FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46005
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.7216606890555375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1730.3760460819476144No Hit
GTTTTCTATTCTAGTTGTAATGATGCCATGGAAATGACCAAATGTCTCTCAAATATGAGAGAGGAGAAAAA980.21302032387783937No Hit
CGTTCGTAGATTCAGTTACATCCATCTGTCATCTGAAGAAGGAGGAAAAAACCCAACATTTCTTGGACCAA940.20432561678078467No Hit
GAACTACAGGAGTCATGGGAAAGAAAATTCTGGCTTCACTACTGCTCACTGCTCACTTTCTGATCACTCTG890.19345723290946637No Hit
ATTTAGAGGAAGGCAAATCAAGGTGATCCCAAAACGAACCAACAGACCAGGCATCAGCACAACAGACCGGG890.19345723290946637No Hit
ACTTAAGGCTGCTTGGTACTGAGTATACTTAAATATAACTCCAGAATCCAGGGACTTGGTGTTAAAACAGG860.18693620258667537No Hit
TTTGTATACCGTTCAGTATAAAAGTGTCCTAAGCATATTAGCCAATCTTTTCACAGTAGAGCATACTTAAG740.16085208129551137No Hit
TTATAGCAGTTACCTGTTACCAACCTGGACAGCCTGAGACAACATGAATAAGATTCCATCAATTCTCAGAT740.16085208129551137No Hit
GTATAAAAGTGTCCTAAGCATATTAGCCAATCTTTTCACAGTAGAGCATACTTAAGGCTGCTTGGTACTGA710.15433105097272035No Hit
AACTCACATAGTCTCACAGACTTGTAATCAGGATGCCAGAATATTAGCCATGACCCCAGAATCTATATATT690.149983697424193No Hit
TCTTTGTGTAGGTTGTTCAACAGTGTTGTGCCAGCCTACAGGAGGAAAGGCCAGACTCACAGAAGGGTGTT680.14781002064992935No Hit
CTTCAATGCTATTCAGAACTACTCAAAGGAAGAAAGAAAAAAACACCAGACTTTTAGACACCCAGGGCAGT660.143462667101402No Hit
GTGTATGGAAGAATTAGACCACACCCCAGACACAAGCTTTGAAAGAGATGTGGTGTGTGAAGACGAAGATG650.14128899032713835No Hit
GTGTGTGAGTGTGTGAAGCCGCCAGTTCATCTTTTTATATGGGGTTGTTGTCTCATTTTGGTCTGTTTTGG630.13694163677861101No Hit
ATGCTGGTTTACCACAGAAAGTTGCTGAAAAACTAGATGAAATTTACGTTGCAGGGCTAGTTGCACATAGT620.13476796000434735No Hit
ACCTAATATCACTTAAATCGAGGCAGAAAGATACAGACATTCAATGAATTATATTCAATGCATTTAAAATA610.13259428323008368No Hit
CTCCAGAATCCAGGGACTTGGTGTTAAAACAGGATTAGAGCATGTAAAGGTACATCTAGATTCATATTTGA600.13042060645582002No Hit
GATAGAGCATGGATGTGGTCAGCCAGTGATTTCTCTGACGGTGATGCCAAACTAGAGCGGTTGGCAGCAAA570.12389957613302902No Hit
GTATAAGGAATTATGTGTGTGTGAGTTGGGATGTATGGATATGTGATAGTCATATTTTCAAGGTATGGATG570.12389957613302902No Hit
TCCCAAAGGCATAAATATGAGAAGAAATTATTACCCGGCATGGTGGCTCATACCTGTAATCCCAGCACTTT570.12389957613302902No Hit
CTGCTATGTTGGCTGCCCAGGATGTGGCCCAGAGGTGCAAGGAGCTGGGTATCACCGCCCTACACATCAAA560.12172589935876535No Hit
ATTCTAATCCATCTATTCAAGGTTGTAAGACTGTTTTCTATTCTAGTTGTAATGATGCCATGGAAATGACC560.12172589935876535No Hit
GTTATTTACTTTTAATTCATGTTGGAGCTCCTCCTGCCACTGCAAGAGCAGGAGCTGTGTGTCCTGGTCAC560.12172589935876535No Hit
CCTCAGTACCCATCCCAACACTGAAACTGTGTTTGAAGCCTTTCTGAAGAGCTACTCCACCTCCTCCAAAA560.12172589935876535No Hit
CATCCTGGGTCACAGACTGGGATGAGCAACGGGCTGAAGGCACGTTTCCCGGGAAGATCTGAACTGGCTGC550.11955222258450168No Hit
GTTCGAGGGGCCAAGGCAGAAGAAATCTTGGAGAAGGGTCTAAAGGTGCGGGAGTATGAGTTAAGAAAAAA550.11955222258450168No Hit
GTGCGTATTGAGCACATTAAGCACTCTAAGAGCCGAGATAGCTTCCTGAAACGTGTGAAGGAAAATGATCA530.11520486903597435No Hit
GTACAAAAGACTGACCTCTTACATCAAGGAACTTCCTTTCCCAGAGCTCCTCATGGAATCAAGCTGAAGTC530.11520486903597435No Hit
GGTAGTGACCATCGAAAGGAGCAAGAGCAAGATCACCGTGACATCCGAGGTGCCTTTCTCCAAAAGGTATT530.11520486903597435No Hit
CCTAAAGTCACATCTAGGAACTTTGAGAAAAGTATCACAAAACTTGGGAAAAAGGGTGTAAAGCAGTTCAA520.11303119226171068No Hit
ATGTATTATTTATGTACATGTACAGGACACCATTCAAAATGGCTTGAAAATATTCAGCATGTTTGTTATTT510.11085751548744702No Hit
ATTTTGGATCTATCACCTGTCATCATAACTGGCTTCTGCTTGTCATCCACACAACACCAGGACTTAAGACA490.10651016193891968No Hit
ATTTTGTGTTGTCTCACAGAAAGCCTTATCATAAATTCCATAATTCTAATTAATTTACCAAGATAATGTAA490.10651016193891968No Hit
GTAGAGAAGACAGCTGAGGATGCCAAGGACTTCTTCAAGAGGAAGATAGATTTTCTAACCAAGCAGATGGA490.10651016193891968No Hit
GTGCTGGGTAGGCCAGGTTTCAGCATCGCAGACAAGAAGCGCAGGACAGGCTGCATTGGGGCCAAACACAG490.10651016193891968No Hit
CTTGAAGGCTGGCCGGGCGTACCACAAATATAAGGCAAAGAGGAACTGCTGGCCACGAGTACGGGGTGTGG490.10651016193891968No Hit
GTAGTAGTCTTAAGAGTAAAGCTTTCCAATTTGAATTTAGATGATCACGCAAAGAAGAAATTAATTAAACT490.10651016193891968No Hit
GAATAAAGGTGCTAGAAGAACAAATAAATGAAGGAGAACAACAGCTAAAGTCTAAAAATCTGGAAGGCAGA490.10651016193891968No Hit
GTGCTGAACACCAAATCAACCTAATTAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGCCTTTGT490.10651016193891968No Hit
CTCTATAGCCAAGGAAGGCTTCGAGAAGATTAGCAAAGGTGCTAATCCAGTGGAAATCAGGAGAGGTGTGA490.10651016193891968No Hit
ATTTGAAGAATTTTGTTTTTGAAGTAATGCTTTAAGGAGTATAAGATATTCAAGATAAATTATACTATAAA480.104336485164656No Hit
GTGGTGCTAAGCGCCATCGTAAGGTGCTCCGGGATAACATCCAGGGCATTACAAAACCGGCTATTCGCCGT480.104336485164656No Hit
GACTGAAGCAGCTCAAGACCAATTTTGCCTCTTTCACAAAAATAACTCTGAGTTTGATATTCAAGTATATT480.104336485164656No Hit
GTGAAGACCTGGAGCGACTGAAGAAGATTCGGGCCCATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTC470.10216280839039234No Hit
GATGATGAAGATAAAGAAAGTGAAGAGGAGCCACCAAAAAAGACAGCCAAAAGAGAAAAACCTAAACAGAA470.10216280839039234No Hit
ATACATGTATTCAGCTTTTAATAACTGTGTTTCTGGAGGAACTTGAAACAACAGCCATCTGTATGCATGCC470.10216280839039234No Hit
CACAGAAGGGTGTTCATTTAGAAGAAAGCAACACTAATGATTCAAACAGCTTCCTGAATTTTAATTTTGTG470.10216280839039234No Hit
ATGTTAATTTCGGCAGAGCTCTGGTTAACTGTAATCCACTTAAAGATGGCTTGCAGAACTCCACCATTCTG470.10216280839039234No Hit
TTCTAGTTGTAATGATGCCATGGAAATGACCAAATGTCTCTCAAATATGAGAGAGGAGAAAAATTTGCTAA470.10216280839039234No Hit
GTATATAAGGTAGTATTCAGTGAATACTTGAGAAATGTACAAATCTTTCATCCATACCTGTGCATGAGCTG470.10216280839039234No Hit
CTGATGATAAACACAAGCTTATAACCAAAACAGAGGCAAAACAAGAATATCTTCTGAAAGACTGTGATTTA470.10216280839039234No Hit
CCTTATACCCAAAACACTTACTACCAACACAACTGTTAAACATTCTATACAAAAAAATTGTATGATCTGGT470.10216280839039234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)