FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17379
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3031.7434835145865701No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1100.6329478105759825No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT360.20714655618850336No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC310.17837620116232233No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA260.14960584613614133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260.14960584613614133No Hit
TTCCTATTTAGAGATGGTGACATTCTTGGAAAGTACGTAGACTGAAATAAGTCACTATTGAAATGGCATCA260.14960584613614133No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGC240.1380977041256689No Hit
GTCCTTAACATCTACCTCTCTGAATTTTCATGAATTTCTATTTCACAAGGGTAATTGTTTTATATACACTG230.1323436331204327No Hit
GTTTCACGTAGTTGGGTTGACAAGTTTCTGCCTTTAAGATAAATGAGTAATAGTCTAATGTCCAGCTCAGC220.1265895621151965No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC220.1265895621151965No Hit
AGTCAAGACAGTGGTTCAGTTGGAAGGTGACAATAAACTGGTGACAGCTTTCAAAAACATCAAGTCTGTGA220.1265895621151965No Hit
CACACACACTGGAAAGTGAGGGTGGGGGCTCTGCAGTCAGACAAACCTAAGATCACATCCTGGACAGGCCA210.1208354911099603No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGAAATTTGTTATAATGAATGAAA210.1208354911099603No Hit
GTCTGTGACCGAACTCAACGGCGACATAATCACCAATACCATGACATTGGGTGACATTGTCTTCAAGAGAA210.1208354911099603No Hit
GTATAAAGCAGTTAATCAGATTTGAGGACAAGGGAGATGGGAGAAATAAACTGGATGTAGTTTTGACAGAC200.1150814201047241No Hit
TGTCTGGTGTACTTTTTCATGAAAAGGAAAAATGGTTAACATGTTTACACAAGAAAAAAAGTCAAAATTAT200.1150814201047241No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG200.1150814201047241No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC190.1093273490994879No Hit
TCTAAAAAGACTGCAATGGCTGCTGCTAAGGCACCTACAAAGGCAGCACCTAAGCAAAAGATTGTGAAGCC190.1093273490994879No Hit
ATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGG190.1093273490994879No Hit
GTTTGCACCATCAGCCAGCAACCTTTCTGGGAAGCGTGCTTATTATGTACCAACATAAAAGCTTGAATATA180.10357327809425168No Hit
TCACTGAAGATAATACCTGGAAGCATCATAGTGGTTTCTTAACTCTCCAAAATAAGATTTCTTCTCTGTAG180.10357327809425168No Hit
GCTAAAGAGACTGCCCAGAAAGCAGTTAACAGTGCCACCGGTGTTCCAACTGTTTAAAATTGATCAGGGAC180.10357327809425168No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGC180.10357327809425168No Hit
ACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG180.10357327809425168No Hit
ATTAACAATGTAACCATATGGAAGCAACTTTTTTTGGTGTCTCAAAGGAGTAACTGCAGCTTGGTTTGAAA180.10357327809425168No Hit
ATACAGAACAGATTACAAATGAGAAGCTGGCTATGGTTAAAGCGGAACCAGATGTTAAAAAATTAGAAGAC180.10357327809425168No Hit
CTTCCAATTTACATACTCTGCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGAAA180.10357327809425168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)