FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5202
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156029.988465974625143No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG661.2687427912341407No Hit
ACAATGTGCTGTGAAAAATCTGATGACTTTAATTTTAAAATCTTGTGACATTTTGCTTATACTAAAAGTTA430.8266051518646674No Hit
GGACAATTTCTACAATTTTGCTTCATCATTTGCTGTCTCTCAGGCCCAGATGACACCAAGCCCATCTGAAA380.7304882737408689No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360.6920415224913495No Hit
ATATTCATATGTGCTAAAAATAAAGATTTTATCAGCTCATGATAAAGGTACTCATGTTGAGGTCAATGTGA360.6920415224913495No Hit
CAGTAAAGTTTGAAATATGGGATACAGCTGGTCAAGAACGATACCATAGCCTAGCACCAATGTACTACAGA290.5574778931180315No Hit
GTATCATGCGATGGTCCCCCACACCGGTTCCCTGAACCCCTCTTGGATTAAGGAAGACTGAAGACTAGCCC270.5190311418685121No Hit
CCTTTAGAAGGACTTAGACCTCACATATTAATGTTGAGAAGTTCTGCTTAATTTTAAAATGGTTTCTATAA240.461361014994233No Hit
GCCTTCAGGATATACTGATTCTACTGCTCTTGAGGGCCTCGTTTACTATCTGAACCAAAAGCTTTTGTTTT230.44213763936947326No Hit
GGGCAACAGAGCGAGACTCCATCTCAAAAAAAAAGGAGACTTCATGTGCCCCCAATTTTTCACTATTGTTA230.44213763936947326No Hit
GGTGGAATTAAGAAATCTGGCAAAAAGTAAGCTGTTCCTTGTGCCTGAGGAGATGGTGACCCTTTATTTCA220.4229142637447136No Hit
GTATTTTATAGGTCATAAAAAACACCATAATATAACGAATCTCATTTTCTTTAAATGTGAATTAAATCCTA220.4229142637447136No Hit
GTGTGAGGAGGAGGCCGCGGTGCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACC220.4229142637447136No Hit
CTACAGGAAAGTACTGCTTGTTCTCTGTGGAGGAGATGATTAAAATAAAAATCCCAGAAGGACAGGAGGAT200.3844675124951942No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT200.3844675124951942No Hit
GGAGGTGGCCGAATTGACAAACCCATCTTGAAGGCTGGCCGGGCGTACCACAAATATAAGGCAAAGAGGAA190.36524413687043444No Hit
GGTGACCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCTGGGCGAGTATCTCTTCGAAAG190.36524413687043444No Hit
GTAACACTGGCAACTTTGAAAATGTCTGTACAGCCAACGGTTTCCCTTGGGGGCTTTGAAATAACACCACC190.36524413687043444No Hit
TGTTCTTAGTGGCCAAGTGCCAGGTGGCTTCAGCAGCTTCCTACGATCAGCCGAAGAAAGCAGAAGCTCTG190.36524413687043444No Hit
CTCTACTCCCTCTGATAGAATAAATCATTCACCTCTGTGCCCCCAGAGCACTTGTAACAGCAGGATATAAG190.36524413687043444No Hit
CTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.34602076124567477No Hit
CTAGAATATAGACTGTCTGTATTATTATTCACCTATAATTAGTCATTATGATGCTTTAAAGCTGTACTTGC180.34602076124567477No Hit
GCCCGACGAAAGGATGCCAAATCTGTCAAGATCAAGAAAAATAAGGACAACGTGAAGTTTAAAGTTCGATG180.34602076124567477No Hit
GTCCATCTGCTCTTGACTCTGAGCTGGCTAGGTGACTGTTGGTTATTCCTGGGACAGGTGCTGGGTAGGCC180.34602076124567477No Hit
GTCTACTGTTCTATCAATTGATTTCAAACCTTCAGAAATAGAAGTTGGAGTAGTGACAGTTGAAAATCCTA170.32679738562091504No Hit
GGATCCTGTCACACCTGGAGACCTACAGGAGGCCAGAGTGAAGGGGTCCGTTCTGTCCCTCACACTGTGAC170.32679738562091504No Hit
GTTTTAATTAATTCCCATACTGATAAAAATAACTCCATGAATTCTGTAAACCATTGCATAAATGCTATAGT170.32679738562091504No Hit
CCTCTGGAGGTGCAGGGCACCCTTGAGAAGCCCCTCACCCCTAGGCCGCCTCCAGGTGCTACCCAGGAGTC160.30757400999615536No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA150.28835063437139563No Hit
GCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTAC150.28835063437139563No Hit
GCTACTTGGGAGGCTGAGACAGGAGAATCCCTTGAACCTGGGAGGTGGAGGTTGCAGTGAGTCGAGATCAT150.28835063437139563No Hit
GGCTCACTGCCATTGTCACCAGGTTGGAACTCTTGCCTCTGTGAGGATGCCTTCTCTACTGTGCATACCCA150.28835063437139563No Hit
GTGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAAAGACTGGTTAATG150.28835063437139563No Hit
GGATTACCCCTTCCATGAAGAGTTTTGAAGCTGTGGTTGCCTGCTCGTCCCCAAGTGACCTATACCTGTGT140.2691272587466359No Hit
GAGTAAGCTGCCATTCTTCTGTAACAATGTTATCAGTAATGCTTTAAACTCCAGCACCTGGTTATGCATTT140.2691272587466359No Hit
GGAGCTTACTAATGATGAACCACCCGACCAACACGCACTGGTACCACAGTCTTCCCGTGAGAAAGAAGGTT140.2691272587466359No Hit
AGAGAAATGCCTACTTTTTCAGGTACATTGGAAGGTCATCTGATTCCTATGGCAATTCTTTTAGGACAAAC130.2499038831218762No Hit
ACATTTAAATATGATCTTGGGAGATGTGGAAGAAACTGTGACTACTATAGAAATTGATGAAGAAACATATG130.2499038831218762No Hit
ATGTAGATGTGATCTCAGTTGTAAATAGAAAAATCTAATTCAATAAACTCTGTATCAGCCCCCAAAAAAAA130.2499038831218762No Hit
CAGACCAGTGAGCACATTCTTCCTCTTCCCAGTTCACAAAACCCCCAGATTCAGCCTCCTAGTTGGCAACC130.2499038831218762No Hit
CCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACACCTATCCCCCATTC130.2499038831218762No Hit
GTTTGATTCTTTTTTAATAAACTACTCCTTGATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2499038831218762No Hit
TCTGCAGTACATTGTGACCTGGTATGGACTCTCTGCAGCTACATCCTACCTGTGGTTTAAGAAATTCCTAC130.2499038831218762No Hit
GGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCATCATAATC130.2499038831218762No Hit
CCGCACACAGGCCTAGAGGTAACCAATAAAGTATTAGGGAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAA120.2306805074971165No Hit
CATATAGCCTGTGACAATAAAGATTTTTTAAAACAGTTACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.2306805074971165No Hit
AGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATA120.2306805074971165No Hit
GGGTATGGTGGCACACACCTGTAATCCCAGCTACTTGGGAAGCTGACGCAGGAGAATTGCTTGAATCCAGG120.2306805074971165No Hit
GGGCCAAAGGAATAAGGAATGTGCCATACCGAATCCGTGTGCGGCTGTCCAGAAAACGTAATGAGGATGAA120.2306805074971165No Hit
GAGTTTTTTTGTGTGTGGTTTTTAAAACTGTTAAGGCAAGAAGTGTCAAATGCTTTAGAGTTAAATAACAG120.2306805074971165No Hit
ATGCAATATATAGTTTGTCCAGATTAGCTTTCATTTGGGGAATGAAGTTCTGAAATATCTAAAGCAGTTTA120.2306805074971165No Hit
GCTACTCGGGAGACTAAGGCAGGAGAATCGCGTGAACCTGAGAGGCAGAGGTTGCAGTGAGCTGAGTTAGT120.2306805074971165No Hit
ACAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGG120.2306805074971165No Hit
GTTTTATGACTTCATTAATAATAAATTCCCTGGCCTTTCATATTTTAGCTACTATATATGTGATGATCTAC120.2306805074971165No Hit
ACTCCGGAGGCTGAGGAAGGAGAATGACGTGAACCCGGGAGGCGGAGCTTGCCGTGAGCCGAGATCGCGCC120.2306805074971165No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.2306805074971165No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2114571318723568No Hit
GGCTAATTTCCCCATAGCCGTGGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT110.2114571318723568No Hit
GCTTTGGAAGTTTAGCTCTGAGAACCGTAGATGATTTCAGTTCTAGGAAAATAAAACCCGTTGATTACTAA110.2114571318723568No Hit
GATGTATGCCAAGCGTGCCTTTGTTCACTGGTACGTGGGTGAGGGGATGGAGGAAGGCGAGTTTTCAGAGG110.2114571318723568No Hit
GGGGCCATAACTCATGGGAAAACAGAATTATCCCCTAGGACTCCTTTCTAAGCCCCTAATAAAATGTCTGA100.1922337562475971No Hit
CTGCTTTAGGATGACACAATGAATAACACCTAGTCATAGAAATCAGTCTCTCTGGTTTGTTTTGTATTATG100.1922337562475971No Hit
GGGCAACAGAGCGAGACTCCATCTCAAAAAAAAAGGAGACTTCATGTGCCCCCAATTTTTCACTATTGTTT100.1922337562475971No Hit
TTACAGACATGAGCCACTGCGCCTGGCAAAACTTTGTAATTTTGAGTTTTGTGATGATTCCCAGGCCTTCT100.1922337562475971No Hit
GCTCTATTTGGATTAAGCAGTTTGGAAACTCTGTTTTTTTCCATTCTGTGAGTGGACATTTGGGAACTCAC100.1922337562475971No Hit
GATAATAGTCACTTCCATTGAAGAATAAGCAAAACTTCCAAAGACTTCATTAATATCCACATCAATGATAT100.1922337562475971No Hit
GGAGTTCAAGACCAGCCTAGACAACACAGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCAGGCA100.1922337562475971No Hit
AGTCAAACATTTCAAAGTTTGGATTGTATCAAGTGGCATGTGCTGTGACCATTTATAATGTTAGTAGAAAT100.1922337562475971No Hit
CTCAGTAAAATTGGAGAGGATTCTTTTGCATTGAATAAACTTACAGCCAAAAAACCTTAAAAAAAAAAAAA100.1922337562475971No Hit
TGTAAATAATTTCCATATTTCTCTTTTATAATAAACTAATGATAACTAATGAAAAAAAAAAAAAAAAAAAA90.17301038062283738No Hit
GCTGTGCCAGGTTGAAGTATAGTGGAACCCATCAGTAGTAATGTGTAGTAGTTATGACTTGTTGACATTTC90.17301038062283738No Hit
CAAAGTCCCAGAACCCACTGCGCAGCTTCAAGCACAAAGGAAAGAAATTCAGACCCACAGCCAAGCCCTCC90.17301038062283738No Hit
TATATATATATAAAAATTGGGTCTTAGATTTTGATTTACTAGTGTGACAAAATAACTACATCCTAATGAAA90.17301038062283738No Hit
GACAGATTACAATTCTATTTTAAATATAAAGTTTATAAAATAAATACTTTTTGTATCCAAATACTTGGTGT90.17301038062283738No Hit
GGCAGGAAGAATCTCTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCAAGATTGTGCCACTGTATTGCAGC90.17301038062283738No Hit
GTAATCTAAATACGTGTTAACAGACTAAACTGACCAACTCTAACAGCAGCTATAGGCCATTCAACAGTCCT90.17301038062283738No Hit
CACCAGGATTTATACATACAGAGATTGTAGTATTTAGTATATGAAATATTTTGAATACACATCTCTGTCAG90.17301038062283738No Hit
ATATTATACTACTCTAAATAGCATATTTCATCCAAGTGCAATAATGTAAGCTGAATCTTTTTTGGACTTCT90.17301038062283738No Hit
GGGGTTGGCTCCATGACCAAGATCTATGGGGGACGTCAGAGAAACGGCGTCATGCCCAGCCACTTCAGCCG90.17301038062283738No Hit
GCCCTGCCCCCATGGGCTTTACCCTTCCCTGCGGGCTCTCTCCCCGACACATTTGTTAAAATCAAACCTGA90.17301038062283738No Hit
TGGCAAGACCAGCAAGAAGATCACCATTGCTGACTGTGGACAACTCGAATAAGTTTGACTTGTGTTTTATC80.15378700499807768No Hit
GGGTCCGGGGCGGAGTGCCGTTGGTTAAGGAGAAGCCAAAAATGTTTGCCAAGGGAACTGAGATCACCCAT80.15378700499807768No Hit
GGTATATAAGTGTGATCCTGCAGGTTACTACTGTGGGTTTAAAGCCACTGCAGCGGGAGTTAAACAAACTG80.15378700499807768No Hit
TTGTAGAGCCATATATTGCATGGGGGTACCCCAATCTGAAGTCAGTAAATGAACTAATCTACAAGCGTGGT80.15378700499807768No Hit
GAAGAGACTTGGCATTCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.15378700499807768No Hit
GCTTTTATGAGAGCAGCTGTGCAAGTGAATCCACATTAATGCCTTGTCGTGGTGCCATTCCCAGCGCCTGA80.15378700499807768No Hit
CACACAGGAGGGAAAATCCTGGGATTCTTTTTCTAGGGATGTAATACATATATTTACAAATAAAATGCCTC80.15378700499807768No Hit
GTGTACACAAGATTTCTGGAAAAGTAAAGAAAAACCCTTTTTATGGCTCACACAGCTTAAGAGTAGCTGTC80.15378700499807768No Hit
GGAGAAAAAGCTGAACCAGGCCCTTTTGGATCTTCATGCCCTGGGTTCTGCCCGCACGGACCCCCATCTCT80.15378700499807768No Hit
GGTTAGGTTACATCTGGTTACTGTCCTGGGAAAATCATTTTTATAGAGATGGCCTTCCAAGTGGTTTTAAA80.15378700499807768No Hit
GCTCACTTTGGAGCCACTGAAGAGCCGACTGTGCCACCCACTTGCGAGCCTCAGTCCCTTCCAAGCTGCAC80.15378700499807768No Hit
GGGAAAATAAATTACACTGAAGCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.15378700499807768No Hit
TTTATATTCTTTCCATTTGCTATCATGTCAGTGAACGCCAGGAGTGCTTTCTTTGCAACTTGTGTAACATT80.15378700499807768No Hit
AAAGTACCCTGTGCTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.15378700499807768No Hit
GGGTTAATGGCTAGTACTGTATTGGCTCTGTGAAAACATATTTGTGAAAAGAGTATGTAGTGGCTTCTTTT80.15378700499807768No Hit
CTGTGCTGTGTATGTGAACCACCCATGTGAGGGAATAAACCTGGCACTAGGTCTTGTAAAAAAAAAAAAAA70.13456362937331795No Hit
GGTGTAGCCCTCCCACTTTGCTGTTCCTTACTTTACTGCCTGAATAAAGAGCCCTAAGTTTGTACTAAAAA70.13456362937331795No Hit
GGACTGTTCCACTGCCCCTCCTCCTCACCCTATCACCCATGGATCGTAATGTAAAATTCTTTTACCATGTC70.13456362937331795No Hit
GTGTACAGTCGCCAGATAGGGACCGGTGAACACCTGATTCCAAACATGTAGGATGGGGGTCTTGTCCTCTT70.13456362937331795No Hit
GTATGAGCACGACTTAGAGGTTGCTCAGACAACTGCTCTCCCGGATGAGGATGATGACCTGTGAGAATGAA70.13456362937331795No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13456362937331795No Hit
GCCTAATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13456362937331795No Hit
GTATGTGAACCACCCATATGAGGGAATAAACCTGGCACTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13456362937331795No Hit
GATCTAAAATCCATCCTGGATCATGGCATTTAAATAAAAGATTGAAAGATTAAAAAAAAAAAAAAAAAAAA70.13456362937331795No Hit
CTGTACAGTGCGCGCCAGCGGCGGCGGCTGAACCGGGGCCTGCGGCGGAAGCAGCACTCCCTGCTGAAGCG70.13456362937331795No Hit
GGAATAAAAAAGATTCTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13456362937331795No Hit
CCCTACACGACGCGGGGTACACTGACTTGAGACCAGTTGAATAAAAGTGCACACCTTAAAAAAAAAAAAAA70.13456362937331795No Hit
GACCAAGCTTCCACTGACAAGGAAGAGGATTATATTCGTTATGCCCATGGTCTGATATCTGACTACATCCC70.13456362937331795No Hit
CTGGGGACTAGGTGAGAGCTGTGCCTGCAAGAGAGCTTCTTGGGCATCTCAAAGGAGACACTCCACAAAAA60.11534025374855825No Hit
TGGTAAGCCAAGATCAAGCCATTGCACTCCAGCTTGGGCAACAAGAGAAACTCGAAAAAAAAAAAAAAAAA60.11534025374855825No Hit
GCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGAAATAAAGCTTTTTGATGTAAAAAAAAAAAAAA60.11534025374855825No Hit
GCGCCAGCCCTACACTCGCCCGCGCCATGGCCTCTGTCTCCGAGCTCGCCTGCATCTACTCGGCCCTCATT60.11534025374855825No Hit
CCAGTGGACAGATGAAGAATTAGGTATCCCTCCTGATGATGAAGACTGAAGGTGTAGACTCAGCCTCACTC60.11534025374855825No Hit
GAATTTTTGCTGCACATGCTTAAAAACGCAGAGAGTAATGCTGAACTTAAGGGTTTAGATGTAGATTCTCT60.11534025374855825No Hit
CCATGAAAGCTGCCATGGCCCTGGAGAAAAAGCTGAACCAGGCCCTTTTGGATCTTCATGCCCTGGGTTCT60.11534025374855825No Hit
GCATTCAGGAAGTGCGGGACATGGTAGGGGAGGCAAAAAGCCTTGGGCACTACCCTCCCTGTGGAGCTGTT60.11534025374855825No Hit
GGATGAGGTACTTGAGAAGTTGGAAAGGCACAATGAAAGAAAAGAAAAACTAAAACAACAGTTGAAAGGAA60.11534025374855825No Hit
CCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11534025374855825No Hit
AGATTACACTGTATTAACTCTCGAGGAGTTTCCTCACCAATAAACAGACAACCTCAAAAAAAAAAAAAAAA60.11534025374855825No Hit
AGCGAATGTTCTGAGGACATTCTAGACAACAGCTTAGTTCCTTTTTCAGGCTCATTTGCTTTTGCTTTTTT60.11534025374855825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)