FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32424.269662921348313No Hit
AGCCTGGAGTCAGGACAAATGGATCGGGCTGCAGAGGGTTAGAAGCGAGGGCACCAGCAGTTGTGGGTGGG302.247191011235955No Hit
CCTCTGGTCTATCTCATGAAACCTCTTCTCAGACCAGTTTTCTAAACATATATTGAGGAAAAATAAAGCGA241.7977528089887642No Hit
GGTAAATACAAGTGAACAAAATAAGCAACTAAATGAGACCTAATAATTGGCCTTCGATTTTAAATATTTGT231.7228464419475655No Hit
GGGAAAGCGCCGAGATGACGGGCTTTCTGCTGCCGCCCGCAAGCAGAGGGACTCGGAGATCATGCAGCAGA221.647940074906367No Hit
CTTCAGAGGAGCATCTCTGAGGGTTCTAGCTCTTTTAACACCCCTTTTCCCCCTGCTGGCCCAGAGGGAGA211.5730337078651686No Hit
ACATTTATGACCTAAGTCAGGTAATATACCTGGTTTACTTCTTTAGCATTTTTATGCAGACAGTCTGTTAT191.4232209737827715No Hit
TCCCAGCAATCGCTTGGTTCAGTTTCATTCAGCTCTCTATGGACCAGTAATCTGATAAATAACCGAGCTCT171.2734082397003745No Hit
GTGAGTACCTGGACCATCACTTAACTGATGCTCCGGGGTAGGACTGCAGGTTTCACATGAACCTGTTCTAG171.2734082397003745No Hit
GCTCCTTTAAGATTGTCCTGTGACAGCAGCCCCAGCGTGTGTCCTGGCACCCTGTCCAAGAACCTTTCTAC161.198501872659176No Hit
ATCTCTGGGGACTGAGGAGCAGCAGGAGGGGGGAGGGAAGGGAGCCAAGGGATGGACATCTTGTCATTTTT161.198501872659176No Hit
GCACCTGGGTGGGTTTGAGCTGGACAGAAGCTTAGAGACAAAGGCTTCAAGAAGCAGTGGCTGCAGGGAGT151.1235955056179776No Hit
GAAAAAAATATGCTTTCAACTTTGTGAGTGAATTTCGTGTTGTGTAAGTTATATGTCAAATCTTTGAATTT141.0486891385767791No Hit
GTGGTAATTAGCTTGTAACCAGGGTATGATCTGCTATTGTTATTTCTCCTCTTTATTGGAAAAAGGCCTCA120.8988764044943821No Hit
AGCTGGTGGAGCTCAGCACAGCCCTGGAGCACCAGGAGTACATTACTTTTCTTGAAGACCTCAAGAGTTTT120.8988764044943821No Hit
GTTCTAATCACACTAATCAAAAAGAAACGAGTATTTGTATTTATTAAACTCATTAGTTTGGGCAGTATAAA120.8988764044943821No Hit
TTTCAAAGGTTTCCCAAACCACTCCTTATGATCCAGTGAATATTCAAGAGAGCTACATTTGAAGCCTGTAC120.8988764044943821No Hit
ACTGTATACCGATCAGGAATCTTGCTCCAATAAAGGAACATAAAGATTTAAAAAAAAAAAAAAAAAAAAAA110.8239700374531835No Hit
CTTCAGAGGAGCATCTCTGAGGGTTCTAGCTCTTTTAACACCCCTTTTCCCCCTGCTGGCCCAGAGGGAGC110.8239700374531835No Hit
GCCCTGGACTCTCCTCGGCGCGGTTAACCTCAGCCCGCCCTGCAGGGCTGTTGAAGGCCGTGGGCCGGACG110.8239700374531835No Hit
GTTTACCCATGAAGATTGGTGGGATTTTTTGTTTGTTTGTTTTGTTTTGTTTGTTGTTTGTTGTTTGTTTT110.8239700374531835No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG100.7490636704119851No Hit
TCCCTACACGACGCGGGGAATTAAGCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.6741573033707865No Hit
ACAGCATGTATTTTCTTCTCTTCTGAAAGTTCTGGCTTGTAGACCCCTCCCCTCCTTTGCAAAGGTATGGG90.6741573033707865No Hit
GTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAACGATTGAACAGGAGA80.599250936329588No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.599250936329588No Hit
CCTTTATATTTGGGGTCCTTTTTTATACAGGAAAAACAAAACAAAGAAATAATGGTCTTTTAAAAAAAAAA70.5243445692883896No Hit
GTCTGGGGTAGGGCACAGGGACCTGAAAATTTAGAAATAGGCAATTAGCTGGGCGTAGTGGCTCATGCATA70.5243445692883896No Hit
CTTGTCAAGGAATGTTATCAGTATTAAGCTAATGGTCCTGTTATGTCATTGTGTTTGTGTGCTGTTGTTTA70.5243445692883896No Hit
GTTTGTATGTTGCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.5243445692883896No Hit
CTTATAAAATGTTCAGACCTCTCTCTACATTCTCTTTACAATGTAATGTCTCTAAGTGACTGGTTTCCCAA60.44943820224719105No Hit
GCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGAGAGGCGGAGGTTGCAGTGAGCCAAAATTGC60.44943820224719105No Hit
GACGTGCTCCTGCTGACCTTCTGCGGCTCCGGGCTGTGTCCTAAATGCAAAGCACACCTCGGCCGAGGCCT60.44943820224719105No Hit
GTTTCCCTCCTTTTTTGTTTGTTGATTAGCAAATTTTTGATTCTCCATTTTCCAAAAGTAAGAGACTCCAG50.37453183520599254No Hit
GCCCTGGACTCTCCTCGGCGCGGTTAACCTCAGCCCGCCCTGCAGGGCTGTTGAAGGCCGTGGGCCGGCCG50.37453183520599254No Hit
GAATGTTTATTCTTCAGTCCAAGGAAGCAATTCACAGTCAGCTGTTAGAGAAGCAAAAAAAAAAAAAAAAA50.37453183520599254No Hit
CAATAAAGCCTGCTACCTGGCAGTACCCCTGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.37453183520599254No Hit
CTATTTATCTTTAATAAAGCACTGGATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.37453183520599254No Hit
ATAATAAATGGGTTAATTTATGTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.37453183520599254No Hit
GTTTGTCTCAAGGAAATAGAGATTGTGATATGTTCTAGTACAATGAAGTGTAATCATGTAAAATAAAAGCT50.37453183520599254No Hit
GGAGGCAGAGATTGCAGGGAGTCGAAATCAAGCCACTGCACTCCAGCCTGGGCGATGGAGTGAGAGTCCAT40.299625468164794No Hit
CCCCTTCACTGCAGAGCCTTGCCTTTCCCCTCTGCCTGTTTCCTTTTCCTCTCCCAACCCTCTGGTTGGTG40.299625468164794No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.299625468164794No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT40.299625468164794No Hit
CTATGAGACAATTGACAAGGAACTTATTGATCATTCTCCGAAAGCAACTCCTGACAATTAAGCATTTTTTT40.299625468164794No Hit
CCCTCTCTCCAGAGCAGCAGGAAATGTTGCAAGCATTCTCTACCCAGTCTGGCATGAACCTCGAGTGGTCC40.299625468164794No Hit
ATACAGATCAACCCACAGAACAAAGCGGATTTCCAAGGCATCTCCCCAGAGCGAGCCTTTGCTGATTTTCT30.22471910112359553No Hit
ACGCGGGCAGCCAACTAAGATCGGAAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22471910112359553No Hit
GAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAGACGCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA30.22471910112359553No Hit
ACAGCATGTATTTTCTTCTCTTCTGAAAGTTCTGGCTTGTAGACCCCTCCCCTCCGTTGCAAAGGTATGGG30.22471910112359553No Hit
GCCTATATTAAGGCACATTTGAATAAATTCTATTACCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22471910112359553No Hit
GGACTGTCCCCTGTCCCCGCATCTCCCCTGGGACCAGCTGGAGGGCCACATGCACACACAGCCTAGCTGCC30.22471910112359553No Hit
GAATTAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22471910112359553No Hit
AAGTATCTCAGCTCAACTGAAGAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22471910112359553No Hit
ACGTAGGATCAGACGAGGCCATGCTCGTTTGGCATTATCTCAAAACCAGCAGTCTTCTGGGGCCGCTGGCC30.22471910112359553No Hit
ATTAAAAATACTTACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22471910112359553No Hit
GACGTGCTCCTGCTGACCTTCTGCGGCTCCGGGCTGTGTCCTAAATGCAAAGCACACCTCGGCCGAGGCCG30.22471910112359553No Hit
GGGAAAGCGCCGAGATGACGGGCTTTCTGCTGCCGCCCGCAAGCAGAGGGACTCGGAGATCATGCAGCAGC30.22471910112359553No Hit
GTTCTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22471910112359553No Hit
GTATTTATTTAGCTTAAGGTTTGGCTATCATGGTGAGAACTCCTATGTACCCTGCTCAGGTCCTGGTAAAC20.149812734082397No Hit
CCTTCATCCCTCTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCT20.149812734082397No Hit
CCCATGGTATTTAACATAAAAAAAATAAAACTGTTAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
GACGTGCTCCTGCTGACCTTCTGCGGCTCCGGGCTGTGTCCTAAATGCAAAGCACACCTCGGCCGCGGCCT20.149812734082397No Hit
CTTCCTGTTAAAACTTCATTTTTTCAAACTTTTTGAGTTATGCAATATATAAATAAACAGTAAGAATTTTA20.149812734082397No Hit
GGAGAATGGCGTGAACCCGGAAGGCGGAGCTTGCAGTGAGCCGAGATTGTGCCACTGCACTCCAGCCTGGG20.149812734082397No Hit
CCTCTGGTCTATCTCATGAAACCTCTTCTCAGACCAGTTTTCTAAACATATATTGATGAAAAATAAAGCGA20.149812734082397No Hit
GTGAAATGGATCTTCAGACACTTCATGTTACTGTCCCTTTTCCCTCCAGGACTGCAGGAGGTGCTGTGGGT20.149812734082397No Hit
GACCAGGAGAGAAGCAAGGTCAAGAAATCCCACAGTTTGATGTATTAAAGAAATGACTTATTTCTACTCAA20.149812734082397No Hit
CTTTCAGAGAGCCCCAATTCTGCTTTCCCACAAAATAAACCTAATGCCATCAGGCAAAAAAAAAAAAAAAA20.149812734082397No Hit
AAGTAGAGCCCGGGACCGCCAGGCCACCACCGGCCGCCTCAGCCATGGACGCGTCCCTGGAGAAGATAGCA20.149812734082397No Hit
GCTTTGCTCCCCACACCAGCCCCCTGCGCGTGTCCACTCTGATCCTGCATCCCACTCCCTGGGAGCCAATA20.149812734082397No Hit
AGGACTTAAGACAAATGGGACTGATGTCATCTTGAGCTCTTCATTTATTTTGACTGTGATTTATTTGGAGT20.149812734082397No Hit
GAGTTGGCATGTCAAATGCATCCTCTAGAAAAATAATTAGTGTTATAGTCTTAAGATTTGTTTTCTAAAGT20.149812734082397No Hit
CCTTTATATTTGGGGTCCTTTTTTATACAGGAAAAACAAAACAAAGAAATAATTGTCTTTTAAAAAAAAAA20.149812734082397No Hit
GTGATAAGGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
CCTATGTACCTGTTACCACTTTCAAAAATCTACAGACAGTCAATGTGGATGAGAACTAATCGCTGATCGTC20.149812734082397No Hit
GGGGACCCACAACAAATAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
CTTTTAAGCATCTTACACACCTCTCACTAAAACTTTTACCTGGAAATGATGTGGAGATAAAGAAATTTCTC20.149812734082397No Hit
TCCCTACACGACGCGGGGGAACAAAACAGAAAAAAGTCTTTGTATAGTAATAAAGTCTGCAGTTCACGCAA20.149812734082397No Hit
GAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
CCCTGCCCCCAGAGCAATAAAGTCAGCTGGCTTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
GTGTTAATAGTGTAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
GCTTCCAACACTACTTCAGGGTGGCAGTGTTTGGGGCACTGGGCGAGCCTGCCGGCCTCTAGATGGCCTCA20.149812734082397No Hit
GGTGTGTGTGTGTCTTTCCTAGGACCCACCATACCCTGTGTATGTATGCATGTTTTTGTAAAAAGGAAGAA20.149812734082397No Hit
ACTCAGAAGAGGTGCTGGGTCACTTTCCGTATCCTGATCCTTCCCAGTGGACAGATGAAGAATTAGGTATC20.149812734082397No Hit
ACTTAATAAATATTACAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
GCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
CCTCTACCCCCTCTAGAGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit
GTGTAATGATTGAATGTATCTAAACTGTAATAATTTGAAACTGACAAACATAACCTTCTCAGACTTACAAA20.149812734082397No Hit
ACTGAAGACAGGCTATTCTCTGGAGAAAAATAAAATGGAAATTGTACTTAAAAAAAAAAAAAAAAAAAAAA20.149812734082397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)