FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90232.63386396526773No Hit
GCATTTATGAATGGGGTGAATGGGGCTATTGTCGACGGCACAGCTAATGCCTGAACCCAGCCCCTGTCGGC361.3024602026049203No Hit
GGCCTTTATAATTTTACTCACTCACTCTGATTTATGTTTTGATCAAATTTGAACTTCATTTTGGGGGGTAT301.085383502170767No Hit
ACTTAATGCAGTGGGCAGTGTCCACGTTTTTGGAAAATGTATTTTGCCTCTGGGTAGGAAAAGATGTATGT301.085383502170767No Hit
GTTCAAGACCAGCCTGGGCAACACAGTAAGACCCACGTCTCTACAAAAAATTTAAAAAATTAGCCAGGCGC240.8683068017366137No Hit
TACTTTTACTGTCTATATATATAGGGAGCACTTTAAACTTAATGCAGTGGGCAGTGTCCACGTTTTTGGAA240.8683068017366137No Hit
CCCAATGCTACTCAGGAAGAATTGAAAAAGGCTTATAGGAAACTGGCTTTGAAGTACCATCCTGATAAGAA220.7959479015918958No Hit
GAAAATGGTGGAAAAGGACCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACAGAA220.7959479015918958No Hit
TACTCTGCTGTGGTCATGCCCACTCTTTGGGAGTATATTCCCTTTATATATATTGAGTATTGTACCACTTG210.759768451519537No Hit
CTTTTACTGTCTATATATATAGGGAGCACTTTAAACTTAATGCAGTGGGCAGTGTCCACGTTTTTGGAAAA210.759768451519537No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA200.723589001447178No Hit
GCTGCAGACCCTCGAACATGTGGACCTGGACCCTCAGGAGCCCCCGAGATGACTGCAGGGGGCTCAAATGC200.723589001447178No Hit
GATAAATGGAAGACTCTTGAACTTGTGAACTGATGTGAAATGCAGAATCTCTTTTGAGTCTTTGCTGTTTG200.723589001447178No Hit
GCTTTTAGTTTCTCTTAATCAAAATTACTAGATGATAGAATTCAAGAACTTGTTACATGTATTACTTGGTG200.723589001447178No Hit
ACTTACTACGATGTTTTGGGGGTCAAACCCAATGCTACTCAGGAAGAATTGAAAAAGGCTTATAGGAAACT200.723589001447178No Hit
GGCCCTTCTGGTGGGCATTTGGTATGTCCTTTCTCTTGGGGTGATTTCTGATGTTTTTACTCTATATAGTG200.723589001447178No Hit
ATAACAAGAAAATTGAAAGATGGGAAATTAGTGGTGGAGTGTGTCATGAACAATGTCACCTGTACTCGGAT190.6874095513748191No Hit
GTCTATATATATAGGGAGCACTTTAAACTTAATGCAGTGGGCAGTGTCCACGTTTTTGGAAAATGTATTTT180.6512301013024602No Hit
GTCTCCCTCTGTCCCCAGGCTGGAGTGCAATGGTGCGATCTTGGCTCACTGCAACCTCCAGCTCCCGGGTT180.6512301013024602No Hit
GGTGGGGACAGACTTGCTGGAGGAGGAGATCACCAAGTTTGAGGAGCACGTGCAGAGTGTCGATATCGCAG170.6150506512301013No Hit
GCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACCAAAAAGGATTATGAAGAAATTGGACCTAGCA170.6150506512301013No Hit
ACGTATATGCACCTAATAACATACCATCAAAATATATAATGAAAAAATTGACAGGTCTCCAAGGACAAAAA170.6150506512301013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.5788712011577424No Hit
GTTTATTCCTAGGTATCTTATTTTTATTATTACTATTGTAAATGGAATTGCTCTTTTGATTCTTTTTCAGA160.5788712011577424No Hit
GCCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTATGAGCCACAACGCCCAGCCATCATTTATCTTTT160.5788712011577424No Hit
GAGTCCAGGACCAGATGGATTCACAGCCGAATTCTACCAGAGGTACAAGGAGGAACTGGTACCATTCCTTC150.5426917510853835No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG140.5065123010130246No Hit
GTTCATGTTCATATGTACTTGTTTGCTATGACTACATTTTGAGGTTTTGTAAAACTGTTATTTTTTTTTTT130.47033285094066574No Hit
GTTTTTGGGGAAGCCAAGATCGAAGATTTATCCCAGCAAGCACAACTAGCAGCTGCTGAGAAATTCAAAGT120.43415340086830684No Hit
CTTCAAAGGACATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTA120.43415340086830684No Hit
GGGTAGGAAAAGATGTATGTTGCTATCCTGCAGGAAATATAAACTTAAAATAAAATTATATACCCCAAAAA120.43415340086830684No Hit
ATTAACATCAGGCACGATAAAAGCCATGTTGAGTGGCCCAGGTCAGTTTGCTGAGAACGAAACCAATGAGG110.3979739507959479No Hit
GTGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGACGGATCACTTGAGGTCAGGAG110.3979739507959479No Hit
CTTATACACAATTCATTCTTTGCAGCTAATTAAGCCGAAGAAGCCTGGGAATCAAGTTTGAAACAAAGATT100.361794500723589No Hit
GTTGTCTCTGATGCAGCTTATACGAAATAATTGTTGTTCTGTTAACTGAATACCACTCTGTAATTGCAAAA100.361794500723589No Hit
GCTCTGCTGCCATGAAGGTGAAGATTAAGTGCTGGAACGGCGTGGCCACTTGGCTCTGGGTGGCCAACGAT100.361794500723589No Hit
TCACTGATCTTTCTGGCAAGGAAACCATCTGCCGTGTGACTGGTGGGATGAAGGTAAAGGCAGACCGAGAT90.3256150506512301No Hit
CTTCTGGGTTGGGCCTTCTCATAGTTATCCGTTGTTAAAAGTTCTATATTTCTACAAACCTTCTTGTGCGA90.3256150506512301No Hit
AGCCCCCGCGCCCGGCAAAAAAACAGCATGTTAAGATGCTGGTGAGGCTGCTGAGAAAAGGAAATGTTTAT90.3256150506512301No Hit
GTGGCTGACCTACTGGGTAGTGTATGGTGTGTTCAGCATTGCTGAATTCTTCTCTGATATCTTCCTGTCAT90.3256150506512301No Hit
GCTCATTGTATAAGCCCCGAGGCCTAGAGTGGCAGCCAAGAGCCATCTGAAGCCACCTCTCTGCCTGTTCC90.3256150506512301No Hit
TTTCCCCCATCCTGGTTTAAGTATAAAGCACTGTGAATGAAGGTAGTTGTCAGGTTAGCTGCAGGGGTGTG80.2894356005788712No Hit
CGTTCCTTGTTGCCACTTTGGGTATTCTGAGATTTTCTCTTGCATGCCCTTAGCTTTACAGCTTTTGCATT80.2894356005788712No Hit
GGGCTGAACTGCTCTACAAGCGCATCATCCGTCCTTTCTTCCTGAAGCACGAGTCCCAGATGGACAGTGTG80.2894356005788712No Hit
ATTCAATAATTCTCTACTTGAACAAGAATACTTAAAGATAACTTTTGTACATTTGCAACCTGAACTATCTT80.2894356005788712No Hit
GCCTGCACCGGGCAAACCCCGTACCTTCCCAGCATCGGCTCAGCAACCCACGTGCATCCAGGCCGGTCAAT70.2532561505065123No Hit
ATTTTAACTTTATTTGTGAATAGTGATTGTGGCATGTTTGGGGTGTTATTTTAATATTTCACGATACTAAA70.2532561505065123No Hit
CGCTGAGGCCGAGCCTGCACTGGGGCCACCCAGCCAGGCCTGGGGGCAGCCTCTCCCCAGCCTCCCCGTGC70.2532561505065123No Hit
GGCTTTGATGCCACAAACATTCTCAACAAGCTGCGGGCTCGGCATGCCCAGGGGGGTACATGGTATGGAGT70.2532561505065123No Hit
GCCTAATACATTAAAGCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2532561505065123No Hit
GCCCAGGAGTTTGAGGCTGCAGTGAGCTGATATGCCACTGCATTCCAGCTTGGACAAAAGAGTAAGACCCT70.2532561505065123No Hit
CAACACACAGATCTCCTACTCCATCCAGTCCTGAGGAGCCTTAGGATGCAGCATGCCTTCAGGAGACACTG60.21707670043415342No Hit
ATGTTCAGGATTGTTTTGATACCTGTATTTATAATAAAAAATGTTGGGGGGAGTTGATGAATTCCTGTTAA60.21707670043415342No Hit
CATACATACTGTATGAAGCTTGTCCTCTGACTAGGTTTCTAATTTCTATGTGGAATTTCCTATCTTGCAGC60.21707670043415342No Hit
GGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCC60.21707670043415342No Hit
GCCCGCCTGCTCCCAGGTGAAATAAACAGCCATGTTGCTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.21707670043415342No Hit
GTATGTATGATGACTTCAGTGTGCAAGATGAAGTTTAATACCTGTAAAAACTACAAAGAAGTTCCCCTAGC60.21707670043415342No Hit
GATGAGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.21707670043415342No Hit
AAACGGTTTAGAGACTAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.21707670043415342No Hit
CTTTGACTTCTTGCATGCGAGGGACCATTGCGTGGCCCACAAACTCTTTAACAACTTGAAATAAATGTGTG50.1808972503617945No Hit
GGGCATGGTGGCACATGCCTACAGTCCCAGCTACTTGGGAGGCTGAGGCAGGTGAATGGCTTGAATCCAGG50.1808972503617945No Hit
GTCTACAAAAATGAAAAACCAAAAAGCAAACCAAAAGAAAAATCTGAAATTTCCATCTGGGGATTAACTTC50.1808972503617945No Hit
TCCCTACACGACGCGGGGGAGAGCTTTGGATAAGGTATTGAATCTCTCTGAGCCTCAGTTTTTCATTTGTT50.1808972503617945No Hit
TCCCTACACGACGCGGGTAGGTAGTGAGACAAAATTGATGATCCATTAAGTAAACAATAAAAGTGTCCATT50.1808972503617945No Hit
GGTGTTCAAAGGGGCAGTGAAGATTCGGAAAGGGCAAGCAGTGACCCTGATGATGGATGCCACCAATATGC50.1808972503617945No Hit
GTCCATCCCCTTCCCCCATCCCTACCACTGTGGCCCCAAGAGGGGCGGGCTCAGAGCTTTGTCACTTGCCA50.1808972503617945No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1808972503617945No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATT50.1808972503617945No Hit
GTGGAAGTCACCATTGCAGATGCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTGTTAAAAAAAAAA50.1808972503617945No Hit
TTTTAGTTCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1808972503617945No Hit
GGTTTCCCGTGTTGGCCAACCTGGTCTTGAACTCCAGACCTCATGTGATCCTCCCGCCTCTGCCTCCCACC50.1808972503617945No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA40.1447178002894356No Hit
CTTTATTTCTATTATGAATATTTTAGCGATTATAATCAGCTATTCCACTGAGTATTAGATTTGGTCACTAA40.1447178002894356No Hit
CCCAATGCTACTCAGGAAGAATTGAAAAAGGCTTATAGGAAACTGGCTTTGAAGTACCATCCTGATAAGAC40.1447178002894356No Hit
GTCTATTGGTCTCTTTGCCAGCCCTCCCCTTTCCCAATGAAAGCCATGTCAAATTAAAAAAAAAAAAAAAA40.1447178002894356No Hit
GGACCGTACAGACCTGCCTAACATGCCAGCGCAGCCAACGCTTCCTCAATGATCCCGGGCATTTACTCTGG40.1447178002894356No Hit
AGAATTAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1447178002894356No Hit
GATATAAATTTACCCAAAGATGATGACTTTGATTGGATAATTAGTAAGGTCTTTTTGTTATTTTTCATCGT40.1447178002894356No Hit
CTCTCTGGTAGCATTTTGTCCTCACACACCCATCTACTATGTCCAACCGGTCTGTCTGCTTCCCTCACCCC40.1447178002894356No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC40.1447178002894356No Hit
GCCCTACTCCCGCCGCTCTGAAAATAATAAACGACTTTATTCTTGGAAAAAAAAAAAAAAAAAAAAAAAAA40.1447178002894356No Hit
CGTATTTTCTTTATTCACATCCCAGGAGCATCCATTTTAAGGAACTATTCTTTGGAAAAAAAAAAAAAAAA40.1447178002894356No Hit
ATCTACAATCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
TTCCCTACACGACGCGGGTTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
CGTATTTTCTTTATTCACATCCCAGGAGCATCCATTTTAAGGAACTATTCTTTGGAAAAAAACAAAAAAAA30.10853835021707671No Hit
GAATAAAGTAATCTTATATACAAGCTTTGATTAAAACTTGAAACAAAGAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
GATGCTTGGTTTAATGCTTGCAATCTGAGCTCTTGAACAAATAAAATTAACTATTGTAGTGTGAAAAAAAA30.10853835021707671No Hit
GCACTCCAGCCTGGGCGACAGAGCGAGACGACTCCGTCTCAAAAAAAAAAAACAAAACAAAAAAGAAAAAA30.10853835021707671No Hit
GTATAATATTTCATTGTGTGAATAAACCACATACTGTTTATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
AAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTCGGGTATGAAGA30.10853835021707671No Hit
CCCCTCTCTGGAGGCCTTTCTAGGGGATGTTTTTTTATAAGCCAGATGTTTTTAAAAGCATATGTGTGTTT30.10853835021707671No Hit
CACCATCGATCATGATGTAGAGTGTGGTTATGAACTTTATAGTTGTTTTATATGTTGCTATAATAAAGAAG30.10853835021707671No Hit
CCAAATAAACTCAGGAACGCCCCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
GCCTCCTGGGTAGCTGGGATTGCAGGCGCACGCCACCACGCCCGGCTAATTTTTGTGCTTTTAGTGGAGAC30.10853835021707671No Hit
ATCCCCTCAATGCCTCATAATTCTGGAATGCCTGTTGTGAAACATGTCAGTGCACAGTGTCTCCTAAAAAA30.10853835021707671No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
GTAAAGAAAGGATTAAAAATTTAAGATGTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
ACACGACGCGGGAAAGCTGATCTGTAAACCCTTACCCCTTGCTGTTCAGAGAGCTACTCTTTGTAGTGTTC30.10853835021707671No Hit
CCATTGAAGAAGGGAGTTTGAAAGTTGTTGTATACTGTTAACGATTGTCTGCCCATGTCCTGCCTGAAATA30.10853835021707671No Hit
GGAAAATAAATATGAAGCAAGTCAAACTAGATGCATACACTTGTGTAGAAATCAATAATCAATTAATAGAA30.10853835021707671No Hit
CAATAAACCTTGAACAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
CTGTACTCGTTCATCAAGGGTGACACATCTGGAGACTACAGGAAAGTACTGCTTGTTCTCTGTGGAGGAGA30.10853835021707671No Hit
TTACAAAGCATCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
TCCTTGGATGCTGCATTCATAGGAGAATTGAATAATTTCTATCAATATGTATTTATCATTAAATTTTTTTT30.10853835021707671No Hit
GCCTCAGTGCCCGGCCCAAGCTCAAGGCCTTCCTGGCCTCCCCTGAGTACGTGAACCTCCCCATCAATGGC30.10853835021707671No Hit
CTGATGTTCTGCTTGGTTATTTACTTTTAATTCATGTTGGAGCTCCTCCTGCCACTGCAAGAGCAGGAGCT30.10853835021707671No Hit
GTGGCAGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10853835021707671No Hit
GCTACTCGGGAGGCTGAGGCAGGAGAATGGCATGAACCGGGGGGGCGGAGCTTGCAGTGAGCCGAGATTGA30.10853835021707671No Hit

[OK]Adapter Content

Adapter graph