FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15390
Sequences flagged as poor quality0
Sequence length71
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5113.3203378817413904No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.50682261208577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.40935672514619886No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.27290448343079926No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.253411306042885No Hit
ATGCTATGTTGGCTAAAACTGGTGTCCATCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGA360.23391812865497078No Hit
TTCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.17543859649122806No Hit
CGTTATTAGCATATCTTTGTGCTTTATTATCCTGGTGACAGTTACCGTTCTATGTAGGCTGTGACTTGCGC260.16894087069525668No Hit
CAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCGTG250.16244314489928524No Hit
AAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA240.15594541910331383No Hit
GACTCACTCAGGTTTGAGTGTTCAATTGCTCTGGAGACAGAGGTTGGAGGAAGCAGGCTAGACCAGAGACC240.15594541910331383No Hit
TTCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.14944769330734242No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT230.14944769330734242No Hit
TCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACAT220.142949967511371No Hit
GCAAGACAGTGCGCTTCAACGTGCTCAAGGTCACCAAGGCTGCCGGCACCAAGAAGCAGTTCCAGAAGTTC210.13645224171539963No Hit
GATAAACGCAGTATAACCAGATTAAAAGTAAATGAGAAAATTAGGCCGGGCATCGGGGCTCACGCCTGTGG210.13645224171539963No Hit
TTATTGTTCTTATTCCTTCAATAGTTATTACAGTAATTTTCCTCTTCTTCTGGCTTTTCATGAAAGAAACA210.13645224171539963No Hit
ACCATATAGGTCAATTTAGAATTTTAAAAGTTGGAGTTTAGTGTTAATTATGTGTTTTTGACAGCTTTGAC210.13645224171539963No Hit
AGGTTACTGTATACATAGCCTGAGTTTAAAAGGCTGTGCCCACTTCAAGAATGTCATTGTTAGACTTTGAA210.13645224171539963No Hit
ATCGAGACCATCCTGGCTAACGCGGTGAAGCCCCGTCTCTGCTAAAGGTGCAAAAAAATTGGCCGGGCGTG210.13645224171539963No Hit
AAGAAAGATAGCTTTCACAAATTGTCCCCAAAAGAAAGAAAAAAAACCGGAAAAGGGAGAGAATACTTGGA200.1299545159194282No Hit
AACGTGCGGCTCGTGGTGTGAGTGCCAGAAGCTGCCTCCGTGGTTTGGTCACCGTGTGCATCGCACCGTGC200.1299545159194282No Hit
GTTAATATGTATTTAACACTGTTTTAACTTTTATTCTCTATCTGTTCCTTATCCGTATTGCCTAATAGAAC200.1299545159194282No Hit
GACATATCTTCATTAAGAGTTTTTGGAAAACTCATCAAATTCGATGAATACATTTTCTTCATAACCCATTT200.1299545159194282No Hit
CTTACAACTATCTCTTTCCATTTCTTACTATTTGGTTTTCAAATTTCATTTAAATGTCAGAATTTCCTTTT200.1299545159194282No Hit
GCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGA190.12345679012345678No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA190.12345679012345678No Hit
GTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAA190.12345679012345678No Hit
ATATATGATTTTCTTTAAGGATGGTTTAGAAATGGAAAAATGTTAACAAATGTGGCAATTATTTTGGATCT190.12345679012345678No Hit
GGTAGAATTTAAGGTTAATGCACTGGACAGAATAAAGTGGATAGTATGTAATGCTGGCCCAGTAAATTAAC190.12345679012345678No Hit
GGAATATTTTATAAATGTGCATTGAGTATTTTTCGTACTGTGGTATAGGAGTATATTTTGTACTGTTGATG190.12345679012345678No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG190.12345679012345678No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGAAGAGTCT180.11695906432748539No Hit
ACTGGGCACAGCATGCGGAAAATACTACAGAGTGTGCACACTGGCTATCATTGATCCAGGTGACTCTGACA180.11695906432748539No Hit
GGCTGATCCATCTGCCTTTGTGGCTGCTGCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTG180.11695906432748539No Hit
AATGAATATTATCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTCAGAACTGTTT180.11695906432748539No Hit
GATATACACACATATGTGTATGGATGAGAGAGCAACAGAATTAAAACAGAAATCAACATAGTGGATGAGTT180.11695906432748539No Hit
GTGTTGTACTCGTAAAACAAGGATCATCGATGTTGTCTACAATGCATCTAATAACGAGCTGGTTCGTACCA180.11695906432748539No Hit
GGTTTTGGCTGTGTGAGGAAGACGGAAGAGACGGCGGCGGAGGGAAACCGACTTCCACTAGTCCGGGTCGC180.11695906432748539No Hit
GTGGTGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAAAGACTGGTTA170.11046133853151398No Hit
TCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCGTGTGGCAGT170.11046133853151398No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAG170.11046133853151398No Hit
ATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAA170.11046133853151398No Hit
CACCTGGAGACTAAGAAATTAAGTTTTGTTTTGACATCTTCAGTCCTGTGTGCTTTCAGAAAACCATTTTC170.11046133853151398No Hit
GATGAAGATTCACCAAATAAGCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTACA170.11046133853151398No Hit
GCATTACGATTAGGAGGGAGTGAGGGCCAAGTCAGCAAATTATTCTGGAGCAGTTGATTAGCCCTTGTTCT170.11046133853151398No Hit
TCCCTACACGACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11046133853151398No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCACTATTAAAGGTTCGTTTGTTCAA170.11046133853151398No Hit
CCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGCGGATCACGAAGTCAGGAGATTGAGACCATCTTGGC170.11046133853151398No Hit
TCCCTACACGACGCGGGGCTGAATAAACTATTTATTGTTTCTTATTCCTTTGATTTGTATGTAATTAATTT170.11046133853151398No Hit
ATTGTGATGTAGGGATTTGTGAATGAGACATTTACATGTGAAAGGTGACTTCACTAGTTACCTGCTTGAGC170.11046133853151398No Hit
GCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACT170.11046133853151398No Hit
GTTCAGATGCTTAGAATAAAATGTCACTGTAAGATCACAAGCCTGTATGTGGAATGTAGAAAAATAACCAC170.11046133853151398No Hit
CGCGGGGTCATTAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCGCACCACTCAAAATGGCTAT160.10396361273554255No Hit
ATTTAACACTGTTTTAACTTTTATTCTCTATCTGTTCCTTATCCGTATTGCCTAATAGAACAAGAGAAATT160.10396361273554255No Hit
GCTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGTGCAAGGAGCTGGGTATCACCGCCCTACACATCAA160.10396361273554255No Hit
ACCCTGACTAAAGCTTGAAATGCTACATTTCCAAGGTGAAGATGTGTGGGCACATGTTATGGCAGATTGAA160.10396361273554255No Hit
TTCTAATTCTACTGACTATCCTAGAAATCACTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTA160.10396361273554255No Hit
GAGGGGCTGAAAATGGTGGAAAAGGACCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCT160.10396361273554255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)