FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4043
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1974.872619342072718No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1573.8832550086569375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG481.1872372000989364No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA390.964630225080386No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA310.7667573583972298No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT240.5936186000494682No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG180.4452139500371012No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC100.2473410833539451No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCC100.2473410833539451No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGC90.2226069750185506No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA90.2226069750185506No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTA80.19787286668315607No Hit
CACCATTGGCTCGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGATTTGGACCGGAACAAGGACC80.19787286668315607No Hit
AGTTGTTACAGAGGTTTCCTAACAAACCTTACCTCAGCTGAAAATGGACAAGTATTTTCAATGACTGAAAT80.19787286668315607No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC70.17313875834776157No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC70.17313875834776157No Hit
GCTAGTTGCAAAGCTGTATTATCAGAGTAAAAGTGTATTTGTAAACTGTATGGGAACTAAAAATTAGGAAT70.17313875834776157No Hit
CTGTAGAGAAATGCAAACCTCGACTCTCAAGGATGTGAGGAACACAAGTTCATTTCTGTTGTTGCGGAGAC70.17313875834776157No Hit
GATGAAAAATTATAACCAAGCATAATATAACAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTA70.17313875834776157No Hit
GCTCTGTCTGAGATTTTTAAAAATCAGCTTAATGAGAGTAATCTGCAGACAATTGATAATAACATTTTGAA70.17313875834776157No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGG70.17313875834776157No Hit
GGATGAAAGTCCTTGTCCAGCTCACGCCTACTAGAAATCAAGCCAAAGTCTATGCTCGCACAGGATTTGAA70.17313875834776157No Hit
CAGTGGACTGTCTTTTGGATTCAAAGTGGGCAAAGGCCAAGAAAGGAGAGGAAGCTTTATTTACAACCAGG60.14840465001236705No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCA60.14840465001236705No Hit
GTGTTTCATTTGTGTGATTTCGTACCAAAAAAATGTGTTTGAACTATATTGTATGTAATTTGGAAGTCGTG60.14840465001236705No Hit
CCATGGTGGTGTAGGAATTTCTGTGCAACACCTCACCACGTCTGGTTAATCCTTGCACACTTCAGTGTTTC60.14840465001236705No Hit
CCCTAAGGGTGAAGGAGAACGACCTGCTCAGAATGAGAAGAGGAAGGAGAAAAACATAAAAGGAGGAGGCA60.14840465001236705No Hit
CTGCCTGCACCCCAAGCTGCAGAGCACCAAGCGCTTCATCAAGTGGTACAACGCCTGGAACGAGAAGCGCA60.14840465001236705No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAA60.14840465001236705No Hit
GCTCCTATTTTAAATGTTTTGAAACATGCTAAATATTCTTTCCTAATTATTTTATCACTTATACTACCTTT60.14840465001236705No Hit
GAGTTCGAGAGATCCAAGAGGAAGAATCCAGCTGCCTATGAAAATGACAAATGAGCAACGCATCCGGATGA60.14840465001236705No Hit
GCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGG60.14840465001236705No Hit
GACCAAGATTACATTCTCAATTAGAAAACTGCAATTTGGTTCCACCACATCCTGACTACTACCGTATAGTT60.14840465001236705No Hit
GGTACAGGTTGTTTAACGTTAGCAATATAGCTTCTGTAAACTGCAAATCAATAGCAGATTTTAACCCTAGG60.14840465001236705No Hit
CACGACGCGGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTC60.14840465001236705No Hit
CCCTCATTCACACGAGAAAACACCCTCATGTTCATACACCTATCCCCCATTCTCCTCCTATCCCTCAACCC60.14840465001236705No Hit
CTTGGTAAATACGGCATCATCTGCATGGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAA60.14840465001236705No Hit
AGAGCATGTCTTTACTTGAAAAACTCTTGATCAAGAATTTGGGTGGGAGAAAAGAAAGTGGGTTATCAAGG60.14840465001236705No Hit
GCCTTCTGTACTTGAAGTTGATTTGAAATATGTAAGAATTGATGATGTATTTGCAAACATTAATGTGAAAT60.14840465001236705No Hit
GGTATTATAACAATCATTGGAAAGCATGGAGAGCTACATTTCATAATTTGAGGGAAAATTTCTAACAGATG60.14840465001236705No Hit
CAGGCATCGGATTATTTCTTCTGTAAATATTTCAGAATGTATCTCTCCAAGATGAGAGCTCATTAAAAGAC50.12367054167697256No Hit
GCATCATTTCCTTACAATACTGTCCGGTGGACAAAATGACAATTGATATGTTTTTCTGATATAATTACTTT50.12367054167697256No Hit
GAAGACATTCCAGGAGCTAGCAATTTTAAGAGGTGTCCCTCCAAAGTGACCTGATGGAAGTCCTGAACTTG50.12367054167697256No Hit
CCCCATCGTCTCAGCCCAAAATCACCTTAAGCTGATAAGCAACTTCATCAAATCTCAGGATCCTAAATCAA50.12367054167697256No Hit
CTCAGTGCAGACAAAAAGTGCATCATTGTAGAAGAAGGCAAAGAGATCTTGGTTGGAGATGTTGGTGTAAC50.12367054167697256No Hit
GAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAAATCTAGAGTA50.12367054167697256No Hit
GGAGAAGAAGCCTGCTGCATAAACTCTTAAATTTGATTATTCCATAAAGGTCAAATCAATTTGGACAGCTT50.12367054167697256No Hit
GGTTAGAGAAAAAAGAATAAAAAGAAATGAGCAAAGCCTCCAAGAAATATGGGACTATGTGAAAAGACCAA50.12367054167697256No Hit
GATTATAGAAGAAGGGAAATAGCAAATGGACACAAATTACAAATGTGTGTGCGTGGGACGAAGACATCTTT50.12367054167697256No Hit
CCTGAATGTTCTATGTAATGTAAAATATTCTTTTCTTGCTTTCTTGTGTTAAGGTATATATTCTATTTGTA50.12367054167697256No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAAT50.12367054167697256No Hit
GACCATGGAGGAGAAGAAAGCATTCATGGGACCACTGAAGAAAGACCGAATTGCAAAGGAAGAAGGAGCTT50.12367054167697256No Hit
CCAGCACCTCCCTTGCTATCCAAGGGTTGGGATTTTTAAAAAGAAAAACAAATTTTAAAAAAAAAAAAAAA50.12367054167697256No Hit
ATGCTAATATGATGGTCTTAGGACATGAACCAAGACAAACAACTTCGAATCTGGGTCACTTAAACAAGCCA50.12367054167697256No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT50.12367054167697256No Hit
GGCAGTGGTCGGCACAGCCTGCAGACAGCTGGGCGGCCACACGTAGCAGCGGTGCACTAACGTGTGCAGAA50.12367054167697256No Hit
GCTGGGACTGGGTTCATTCTCATGACTTGGGGCTGTCGAGATTTAAAGTGATGTAAGCTGTGGTTATGTGG50.12367054167697256No Hit
AAGTGACTCAGCTTCTGGCTTCTCTGCTACATCAAATATCTTGTTTAATGGGGCAGATATGCATTAAATAG50.12367054167697256No Hit
CCCCTCACTCCTCCCCTCCTTCGCTCGCTCAACCTCTTTTGTTCAGTATGTGTAACTTGAAGCTAATTTGT50.12367054167697256No Hit
AAAAGCTTTTTGATTTCCCAAAGGAGGGACATACCACTATATCAGATAAGCTTGACATTACAGCCAAGATG50.12367054167697256No Hit
CTCTTCAGACTCCGTACTGAAAACAGAAGACCCTTCCAGAAGGGGGTGAGTGTTGCCTCTTCCTGTGTTCC50.12367054167697256No Hit
GACTCCATGAATATCAAAGCCCATATCCACATGTTGCTAGAGGGGCTTAGAGAACTACAAGGCCTGCAGAA50.12367054167697256No Hit
GAAGGGATGGGTGATACTTCTTGCTTCTCATGATACATGTTTCTGTATGTTAATGACTTGTTGGGTAGCTA50.12367054167697256No Hit
CCCACATGTGTGCTGACAGGGAAGTGTACAGCAGTAAGTGCTCACTTTTGAAATGAGGAAGGTTCTCAGAT50.12367054167697256No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT50.12367054167697256No Hit
GCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAG50.12367054167697256No Hit
GCATGCAGCTCTTGAGGCAGAGAATTCCAAAGGAGAGGTAGAGACCCTAAAAGCAAAAATAGAAGGGATGA50.12367054167697256No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCC50.12367054167697256No Hit
CTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAAAC50.12367054167697256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)