FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21319.017857142857142No Hit
AAACCAATGAGTGCTGCATTTTGGATATTTAGTTTTATCTTTAGTTCAACACCATCATGGTGGATTCATTT110.9821428571428571No Hit
GGAAAAGTGAGAGGTCAGACTCCTAAGGTGGCCAAACAGGAGAAGAAGAAGAAGAAGACAGGTCGGGCTAA90.8035714285714285No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.625No Hit
GATGAACTTCTTATTCAGCAAATACTTGAATATTGTGCTAGGCACTGGGGTGACAGTCGTGAGCAAAATGA70.625No Hit
GCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGCTTCAAAATCCTCAAGGCAGAATGTTTAGCA60.5357142857142857No Hit
AGTGAACACACTGATTAGGTTATGGTTTAATGTTACAACAACTATTTTTTAAGAAAAACAAGTTTTAGAAA60.5357142857142857No Hit
GCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAGGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCT60.5357142857142857No Hit
GAAACGGCGTCATGCCCAGCCACTTCAGCCGAGGCTCCAAGAGTGTGGCCCGCCGGGTCCTCCAAGCCCTG60.5357142857142857No Hit
GGCGTGAGCCACCGCACCCGGCTAGTTTGTCTTTTTTAAAGGTTACTAAGATGCATTTATTTATAAGCTTG60.5357142857142857No Hit
CCCCATGTTATGGCTGATCAGTCATCCACCAGGAACGAAGATTTCCTGAAGAAGACCTGGTCCCTCTGGAG50.4464285714285714No Hit
GCTCTGGGTTGGCATCTACGTGTGAGCACCCAAGGGTAACAACCAGATGGCTTCACTGAAACCTGCTTTTG50.4464285714285714No Hit
GTATTGGGAGGAGTGTAGAGATTTTTTTAAAAATAGTATTATAGACAAAATTGCCATTAATTTTTCACAAG50.4464285714285714No Hit
GCAGCATGGACTGCGTGCCTCTCCGCCGGAGGTCTAGCTGTGGGCGGCCAAGAAGGGTCACCCTTGAGGAC50.4464285714285714No Hit
GTAGAGTTCTTCCACAAACAGAACAAGATGCTAAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACATT50.4464285714285714No Hit
CCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAG50.4464285714285714No Hit
CGTTTGCAAGACGAGTTGTACTGCTGTCACAGGAAATGGACGCTGGATTACAAGCATGGCAGCTCAGGCAG50.4464285714285714No Hit
GTTAAACTCTTGTTAGAAGACAGTGGTGTCCTCAAAATTTTACTTTGTAATTCTTCAGAATTGATTATTTT50.4464285714285714No Hit
GGGCAGAGGCCACCGTGTGTGTCCCGAGTAACTGTGCCGTTGTCGTGTGATGCCATAAGCGTCTGTGCGTG50.4464285714285714No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG40.35714285714285715No Hit
GCTTCATGGTGAAGGCAGTAGTTCTGGAAAAGCCACTGGGGACGAGACAGGTGCTAAAGTTGAACGAGCTG40.35714285714285715No Hit
CCCTACAGAGGTGGTGAGCATGTAACATGAGCTTATTGAGACCATCATAGAGATCGATTCTTGTATATTGA40.35714285714285715No Hit
GTGGTAGGTTAATGTGTTCAGAAGTCGCTGTCCTTACTACTTTTGCGGAAGTATGGAAGTCACAACTACAC40.35714285714285715No Hit
GAGTTGACCCTTATGTCTAGTTTCTTGGTTACCGTTGAGCTGTTGTTATTTGTAAAGCTATTAATTTGAAT40.35714285714285715No Hit
GCTTACTCTGAGGCTTGGCACTGGGGGCCAGGGCCTGCCCCAGGGCTCCTGGAATTTCCCTTGATCCAGCT40.35714285714285715No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACC40.35714285714285715No Hit
ACGCGGGCTCCAAAGCAGAGGTAGAATGTTCAGGTTTCACCATGGATTTTCTACTTATTTCGTTTTTGGAA40.35714285714285715No Hit
GCTGTGACTAATCCAATATACTCAGAATGTTATCCATCTAAAGCATTTTTCATATCTCAACTAAGATAACT40.35714285714285715No Hit
CCTGCGTTACAATCACTTAAAATAGAGCAAGCATCCCCTTAGGCTCTTAATTGTAGTTTAAATTCCAGTAC40.35714285714285715No Hit
CTGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACATCAACAGTGGTGCTGTGGAATGCCCAG30.26785714285714285No Hit
GGTGTCCCCCAAGGACTACTCCACCAAGTACCGCTACTGAGGCGCCCTCAGTCTGCGCGGATAAATGTCGT30.26785714285714285No Hit
GAGACGAGGGGTGATCTGTGAGTCCCATGTAAACTTTGTACATTGGAATATTTATGTTTGTGTACATATTT30.26785714285714285No Hit
GGCTTCACATTTCTGGGACTCAGCATTATCCAAAATATCTATTAAGAGCCATACACCATTCTAGCTGCAAT30.26785714285714285No Hit
AATAAGAATACATTGAGGAGTAAACTAACATTCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.26785714285714285No Hit
TTCCCTACACGACGCGGGGATTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.26785714285714285No Hit
GTCTATTAAACCCTTGATATGCCCCCTAGGCCTAGTGGTGATCCCAGTGCTCTACTGGGGGATGAGAGAAA30.26785714285714285No Hit
ATGGTGTGAACCCGGGAAGCGGAGCTTGCAGTGAGCCGAGATTGCGCCACTGCAGTCCGCAGTCCGGCCTG30.26785714285714285No Hit
GGATCTCACTCTGTTGCCTAGACTATAGTACAGTGGCATGGTAATAGCTCACTGTAACCTCAAACTCCTGA30.26785714285714285No Hit
GAACTAAATTTTTTAAACTTTATTTGCTGTTAAATTCTGTGAAGTTTCAGTTATCTAAAATAAATATACAC30.26785714285714285No Hit
GATTTTAATTATTGGAATGCACAATTTTTTTAATATGCAAATAAAAAGTTTAAAAACTAAAAAAAAAAAAA30.26785714285714285No Hit
ACTCAGGAGGCTGAGGCTGAAGGATAACCTGAGCCCAGGAGTTGGAGGTTACAGTAGCTTATGATTGTGCC30.26785714285714285No Hit
TTTATAAATAAAGTTTGCATTAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.26785714285714285No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA30.26785714285714285No Hit
GCTATCAACCCATTGTCAGATCAGCATCAGGCTGTTATCAAGTCTGTTGGTGCTAAAAAGTAAAAGATGAA30.26785714285714285No Hit
GTTTCATTCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG30.26785714285714285No Hit
GTTCTGGTTGCTGGATTTGGTCGCAAAGGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGT30.26785714285714285No Hit
GCCCTAAGTTTGTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.26785714285714285No Hit
GGACGGGAAAGGGAACAAAATAAAGTGAAATCCAATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.26785714285714285No Hit
TCTTTTCACCATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
TTCCCTACACGACGCGGGGAGCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA20.17857142857142858No Hit
CGTGGATAGCTGTGATTGGTGAGTCAACCGTCTGTGGCTACCAGTTACACTGAGATTGTAACAGCATTTTT20.17857142857142858No Hit
GAATAAAAAAGATTCTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
TCCCTACACGACGCGGGAGATTAGACTGAACCGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GTGTTATGTAGTGCTTAATAAAAATAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GGAGATTGAGTTCATTCACCAGTGTCCAGTGATTTACTTACTCAATCATGCCTACGTAGTGAAATCTACAT20.17857142857142858No Hit
CTTATGCCTTGGTCAGAGTAATAACTGAAGGAGTTGCTTATCTTGGCTTTCGAGTCTGAATTTAAAACTAC20.17857142857142858No Hit
CTTCCTAACTGCATGTTGAAAAATAAGCCGTTATTGATCTTAAACATCGGTCAGATGAGTCATACATTGGG20.17857142857142858No Hit
GGCTTATTAGAAGAATGAACTAAGGTGTCTACCATGATTATTTTTCTAAGCTGGTTGGTTAATAAACAGTA20.17857142857142858No Hit
CCACCACTTTATAACCAGATGGTTCCTTTCACAACCCTGGGTCAAAAAGAGAATAATTTGGCCTATAATGT20.17857142857142858No Hit
GATTGTGTTTGTTCATAATAAAAGTGAAGTGAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GTTGAAGGCACAAGCACAGAGTCAGAAAGCTAAATAAAAAAATGAAACTTTTTTGAGTAATAAAAATGAAA20.17857142857142858No Hit
CTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGA20.17857142857142858No Hit
ATCTTAGGTAGTCATGCTGTGCATTTTTTTTTTCATTGGTGTACTGTGTTTGATTTGTCTCATATATTTGG20.17857142857142858No Hit
GGCTGCAAGGCTGGTGTGTCTCCGGACACTACCTTCTAGGGTTTTCCACCCAGCTTTCACCAAGGCCTCCC20.17857142857142858No Hit
TTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTG20.17857142857142858No Hit
GTCATCGCTCGAGACCGGGCCCTGGAGGGCCGCTGACCCTGCCGGAGATAAAGGATACAGAGAGCCCCTCA20.17857142857142858No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA20.17857142857142858No Hit
ATTAACTAGAGACCCTCCAGCCCCCAAGGGGAGGATTTGGGCAGAACCTGAGGTTTTGCCATCCACAATCC20.17857142857142858No Hit
TTCTAGACCTGTTTTGCTTTTGTAACTTGAAGATATTTATTCTGGGTTTTGTAGCATTTTTATTAATATGG20.17857142857142858No Hit
AACTTGGACAGTCTGGACAATAGAATAAATGAAGTCAATAAAACCATTCTTTTAACAAACTTAAAAAAAAA20.17857142857142858No Hit
GTATTTTAGTATATAGTATAAAATGGAATTATTGGGTTAAAGTACTTTTATTACATTTCTTAATCCATTAT20.17857142857142858No Hit
GTTCTGCAGAGCTAGTTGAGAACTCAACATTAATCATTTTAAAAAGTACTGTCCTTGAAATAGATTTGCTG20.17857142857142858No Hit
CAATGAAATTATTAGAAGATTGGATACAAGAGAAAAAAACTTAAAGACACATCAATAGAAATGATCCAATC20.17857142857142858No Hit
AGCTAAGGTTGAAGCCAAGGAAGAGTCGGAGGAGTCGGACGAGGATATGGGATTTGGTCTCTTTGACTAAT20.17857142857142858No Hit
CGACGCGGGGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
CCTTATGTACAGAAATCACATGAAAAATAAAGGTTCCATAATGCATAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
CAATAAAGACTTGTTGAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
CTCGTCCGGGTGTGCCCCAAATAAACTCAGGAACGCCCCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
TCCCTACACGACGCGGGAATGGTCTCTCTTTGGCTGGAATTACAAAACTCAGAGAAATGTGTCATCAGGAG20.17857142857142858No Hit
GCCTTAGACAAGCGCCAAGCCCATCTTTGTGTGCTTGCATCCAACTGTGATGAGCCTATGTATGTCAAGTT20.17857142857142858No Hit
GGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAA20.17857142857142858No Hit
TTCCCTACACGACGCGGGCACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
TTCGAGCCACTGCACTCCAGCCCAACGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAGAAAAAAAAAAAA20.17857142857142858No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT20.17857142857142858No Hit
CACTGATGGTGTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
CCACAGGAGCTGTCACCTCCTGCGGATGGGCAAATAAATTGGTGGAGGACGGAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
CCAGCACTCCAGCCTGGGTGACAGAGTGAGACTCTGCCTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
TTCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GTTCTAGACCAGCCTGGCCAATGTGGTGAAACCCTGTCTCTACTAAAAACACAAAAATTAGGTGGGTATGG20.17857142857142858No Hit
TTCCCTACACGACGCGGGATGCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GGTGGAACCGCCATCAATAAATCAAGCGTGATCAGGGTGAGGAACAGGAAAGAAGGAAATTTGGGGAGATG20.17857142857142858No Hit
GGCAGAAAAAAACCTGTGTACAAGTGGACCTGTGCATTTCAAGCCCCTTTTGTTCAAGGGTCAGCTGTATT20.17857142857142858No Hit
CCCCATGTTATGGCTGATCAGTCATCCACCAGGAACGAAGATTTCCTGAAGAAGACCTGGTCACTCTGGAG20.17857142857142858No Hit
GCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTTTAGAAAAAAAAAAAA20.17857142857142858No Hit
AATAGGTATCTTAATCAAGAATTAAGCTTGCAACATTGGCTTTGCTCAGATGCAGATGGAAGTGTGATCAC20.17857142857142858No Hit
AATAAAGCCTTTTTACACATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GACTACCCCTGGCTCCTCTCACCTTGTCCCTCCACTGCCAACAGAAAACAAAGCAGCTGACTGAGATGGTC20.17857142857142858No Hit
ATTTCATTCATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
TCCCTACACGACGCGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAGGATC20.17857142857142858No Hit
GAAACAGCTGGACGACCTGAAGGTGGAGCTGTCCCAGCTGCGCGTCGCCAAAGTGACAGGCGGTGCGGCCT20.17857142857142858No Hit
CTATGATGGTGACGATTTTTAAAATCAGGAAATAAAAGATCTTGACTCTAAAAAAAAAAAAAAAAAAAAAA20.17857142857142858No Hit
GGCAGAAAAAAACCTGTGTACAAGTGGACCTGTGCATTTCAAGCCCCTGTTGTTCAAGGGTCAGCTGTATT20.17857142857142858No Hit
TGCACAGTCCTTCTGAGATTGTTAAGCAGATTACTTCCATCAGTATTGAGCCAGGAGTTGAGGTGGAAGTC20.17857142857142858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)