FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45121.989273525109702No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG321.5602145294978058No Hit
CCCATGCCCCATTACAGGCTCACACCCAGTCTGATCAGGCTGAGTTTTGTATGTATCTATCTGTTAATGCT170.8288639687957094No Hit
GCTCAGAAAAAAGGTACATCCAGCAGTGGTCATTCGACAACGAAAGTCATACCGTAGAAAAGATGGCGTGT150.7313505607020965No Hit
GGGCATGGCAGTGGCCTCAGCCTTAAACTTTTGTTCCTACTCCCACCCTCAGCGAACTGGGCAGCACGGGG110.5363237445148707No Hit
GCCTTCTGAGCCATGGCGTGGTGTGTTCATGCCAAGGGTATATTTTCAAGCACATTTTCAAAAACAGAAAA100.48756704046806437No Hit
TCACAAGGTCAGAAGATCGAGACCAGCCTGGCTAACACGGTGAAACCCTGTCTCTACTAAAAATACAAAAA90.43881033642125794No Hit
GAGGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGAT80.39005363237445145No Hit
CATTGCTTTTCACATCCATTTCCCCTCCTTCCCATGGGCAGAGGACCAGGCTGTAGGAAATCTAGTTATTT80.39005363237445145No Hit
ACAATGAAGGAAATCAAAGGCATCCAAATGGAAAATGAAAAATAAAATTATCATTGTTTGCAGATGATATG80.39005363237445145No Hit
ACGCCGGATTCCCCGGGGCCGGCGCATCTCCGTGATCTGCCGCTCCCTCCCTGAGGGCATGGGGCCAGGGG80.39005363237445145No Hit
ATATATTATTCATCTGGTTTTAATGCTACATAAATATGCATTGTCTCATGCCTGTTTGTGTTGGATGCCTT80.39005363237445145No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA70.3412969283276451No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT70.3412969283276451No Hit
GAGTTCTGGAGATGGCAGCTTATTGGACACATGGATTTTCTTCAGATTTGCACTTACTGCTAGCTCTGCTT70.3412969283276451No Hit
GCCCTGCTGCAAAGATGGTCAACGTACCTAAAACCCGAAGAACCTTCTGTAAGAAGTGTGGCAAGCATCAG70.3412969283276451No Hit
CCCATAAAGGGAACACATTTGACTTTTTTTTCTCATATTTTAAATTACTACAAGATTATTAAAGATAAAAT70.3412969283276451No Hit
ATTGAGCATTTTTTCAGTTTCACACTTGTTTTCCTTTTGTTAATTTGCTATATTTGTTAAATATAGCATAT70.3412969283276451No Hit
GAGCCTCTCAGCTTTTTCGTATTTGGAGATATAACCAAGAACTAAAAGCTAAAGCACACAAATAAAAGAGT70.3412969283276451No Hit
GTTTTGCCCTTAAGAAGTCATGATTAACTTATGAAAAAATTATTTGGGGACAGGAGTGTGATACCTTCCTT70.3412969283276451No Hit
ACATAAACTGTTATTTCACTTCTACAAATCAGCAAGATATTATTTATGGCAAGAAATATTCCATTGAAATA70.3412969283276451No Hit
TCCCTACACGACGCGGGACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.3412969283276451No Hit
CCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGTGCAAGGAGCTGG70.3412969283276451No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA60.29254022428083865No Hit
GTTCAGGAATATACAGAAGCTACCTGTGGAAAGTAAGAGTGAAGAAACAAATACAGAAATTGTTGAGTGCA60.29254022428083865No Hit
CCCTAAGTCAAGTCACGGGTTTCCATAGCCAGGCAGTTGGTATGTACAATTCAGTTCAGCGTATGAACTTG60.29254022428083865No Hit
CCGCCTGAGTCTCAGGAGAAGAAGCCGCTGAAGCCCTGCTGCGCTTGCCCGGAGACCAAGAAGGCGCGCGA60.29254022428083865No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.29254022428083865No Hit
GGCATTGCCCTCAACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAG60.29254022428083865No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.29254022428083865No Hit
CTATTGATGAGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.29254022428083865No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA50.24378352023403219No Hit
ATACTTCTAATTTGAGGACTGTATGCCTTGATCACAAGCATTAGTGTGTAAAAATATTGAATGGTAAGATT50.24378352023403219No Hit
CCATCTTGTAAATATTGTAATACTTTAATTTTTAATATTATAAGCTTACATTTGCTCTGAAGTAAATGACT50.24378352023403219No Hit
TCCCTACACGACGCGGGAACCAGCCTGGGCAATGTAGCAAGACGCTGTCTCTATTTATACAATAAAATTTT50.24378352023403219No Hit
ATGCAAACCATCCAAAAGACCACCACCCGCCGGATAGTGGATGGCAAAGTGGTGTCTGAGACCAATGACAC50.24378352023403219No Hit
CTCTCACTGCCCAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.24378352023403219No Hit
GGTTACAATTCTGGGAGCCTTACTCTAGAATGTTGGTCCACCCTGATTTCTATATCTTGGTCTCCATAACT50.24378352023403219No Hit
GATGTCCAGAATGACTGAAGAATTGTCAGGGAAGAGTGATGAGCTGATTCGATACCAAGAAGAGCTTTCCT50.24378352023403219No Hit
GTACCAAACCTTTATACACTGTTTTTTCCATATATATATACCTATGATAAAGTATAATGTATAAATTAAGC50.24378352023403219No Hit
TCCTAACACCATCACTTTGACAATCAGATTTCAACATATGAATTTGTGGGAGTCACCAGCATTGAGACTAT50.24378352023403219No Hit
CTGTTACTACTTTTTTAATACAAAAAGATAAAAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.24378352023403219No Hit
GGGCTGCCTGCCCATCTAGGTCCCCTCTCCTGCATCTGTCTCCCTTCATTGCTGTGTGACCTTGGGGAAAG50.24378352023403219No Hit
GCACTTCTCTTTCTGTGTCTGGGTCCTGCCAGGCTGGAACCTCTATGAGGACAGGGACTCCTTACAAAATT50.24378352023403219No Hit
ATTCCAGCTTGAGGTGAGTGGGCTACTAGGGGAGATGAACTGCCCGTATCTTTCACTGACATCTGGGGATG50.24378352023403219No Hit
ATGTATGTCAAGTTGGTGGAGGCCCTTTGTGCTGAACACCAAATCAACCTAATTAAGGTTGATGACAACAA50.24378352023403219No Hit
CCTCTTTTCTTTAAAATTTACATAATCACAAGAAAGGAAAGAATTACAGTTGGACTGATTGTGACAGTGCC40.19502681618722573No Hit
CTTCAGAGGCTCTTCAGGTTCTCCTCTGTCATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGG40.19502681618722573No Hit
CCTTGAGTCAGAGCTGGCACAGACCCGGGCAGAGGGACAGCGCCAGGCCCAGGAGTATGAGGCCCTGCTGA40.19502681618722573No Hit
CACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAAT40.19502681618722573No Hit
GGACAAGTGCAGCCTGTCAGTTTTAGAAGCAAGTAAATCATCCTCTTTTCAAGAGCAGTGTCCGTGGTGTG40.19502681618722573No Hit
ATTTAGGACAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19502681618722573No Hit
GATATAAATGGCTGGTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19502681618722573No Hit
ATACCCCCTTTCTCCATTAGTGGCATGCTCATTCAGCTCTTATCTTTATATTCCAGTAAGTTATTTTGCTC40.19502681618722573No Hit
GTATTCCATCCTGGGCAACAGAGCCAGACCCTGCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19502681618722573No Hit
ATATTAAGGCACATTTGAATAAATTCTATTACCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19502681618722573No Hit
TCATTGAAGAGTATTTCAAATGCAAGAAATGAAGAAATAAATCTTTGGCTCACAAAAAAAAAAAAAAAAAA40.19502681618722573No Hit
CCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTATTCACTATAACTTAACACTACGAGAGACT40.19502681618722573No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19502681618722573No Hit
GGAACGGAAAGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGGAATGGCATG40.19502681618722573No Hit
GTCGTGGCCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19502681618722573No Hit
AATATCACTCTCCTACTCACAGGACTCAACATACTAGTCACAGCCCTATACTCCCTCTACATATTTACCAC40.19502681618722573No Hit
ACTGTGATGACTGGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAG40.19502681618722573No Hit
CCTGTCCTGCCAGCTCTGCTGCCACCTCTGACAGGTGTGCCTGCCCCCATCTCTTCTGATTGCTGTTAACA40.19502681618722573No Hit
GTGACTTCAAGATCAAATGTGTGGCCTTTGACTGAAATCAGCCAGCCCATGGCCCCCAATAAAGGCAGCTG40.19502681618722573No Hit
CCCCTGGGCCGGTGGCTCTCGGGGGCTGGTCCATGGGCGGCTGTGGCGTCAGGATGGAGTCCTAGCTGTGA40.19502681618722573No Hit
CTTTTATACATTTGTAATAGATAAAAATAAACCAGATTGCAAATCCTTTTTTAAAATCCTAAACCATGTAC40.19502681618722573No Hit
TTACCGGAGAATCCCTATCACTCAAGACCAAGACCGGATCCCAGCCCTGAGATCGAGGGAGATCTGCAGCC40.19502681618722573No Hit
GGCCAAGGTGGGTGGACAGCTTGAGCTTAGGAGTTCAAGAGCAGCCTGGGCAACATAGTGAGACTTTGTCT30.14627011214041932No Hit
GGGACGTGAGGCTTCCGCGCCAGGAACCCCTGCGGGCTAAGACGGTGTTGGCCAATCACATCCAGTCACTC30.14627011214041932No Hit
TTCATGATCACCTTAATGCTCATGAGACTATATTTATGATCAGTGAATAAAAATGTCAGAACTGTGAAAAA30.14627011214041932No Hit
AGCTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGCGACTGAGCAAGATTCCATCCCATAAA30.14627011214041932No Hit
GGGTCTAGGAGGGGCATTTTGGAGACTGCCTGACACCACCCCTATCCCCTGCCTCCCCCTCTCAGAAGAGG30.14627011214041932No Hit
CTCGCTGCAGTTCCCTTTGGGTTCCATGTTTTCCTTGTTCCCTCCCATGCCTAGCTGGATTGCAGAGTTAA30.14627011214041932No Hit
GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGG30.14627011214041932No Hit
CTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATTACCTGAGGTCAGGAGTTCGAGACC30.14627011214041932No Hit
CCTGAGCAAGAAGGAGCTGAAGGAGCTGATCCAGAAGGAGCTCACCATTGGCTCGAAGCTGCAGGATGCTG30.14627011214041932No Hit
GTATATAGGCTACTGAAAGAAGGATTCTGCATTTCTATTCCCCTCAGCCTACCCACTGAAGTCTTTGGGTA30.14627011214041932No Hit
GTTTATGTTCTCTTACTATATTATTTCATGTATAATTCTCAAGCTTTTACTCTTGTTTTCCAATTACATGA30.14627011214041932No Hit
AAATAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC30.14627011214041932No Hit
CTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGAGGGCAGATCACGTGGTCAGGAGATCGAGACCAT30.14627011214041932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.14627011214041932No Hit
CATGAAAACTCATGCCCCAGAAAGGGATGTATGTCGCTGTCCAAGAGAAGGCTGTGGAAGAACCTATACAA30.14627011214041932No Hit
TTCCCTACACGACGCGGGGAGTGACGGTGGCGTTTCCTTGAGGAAGAGTGAGGGTTCCAACTTTTCTGCTT30.14627011214041932No Hit
GTTTCCTGAACACAGACCTCTTTCTTGTGCCTTGTTCACTTCTGTTGAACCCCTCGTACTGCCGGGCATTT30.14627011214041932No Hit
CTATTGGAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
TTCCCTACACGACGCGGGGTCTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
GATTTCAGTTCTGTGAAATATTTTGGGATCTATACCAATTAAACATTTTCATAGTTCTGCCTATTGTCCTT30.14627011214041932No Hit
CCTTCAGTTGATAATAAACCTTTCTGAGATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
AGGAAGCAGGGAAGAGTGGGGAGCCCTCAGAGAGGTGGCAGGCAAGCCAAGCTGGAAAGAGATGAGTGAGG30.14627011214041932No Hit
GTTCTAGGAAAATAAAACCCGTTGATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
TGACCACGTCCACCTTTAAACACGGGGCTTGCAACTTAGCTCACATCTGACCAATCAGAGAGCTCAGTAAA30.14627011214041932No Hit
CCTACAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCTCTTGAACCTGGAGATGGAGGTTGCAGTGA30.14627011214041932No Hit
CTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
GGCCTTCTGGCAAGCCCTGGATGAAAACATGGACCTCTTGGAAGGTATAACTGGCTTTGAAGACTCTGTCC30.14627011214041932No Hit
GAACTGGAATCAACTGCTTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
GTATGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14627011214041932No Hit
AGCCTGGGCAACACAGCAAGATCCTGTCCTGTGTCTTAAAAAAAAAAAAAGATTCAGAAATGACATAAATA30.14627011214041932No Hit

[OK]Adapter Content

Adapter graph