FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418
Sequences flagged as poor quality0
Sequence length71
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29821.015514809590975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151.0578279266572637No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG70.4936530324400564No Hit
ATCTAGAAGATAATGATGTCTCAGACATTTTAAAATTACTAGCATTACAGGCTGAAGAAAATCTTGGTATG70.4936530324400564No Hit
CCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGGAGAACCTGAA70.4936530324400564No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT70.4936530324400564No Hit
GGGCAGGGGAATCACTTGAACCCAGGAGACAGAGGTTGTAGTGAGCCAAGATCACGCCACTGCACTCCAGC70.4936530324400564No Hit
GGCCTCCAAGGAGACTCATGTAATGGATTACCGGGCCTTGGTGCATGAGCGAGATGAGGCAGCCTATGGGG60.4231311706629055No Hit
CATCTGAACAAATCAATGTCTGGGCGGTGAGGCAGCTGCTTTCTCCTTCACTTCTTTGGGTTACTAGAGCA60.4231311706629055No Hit
GGGCACGTCCGTGGTGCTAATCTGCCCGCAGGACGGCATGGAGGCCATCCCCAACCCCTTCATCCAGCAGC60.4231311706629055No Hit
GAGACAGGAGAATCACTTGAATCTGGGAGGCAGAGGTTGCTGTGAGCCAAGATAGCGCCATTGCACCCAGC50.3526093088857546No Hit
GAGATTACAGGCGTGAGCCACCGCTCCCTGCCCAACACATATACCATCTGAAAATGTTAGAATTCTGAGTT50.3526093088857546No Hit
CTGATCACCTGTTAGCATTGCCATTCCTCTACTGCAATGTAAATAGTATAAAGCTATGTATATAAAGCCTT50.3526093088857546No Hit
AGCGTTTTCCATGTTGGAGGTCAAATCCCACGAGGTTCACGTATCTATTGCTGCTGCTTTACCCTGTTCTG50.3526093088857546No Hit
CCACTGAATGGGTAGGAGCAACCACTGACTGGTCTTAAGCTGTTCTTGCATAGGCTCTTAAGCAGCATGGA50.3526093088857546No Hit
GGGTAGCTGAGTCTGCTTAGGGGACTGCATGGGAAGCACGGAATATAGGGTTAGATGTGTGTTATCTGTAA50.3526093088857546No Hit
GCTAAAATATATAATATACTAAACCAACTCTAATATTGCTTCTTGTGTTTTACTGTCAGATTAAATTACAG50.3526093088857546No Hit
GGCTAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAACATTAGCCGGGCATGGTGGCGGGTGCCAGA40.2820874471086037No Hit
GGCGGGCGCCTGTAATCCCAGCTACTTGGGAGGCTAAGGCAGGAGAATCTCTTGAACCTGGGAGGTGGAAG40.2820874471086037No Hit
GGTGGGAAGATCACTTGAACCTGGGAGGTCAAGGCTACAGTGAGCCATGATGCACCACTGCACTCCAGCCG40.2820874471086037No Hit
GGGTGTGTCCAAGAAGAAATAAGTCTGTAGGCCTTGTCTGTTAATAAATAGTTTATATACCCAAAAAAAAA40.2820874471086037No Hit
GTACATGTGTGAAAACAATATTGTATACTACCATAGTGAGCCATGATTTTCTAAAAAAAAAAATAAATGTT40.2820874471086037No Hit
GACTCAGGTATACAACCAGGATTCTCAAGATTCCATTGGAGAAGGAGTTACCCAGGGAGATTATACACCTA40.2820874471086037No Hit
GAACACACCAGAAATTTGTCATTGCCACTTCAACCAAAATCGATATCAGCAATGTAAAAATCCCAAAACAT40.2820874471086037No Hit
GGATCTGAGTTGCAGCTCTGCACCCTAAATCACACCCTGGGCATTGTCTGGGCTGCAGGGCTGCCAGGTTC40.2820874471086037No Hit
GATCACTTGAGGTCAGGAGTTTGAGACCACCCTGGCCAACATGGAAAAACCCCCATCTCTACTAAAAATAC40.2820874471086037No Hit
CAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGT40.2820874471086037No Hit
AGTACCATTTACACCCACGATGAAGGGCTTAAATAGAAAATAATGGAAAATTAAATATTACTTTAAGGAAA40.2820874471086037No Hit
GTGTTATAATTTGCTTCATTTCCTTGCTATTTGATACATAGTGTGCATTTCTCTGTCACTGTAACTATTGT40.2820874471086037No Hit
GCTCTTAGGGAGGCTGACGCAGGAGAATGGCGTGAACTCAGGAGGCGGAGCTTGCAGTGAGCCGAGACCGC30.21156558533145275No Hit
GAGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21156558533145275No Hit
GTAATGTCTAGGCTTATTGAATAAAAGAATGTTCCCAAAATACCATGTGAAAGGATGTACTACTGCTACAT30.21156558533145275No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC30.21156558533145275No Hit
GTGGTGGCGCACCCCTATAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGC30.21156558533145275No Hit
TCCCTACACGACGCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21156558533145275No Hit
GGTGGGAGGATCACATGAGGCCAGGAGTTTGAAATCAGCCTGGGCAACAGAGTGAGACCTCGTCACTACAA30.21156558533145275No Hit
TGATCAACTAATAGCTCTGCTAGTAATTGATTTATTTTTCTTCAATAAAGTTGCATAAACCAAAAAAAAAA30.21156558533145275No Hit
GAGGTTATTGAATTCATTGGAGAATGAATGCTGTTCTGAATTTGTATCTTAAGGGAAATACTACAAAATGT30.21156558533145275No Hit
CCCTACACGACGCGGGAGCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21156558533145275No Hit
CAATAAAGATCGCCCCCACCTCCACCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21156558533145275No Hit
ACACGACGCGGGCACCTAGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21156558533145275No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21156558533145275No Hit
GTTAGACCTGTGTGTGTGTTTAAAAAAAGAGAAGTCAGTGCTCACTTTTTGTATTTAAATATTAAAAATGA30.21156558533145275No Hit
ACTCGGGAGGCTGAGGCAGGAGAATCTCTTGAACCCGGGAGGTGGAGGTTGCGGTGAACCGAGATCATGCC30.21156558533145275No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTGAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC30.21156558533145275No Hit
GAAGCCAAGGGGGGTGGATCAACGGAGGTCAGGAGGTGGAGACCAGCCTGACCAACATGGAGAAACCCCAT30.21156558533145275No Hit
ACACGACGCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACAC30.21156558533145275No Hit
TGGTGAAACCTCGTCTCTACTAAAAACACAAAAATTAGCCAGACATGGTGGTGCACGCCTGTAATTCCAGC30.21156558533145275No Hit
GAGTCAGCTAATTATGGATATTGTCATCTCGGTAACTTTGTAAAAAACAAACAAAACTGGCATTGTTAATT30.21156558533145275No Hit
CATTTGTAGGTAGTGAGACAAAATTGATGATCCATTAAGTAAACAATAAAAGTGTCCATTGAAAAAAAAAA30.21156558533145275No Hit
CCTTGGAAAAGGTGTGTGCTGACTTGATAAGAGGCGCAAAAGAAAAGAATCTCAAAGTGAAAGGACCAGTT30.21156558533145275No Hit
GCGGGGCCCAACATTGGTTATGGAAGCAACAAAAAAACAAAGCACATGCTGCCCAGTGGCTTCCGGAAGTT30.21156558533145275No Hit
GAACTTACTAAAGGCACTTCCTTCCTGTTAAACCCCTGTTAACTCTCCATAAATTTGGTGATTCTCTGCTA30.21156558533145275No Hit
GCTGAGGCAGGAGAATCACTTGAACCCAGGAGACAGAGGCTGCAGTGAGCCGAGATGGTGGCATTGCACTC30.21156558533145275No Hit
CCCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAGGCACTGTTCATCTC30.21156558533145275No Hit
GTGCAAGCACTTGGGGACAGCATGAGCTTGCTGTTGTACACAGGGTATTTCTAGAAGCAGAAATAGACTGG30.21156558533145275No Hit
GGAGGCAGCCCTGGTGGACATGGTGAATGACGGCGTGGAGGACCTCCGCTGCAAATACGTCTCCCTCATCT30.21156558533145275No Hit
ATCTGCAATGTAGGGGCCGGTGGACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGGAGGTCCTGCCCCCTC30.21156558533145275No Hit
CAATTGTCCATCTTTATTTTAGAGTTTTAATGAATTCAAGGAAGGGAGCATAGCATATCTGTGGCAAACTA30.21156558533145275No Hit
TTTACTATTCCAGGGTGACATAATGCATTTAAATTTGGGATTTGGGTGGAGTATTATGTTTAACTGGAGTT30.21156558533145275No Hit
TCCCTGCACTCCCCTCTTGCTGCGTGTTGATTTGGAGGCACTGCAGTCCAGGCCGAGTCCTCAGTGCGGGG20.14104372355430184No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAA20.14104372355430184No Hit
CATTTAAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GTGACCTTTTCTTCATTTTGGTTTATTTTTGTAATGTGAAAAGTATCACTCTAAAAAACATTTTTAATTTA20.14104372355430184No Hit
GCTATATACAGTGTAGAATAAATGTGGTAGAAAAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
CGACTAAGAGCCTTCTGAGCCCAGCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGC20.14104372355430184No Hit
GGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GAAATATAAAGCCCTGCAACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
CGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAGGAAAAGACTCATATCAACATTGTCGTCATTGGACAC20.14104372355430184No Hit
GTTTTTCCACTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
TTCCCTACACGACGCGGGACAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
ACAGAATCGCCGGACAGGTGGCAGCTGCCAACAAGAAGCATTAGAACAAACCATGCTGGGTTAATAAATTG20.14104372355430184No Hit
GAACTTGATCCTATACAGAAACTCTTTGTGGACAAGATTAGAGAATACAAATCTAAGCGACAGACATCTGG20.14104372355430184No Hit
ACTCATTGGTATGAAAATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
CTTCCAGACCATGGAGGAGAAGAAAGCATTCATGGGACCACTGAAGAAAGACCGAATTGCAAAGGAAGAAG20.14104372355430184No Hit
ACTTAGGAGGTTGAGGCTGGGAGGATCACTTGAGCCCAGGAGTCTGAGGCTTTAGTGATCTAAGATCATGC20.14104372355430184No Hit
GGGACAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
ACATATTACGTATAAATTTTGAAGAATTGGAATAAAATTGATTCATTTTAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
TACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCAAGACCCACGGA20.14104372355430184No Hit
GAGATTACAGGCGTGCGCCACCGCTCCCTGCCCAACACATATACCATCTGAAAATGTTAGAATTCTGAGTT20.14104372355430184No Hit
GACCTGACCAATGATTGGCATGACTCCCAGGAGAAGCCAGCGATGGAGAAATGGAGAGCGCAGGACTTCTC20.14104372355430184No Hit
GTCCCAGAGTCGCCAACGCATTGTCTGCCTCAACTCCCTCAAGGCTAGTGTGCAGGTGACCACCATTGACC20.14104372355430184No Hit
GTGCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GCTGCTGCTGCTAAAGTTCCAGCAAAAAAGATCACCGCCGCGAGTAAAAAGGCTCCAGCCCAGAAGGTTCC20.14104372355430184No Hit
AGCCGAGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GTTTTTTTCTATGGCTAATAAACTTTTTAATTCACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GAACTTACTAAAGGCACTTCCTTCCTGTTAAACCCCTGTTAACTCTCCATAAATTTGCTGATTCTCTGCTA20.14104372355430184No Hit
GCCTTAGATAGCAGCAGAAGGCTTTTTGGATTCTCCTCCTTGAAAAGATTCTCAGTTACCAAACGTCTCCA20.14104372355430184No Hit
GCAGGAGGCTAGCGAAGCGTACCTGGTGGGTCTGTTCGAAGATACCAACCTGTGTGCCATCCACGCTAAGA20.14104372355430184No Hit
ACGCGGGTGGGAAGCCAAGGTAGGCAGATCACTTGAGCACAGGAGTTTGAGACCCCCCCGAGCAACATGGC20.14104372355430184No Hit
TCCCTACACGACGCGGGCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GGAGGCAGAGGTTACAGTCAGCTGAAATCGCACCATTGTACTCCAGCCTGGGCAAGACAGTGAGACTCCGT20.14104372355430184No Hit
TTCCCTACACGACGCGGGGGCGTCGTAGTCTCCTGCAGCGTCTGGGGTTTCCGTTGCAGTCCTCGGAACCA20.14104372355430184No Hit
TTCCCTACACGACGCGGGGGCGTCGTAGTCTCCTGCAGCGTCTGGGGTTTCCGTTGCAGTCCTCGGAACCC20.14104372355430184No Hit
GGTCTTGGATGTCGGGTTCGACCACTTGGCCGATGGGAATGGTCTGTCACAATCTGCTCCTTTTTTTTTGT20.14104372355430184No Hit
GCTAATAAAGGCGCACATGACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GTAACCATATGGAAGCAACTTTTTTTGGTGTCTCAAAGGAGTAACTGCAGCTTGGTTTGAAATTTGTACTG20.14104372355430184No Hit
GAATAAATATCTTAAGCTAAGCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
TCCCTACACGACGCGGGGGTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
TTGTGCACATGTACCCTCAAACTTAAAGTATAATAAAAAAAAAAAAAAATAAAAAATAAAAAAAAAAAAAA20.14104372355430184No Hit
GGCCTTGAGTGCCCAGGAGTGCCCCACGTTCTGAGAAGGGGCCGGCCGGAGGGAGGGGACCCGGCAGGGAG20.14104372355430184No Hit
GCTCTGAGTAAAGAAGTCGCCGAAGCAGTGGGACACTAGACGCGGGGAAGGCTTCCTGGAGGAAGTGAGGC20.14104372355430184No Hit
TTCCCTACACGACGCGGGCAAATGGGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GAGGATGGAGATGAAGATGAGGAAGCTGAGTCAGCTACGGGCAAGCGGGCAGCTGAAGATGATGAGGATGA20.14104372355430184No Hit
TAATAAAGCAGATTATTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
GGAATGAGTGTATGCTGAATTTTTTCCAGGTAGGCAAGGATATTAGAATAGGTCTATCAGATTCAATGCAA20.14104372355430184No Hit
ACAAAATGCCACGTGTACCCCATAAATATGTAGAAATATTATATGTAAATAAATAGTTGGGCCTTCAAAAA20.14104372355430184No Hit
GGTCTGCAGCTCCCACTGCTCAGGCCACTGAATGGGTAGGAGCAACCACTGACTGGTCTTAAGCTGTTCTT20.14104372355430184No Hit
GAAGAAGGAGGAAAAAACCCAACATTTCTTGGACCAAAAGTATAGTGACTATCTGTTCATGAGAGAAATTT20.14104372355430184No Hit
GAAGAAAGGAGTCTTCTCCAATCATAGCAGCCAGCCTTCAGCCAGGGGCAGACTGCAGACTGACCAGATGT20.14104372355430184No Hit
GTTATGGGTGTCATTCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14104372355430184No Hit
ACATGAGTCCTTATGCATCCAGATGTTATAATAAAGTGGAATGGTCTCTTTAAAAAAAAAAAGGAAAGAAA20.14104372355430184No Hit
GGTTGCAGTGAGCTGAGATTGCACCATGCACTCCAACCCGGGCAACAGAGACCCTGTCTCAAAAAAAAGTT20.14104372355430184No Hit
GCTATTAGCCAGGATCATGGTGTAATAAGACATAACGTTTTTCCTTTAAAAAAATTTAAGTGCGTGTGTAG20.14104372355430184No Hit
GACCAAGGAGGAATTTCAGGGTGAATGGACTGCTCCCGCTCCTGAGTTCACTGCTACTCAGCCTGAGGTTG20.14104372355430184No Hit
GGAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGCCTTTGTAAAATTGACAGAGAGGGGAAACCC20.14104372355430184No Hit
GTCTTGATCTCCTGACCTTGTGATCCACCTGCCTCCACCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA20.14104372355430184No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCC20.14104372355430184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)