FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002878996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002878996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39243
Sequences flagged as poor quality0
Sequence length71
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4501.1467013225288587No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.16308641031521545No Hit
CCCTACACTTCATCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT490.12486303289758684Illumina Single End PCR Primer 1 (100% over 54bp)
ACACTTCATCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT490.12486303289758684Illumina Single End PCR Primer 1 (100% over 58bp)
TTCCCTACACTTCATCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT460.1172183574140611Illumina Single End PCR Primer 1 (100% over 52bp)
CTTCATCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAA450.11467013225288586Illumina Single End PCR Primer 1 (100% over 58bp)
TCCCTACACTTCATCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA440.11212190709171063Illumina Single End PCR Primer 1 (100% over 53bp)
CTCTTTCCCTACACTTCATCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG410.10447723160818491Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)