FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5555
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2284.104410441044105No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG2153.87038703870387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG611.098109810981098No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA601.08010801080108No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA490.8820882088208821No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT410.738073807380738No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC190.342034203420342No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC160.28802880288028804No Hit
CATTAGCCAGACTAATAAAGAACAAAAGAGAGAAGAATCAAATCGACGCAATAAAAAATGATAAGGGAGAT130.234023402340234No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC130.234023402340234No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTA120.21602160216021601No Hit
GGTAAAGATCTATTAATTATAGTGTCTAATACACCCTTGAGGAAAATCTGTTAACTCCAAATTATTAAGTA110.19801980198019803No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAA100.18001800180018002No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC100.18001800180018002No Hit
GACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCA100.18001800180018002No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC100.18001800180018002No Hit
GGTTTTTAATAACACTTCTTTTTTGCTGTAGTCAAGCATTAATAGAAACTAGTTTAATTTTCACAGTGGTA100.18001800180018002No Hit
GCTCCTGAAGGAAGCGCTAAACGTGGAAAGGAACAACTGGTACCAGCCGCTGCAAAATCATGCCAAAATGT100.18001800180018002No Hit
GAGAAAGCCAAGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGT90.16201620162016203No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG90.16201620162016203No Hit
GACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGT90.16201620162016203No Hit
CTCTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTG90.16201620162016203No Hit
CTTCCAATTTACATACTCTGCTTAGAATTTGGGGAAAAATTTAGAAATATAATTGACAGGATTATTGGAAA80.14401440144014402No Hit
GTTTAAAAATTATCCTATTAAATGTTGGTTAATAGTTGTGCAGTTTTTCATTTCAGATGGAAAGGCAATGC80.14401440144014402No Hit
CAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAAT80.14401440144014402No Hit
ACATTGATTTTGGAGTATTTATATTATTCAAAACTGCTGACCTTATGTCTTTTGTAAAACTTGAGCAATGA80.14401440144014402No Hit
GTTCCCATGCCTGGAGAAGCTAATGCCAACTCATCATGTGATAATTCAATTTGTACAATAAATTATGAACC80.14401440144014402No Hit
GGTTTGCAGAAGAGTTTGTGGGTCTAGTGTTCAGTGAAAATGAAAACTCTGCAGCTTTCTACGTGATGGGT70.126012601260126No Hit
CTTGGATTATGGATCTGAATAGAGAAATGCTTACAGATAATCATTAGCCCACATACCAGTAACTTAAAGAT70.126012601260126No Hit
CATTACATACTGAATTGGGTCCTTGTCATTTCTGTCCAATTGAATACTTTATTGTAACGATGATGGTTACC70.126012601260126No Hit
GGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCTTTTTG70.126012601260126No Hit
CGTTCCTTGTTGCCACTTTGGGTATTCTGAGATTTTCTCTTGCATGCCCTTAGCTTTACAGCTTTTGCATT70.126012601260126No Hit
GATGGCAAACATAGTGGCAAGACAACATTACCCAACTATAGAAGAGAGGCTAACTAGCAACAATAATAGAT70.126012601260126No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCC70.126012601260126No Hit
GGCTCTGGTGGGTGTTCCTTGTTTTAGGCTTTCTCCTGTTTCTCAGAGGATTTATCAATTATGCAAAAGTT70.126012601260126No Hit
GTAGAATGTGCATGTTGTAGTCCTATGATGATGTAAACTTGGTACTACATAATGACTTGCTCCACACATGC70.126012601260126No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT70.126012601260126No Hit
GAAGAACAAAAAACAGCGCATGATGGTTCGAGCTGCAGTTTTAAAGATGAAGGATCCAAAACAGATAATCC70.126012601260126No Hit
GGATGGACGCTAAAATATATTTGATAAAGCGCAACATAATGAATAAATAAAAGCACTTCGCTGCAAGTGAC70.126012601260126No Hit
CCTCACAGTTGCCATGTAGACCCCTTGAAGAGGGGAGGGGCCTAGGGAGCCGCACCTTGTCATGTACCATC70.126012601260126No Hit
CTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTT70.126012601260126No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAG60.10801080108010801No Hit
AGTGCTACAATTAGATTCAACAAGAGAGAAGAGTGCTGTGATGGGAAAACTTTACATTCTCAGACATTATT60.10801080108010801No Hit
GATGGGCCTCATAGTAAAGTTATACAAATGGATGTGGCTTTGTTTGAGATGAATCAGTCGGATTCAAAAGA60.10801080108010801No Hit
ACAACAGTTAAAAACAAGGATATCAGGAAATTTTTGGATGGTATCTATGTCTCTGAAAAAGGAACTGTTCA60.10801080108010801No Hit
AGCTAATGATCAGAGAGATTTTTTTTTTTAAACTACCATGGTCCCAGGATTCCATCCTGAAATTTATTTTT60.10801080108010801No Hit
GTTGAGCCTTTTTGGCCTGGCTTGTTTGCAAAGGCCCTGGCCAACGTCAACATTGGGAGCCTCATCTGCAA60.10801080108010801No Hit
GTTATCCCAAAGCCGTGTGTTTGTGATGTTTTGGAGTACATATATATGAAAATTATTATGACACGCACTTT60.10801080108010801No Hit
GTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACC60.10801080108010801No Hit
CTTTGACTTCTTGCATGCGAGGGACCATTGCGTGGCCCACAAACTCTTTAACAACTTGAAATAAATGTGTG60.10801080108010801No Hit
GACTCTGATCTTCCAAGTGATTTCAGCATCCATGAGTTTTTGGCCACGTGCCAAGATTATCCGTATGTCAT60.10801080108010801No Hit
GTGTTAAGTGAAAGAAAAAGTCGAAAGTCTCTCGCTTTAAATCAAAAGCTAGAAATGATTATGCTTAGTGA60.10801080108010801No Hit
GATTAAGAGTGTTGGGGGGGCCTGTGTCCTGGTGGCTTGAAGCCACATGGAGGGAGTTTCATTAAATGCTA60.10801080108010801No Hit
ATTTAAGAGAACAAGCAAATATAAACAGCAACTTGTGTTCCGAACAGGAATTTTCATTGTTCTATAACAAC60.10801080108010801No Hit
GCTTTACTTCCAACTATGTGGTCAATTTTGGAATAGGTGTGGTGTGGTGCTGAAAAAAGTGTATATTCTGT60.10801080108010801No Hit
GTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAAAGACTGGTTAATGATAACAATGCA60.10801080108010801No Hit
CCTGCGAGTCACTCCATTTATCTTAAAGAAATTGGACAGCATATGAAGACAGGACATCACATATGAATGCA60.10801080108010801No Hit
GTGTAGCCTAAAGGGCAGGGCCCACGTGTATAGTATCTGTATATAAGTTGCTGTGTGTCTGTCCTGATTTC60.10801080108010801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)