FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4563
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139530.57199211045365No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT681.490247644093798No Hit
GCCTGGCTCAGCTGCTGGTGGATCAGATATACGAGAACGCCATGATTGCTGCTGGACTTGTTGACGACCCT481.051939513477975No Hit
GGTCAGGAATTCGAGACCAGCCTGACCAACATGGTGAAACCCCATCTCTACTAAAAATACAACAATTAGCC330.7232084155161078No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG310.6793776024545255No Hit
GCTGGCGAGACTGTAGCCCCCTCCCACTCCCACACTCACTCTTGCAGAGCCTGTGTCTTTAAGCAGCTGGC310.6793776024545255No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280.6136313828621521No Hit
GTTTGAGATCAGCCTGGCCAACATGGTGAGACCCCATCTCTACTAAAAATACAAAAAAAATTAGCCGAGCG280.6136313828621521No Hit
GGCTTTTCAAGTATGCTAGAATATTGCAAGCATTTAAATTCACATTTATCTGAAGGGATTTATTTATGTCA260.5698005698005698No Hit
GACCACTGCCTGCCTTGCTGGTCCTGCTCCTACCCTCATCTGCTCTCCCTGCACCCCACCAAGGCTCCTGC250.5478851632697787No Hit
GCTCTATGAATATCTACAAAATCGAATGATGGCCATTGCACCCAATGTTACAGTCATGGTTGGGGAATTAG240.5259697567389875No Hit
ATGTAGATGAGCTGGTATGAGAATATATGGAATTTTTAAGGGACTGTTTAAATCTTTGATTTGTAGACTAT240.5259697567389875No Hit
CGTTCCTTGTTGCCACTTTGGGTATTCTGAGATTTTCTCTTGCATGCCCTTAGCTTTACAGCTTTTGCATT240.5259697567389875No Hit
GGACTTGCTGAAGAAACAGAATATCAGTTCAAAACAAAACCTAGGCCAGGCATGGTGGTTCACGCCTATAA240.5259697567389875No Hit
ATGTCAAAAGTACCTTCTGTCCACACACACAAAATCTGCATGTATTGGTTGGTGGTCCTGTCCCCTAAAGA230.5040543502081963No Hit
ACAAACTGAAAAATTAACAACTGTTTTTATATCTGCAAGATAACTGAGGTTACAGGGCAAACCACTACCCC230.5040543502081963No Hit
GCCAGGGGAGCACCCCACTCAACTACTCAGAATTTTAAACCATGTAAGCTCTCTTCTTCTCGAAAAGGTGC220.48213894367740523No Hit
CTCTGGAGTTCCAAAGATGTCAGAGATATGGATGACGCCTATGACAGACTGACAAGGCACCGCTGCAGGAT210.46022353714661407No Hit
ATTCTAGGAGAAAGAGAAGGTCTATGTCAACAGAGTTGTTATCTCATAGAGCCAGTTTTCAAAGCTCCTTC200.43830813061582297No Hit
CTGCTCTGTGACCAGGCCAGGATTCCTGGAGCTGGCCTGAGAGGGCTCAATGGACCCTCGGGGACCCAAGT200.43830813061582297No Hit
GTGTAAAGAATTATGTTACAAATGCTATTCACTAATTCTTAACCTAACTGGAATACTGAGATGTTAACTAC200.43830813061582297No Hit
GGAGGAAGACAAGAAGTGAGACTGGAGGGAAAGGGTAGCTGAGTCTGCTTAGGGGACTGCATGGGAAGCAC200.43830813061582297No Hit
GACCTTCATTCTGCCTTGGATTGGCTCTGTTTAAACCTTTCAGATGATGCACTTCCTGAAGGATTCAGTCA200.43830813061582297No Hit
GATGGTGATAGTTCCAAAGTGAAGGTTAAAGTTCGTGTTAACATCCATGGAATCTTCAGTGTGGCTAGCGC200.43830813061582297No Hit
CCCGGATACAGCAGCAGCTGGGCCAGCTGACACTAGAAAACCTTCAGATGCTGCCAGAGAGTGAGGATGAG200.43830813061582297No Hit
GGACCATTCTGATCATCCTCACTGGACGCCACAGGGGCAAGAGGGTGGTTTTCCTGAAGCAGCTGGCTAGT190.41639272408503175No Hit
AGCCCGCGCCAGTCCGACGTCATGATCGTGGCCGGCACACTCACCAACAAGATGGCCCCAGCGCTTCGCAA180.39447731755424065No Hit
CATCCAAACTGTATCTTCCTTTACTGTTTATATCTTCACCCTGTAATGGTTGGGACCAGGCCAATCCCTTC180.39447731755424065No Hit
GCTCGCCTCAGGAATGGGCTGTTTTTGGCCTAACCCTCAGAAACACTGGGGCTGGCACAAACTCTTGGTTT170.3725619110234495No Hit
AATCCCAGCTACTCAGGAAGCTGAGGTAGGAGAATTGATTTAACCCGGGAAGCAGAGGTTGCAATGAGCCA160.35064650449265833No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA150.3287310979618672No Hit
GACCACCCATTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAGGACCAGATCAACAGGCTTATTAGAAGAA150.3287310979618672No Hit
GTAGATAACCAGATTGTTGCTTTTTGTTTAAACCAAGCAGTTGAAATGGCTATAAAGACTGACTCTAAACC150.3287310979618672No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC150.3287310979618672No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCC150.3287310979618672No Hit
GCACTCCTGTAACAAACATTATTTTTCCATTTCATTGTATTGTGTTTTGCCATTGTTGGTCTGTTGATTTC150.3287310979618672No Hit
GCCTGGGAGGTAGAGGCTGAAGTGAGCAGTGATCACGCCACTGCACTCCAGCCTAGGTGACAGAGTGAGAT140.30681569143107607No Hit
TACTCGGGAGGCTAAGGCAGGAGAATCGCTTGAAGAGCAATCCCAGCTACTTGGGAGGTGGAGGCTGCAGT130.2849002849002849No Hit
GCTGTGAAAGCAACATAAATGGATTTTAAACTGTCTACGGTTCTTAACCTCATCTGTTAAGTTCCCATGCC130.2849002849002849No Hit
GCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGCTATAAAATCACCACGGTCTTTAGCCATGCA130.2849002849002849No Hit
GGTCTATCTCATGAAACCTCTTCTCAGACCAGTTTTCTAAACATATATTGAGGAAAAATAAAGCGATTGGT130.2849002849002849No Hit
ACTTAAATGTCATAAAGTAAATGAAGATAAACTTTTCATTAAAATACTTTAGAAATTTTTGCATTTATGTT130.2849002849002849No Hit
CACTGTAGATGCTATAATAAAAATAGCTGTTTGGTAACCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2849002849002849No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.26298487836949375No Hit
GGTCAGGGCTAAGGCCGGGGATGAGACTGCAGGAGAGAGAGCAGCGGAGGGCCACATTCGGAGCCTCCGTC120.26298487836949375No Hit
CCCCCTGCTGGTGTCTAGTGTTTTTTTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCA110.24106947183870261No Hit
CAAAGAAAAGCAAGATCTAGACAAGCAAGACAATCTAGAAGATCAACACAGGGAGTGACATTAACTGATCT110.24106947183870261No Hit
GTGGCCTGTTCAAGGCAGAACCACGACCCTTGACTCCCGGGTGGGGAGGTGGCCAAGGATGCTGGAGCTGA110.24106947183870261No Hit
CTGCCAAATAAACAGCTATTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.24106947183870261No Hit
CAATAAAGTACCCTGTGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.24106947183870261No Hit
TTCTTATCACATTTTCGACTTCTTCACTTGACCTAACTGATTATGCGAAATACCCAAGATTCATGCTACTG110.24106947183870261No Hit
ACTTTATAATAATTATAAATAAACCTGTTAAATTTTTCTGGACAATGCCAGCATTTGGATTTTTTTAAAAC100.21915406530791148No Hit
CTCAAAGACAGCCAATAAATTCTGTTCAATCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.21915406530791148No Hit
TCAGCTACACTTTGTTTTTAAGTTTGTTTTTGACATGTTTATTTGGCAAATTTTATAATGAAGTTTTAAGT100.21915406530791148No Hit
GCACAGGTGCTGTATGTCTTAGATATACAGCACAGGTGCACAGGTCTTAGATATACAGCTCAGTGAAGCTT100.21915406530791148No Hit
GCTACACCCTTCCTTGTAGTAAGACACCAACTGCTTAAAACATAAGGATGTTTCAGTTCCTCCATTTAACA100.21915406530791148No Hit
GATTGAGAAAATAAAATCAGATTTTTTCAAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.21915406530791148No Hit
TCCCTACACGACGCGGGAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.21915406530791148No Hit
GGTTGGAGCTGCTGAGACGACGCTCACAGAGCTGAGACGTACAGTCCAGTCCTTGGAGATCGACCTGGACT100.21915406530791148No Hit
GTGGAGGAAGAAGCGAATGCGCAGGCTGAAGCGCAAAAGAAGAAAGATGAGGCAGAGGTCCAAGTAAACCG100.21915406530791148No Hit
GTTGCTATTAAATGGACTTCGTGATTTTTGTTTTGCACTAAAGTTTCTGTGATTTAACAATAAAATTCTGT100.21915406530791148No Hit
TCTGCACTACTACACCCTGCATGCTTCGAAACTCTGACAGCATACTGGAGGCCATTCAGAAAAAGCTTGGA90.19723865877712032No Hit
GCGCTGTGCCTGCTCGTGTTGCCTACATGTCCCTGGCTGTTGAGGCGCTGCTTCAGCCTGCACCCCTCCCT90.19723865877712032No Hit
GGATTATTGAGTATTCACACACGTGTGTGAGATGTGCCACCCTTGAACCTTGTTACAACGTGGGCACATTA90.19723865877712032No Hit
TCCCTACACGACGCGGGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.19723865877712032No Hit
ACATACAATCAATGTAAAGCCATATCACAATGATACAGTAATATTGCAATGTTTGTAAACCTTCATTCTTA90.19723865877712032No Hit
GGTTAGAAGATGCCCGCCAGCATTCCAATTCCAACATGGTCATTATGCTTATTGGAAATAAAAGTGATTTA80.17532325224632916No Hit
TTTAAAGCCCTCTCCTTTAGAATTTAAAATATTGTACCATTGAAGAGTTTGGATGTGTAACTTGTGATGCC80.17532325224632916No Hit
GGAGGTTGAGGCTGCAATGAGACCCAAGATCTCACCACTGCACTCCAGCCTGGGTGACAAAATGAGACCCT80.17532325224632916No Hit
GCAACGGGCTGCTCTACGCCGGCTTCAACCAGGACCACGGATGCTTTGCGTGTGGGATGGAAAATGGATTC80.17532325224632916No Hit
TTCCTAGTGTTTGTGAAATAAACTTGGTATTTGTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.17532325224632916No Hit
ACATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.17532325224632916No Hit
GGTCTAGGAGGGGCATTTTGGAGACTGCCTGACACCACCCCTATCCCCTGCCTCCCCCTCTCAGAAGAGGG80.17532325224632916No Hit
CCCCCGGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTGTATTATATTAAAATAC80.17532325224632916No Hit
ATAATCCATAAATATATAAAATTATTACTTGTCAATGAAAAATAAAACTTTAAGAGTTTTGAAAAAAAAAA80.17532325224632916No Hit
GCTTGAATCCGGGAGGCGGAGAGAGGTTGCAGTGAGCCGAGATGGCACCATTGTACTCCAGTCTGCGTAAA70.15340784571553803No Hit
GGGTACAATCGTGTGCTGCCTGGAGGAGAAGCCTGGAGACCGTGGCAAGCTGGCCCGGGCATCAGGGAACT70.15340784571553803No Hit
CGTTTTGTTAGAAATGTCAGATAGGAAAATAAAAACCATTTGAGCAAAAAAAAAAAAAAAAAAAAAAAAAA70.15340784571553803No Hit
ACGCTCTCCCCGACACATTTGTTAAAATCAAACCTGAATAAAACTACAAGTTTAATATGAAAAAAAAAAAA70.15340784571553803No Hit
GCTGGGCGCCCGGGCCCGCGCCGAGCCCGCCGGGAGTGCCGTCCCCGCGCAGAGCCGCCCATGCGTGGACT60.13149243918474687No Hit
GGCTCCGTAACTTTCTATCCGTCCGCGTCAGCGCCTTGCCACCCTCATCTCCAATATGCCTGGTCCGACCC60.13149243918474687No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA60.13149243918474687No Hit
GCCCCAGCTAAGGTTGAAGCCAAGGAAGAGTCGGAGGAGTCGGACGAGGATATGGGATTTGGTCTCTTTGA60.13149243918474687No Hit
CCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGGAGAACCTGAA60.13149243918474687No Hit
CTACTCTTTGATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13149243918474687No Hit
TTATTCCTATAAGAATTAAATTGAGTTTAGAGAGAATGGTGGTGTTGAGCTGATTATTAACAGTTACTGAA60.13149243918474687No Hit
CAATTTTTGTTGTGTTTGATAGCATTGAATCTGCTAAGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAA60.13149243918474687No Hit
GTACGAGGCCCTGACGCCAGTTCACTGAGACTGTGCCTGTATATGAACTTTGAAAAAATACTTGACTCTAC60.13149243918474687No Hit
GCAGTAATACAGTCTATACATTGTTCTGTTTTCAAATTTATCCTGAGGCTTTGTTGAGCATAAATGATTAT60.13149243918474687No Hit
CAATAAAACAAGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13149243918474687No Hit
GAGAACATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13149243918474687No Hit
AGGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13149243918474687No Hit
CTATTGAACTTGAAACCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13149243918474687No Hit
GGAGGTGGAGGTTGCAGTGAGCCGAGATCGCGCCATTGCACTCCAGCCTGGGTGACAGAGCGAGACTACAT60.13149243918474687No Hit
GAGTCAGAAAGCTAAATAAAAAAATGAAACTTTTTTGAGTAATAAAAATGAAAAGACGCTGTAAAAAAAAA60.13149243918474687No Hit
TATCAGAATTCACAAATAAAGCTTTATTGGAACTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13149243918474687No Hit
GGACATGAAGGTGAACCCCATCCAGGGCTTAGCCTCCAAGTGGGACTACGAAAAGAACGAGTGGAAGAAGT60.13149243918474687No Hit
GGATATCTATAGAGAAATGGGGTTTGGTCACTATGAAGAAGAAGAAAGCTGTTGGGAGAAACAAAAAAAAA60.13149243918474687No Hit
GCCGCACACAGGCCTAGAGGTAACCAATAAAGTATTAGGGAAAGGTGAAAGAAAAAAAAAAAAAAAAAAAA60.13149243918474687No Hit
GAATAAAAAAGATTCTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10957703265395574No Hit
CAACTGTCATATTTTGATGTCAACAGAGTTTTAGGGATAAAATGGTACCCGGCCAACATCAAGTGACTTTA50.10957703265395574No Hit
GTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTT50.10957703265395574No Hit
GTGATTACATAGATCAGCCATTAAAATAAAACAAGCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10957703265395574No Hit
CATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGG50.10957703265395574No Hit
GATTTAAATATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10957703265395574No Hit
GTCTCGCTTGGCTCTCATGACTGTGGTGGTGGAGATAGCGTGGGGAGCCTCGCCCATGGTCTCACGTGGCA50.10957703265395574No Hit
GCCTATGCCATGAAGCGGGACCTCACTCAGATGGCCGACGAGCTGAGGCGGCACCTGGAGCTGAAAGAGAA50.10957703265395574No Hit
GCTATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCATGAGCCCTACAAACAAC50.10957703265395574No Hit
GAACCAAGCTGGGTCTATAAGGAATTGCACATGAGATGGCACACATATTTATGCTGTCTGAAGGTCACGAT50.10957703265395574No Hit
GAGCCATATGTAATGTACTGTAACAAAGGAGCTTCTTGTCCCCTTGGTCTTTTAATTAAAAGAAATTCCAA50.10957703265395574No Hit
CTCAGATGCAGTTTGGAGAGTCAGATCTTTCTCCTTGAATATCTTTCGATAAACAACAAGGTGGTGTGATC50.10957703265395574No Hit
GGCCAGTTTGTGTATTGCGGCAAGAAGGCCCAGCTCAACATTGGCAATGTGCTCCCTGTGGGCACCATGCC50.10957703265395574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)