FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12989
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5974.59619678189237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500.3849411040110863No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC260.20016937408576488No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG230.1770729078450997No Hit
CACTTGTAGCATGCTAGGGATTTGGAAAAATAGTTTGCCATTTGTCCAAATCCCTAGCATGCTACAAGTGA220.16937408576487797No Hit
TTGTTACTAATAGAAATACACTATTTTGCTTATCCATATTTTTTATTTCTCAACTTCTAATCACTCATGAA210.16167526368465623No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC200.15397644160443452No Hit
GATATGTATGTTGTGAATTATGGATCAGGGTTCTTTTTTTCCCCCATACAAGTATCCAGTCATTGTAACAC190.14627761952421278No Hit
CCATGATGTCTTCGTTAGAAAAGTAAAAATGCTGAAGAAGCCCAAGTTTGAATTGGGAAAGCTCATGGAGC190.14627761952421278No Hit
GTTATATCAGTGCAGCAGAACTACGTCACGTCATGACAAACTTAGGAGAAAAACTAACAGATGAAGAAGTA190.14627761952421278No Hit
TCTCTGATATGATTCGAATTTTGGTAACTTTTGAAAGTTATTTTCCCCCTTTAGTCATGGATTTCTATTTG180.13857879744399107No Hit
GTGTATGTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGT180.13857879744399107No Hit
CCAGTACAAGATCCCAGACTGGTTCTTGAACAGACAGAAGGATGTAAAGGATGGAAAATACAGCCAGGTCC180.13857879744399107No Hit
GTGACAAGACCTTGGGGCGTACCAACATTTAAAGGACTGGTCACTAAAGAGGAACTTACAAGGAGACTAAG170.13087997536376936No Hit
GAACTCAGAGGAGTCACTGTTCCTTTTTTTTAGGTTAGAAATTAACAGCAGGGAAATGCCATCTTATTACC170.13087997536376936No Hit
TTCCCACTCTTGACAGAGCCCCATTAAGGCATCTGGGTGGGTTTTAAACAGAATGCCTATGTAGCAGTGAT170.13087997536376936No Hit
AGTTAACGCATTTACTAAACGCAGACGAAAATGGAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGA170.13087997536376936No Hit
ATAAGATTCATATTTACTTCTTATACATTTGATAAAGTAAGGCATGGTTGTGGTTAATCTGGTTTATTTCT160.12318115328354762No Hit
ACATTTATCTTTATAGCTTTTCTGTACCTAATATTGTGTCTTGTGCGTAGGATGTGCTCAATAAAAATTGA160.12318115328354762No Hit
CATTGTTACAGGAGGCAGGCCAGGACATCAAAGTCATCATCTTTATGTGGCATGACTCTTAAGAGGCCATT160.12318115328354762No Hit
ATAATAAACTGTTAACTCTTTTCAGTATTTAATACCTGTAGTTCAGTTAGTAACTTTTTCATATATAGCAT160.12318115328354762No Hit
ACACTGGCAACACCTCTTAGTTGATTTCTGTTTTCTTCTCTTTTCACTTTTTGTTTCTACCAGGGTAGAGG160.12318115328354762No Hit
CCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCCAGCTAAGGTTGAAGCCAAGGAAG160.12318115328354762No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGC150.11548233120332589No Hit
GGTGGAGGGAGTAGGGAAGTGGTCCTTTTACAAGAATTTTGATGCATAAGTGTCTATTGTAGGGTTTGGAT150.11548233120332589No Hit
CACCAAATCAACCTAATTAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGCCTTTGTAAAATTGA150.11548233120332589No Hit
GAGCAGGGTTGGGGGAGAGGCTGAGGAGAGTGTGACATGTGGGGAGAGGACCAGCTGGGTGCTTGGGCATT150.11548233120332589No Hit
ATTATAAGGTGGATGAGAATGGCAAAATTAGTCGCCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCT150.11548233120332589No Hit
GGTGTATACTGCCACATCAGTGTTTGAGTCAGTCCCCAGAGGAGAGGGGAACCCTCCTCCATCTTTTTTGC150.11548233120332589No Hit
ATATTAGCCGGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCCGGAGGCTGAGGCAGGAGAATGGAGT150.11548233120332589No Hit
CACAAAAGTGTGTCTAGAATGAATCCAGCATGTGTAAGATACTAGGATATTTTTAGTCAGTTGTGTGTTGC150.11548233120332589No Hit
CATCTAAATATATCAGTAAAATAGAATGCCTTAATATTTAATAAATAACATGATCTGCCAGAAAATCATAT150.11548233120332589No Hit
CTGCTGATGTGTAACAAATCTTACTGTGCCGAGATCGCTCACAATGTTTCCTCCAAGAACCGCAAAGCCAT140.10778350912310417No Hit
CTCCACAGTGTTTATATTGGGAAGATATTGGGAAGGAAATATATTTGTAAAAGATGAAGGCTGTATCTATT140.10778350912310417No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC140.10778350912310417No Hit
GTATCAGGCTTGTTCTGTGCCTAGGCTTAATTACCAGATTTCTGTGCTTCTGGACTTATAATGTATAGTTG140.10778350912310417No Hit
TATCCTCTCCTTCCATGAGGAAAGCCGCCCTTTTGTGTGTGAACATGCTGGCTGTGGCAAAACATTTGCAA140.10778350912310417No Hit
ATACTTTCTTCACCAATCTCATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACGAG140.10778350912310417No Hit
GTACAGACTAGCACAGAAGTTGGGCTAATTAAACAAGTATTGCTGAAAATGAGTGCTGTAGATGACATGAG140.10778350912310417No Hit
GCCATATTTTAAATCCCTATGTCTAGGGTAGTTTAGGTGTTTTGTTAGGTATTTTTGACTTCCTGATCAAC140.10778350912310417No Hit
ACACATCAAACTCCGGGCCACAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCA140.10778350912310417No Hit
TTAGGACACTGTTCACATATCACAGGAGGATCACTTGAGCCTGGGTGTTCAAGATAGGCCTGGTCAACACA140.10778350912310417No Hit
ATAATACATCATATCTTAATTTCCACTGAACTTGATTTAAATTCAGAGTTACACAGTATGAATATCACAAT140.10778350912310417No Hit
ATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCAC140.10778350912310417No Hit
AACTGAGGGACATCTGTTGGTCTATGTATAACTTTTAAAATTGGTATAGTATCTACAGAGTGTATAATTTA140.10778350912310417No Hit
CTTGTCAGCCGGGTGGGTAGGAACCGTAGACTCGCTCATCTCGCCTGGGTTTGTCCGCATGTTGTAATCGT140.10778350912310417No Hit
AATTTTTACATTTGGATCTTACAGATTAGGAGTGCATACAAAAGGTGCTGATATTGATGCGTTGTGTGTTG130.10008468704288244No Hit
GAAGAAACTCGAGGAGCTAAAAGCGAAGGCCGCGGGGAAGGGGCCCTTGGCCACAGGTGGAATTAAGAAAT130.10008468704288244No Hit
GGGTTTATGGAGTTTAAGAAATTTATTTTCCAGGTGCAGCCCCTGTCTAACTGAAATTTCTCTTCACCTTG130.10008468704288244No Hit
TTCAAAGTGTAACACGCTGATTTCCTCAAATAGAGATACCCCTTTGAGTGATAAATTTGCAAAATGCTGTC130.10008468704288244No Hit
GTAGAAGGTGGAGATGCTGGCAACAGGGAGGACCAGATCAACAGGCTTATTAGAAGAATGAACTAAGGTGT130.10008468704288244No Hit
GCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAA130.10008468704288244No Hit
ATGCTGTTCTCTATCAGCCTCTATTTGATAAGCGATTTGAAATTATTAATGCAATTTATGAACCTACGGAA130.10008468704288244No Hit
TACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAAC130.10008468704288244No Hit

[OK]Adapter Content

Adapter graph