FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6546
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68610.479682248701497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65910.067216620837153No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.5041246562786434No Hit
GTAAGAGCAAAAGAACGTTTTTGTACAATTTTTTTTCATTTAATTGGAATGATCTTCAGGTTTCTACAAAT180.27497708524289644No Hit
GAATTAAACTTTTAATTCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.229147571035747No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGT130.1985945615643141No Hit
CTACTAAAAATACAAAAAATTAGCCGGGCGTGGTGGCGCCCGCCTGTAGTCCCAGCTACTTCCGGGAGGCT130.1985945615643141No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.18331805682859761No Hit
GTGGCATGGTTCGTGCCAAATTCCGAAGCAATCTTCCTGCTAAGGCCATTGGACACAGAATCCGAGTGATG120.18331805682859761No Hit
TTCCCTACACGACGCGGGACATACATGAAGATAGGTTGATTGAGATGATTCAGTATGAGAAAGAGAAAGGA110.16804155209288116No Hit
CTATTATGATGCTATAGTGAAGATTAAACTAGTTAATATAGAATATAAATACTTGGAAAGGCATCTGGCAC110.16804155209288116No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATT100.15276504735716467No Hit
GGTGGCGGGCGCCCGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGG100.15276504735716467No Hit
GCTCGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGGAACAAGGACCAGGAGGTG90.13748854262144822No Hit
TCTTTTACCCTGGCCCCTCTCCCTTGGGCTCTGATGAAAAATTGCTGACTGTAGCTTTGGAAGTTTAGCTC90.13748854262144822No Hit
GTCCTTGGACAGAGCCATGCGGCTGGTGAAAGATGTCTTCATTTCTGCGGCTGAGAGAGATGTGTACACTG80.12221203788573175No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGTCCATCGTCCTAGAATTAATTCCCCTAAAAATCT80.12221203788573175No Hit
GTCATAGAGTAGATGCTAGTCATTTAGGGCGAAGTTAGTAGTTGAAGTTTATTTGTTGGCTCATGTCAGTA70.10693553315001528No Hit
GCCTACCTGCAGAGCCTGGCCAAGAAGATCTGCTCCCATTCGGCCCCGGAACAGCAGGCGCGCACGCGGGC70.10693553315001528No Hit
TTCCCTACACGACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCT70.10693553315001528No Hit
GCTGTGCACTGCGCGGGCCTGAGACTGCTGGATACAATGTTGGGCAACAACTCAGCCAGCCTGATGGCAGC70.10693553315001528No Hit
GTGTCCAAATAAAAAGCGGTGGGCAAGGGCTGGCCGCAGCTCCTGTGCCCTGTCAGGACGACTGAGGGCTC70.10693553315001528No Hit
GGGAAGAGCACTGGTGTGGGGGTTCCACCGAGAAGGGGACCTCATCTAGAAAAGAGGTTACAAACCTACCA70.10693553315001528No Hit
CACAGACACCTTGGAAGAGCTTAAGGAAATAGATAAAGGCATGTGGAAGAAACTGCAGGAGAAGTTTGCCC70.10693553315001528No Hit
ATTAAAAGTAGCTTTGTAATTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.10693553315001528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)