FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4938
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1583.19967598217902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA601.2150668286755772No Hit
ATAGACTGTGTTGCAGTACACAAGTTGCCATAATAGTATAAAACAGTAAAATGTGCTTAAAAGGCCATCCT170.3442689347914135No Hit
ATACAAAGCAACTACACCTGGATTTTTCCAAACAACTTTTATTTCCTCAGAGTCTTCCTTAATCCTATGGA140.28351559335763465No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC130.26326447954637505No Hit
GCCTGCTTCTGCAAAGTGGTACGATCGAAGGGACTATGTCTTCATTGAATTTTGTGTTGAAGACAGTAAGG130.26326447954637505No Hit
TATCGGTGCTTTGCAGGAGGCAAGTGAGGCCTATCTGGTTGGCCTTTTTGAAGACACCAACCTGTGTGCTA110.22276225192385582No Hit
ATGTATATGTGTATATAAGGTAGTATTCAGTGAATACTTGAGAAATGTACAAATCCTTCATCCATACCTGT110.22276225192385582No Hit
ATTGTATGATTACGAATAGTCTGTATGTTTCAAGCCCTTCTGTAAAATATGAAGAAAAGTGCTCTTAGCAT100.2025111381125962No Hit
GCCTTGGCCCTTTCCTGTGGAACAGAAACAATTTCACAGAACATCAGCAGCAGACAAGGCCACTCTGCAGC100.2025111381125962No Hit
GCTGTGGAATGCCCAGCCAGTTAAGCACAAAGGAAAACATTTCAATAAAGGATCATTTGACAACTGATAAA100.2025111381125962No Hit
GATTTCAACATGAGATTTGGAGAAGTCAAATATCCAAACCATAGCACACACCACACATAAAAACTAACTCG90.18226002430133656No Hit
GGGGAGGCCGAGGCAGGAGGATCACCCGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCC90.18226002430133656No Hit
ATGAAAAACTTAAAGAAAGCAATACAACAAAGGGTGAACTTCAGTTAAAATTGGATGAACTTCAAGCTTCT90.18226002430133656No Hit
ATACAGTATAACAACTATTTACATAGTGCTTACATTGTATTAGGTGTTATAAGCAATCTAGAGATGATTTA90.18226002430133656No Hit
GGAAGAAGGAGCTTAATGCCAGGAACAGATTTTGCAGTTGGTGGGGTCTCAATAAAAGTTATTTTCCACTG90.18226002430133656No Hit
GGATGAGGGAGAAGAATAAAGCAGCTGCCTGGAGCCTATTCACTATGTTTATTGCAAAATCCTTTCGAAAT80.16200891049007696No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG80.16200891049007696No Hit
CCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAG80.16200891049007696No Hit
GAAAAACTCTAGCCAACAAGACAACTCTTCTCGGGAAAGTCCCAGCTTAGAGGATGAGGAGACTAAAAAAG80.16200891049007696No Hit
ATCTCCAGCAAGCATCTCATTTAAATAAAGGTTTGTCATCTTTAAAAATACAGCAATATGTGACTTTTTAA80.16200891049007696No Hit
CCAATAAATTCTACTTCCTGTCCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16200891049007696No Hit
ATCCTGGCCTGTCCATTGGTGATGTTGCGAAGAAACTGGGAGAGATGTGGAATAACACTGCTGCAGATGAC80.16200891049007696No Hit
ACTTGCTGCTGATGAAGATGATGACGATGATGATGAAGAGGATGATGATGAAGATGATGATGATGATGATT70.14175779667881733No Hit
GTATAAACTTACATCACGACTTTCTCTTAACAGCTGTTGTAAAGTTTATTACTTTATGTACAACTGAAGTT70.14175779667881733No Hit
ATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCT70.14175779667881733No Hit
GGCATGTTGTATTATATAACATATCTTGAGCATTCTTGACAGACTCCTGGCTGTCAGTTTCTCAGTGGCAA70.14175779667881733No Hit
GTGTGTGACTGTGGGAAGATGGAGGAGTATGAGAAGTTCTGTGAAAAAAGTCTTGCCAGAATACAAGAAGC70.14175779667881733No Hit
ACTGGTGTCTGAGTGCTCCGCGCGGCTGCTGCAGCAGGAAGAAGAGATTAAATCTCTGACTGCTGAAATTG70.14175779667881733No Hit
GTAGTGTACATTACCATATAATGTAAAAAGATCTACATACAAACAATGCAACCAACTATCCAAGTGTTATA70.14175779667881733No Hit
CTACTTAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAGCCGAGATCACG60.12150668286755771No Hit
GGTTAAAAGGCAACCTATTGTTGACTATGCTCACTACCCATGTGATGGGATAATTCATACCCCAAGACTCA60.12150668286755771No Hit
CCTTCGGAAAGAGAGAGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTGGCAGCT60.12150668286755771No Hit
ATAAGAAGGAGATTGAGCGTCTGCAGAAAGAAATTGAGCGCCATAAGCAGAAGATCAAAATGCTAAAACAT60.12150668286755771No Hit
CCAGTGCTCGAGACAACACCATCAACCTGATCCACACGTTCCGGGACTACCTGCACTACCACATCAAGTGC60.12150668286755771No Hit
CTCTCAAGCACGACTAAGAGCCTTCTGAGCCCAGCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTT60.12150668286755771No Hit
GTTTGGAGGGACAAAGCTAATTGATCTGTCACACTCAGGCTCATGGGCAGGCACAAGAAGGGGAATAAAGG60.12150668286755771No Hit
GATTCAGCTTACCAAATAAATACTGCCTAACACTGCAGAGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12150668286755771No Hit
GTCCTGCGCCGTCAGCACAAGCCACGCTTCACCACCAAGAGGCCCAACACCTTCTTCTAGGTGCAGGGCCC60.12150668286755771No Hit
ATTATAAGCAGCTGTATCCCCTTTGCCATGGTGATAGCAGACCATTCACATGGGAGCTTCTGGTCTCTTTG60.12150668286755771No Hit
CTTTGAGAGCGAGGATCAACTTCAGGATCCAGATGACCTAGATGACAGTCATAGGCCAAGTGTCTGTAGTA60.12150668286755771No Hit
GTGAAACAATGTTGTCCAACAATAAACAGGAATTTTATTTTGCTGAGTTGTTCTAACAAAAAAAAAAAAAA60.12150668286755771No Hit
GGAAAGCCTGATGCAGCAAAAAAGGGAGTTGTCAAGGCTGAAAAAAGCAAGAAAAAGAAGGAAGAGGAGGA60.12150668286755771No Hit
CACAACCACACACACAAATGGGGGCAACCACATCCAGTACAAGCTTTTACAAATGTTATTAGTGTCCTTTT60.12150668286755771No Hit
ATCCAGTGGTGGGTAATTGTACAATAAATTTTTTTTGGTCAAATTTAAAAAAAAAAAAAAAAAAAAAAAAA60.12150668286755771No Hit
ATATGATGCTGTGGCTGAGAAAGCTGGTAAAGTAGAAGCTGAGGTTAAACGCTTACACAATACCATCGTAG60.12150668286755771No Hit
GATATAATCTCATATTTGGAAAAACCTAAAGACTCGGCTAAAAAACGATTAGAACTGATAAACAAATTCAG50.1012555690562981No Hit
TCTCTAAGGAATCAATATTTCAATATGTTGAGTGTGTGTCCAATGCTATGAAATTAAAATATTAAATAAAA50.1012555690562981No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTT50.1012555690562981No Hit
ACCTACGCACTCAGTTGACTACGTAAATCTGTCAGGTCTTTTGTGATTTCCTCTGATGCAGGAGAGGAAAA50.1012555690562981No Hit
AAGTGAATTTGAATGAAAAATTTAAGCATTGTTTGCTTATTGTTCCAAGACATTGTCAATAAAAGCATTTA50.1012555690562981No Hit
GCACATCGGCAAGCCCTCCACCATCCGCAGAGATGCCCCTGCTGGCCGCAAAGTGGGTCTCATTGCTGCCC50.1012555690562981No Hit
GCCACTAATAGTTATGTCATCCCTCTTATTAATCATCATCCTAGCCCTAAGTCTGGCCTATGAGTGACTAC50.1012555690562981No Hit
GGTAATACGGACACAGTGACCCATCCTCCATCCTATTTATAGTGGAAGGGCCTTCAATTGTATCAGTACTT50.1012555690562981No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCAAAAAAAAAAAAAAAAAAAAAA50.1012555690562981No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA50.1012555690562981No Hit
GCCCCAAACCTTTTGTTCTCTCCGTGAAACTTACCTTTCCCTTTTTCTTTCTCTTTTTTTTTTTTTTTATT50.1012555690562981No Hit
TTGCTAAGATGGGAGGGCTGTTCTTAAATCACTCGTTCTTGAAGCTGCGAAAAAAAAAAAAAAAAAAAAAA50.1012555690562981No Hit
GGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCAGCATGTCATGCTCCCAGAATTTCAGCTTCAGC50.1012555690562981No Hit
TCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCG50.1012555690562981No Hit
CATTTTGAAGTTTATGAACGGTACGACAGTGTATTGTAAATTCCCTTTAGTTTGTTACAGTAGCCAATTGA50.1012555690562981No Hit
GGAGGAGGAAGAAGAAGGTGATGGTGAGGAAGAGGATGGAGATGAAGATGAGGAAGCTGAGTCAGCTACGG50.1012555690562981No Hit
AGTAAATAAACTGTTTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1012555690562981No Hit
GTGGAGCGGACCCACTCCCCTCACATTCCACAGGCCCATGGACTCACTTTTGTAACAAACTCCTACCAACA50.1012555690562981No Hit
ACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCAT50.1012555690562981No Hit
GGAGAGCTCCCTCCTAGAAAAGATGATAGTGTCGAACCAAGTGGAACAAAGAAAGAAGATCTGAATGACAA50.1012555690562981No Hit
ATCCCACCTTCAACACCTTACAAGTAAAGACAATGAAGAACAGTTGAAACATGCAAAATATGGAGCTTTTC50.1012555690562981No Hit
CCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAAC50.1012555690562981No Hit
GGACTGCATGCTACTGTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCCGATCAC50.1012555690562981No Hit
ATTTAGAGATGGTGACATTCTTGGAAAGTACGTAGACTGAAATAAGTCACTATTGAAATGGCATCAACATG50.1012555690562981No Hit
GAGAAATATTATGATGTTTATGTAATAAAGACATGTAACTGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAA50.1012555690562981No Hit
GTGTAACAACCATATAATAAATCACCTCTTCCGCTGTTTTAGCTGAAGAATTAAAAAAAAAAAAAAAAAAA50.1012555690562981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)