803888419273035No Hit
CACTGGCAGCTGACCCAGATGTTCCAGAGGTTCTATCCAGGGCAGGCACCTTCCTTAGCTGAGAACTTTGC90.3803888419273035No Hit
CAGCATTCTTATAGCTGTTTGTGATGATGCCAGTATTTGGCGCTGGGATCGACTTCGATAAAATACTTTTG90.3803888419273035No Hit
TCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.3803888419273035No Hit
CCTTTGTATCTCCTTAGCTGCTCAATAAATATTTGAATGCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.3803888419273035No Hit
ATAAAAAGGAATACATTGCAATCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2958579881656805No Hit
GAATGTCCGGGCCTTATATATCAAGAGCACCATGGGCAAGCCCCAGCGCCTATATTAAGGCACATTTGAAT70.2958579881656805No Hit
TCATTGGAGTGGGTGCTTGTGCATATATGCTAGGTATCTTTTGCCATCTTTCTCTTTAGATCAGGGGAAAT70.2958579881656805No Hit
GGTTTGTGAAATGGCTGCTGACATTTCTGAATCCAGCGGGGCTGACTGCAAAGGAGACCCAAGGAACAGTG70.2958579881656805No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2958579881656805No Hit
GTGTGTTCCAATAAATTATTTTGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2958579881656805No Hit
GTCCTCAAGACCCACGGACTCCTGGTCTGAGCCCAATAAAGACTGTTAATTCCTCAAAAAAAAAAAAAAAA70.2958579881656805No Hit
GGTGCTTGACAGAGTCACCCCATGGTGGTATGGCTGAACAAGGAGCGGCAGACAACTCAGGGAGAAACTCA60.25359256128486896No Hit
GTCCTTTGGGACAAAGCAGTATTTTACTCATTCTTTGAATGTTCTCATTCTTTTGTATCATGTGACTTATT60.25359256128486896No Hit
AGGCAGTGGCTCATTGTATGTGAGGTTGAACCACGTGAAATTGCCAATATTAGGCTGGCTTTTATCTACAA60.25359256128486896No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAG60.25359256128486896No Hit
GATACTAACAAAGACAGATTGGTGACTCTGGAGGAGTTTTTGAAAGCCACAGAAAAAAAAGAATTCTTGGA60.25359256128486896No Hit
ATTCCCAGTGCCCTGGACACCAACAGCTCGAAGAGCACCTCCTCCTTCCCCTGCCCGGCAGGGCACTTCAA60.25359256128486896No Hit
CAACCTCTTTATGCTGGATATTGTAACCATGAGAACATGTAAAAAATGGAGGGGAAAAAGGCCACAGCAAT50.2113271344040575No Hit
CTTTTCCTGCAAGGCCCCATGGGGCAGAATATAATAAATATTCTGAGTGAGAAAAAAAAAAAAAAAAAAAA50.2113271344040575No Hit
AAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGTATGAAGA50.2113271344040575No Hit
GTTTTAAACTTTCTAAATCATGGTGACTTCATTGAAAGCCATTAGTTGCTATTCTCTTAGGGCAGATAAAA50.2113271344040575No Hit
GATGATGACATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
ATTTCATTTCAGATACTTGTACGAAACACATTGGAGATAGACCCTGTGAATGTAAGCACTTGGTAAAACCT40.16906170752324598No Hit
CCTGTGTGTTGAGAGAACAAATCGTGGCTCAGTACCCCAGTTTGAAAAGAGAAAGTGAAAAAATCATTGAA40.16906170752324598No Hit
CCCTACACGACGCGGGCAATAAACCCCGTTTTTTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
GCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACG40.16906170752324598No Hit
CAGTGATCCCCACCCCAGGATCCTAAATCATGACTTACCTGCTAATAAAAACTCATTGGAAAAGTGAAAAA40.16906170752324598No Hit
GTCCTGCAGCAAACACTCCACCCTCCACCTTCCATTTTCCCCCACTACTGCAGCACCTCCAGGCCTGTTGC40.16906170752324598No Hit
GCTGGTTATACAGTTCAAGGCTTTCTATGTTGTTAATGAACCTCAAAATAGCCGTTAAGACATGAAATACA40.16906170752324598No Hit
GTTGATGTCCATGAGAAAGGATATGAGGACTAAACAGATACAAAATATGGAGCAGAAAGGAAAACCCACTG40.16906170752324598No Hit
GGAGGAACCCAAGTAGCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
ATGCTTAACATTGCTATACTACTTGTGTTGGTTAGGGGTTTGGATTTGGGGGTTCTTTGGTTTTAATTTTT40.16906170752324598No Hit
ACTTTGATACATATTGGAATACATTATGTTAATAAACTTGTAGCTTTTTGTGAAAAAAAAGAAAAAAAAAA40.16906170752324598No Hit
GATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACAGCTATGACAGAGGGGACTTGGAGAACCAGATGC40.16906170752324598No Hit
CCTCAGGGACAAAGAGATCTGGACAGAATCGCCGGACAGGTGGCAGCTGCCAACAAGAAGCATTAGAACAA40.16906170752324598No Hit
GTTTTTGGCTGTTACAAATGAAGCCTCTGTGAGCCACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
TAAAATAAACTGACTTATTTCACTAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
CACCTGCACCCAGGTGAAATAAACAGCCACGTTGCTCACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
GCAGAAGAATCACTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCATGCCACTGAATTCCAGCCT40.16906170752324598No Hit
TCCCTACACGACGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.16906170752324598No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA30.12679628064243448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA30.12679628064243448No Hit
GGTTTAACTCTGTAAGATACATCCACACATCACCAAGCAGTTTCACAGATAGTTTCTTTCTAGTTTTTGTC30.12679628064243448No Hit
GCTCTCCCACCTTTCATTTCTTCCTCCAGGCTTTACTCCCCTTTTTATGCCCCACCGATACACTGGGACCC30.12679628064243448No Hit
AGTCTGCATTTCATATTATTTTAGTGTGTAAAATTAATGTAATAAAGTGAACTTTGTTTTAAAAAAAAAAA30.12679628064243448No Hit
GGAGAACGGTGCTCATGCTTACAACCGGGAAGTGAATGGACGTCTAGAAAACGGGAACCAAGCAAGAAGTG30.12679628064243448No Hit
ATTTTAGTGTGTAAAATTAATGTAATAAAGTGAACTTTGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
GCCTATACAAAGAGGTATCTAGAAGAGCAGAAGACAGAGAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
TTCCCTACACGACGCGGGGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
GGTTCATACCTTATTTGCTAATCAAAGCCAGGGTCTCTCCCTCAGGTGTTTTTTATGAAGTGCGTGAATGT30.12679628064243448No Hit
CTCTTAGACATTCTCCTTGGTCCTCGTTCAGCTGCCCACTGTAGTATCCACAGTGCCCGAGTTCTCGCTGG30.12679628064243448No Hit
AAATATGATTAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
TCCGCCAGACTTTCGAAGCAGCCATCCTCACACAGCTGCACCCACGCTCCCAGATTGATATCTATGTGCAG30.12679628064243448No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
GCCCAGACTTGAGCCCAATTAAATTTTATTTTTGCTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
GGTTATCACTAGGCTAAAACTGGACAAAGACCGCAAAAAGATCCTCGAACGGAAAGCCAAATCTCGCCAAG30.12679628064243448No Hit
ATGTTCTGGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
GGGCCTTCTATGCTGAGCTTTATCATATCATCAGCAGCAACCGGGAGAAAATTGTCAACCCAAAGGGTGAA30.12679628064243448No Hit
ATACCAGTTCACTGGTATTAAAAAATATTTCAACTCTTATACTCTCACAGGTAGAATGAACTGTGTACTGG30.12679628064243448No Hit
CACTTGAGCCCAGGAGGTTGAGGCTGCAGCGAGCCATGATTCCACTGCACTCCAACGTGGGTGACAGAGTG30.12679628064243448No Hit
ATTGTGTTGTGCAGCATTAAAAAAAATCAAAATAAAAATTAAATGTGAGCAAAGAAAAAAAAAAAAAAAAA30.12679628064243448No Hit
GGGAGGCGGATAGACACGTGTTTGATATGAAGCCCAAGCACTTGCTGTCTGGGAAGAGGAAAGCTGGTAAA30.12679628064243448No Hit
CCAACAGTGATGGTCAGCTAGATTTCTCAGAATTTCTTAATCTGATTGGTGGCCTAGCTATGGCTTGCCAT30.12679628064243448No Hit
GTATATAGGTTTTGTACCTTTTTTACAGGAAGGTGACTTTCTGTAACAATGCGATGTATATTAAACTTTTT30.12679628064243448No Hit

[OK]Adapter Content

Adapter graph