FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19716.06851549755302No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT141.1419249592169658No Hit
GTTTGTAGTGTTTTATCTTATCTACACATGGGGGACTGAAGAGTTCGAGAGATCCAAGAGGAAGAATCCAG100.8156606851549755No Hit
GGATCAACAAAATTGATAGACCGCTAGCAAGACTAATAAAGAAAAAAGAGAAGAATCAAATAGATGCAATA90.734094616639478No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG80.6525285481239804No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.5709624796084829No Hit
ACCCCGTCTCTACTAAAAACAAAGCAAAAATTAGTTGGGCGTGGTGGTGCATGCCTATAGTCCCAGATACC60.48939641109298526No Hit
ATATCATACCATAATGTGTCATAGCTATAGGCACAAGAAAAACAACAGTTTGTGAGAATATTATATAAGAT60.48939641109298526No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC60.48939641109298526No Hit
GGCTGCCAATCTCCCTGCTCTTGGTTCTCCTCTAGATTGAAGTTTGTTTTCTGATGCTGTTCTTACCAGAT60.48939641109298526No Hit
GCTCATGCCTGTAATCCCAGGACTTTGGGAGGCCAAGGCGGGCGGATCACAAGGTCAGGAGATCGAGACCA60.48939641109298526No Hit
AGTAGAAGGAAAGAGCTTCAGGAAGTGAGGAGTTTATTAAAATTCCTTTGAAGCATTTCAATTCAGTAAGA60.48939641109298526No Hit
CTATCAAGATATTAGTAAGAAAGGATCATGTTTTGAAGCAGCAGGTCCAGGTCACTTTGTATATAGAATTT50.40783034257748774No Hit
ATTTCAAGCAGACAAAAATAGAATAGAATAATAAACCCCATTGTTCTCATTATCCACCTTCAGCAATTAAC50.40783034257748774No Hit
GTCAGGCATGGGAGTCATTGTCCACATCATCGAGAAGGACAAAATCACCACCAGGACACTGAAGGCCCGAA50.40783034257748774No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.40783034257748774No Hit
TTAGTGACGTTTACATTTTGAAATAAAATTTATGATTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.40783034257748774No Hit
GCCTGATTGATAACCTGATCCGGCGGAGGCCGATCGCGACGGCTGGTTTGCATCGCCAGCTTCTATATATT50.40783034257748774No Hit
GTTTAATCATGGAAAACTCAAAAAGAAGGGGGGAAATGGTCAGTTTAAGCCAAAAGAAACTTTCTAAACAA50.40783034257748774No Hit
CCCCATCTCTACTAAAAATACAAAATTAGCCAGGCGTGGTGGTGCATGCCTGTAATCCCAGCTACTCAGGA40.3262642740619902No Hit
AGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCG40.3262642740619902No Hit
TTGTGGAGGCCGTTGGTTCCGCCCACTCATTCCTTCTCGTGCACTGCTTTCTCCTTCACAGCTAAGATGCC40.3262642740619902No Hit
GCATGTGCCTGTGGTCCCAGCTACGAGGGGCTGAGGTGGGAGGATTGCTTGAGCCTGGGAGGTCGAGGCTG40.3262642740619902No Hit
CTTTTCATGACTGCGAGGACGCCGCACGCGAGCTGCAGAGCCAGATACAGGAGGCCCGAGCCGACTTAGCC40.3262642740619902No Hit
CTTCTTGGTGTCATTGCTCAAGAAAAACTGGCAGAATGTCCGGGCCTTATATATCAAGAGCACCATGGGCA40.3262642740619902No Hit
GTTTTTAATCATCAGGACTATCTCACCCTCCCACTCCTGTTTTTAAAACTCAGAATTCTTTCCTAAGAGCC40.3262642740619902No Hit
ACAAAAGATAAAAACATTAGCTGGATGTGGTGGCACGTGCCTGAAGTCTCAGCTCTCTGAGAGGCTGAGGC40.3262642740619902No Hit
GGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGAGGAAGAGGAGGAGGAAGAAGAAGGTG40.3262642740619902No Hit
GTTTTTTTAATGTGTTTGCAATAGTCTTATTAAAATAAATGTTTTTTAAATCTGAAAAAAAAAAAAAAAAA30.24469820554649263No Hit
GTTCCGAATTCAATAGAAATATTCTGTACAATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.24469820554649263No Hit
GTTCTGATTAATAAAGGATCGTGAAAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.24469820554649263No Hit
GCCAGTGCAGCTTGGATCTCGGGGGTGTGGGGCCCTGTGCCTTCCTGAAGTGCTGGCAGCCCAGTGGCACC30.24469820554649263No Hit
GCCGCTTCGCAACACGTCTTCCGTTTCTACCGGGAATCGCTGCAGAGGCGTTACTCCAAGAGCTGAGGCAA30.24469820554649263No Hit
GATATGTGGAGTGAAACTTCGATATTTGAGATGTTTACCGTTTCTAAGATAATATCCCTTTATTTTTGTTT30.24469820554649263No Hit
CTTCCAAGCTGGCTTTTCCCATTTCAATTCCTGTGATTTATGCCAATAAAGTTTGCCCATGATTTTCAAAA30.24469820554649263No Hit
GCCCTTGCCCTTTCCCTTTCCCTGGGGTCCAAACCACATGTGTCCTGCCTCTCCTGGCCCTACCACATTCT30.24469820554649263No Hit
ACATCTCTTGAAGCCAAACTCCACCTTCTGTGCTTTTTGCTTGGGATAATGGAGTTTTTCTTTAGAAACAG30.24469820554649263No Hit
GACTAAGAGTAACTGTGTGATCTGTTAAGGGGTGGATAACATAATATGCAGCTTAGGATGCTATTTTGAGA30.24469820554649263No Hit
GCCCTGGTGTAGTTTCTTCATTTCAGGAAGACTGACAGTTGTTTTGCTTCTTCCTTAAAGCATTTGCAACA30.24469820554649263No Hit
GAACAAGGGGTCCAGCTTGCGGGGGACCCTCCCCAGCCCATTCCTGCTGTCAAACAAACAAAACCTTGCAA30.24469820554649263No Hit
GCTACTCGGAGGCTGAGGCAGGATAATCGCTTGAAACCGGGAGACAGAGGTTGTGGTGAGCCGAGATCACG30.24469820554649263No Hit
ACACGACGCGGGAGTCAGGGAAGGTAACCCTGAAGCAGGACTTGTAAAGCAAATAAGATTGGGAGGCCAAG30.24469820554649263No Hit
GCTGCTCTGAGGTAAAGGTCCATGAACCCTGCCCCACTCCTGTCAGCCCCTCATCAGCCTTTTCAGCAGGT30.24469820554649263No Hit
GGCAGTGGAGGTTGCAGTGAGCCAAGAACGCGCCATTGCATTCCAGCCTGGGCAACAGGAGTGAAACTCCA30.24469820554649263No Hit
CCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAGGCACTGTTCATCTCA30.24469820554649263No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA20.1631321370309951No Hit
GACTAATACAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
TGTCACAAGCAAATGTGTCGAGAGCAAAGGCAGTCCGAGCCCTGAAGAACAACAGTAATGATATTGTAAAT20.1631321370309951No Hit
CTTTTCATGACTGCGAGGACGCCGCACGCGAGCTGCAGCGCCAGATACAGGAGGCCCGAGCCGACTTAGCC20.1631321370309951No Hit
TCCCTACACGACGCGGGAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
GCATCTGTCCATTTTCCTTGAAGGATGAGCCTTTAGAAACCTCTGACAATAAAGTTTATTTTACACCCAAA20.1631321370309951No Hit
CCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTA20.1631321370309951No Hit
TATCAAAGCAGTTGTCATTTGGAAGTCACTTGTGAATAGATGTGCAAGGGGAGCACATATTGGATGTATAT20.1631321370309951No Hit
GTTTGTAGTGTTTTATCTTATCTACACATGGGGGACTGAAGAGTTCGAGAGCTCCAAGAGGAAGAATCCAG20.1631321370309951No Hit
GTTTTGCAGCAATTGTTGCATATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
AGATGGTCCTCCTGCCTCAGCTTCCAAAGGCACAGGCCAAGTTGTAGCTTTGTCCCTTGCCATCATGCCCA20.1631321370309951No Hit
CTAGTAATGGGCTTTATGTGCTAGATTTTAATATCCTTGAGCCTGGGCAAGTGCACAAGTCTTTTTAAAAG20.1631321370309951No Hit
ACTTGAGCTTGGGAGGTGGAGTTTGCAGTGAGTGAGTGGAGATCGCGCCACTGCACTCCAGCCTAGGCGAC20.1631321370309951No Hit
TGCTTCCCCAGGCTATAAATTTGTTCTCACAAAGCAACATCAATAAATCAAAACTGTCTCTCCCAAAAAAA20.1631321370309951No Hit
GGATTTGTAACTCCAGAAACATTCTATAGTGTGCCAGAGCAAAAGGCAAATACACAAAATATTATTTAAAT20.1631321370309951No Hit
GCATAGAAGAGACTTTTTATTTATTCTAGAATTGCAGAAATGACTACACTGTGCTATACCAGAGAATTCCA20.1631321370309951No Hit
GCTGCCTGCCTTGGGGAGGAACCATATCTCCCTCTGGGCCGCTTCTGGCCTCTTGGAGCCATGGGCCAAAG20.1631321370309951No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC20.1631321370309951No Hit
TCCCTACACGACGCGGGAGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTA20.1631321370309951No Hit
GGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA20.1631321370309951No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
GCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
CGCGGGAGGGTAGAGCAGGTGATTGGAATGAGTCAGGGTGGAGCAGGTGATGAGAATGAGTTAGGGTGGAG20.1631321370309951No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
GGCTGCAGCTAAGGAGTTAGAAGAAGTAATGAAGCGAGTACGAGAAGCTCAGTCATTTATCTCAGCAGCTA20.1631321370309951No Hit
CTTTTTGTATTTTGCATCTGACCCCGGGGGGCTGGGACAGATTGGCAATGGGCCGTCCCCTCTCCCCTTGG20.1631321370309951No Hit
AACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGT20.1631321370309951No Hit
GGATTATACTTAAACTTCTAAAGAATGACCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
GGTGCAATCTCAGCTCACTGTAACCTCCGCCTCCCAGGTTGAAGTGATTCTTGTGCCTCAGCCTCCCTAGG20.1631321370309951No Hit
GAAACAGAAATGACCACGTGAAATTTGCCTCTGTCCAAACATTTCATCCGTGTGTATGTGTATGTGTGTGA20.1631321370309951No Hit
TCCTATGGCCATGACCCAGAAGTATGAGGAGCATGTGCGGGAGCAGCAGGCTCAAGTAGAGAAGGAGGACT20.1631321370309951No Hit
AGTGAGCTCTAGGCTGTAGAAATTTAAAAACTACAATGTGATTAACTCGAGCCTTTAGTTTTCATCCATGT20.1631321370309951No Hit
GGGAAAGAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1631321370309951No Hit
GCCCTGGGGGTCATCTGGGGCCAGGGCTCTCTGGGTGCCTTCCTGCTGCCCCAGCCAGGGTTGGAGTCTTA20.1631321370309951No Hit
AGCTTCACCCCACGTGGCTTGGTTCCCAGGGACAGTCAGGGCCTCGGGGGCCCAGCTATGTACAACGAAGC20.1631321370309951No Hit
CCCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAGGCACTGTTCATCTC20.1631321370309951No Hit
GTCCTCCTTCCTCATTACAGTGAAACCAACAATATGCAGAGGTTGGGGAGAGATGCCTGTTCTTCCCCCAG20.1631321370309951No Hit
ACAATGAAGCCCTCAAGGGCTGAAAATAAATAGGGAAGATGGAGACACCCTCTGGGGGTCCTCTCTGAGTC20.1631321370309951No Hit
CTAAACCATTGTGCATTCTAGAGTGCATATATTTATATTTTGCCTGTTAAAAAGAAAGTGAGCAGTGTTAG20.1631321370309951No Hit
CCTGTAGTCCCAGCTACCTGGGAGGTTGAGGTGGGAGAATCGCTTGAACCCGGGAGGCGGAGGCTGCAGCA20.1631321370309951No Hit
CCTTTCTGCACTCAAGCACAGCGAGAAGTACGTTATGCGTGAAAACAAAACAACTTTGCATTAAAATCCAT20.1631321370309951No Hit
CTACGAGCAGCTGATGCAGCTGTACAGTGCGCGCCAGCGGCGGCGGCTGAACCGGGGCCTGCGGCGGAAGC20.1631321370309951No Hit
GGAATGAGCCGGACAGCCTGGCTGTCATTGCTTTCTTCCTCCCCATTTGGACCCTTCTCTGCCCTTACATT20.1631321370309951No Hit
ATTCAGCTCTGTCAAGAATGGTCCAGGTTCTTCTAGATGATCTGCACAAATGGTTCCTCTCCTCCTTCCTA20.1631321370309951No Hit
GGGTTTAATTCGGGTGGGGAATATAACATATCAAAATACATGAACAAATGGAAAGTTACTTCTAGAAAAGC20.1631321370309951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)