FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168
Sequences flagged as poor quality0
Sequence length71
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40935.01712328767123No Hit
CAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAAT231.9691780821917808No Hit
GTGTTGGTTTTTATTCTGCTTATTTGGTTGCTGAGAAAGTAACTGTGATCACCAAACATAACGATGATGAG221.8835616438356164No Hit
ACTTTTGGGGGATATATTTTGAGAATGATGAAACGGAATAAAATTGTAAAAAACTAAAAAAAAAAAAAAAA221.8835616438356164No Hit
CATCAGAAGTGGTAGGAAAGCCTCACTGATATATTTTCCAGGGCAATCAATGTTCACGCAACTTGAAATTA221.8835616438356164No Hit
GTAATCTGCTTTGTTAATGAAAATGATAAAACAGAATATTGACAAGCTAGGACACCTGTGGTATCTTTAAT201.7123287671232876No Hit
GAAGAATTTTGTCTGACTCCCATCTACCCCCCAAATGGATACAAAACCCAACAACACATCAGCAGAAACTA191.6267123287671232No Hit
GTCCTGCAGCCCTTATTCCCTCCATAGAAAGCATCCTCAGAGCACCTTCCCTGGCTTCCTACTCTGCCCCC171.4554794520547945No Hit
ATAAGTAGCTTCATTTTTCCAAACCCCAGTTTCCTCATCTATAAAAGTAGTAAGGTGCCCAATTCCCAGGG151.2842465753424657No Hit
CAATCTGCATTGATTACAATTTGGATATTTCCTCTCCTCCTGTAGCCATACTTTGATTTTGCTTTAAAAAG151.2842465753424657No Hit
CGTAGGCCTTTTTGGTCACTTGTCCACCATGTCTAATTCTCGACCTAATTTCTTGGGAGTTTTGTTAATAT131.1130136986301369No Hit
GAAGTTGACACATGTGTGTTGGGAGATAATTTTCCACAGATTTCATCTTGTGAGCAAAGGCACTGACTGTC121.0273972602739725No Hit
GGAAACAGCCCCCATGCTCCAATCACTTCCCACCAGACCCCTCACAGGACATGTGGGGATTATGGGAACTA110.9417808219178082No Hit
GAGAGGTCCTTCACTGTGTGTACACAGCAAGAGCATGTGTGTGCCACTTCCCCTACCCCAACGTGAAAACC110.9417808219178082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.7705479452054794No Hit
GTTTTAATCACATTCACTTTACTTGAAATTATGTTGTTACACAACAAATTGTGGGGCCTTCAAATTGTTTT90.7705479452054794No Hit
TCCTACTGATGAAATTCCCACTAAAAAGTCAAAGAAGCATAAAAAGCACAAAAAAAAAAAAAAAAAAAAAA80.684931506849315No Hit
GGTTTGATAAAGAACTTAGGGATTAAAAAAAAGATGGAGTTTTCTAACGTGAGGATGAAGTCTACACTTCA80.684931506849315No Hit
CGGGCTTTCCCCCAGGCGCCTGCGCTAAGTCGCGAGCCAGGTTTAACCGTTGCGTCACCGGGACCCGAGCC70.5993150684931506No Hit
GGGCAAGGATGTTAATTTTGAATTCCCAGAGTTTCAATTGTAAACAAAAATGACTAAATAAAAAGTATATA70.5993150684931506No Hit
CTATTTGTGGGCTATAGGAAACTTTCTGATGCCTCCGGATTGTGTTAGTAGTAGCCATCAGGAGGGTCTCC70.5993150684931506No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG60.5136986301369862No Hit
GGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGTGGGTGGATCACTTGAGGTCAGGAGTTCGAGACCAGCC60.5136986301369862No Hit
CTATAAATGATTGTCTTTAATAAATATGTGATAAAATCCAATTTTTATTATTTTAAAAAAAAAAAAAAAAA60.5136986301369862No Hit
TCCCTACACGACGCGGGACAGAAAATACTTGCTTTAATAATATATATGCTGTTGATTTCACAAAAATTTAT60.5136986301369862No Hit
GCACTCCAGCCTGAGTGACAGAGCGAGACCCTGTCTCAAAAAACGAAAAAAAAAAAAAAAAAAAAAAAAAA60.5136986301369862No Hit
AGCTGAACCTGTTCAAAATGTTAATAAAGGTTTCGTTGCATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA50.4280821917808219No Hit
ACAGTTTTTCTTTTGAATTTAGTATTTGAGATGAGTTGTTGGGACATGCAAATAAAATGAAGAATGGAAAA50.4280821917808219No Hit
GATTATAGGCGTGAGCCACATGCCCAGTTGATAATATTTTTAATATACTGTTTTAGGTTAAAATATTTGAA50.4280821917808219No Hit
AAACATGTATTTTCAATAAATTGAGATTCCCAGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3424657534246575No Hit
AATAAAAACATATTTTATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3424657534246575No Hit
GCAGTGGCCATGGATCTGTTCCTCTGTGCTAAATGTCTTGTGGCAGCGTGTGTTTGTATGTCTGTGTATGT40.3424657534246575No Hit
TACCCACACCCACCCAAGAACAGGGTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3424657534246575No Hit
GAGGTACTATCTTGTCCTATACACTTCTACTTGGTCACTTTGCTTTCTTCGTTAATGGAACATACAGTAGC40.3424657534246575No Hit
GCTTAGAGCTTGGAACAAGGGGGAATAAACCCAGAAAATACAGTTAAAAAAAAAAAAAAAAAAAAAAAAAA40.3424657534246575No Hit
GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3424657534246575No Hit
GTGGCTCACACCTGTAATCTCAGCACTTTGGGAGGATCACTTGAGCCCAGGAGTTCGAGACCAGCCTAAAC40.3424657534246575No Hit
CTTCCGATCTAACCAAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2568493150684931No Hit
GGGGCGTAATAAAGATGACCTGGACCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2568493150684931No Hit
CGTGGCCCAGATTTATGGTGAGTCCCAGGAACTGGGCGCATGGAGGAGGTGGCTCTGGGAGGGAGGCCTTC30.2568493150684931No Hit
GCTCATGGAGCTTCATGGTGAAGGCAGTAGTTCTGGAAAAGCCACTGGGGACGAGACAGGTGCTAAAGTTG30.2568493150684931No Hit
GACTCTCTTTTCGGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCCACGTTGCTCACAAAAAAAAAA30.2568493150684931No Hit
GGGCCAAACGTGCCTGCTTCCTGTTTTTGTAAATAAAGTTTTGTTGGAACACAAAAAAAAAAAAAAAAAAA30.2568493150684931No Hit
AGTTAATACCAAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2568493150684931No Hit
GTGGATCTGTAATAGCATATTGTAGATGCACTTTGCAGCAGTTGGAAAAGAAAGTGTTGTGTGATTTGATT30.2568493150684931No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTT30.2568493150684931No Hit
GACTCTCTTTTCGGACTCAGCCCACCTGCACCCAGGTGAAATAAACAGCCACGATGCTCACAAAAAAAAAA30.2568493150684931No Hit
GACTTCCCAGGCGCTCAGGCCTGGATCTTGCTCCTCTGTGAGGAACAAGGGTGCCTAATAAATACATTTCT30.2568493150684931No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA20.17123287671232876No Hit
AAACAGCCTTGTTGCTCACACAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
AGTTTTTAAAATATACTTATTTCTAAGTATTTATTTCAGCATTTATGAATTTACAACATTGGCAAGTGATT20.17123287671232876No Hit
CTCTAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
CTTTCAATCCGTTTGATACCATTTGGCTCCTTTTTTGGCAGAACAGTCACTGTCCTTGTAAAGTTTTTTAG20.17123287671232876No Hit
GGAAACAGCCCCCATGCTCCAATCACTTCCCACCAGCCCCCTCACAGGACATGTGGGGATTATGGGAACTA20.17123287671232876No Hit
GCTCTTTTCAGTTTTTGCTTATACACAATTCATTCTTTGCAGCTAATTAAGCCGAAGAAGCCTGGGAATCA20.17123287671232876No Hit
CGGGCTTTCCCCCAGGCGCCTGCGCTAAGTCGCGAGCCAGGTTTAACCGTTGCGTCACCGGGACCCGCGCC20.17123287671232876No Hit
GTGGATCTGTAATAGCATATTGTAGATGCACTTTGCAGCAGTTGGAAAAGAAAGTGTTGTGTGATTTTATT20.17123287671232876No Hit
CGGCCTGGTGGGCGAGATCATCAAGCGCTTCGAGCAGAAGGGATTCCGCCTCGTGGCCATGAAGTTCCTCC20.17123287671232876No Hit
GCATATATTTTTATATTTACTAAAATGGACTGTGGTGACTTTGGATAATAAGGAAAAGTTTAATATTAAAA20.17123287671232876No Hit
GTTCTTTGATTTGTACCATTCCTTCAAATAAAGAAATTTGGTACCCAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
TAAAGAATCCTTTTGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
GGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGTGGGTGGATCACTTGAGGGCAGGAGTTCGAGACCAGCC20.17123287671232876No Hit
GCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGC20.17123287671232876No Hit
CCACGAGTACGGGGTGTGGCCATGAATCCTGTGGAGCATCCTTTTGGAGGTGGCAACCACCAGCACATCGG20.17123287671232876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA20.17123287671232876No Hit
ATTACACAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
TCCCTACACGACGCGGGGACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
GGAGGAAGGCGAGTTTTCAGAGGCCCGTGAAGATATGGCTGCCCTTGAGAAGGATTATGAGGAGGTTGGTG20.17123287671232876No Hit
TCATATACCATGATATAATCTGAAAGGGGCAGATTAAAAAAAAAAAAAGAATCTAAACCTTACATGTGTAA20.17123287671232876No Hit
GGATCCCGCCTGAAACGGACCTGCAGAGCAGCAGCACCCTTCCGGTGTGGAGGCTATGTAGCTGGTGCGCT20.17123287671232876No Hit
GGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGTGGGTGGATCACTTGAGGGCAGGAGTGCGAGACCAGCC20.17123287671232876No Hit
GAACTATGTAAACAAAAAAGCTTTTGTTTTGCTTACAAAGTATATTTAAGGATTATTCTGCTGAAGATTCA20.17123287671232876No Hit
CATGTAAATAATACATTGTTCTAAGTCTTTTTCATTAAAAATTTAAAACTTTTCCCATAAACTCTATACTT20.17123287671232876No Hit
ATGTAACCATATGGAAGCAACTTTTTTTGGTGTCTCAAAGGAGTAACTGCAGCTTGGTTTGAAATTTGTAC20.17123287671232876No Hit
TACTTGTACCTGTCTGGATTATTCATTATTACATGAAGTATCATGTTTCTGAAAAACCATATGGCATAGTT20.17123287671232876No Hit
CTCATAGTCCTAGCCAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
GCACTAAATTACTCAAAATGTGCTGTAAAGTTTCTTCTTTCCAGTAAAGACTAGCCATTGCATTGGCTCCT20.17123287671232876No Hit
GGGCTCTGCCCTCTTGAAAGGCATTTACTTGTTTAACACTTGTCCAGCTACAGTGGGGTACAGTAGCTGGC20.17123287671232876No Hit
CCTTGTGAACTGCTCAGTAAACTCTACATTGTTCATGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
AGCCTGGGAATCAAGTTTGAAACAAAGATTAATAAAGTTCTTTGCCTAGTAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.17123287671232876No Hit
CGAGTGAGGAATAAAATGGATGTAAACATTTACATGGGATGCATTAGAATTCTGCTGTGTGTACTGTCTTT20.17123287671232876No Hit
GAAGTTGACACATGTGTGTTGGGAGATAATTTTCCACAGATTTCATCTTGTGAGCAAAGGCACTGCCTGTC20.17123287671232876No Hit
GGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGTGGGTGGATCACTTGAGGTCAGGAGTTCGAGACAAGCC20.17123287671232876No Hit
TAGTTGAAGTCTGTGGATGCAGCTGTTATGAAGATGGTTAAACTTGAAACAAACAATTTTAAGAATTATTT20.17123287671232876No Hit
GTAGCTCAGGAGAGCACCCCTCCACCCCATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCTGCAGT20.17123287671232876No Hit
GGACATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17123287671232876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)