FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA271.8072289156626504No Hit
GGATATTGAGTATCCATTGAAATGGATTTGTTCAGCCATTTACATTAATGAGCATTTAAATGCAACAGATA231.5394912985274432No Hit
ACAAAACACATGCATCTAAACTGCTGGGCAATCCAGTTGACTTTTAAATGTAAGAATGGAATTCCAAACAC231.5394912985274432No Hit
GATTGACGGACATCCTGTTAAAATAAACGGCCTACACTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221.4725568942436411No Hit
GTCTGTACCAGTAGACCAGTACCCTAACTACTGAAAAGAATATGGCAGTTTTCCATTTGTCTAGTTTCTGT221.4725568942436411No Hit
TCCCTACACGACGCGGGAGAACGCCCCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211.4056224899598393No Hit
GCATTGTCTCATGCCTGTTTGTGTTGGATGCCTTGTGCTAAGCTGTAACAAAATGTTGCTTCTTTTGCTGT211.4056224899598393No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCACTCTATTGACTCCTAGCCGCAG211.4056224899598393No Hit
GTACCTTCTTGGTCAAATTTATTCCTAGGTATTTTATTCTTTAGGATGCTGTTGTAAATAGAATTTTTTTC201.3386880856760375No Hit
GCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGA201.3386880856760375No Hit
TTTTAAAATGAGCACAAAGAATGTTGAAGTTCAGATTAATCTCTTCTGAATGATGTTTTTTTCCTCTGTGA191.2717536813922357No Hit
CTACAAATTGTGTGCAGTGTATACTGCTTGGGTGATGGGTGCACCAAAATCTCACAAATCACCACTGAAGT191.2717536813922357No Hit
GTATAAATCCAATATCATGTCTTGTGCATAATTCTTCCAAAGGATCTTATTTTGTGAACTATATCAGTAGT181.2048192771084338No Hit
TTATCAGTCTGTACCAGTAGACCAGTACCCTAACTACTGAAAAGAATATGGCAGTTTTCCATTTGTCTAGT171.1378848728246318No Hit
GCCCAGGAAGTTGACGGTGCAGAGATCCCTCCACTGAACTCCAGGCCGGGTGACAGCAGAGCGAGATCCTG171.1378848728246318No Hit
CTCTGTAACAGAAGGGAAGAAAAAAGTTCCTGTGGCTGGCTGCAGAGTCCAAAAGGGACAGTTAGAAAAAC161.07095046854083No Hit
ACATAGTTTTATGCAGTAAGTATTGCAATAGAATCCTGAAAATTGAAAAAAAAAAAAAAAAAAAAAAAAAA151.0040160642570282No Hit
ACTAAATGGTGTTTGTAGCATTTTTATCATACAGTAGATTCCATCCATTCACTATACTTTTCTAACTGAGT151.0040160642570282No Hit
GACTGATACTACAGTGCCTCGCCGCCTGGGCCCCAAAAGAGCTAGCAGAATCCGCAAACTTTTCAATCTCT151.0040160642570282No Hit
CCTTTACATTCAGGAGGTAGCAGATTACCTGACACCCCCAGTGGCAGCCTTGTTCCTGCTGGCAATTTTCT151.0040160642570282No Hit
TCCCTGCTGTTACCTAATTACAAATTGGATGGAACTGTGTTTTTTTCTGCTTTGTTTTTTCAGTTTGCTGT130.8701472556894244No Hit
CCTTTATTTTATGAGATATTTATGTAAAAATAATTTTCATGCATATTGTATTAAAGCTTAATGCCAGTCCT130.8701472556894244No Hit
ATCTTACTGTGCCGAGATTGCTCACAATGTTTCCTCCAAGAACCGCAAAGCCATCGTGGAAAGAGCTGCCC130.8701472556894244No Hit
GTGGTGAAGGTTCTAAGACGTGGGATCGTTTCCAGATGAGAATTCACAAGCGACTCATTGACTTGCACAGT130.8701472556894244No Hit
GGTTTTAATGCTACATAAATATGCATTGTCTCATGCCTGTTTGTGTTGGATGCCTTGTGCTAAGCTGTAAC120.8032128514056224No Hit
TTACAGTGGTTTGCCATTAGGGTATTCATTCAGATAATGTTTTCCTACTAGGAATTACAAACTTTAAACAC110.7362784471218206No Hit
GAAATATATTTTTGGGTTACTTCAGTGTGTTTGACAAGACAGCTTGATATTTCTATCAAACAAATGACTTT110.7362784471218206No Hit
TGTGAAATAAAACTCAGTATTTTAATAAAGTAGCACGGTTTCTATTGAAAAAAAAAAAAAAAAAAAAAAAA110.7362784471218206No Hit
CCAATAAATGTTTGTTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.7362784471218206No Hit
ATCTAAAGCATTATTGCTTTAGAATGTTCAAATAAAATTTCCTGACCAAAAAAAAAAAAAAAAAAAAAAAA110.7362784471218206No Hit
GTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAA100.6693440428380187No Hit
GTTATTTTACTTGTATTTTTATTATCTGTTTGCCCCATAAATATTACTCTCATAAAAAAAAAAAAAAAAAA100.6693440428380187No Hit
GAAAAAGACAACCTCTACTTTTTTCTTTTGTATTTTGATAAACACTGAAGCTGGAGCTGTTAAATTTATCT100.6693440428380187No Hit
GCTTTAATTTTTTTAAATAAAAGAAATAAAGTTTATTCACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.6693440428380187No Hit
AGTGCAAGGAAAGTACATTTGTTTGCCTGTCTTTTCATTTTGTACATTCTTCCCATTCTGTATTCTTGTAC100.6693440428380187No Hit
ACACTGAGGAGGGCACATATTGCCGTGGTGTTGTAAAAAGGCACGACCTTCATTCTGATAATGAGAAAACA100.6693440428380187No Hit
ATATGATAATTTATAAAATATTTGAATAAAAAATGAATACTAGTTCCACATTTTAAAAAAAAAAAAAAAAA100.6693440428380187No Hit
ATCTTGTTAAATGCCAGTATCTCAGTCTTTCTGAAGCCCTGAAATGGTAATTGTAGCATTTCAGAAAATGT90.6024096385542169No Hit
GATAACTGAGCAAGGAAACGTAATTTGGATTATAAAATTCTTGCTTTAATAAAAATTCCTTAAACAGTGAA90.6024096385542169No Hit
CAATATAACTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.6024096385542169No Hit
TAGTAGTAGCGGTGGTCAGACATGGAAATGGTGGGGAGACAAAAATATACATGTGAAATAAAACTCAGTAT90.6024096385542169No Hit
AGACCATCCTGGCTAATACGGTGAAACCCCGTCTCTACTAAAAATGCAAAAAATTAGCCGGGCGTGGTGGC90.6024096385542169No Hit
GCCATTGAAAGTTAAAATGTTTGCATGGTTTACCCTCTGAGTTATGTTTCTTCTAGTGAGCATGCCTGCTG90.6024096385542169No Hit
CCATAATACATAACATTCAATTTTTACCACTTCTGTTTAGCGAACTTGTATACTTATTTTCTGTTCAGATT90.6024096385542169No Hit
GACTGGGTCCCAAGACAGCCACCAGGACAAGACACAAAGGCATTGTACTCAGCCCAATTCTTGCATGCCTT80.535475234270415No Hit
CAGAAAGACAGAGTGGAAACTAGCAGAGTTGTTCACATCATGGATTTTCAACGAGGGAAAAACTTGAGATA80.535475234270415No Hit
ATAAAATATATACTATATGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.535475234270415No Hit
CTAATACAAATACAAAAATTAGCCAGGCATGGTGGTCACTGTACTCCAGCCTGGGTGACAGAGCAAGACTC80.535475234270415No Hit
GCTGTAAAATGTTTAAAAACAAAACAAAACAAAAAAGAGGCACTAGTCTATCTGCAATTACTCAACGAGGC80.535475234270415No Hit
CACCACTTCAAAATATATTGTTGAAATTTTAAGAGGTTTTTATATAATCCTGAAGGCACCCACAAAGAAAA80.535475234270415No Hit
GTTTAAAAAATTCCCCCCATGTAATTATTGTGAACACCTTGCTTTGTGGTCACTGTAACATTTGGGGGGTG80.535475234270415No Hit
TTTCTATTTCTGTCAATAAAAGGAGATGAAAATAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.535475234270415No Hit
CTCTTACCGTATATGCGTAGTATTTGAGTTGCGAATAAATGTTCCATTTTTGTTTTCTAAAAAAAAAAAAA70.4685408299866131No Hit
GAATTTGACTGACATAGTTAAAAACTTAAGGTTTATTTTAGACTTTCAAGGAAACAAAAGACTTTAGAGTC70.4685408299866131No Hit
GTAATAAATTATTAAATTGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.4685408299866131No Hit
AGTTACAGGACATGATTAATGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCTGAATTTCTGACA70.4685408299866131No Hit
TTCCCTACACGACGCGGGGGAGAACGTAGAGTCTCACTCTAGATGTTTTATGTATTATATCTATAATATAA70.4685408299866131No Hit
GTGGGCATGTTATGGGGAGGGGAGGGGGTTCTTCCAGCCTCAGGTCCCAGCTGTCTCACGTTGTTTATTCT70.4685408299866131No Hit
CACCAATAAATGTTTGTTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.4685408299866131No Hit
GTCATTGTTTCTCTTCGATAAATTTATTTTCATTAAATACTTGTTAGAGGGTTTTGAAATGTTTTTCAAAT70.4685408299866131No Hit
AAATGAATGTTTACTGAGTACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.4685408299866131No Hit
ATGTTATCAGTCATAATTTTGGTTATTACATTAAAATGCTGCATGCCACAGAAATAACCAAGTTTCCTTGC70.4685408299866131No Hit
GAACAGTGTGGAGATGTTTTTGTCTTGTCCAAATAAAAGATTCACCAGTAAAAAAAAAAAAAAAAAAAAAA70.4685408299866131No Hit
AGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.4685408299866131No Hit
AGAAAAGAGGAGGAGAAAGAGGAAAGAAGAAGAAAAAAGAAAAAAGAAAAAAGAGAAGAAAAAGAGGAAAA70.4685408299866131No Hit
GTATTAAAGCTTAATGCCAGTCCTTATTTTTAATATTAACAAACTCTTATCATCATCTATATTTGAAATAA60.4016064257028112No Hit
CAATGATTGAAGCTCACGTTGATGTCAAGACTACCGATGGTTACTTGCTTCGTCTGTTCTGTGTTGGTTTT60.4016064257028112No Hit
TGTCTTAGCTCTGGACGTGAAATACTTCGATGATGATGATGATGATGATGATGATAATAATAATAATAATA60.4016064257028112No Hit
GACCAGTAGTTTAGTAAACCAGTTTTTTATTCATTTTAAATAGGTTTTAAACGACTTTTGTTTGCGGGGCT60.4016064257028112No Hit
ACCCTGGGTCAAAAAGAGAATAATTTGGCCTATAATGTTAAAAGAAAGCAGGAAGGTGGGTAAATAAAAAT60.4016064257028112No Hit
ATAAAAACTCAATATAACTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.4016064257028112No Hit
GAAGTGGCCTCATGGATGAATCCGGGGTATGAGCCCAGGAGAACGTGCTGCTTTTGGTAACTTATCCCTTT60.4016064257028112No Hit
GCCACTCCACCCAGTGGCAAATCCACATGCAGAAATCTCCACCAAGGTTCCAGCCTCCAAAGTGAAAGACG60.4016064257028112No Hit
AAAGAAAACTCTTCCTGAATTAAGAAAATAAAAATGGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.4016064257028112No Hit
AGCCTTTATTTTATGAGATATTTATGTAAAAATAATTTTCATGCATATTGTATTAAAGCTTAATGCCAGTC60.4016064257028112No Hit
GAGTAACCCATGGCCTGCACCCTCCTGCAGTGCAGTTGCTGAGGAACTGAGCAGACTCTCCAGCAGACTCT60.4016064257028112No Hit
GTTTGCATCCCCTTGCCCTCAATAAATAAAGTTCTTTTTTAGTTCCAAAAAAAAAAAAAAAAAAAAAAAAA60.4016064257028112No Hit
AACTAGCATTGTAAAATTATTTCATGATTAGAAATTACCTGTGGATATTTGTATAAAAGTGTGAAATAAAT50.33467202141900937No Hit
GCTTAAGAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.33467202141900937No Hit
GTTGATTCTACATATGCAAACCAATAAGTATTGAAATGATCACATGATTTTTGTTCTCAGTTCTATTAATG50.33467202141900937No Hit
CTTCACTATCTGTGGCATGACCAGCTGTATCTGAAAGAGAAAAGACATGAAATATAAACCAACCTCCTCAT50.33467202141900937No Hit
GACTTGACTATCACTAATCTGCTTGAGGGGGCCGTAGCTTTCATGCCTGAAGACATCACCAAGGGAACTCA50.33467202141900937No Hit
GTCATTTGTAATATGTTTTGTGAGAATCCTTGGGATTAAAGTTTTGGTTACAAATTGTCAAAAAAAAAAAA50.33467202141900937No Hit
GTTTAATAAAGGGAACTGACTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.33467202141900937No Hit
AGATTAATGGCTCTGTTGTAATATACTGGTGATGCATCTAATTCTCCACAAAGACCAATAAATTGAATGTT50.33467202141900937No Hit
CACAAACAAAAAATTCTTTTTCGTGAAGAACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2677376171352075No Hit
GATGAATGGAATATGTAAGACTTGGCTCATAGAAACCTAATCAGATGGTTAGAGGTGTTGGCAGTTTAGGC40.2677376171352075No Hit
GATGAATGGAATATGTAAGACTTGGCTCATAGAAACCTAATCAGATGGTTAGAGGTGTTGGCAGTTTAGGA40.2677376171352075No Hit
ACTTAAATCCGTCCTGTTTAGCGACTTCAGTCAACTACAGCTGAGTCCATAGGCCAGAAAGACAATAAATT40.2677376171352075No Hit
CGCTGATCGTCAGATCAAATAAAGTTATAAAATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2677376171352075No Hit
CCTCTAGTGTCTCTCTGTATAGTGGGTCAGTATTACCTCTCATGAGAAAGTCCCAAACATACTGTCTATAA40.2677376171352075No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTT40.2677376171352075No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT40.2677376171352075No Hit
GCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGC40.2677376171352075No Hit
GTATTATGAAGATGAATTTGAAGATGAAGAAATGCTGGATGAAGAAGGTAGAACCAGGTTAAAAAAAAAAA40.2677376171352075No Hit
CTATAAAACGGCCCCACCCTTATCTCCCTTTGCTGACTCTCTTTTCGGACTCAGCCCGCCTGCACCCAGGT40.2677376171352075No Hit
CTTCAGTGGCACAGTGGAGCAGCTCTGAAGATGCAAAGATACACGAAAAAACTTCCAGAACATCTGGGAGA30.2008032128514056No Hit
GCCCAGGACGGTCTGTGCCCGAGGTTCCTGTCAATACAGGTTTTATTTTATCACTTAAAAAAAAAAAAAAA30.2008032128514056No Hit
CCTCTGTCTTCTGTTAAGTACAGTTGATACTGACATTGTTCACTCATCAAACCACATCTTGATGCTAAGTA30.2008032128514056No Hit
GTGGTTAGGGGTGAAAGGCCAATCAAACTGGGAAATAGCTGGTTCTCCGCAAAAAAAAAAAAAAAAAAAAA30.2008032128514056No Hit
GTTAACGGGTGAATAATAAAAGTATATATATATATTATATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2008032128514056No Hit
CTGAAGAATCACAAAAGAAGTGAAAAGGTCCTGCCCCGCCTTAACTGATGACATTCCACCATTGTGATGTG30.2008032128514056No Hit
TTCCCTACACGACGCGGGCTCCGATGACTTGTGGTTAGGGGTGAAAGGCCAATCAAACTGGGAAATAGCTG30.2008032128514056No Hit
ATATTGAACTATATAGTACTCGATTTCTTAAATAAAGCTTAAGAAAGGAAAAAAAAAAAAAAAAAAAAAAA30.2008032128514056No Hit
GTTGAATGGCACATCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2008032128514056No Hit
ACATTAAAATGCTGCATGCCACAGAAATAACCAAGTTTCCTTGCCAACCGTGTCACGAATATAATAAACTC30.2008032128514056No Hit
GGCATGAGTTACAGTGCTGTTGGCCATGAGCTCAATGTTAACGAGTCGACAATCCGGTACATCAAGAAAAA30.2008032128514056No Hit
GTTGATTCTACATATGCAAACCAATAAGTATTGAAATGATCACATGATTTTTGTTCTCAGTTCTATTAATA20.13386880856760375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13386880856760375No Hit
GTAGTACTGTTGCTGAATAATACTAGGTTTTTATGGAATAGGATGAATGCTTTTGAAGTATTAGGGCTTCA20.13386880856760375No Hit
TTCCCTACACGACGCGGGACACTCTTTCCCTACACTCTCTCCCCCGCATCTGAGGCAGATCGGAAGAGCGT20.13386880856760375No Hit
GTATTCTGCTGTTACTGTGTGGAATATTCTGTAAATGCCAAATAGATTCTTTTGGTTAAAGAAAAAAAAAA20.13386880856760375No Hit
GACTTCAATAAAGTCTATTTATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13386880856760375No Hit
GACCAGTAGTTTAGTAAACCAGTTTTTTATTCATTTTAAATAGGTTTTAAACGACTTTTGTTTGCGGTGCT20.13386880856760375No Hit
TCTCTAGCCTCTTTGTGTTTTTGTTGTTGTTGTTGTTGTTGTTTTATATAATGCATGTATTCACTAAAATA20.13386880856760375No Hit
GGACAGACAGTGATCTCGAAGAAAAAAGGGGGAGACCAAAGAATAAAAGAAGGGTTTCCCCACTAAAATGA20.13386880856760375No Hit
GACCAGTAGTTTAGTAAACCAGTTTTTTATTCATTTTAAATAGGTTTTAAACTACTTTTGTTTGCGGGGCT20.13386880856760375No Hit
TTCCCTACACGACGCGGGACTTAGTTGATGGGTTGATAGGTGCAGCAAACCATCCTGGCACATGTATACCT20.13386880856760375No Hit
GAGTAACCCATGGCCTGCACCCTCCTGCAGTGCAGTTGCTGAGGAACTGAGCAGACTCTCCAGCAGACTCA20.13386880856760375No Hit
CATTAAATGCAGAGATCTGCAAAAAGGGAAAATAAAAACACAAGAAGAAAAAAAAAAAAAAAAAAAAAAAA20.13386880856760375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)