FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96629.000300210147103No Hit
GAATAAAGAAGTAGTTACAGATAAAGAAAAATTTAGTAACCTACAATAACAAGGTGAGAAACGCTCAAAAA431.29090363254278No Hit
AGCTAACTCAGCCTTTGTGGAACGTCTTCGGAAACATGGCTTAGAAGTGATCTATATGATTGAGCCCATTG391.1708195737015912No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC381.140798558991294No Hit
GGTTTGGTAAGCCATCCAAGAATAGAGTGTGGCTTCTGGGTGTAGTCTCTAAAGGAGAACACAGTAATTGG280.8405884118883218No Hit
GGGCAGCAAGGCTAAGTTTGCCGGCAGGAACTTCAGAAACCCCTTGGCCAAGTAAGCTGTGGGCAGGCAAG260.7805463824677273No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC250.7505253677574302No Hit
GTTTTATGAGAATCCAGACCTATGAGGATGCTCAGATATTCTGCCCAGGATTCCCACTCTAGGGTATGCGT250.7505253677574302No Hit
ACCAAGAACTGAATTACTGTCTGCCCATTCACATCCCATTCCTGCCTTTTGCAATCATGAAACCTGGGAAT230.6904833383368358No Hit
ACATGACGAGTTGATGGGTGCAGCACACCAACATGGCACAGGTATACATATGTAACAAACTTACACGTTGT210.6304413089162414No Hit
TGAAAATGGTCACCTGCAGCTACTCAGGAGGCTGAGACAGGAGAATCGCTTGAATCTGGGAGGCGGAGGTT200.6004202942059442No Hit
TAAATAATGCATTGTTTTACTTAGTAAGAAAGAACCAATCTCTGATTCTCAAAGCTTGGGTTCTCATCAGC200.6004202942059442No Hit
GACCTGTTTGAATTTTTTCTGTAGTGCTGTATTATTTTCAATAAATCTGGGACAACAGCAAAAAAAAAAAA200.6004202942059442No Hit
GATAAAACTCTCTGTGTACTATCGTGGCAATGCTTGGAGATCCCTGTTCACCCAAGAATCTCTTTATTGGG190.570399279495647No Hit
GCTGGCTATAAGAAAAAATTATGGAAAAAGACACCTGCAAGGAAGAAGCGATTGAGGGAATTTGTATTCTG190.570399279495647No Hit
GTCTTGAAAGAGGTGATTACAATAAAATAAGGTAATTAAGGTGGGCTGTAACCTAAAATCACTGGTATGTT190.570399279495647No Hit
GAGTTCACTGTGTGTCAGAACAACTTTCTAACAAACATTTATTAATCCAGCCTCTGCCTTTCATTAAATGT180.5403782647853498No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA170.5103572500750525No Hit
CCTCATTATTATCTAGCTAAGCGGAACATGTGCTTCATCTGTGGGATGCTGAAGGAGATGAGTGGGCTTCG150.45031522065445817No Hit
GGCAGGAGAATGGCGTGAACCCGGAAGGCGGAGCTTGCAGTGAGCGGAGATCGCGCCACAGCACTCCCGCC150.45031522065445817No Hit
GCCTAAGCATTGCCTGGCCTTCCTGTCTAGTCTCTCCTGTAAGCCAAAGAAATGAACATTCCAAGGAGTTG140.4202942059441609No Hit
TGCTTATGCTTACCAAGATCAAGTGCATTGAGGGGCAGTTTTGTTTGCCTGAATAAACGTAAAGGACAAGT140.4202942059441609No Hit
CATTCTAGAAGGGGTGGCTGGGTCTTCAAGGATCTCCTGTTTTTTTCAGGCTCCTAAAGTAACATCAGCCA140.4202942059441609No Hit
CCCTCTGGGTGGCTTTGTCCACTATGGTGAAGTGACCAATGACTTTGTCATGCTGAAAGGCTGTGTGGTGG130.39027319123386367No Hit
GCAGTGCCTCTGTAGGAGGGGTGCTGGGCTCTGGCCTTCTCATGCAGAGAGGTCCGGGACAGGGTCAGTAT130.39027319123386367No Hit
GCAAACAGGCATTCTCACAGCTGGGTTTATAGTCTTTGGGCCCCTTACTCAGTATCCTGGGAACCCTGGGC130.39027319123386367No Hit
ACACACCACACACACACACCACATACACCACACACACACGTTCCCATCCACTCACCCACACACTACACAGA130.39027319123386367No Hit
GTTTAAACAATTTGTTAAAAAATTTTCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTT130.39027319123386367No Hit
GTATTAATACTACCCATAGAAAACTTGACATGTAGCTTCTTCTGAAAGAATTATTTGCCTTCTGAAATGTG130.39027319123386367No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAAT130.39027319123386367No Hit
ACCAACATATCCATTAAATCCTAATATAGAGCATCTGTTTTTAGAGTTGTGGCAACAGAGGATACAAAGGT120.3602521765235665No Hit
ATATTTTGCAACTATTATATCTTCTGCAGTTCCCCACCAACAATGTATGAGAGTGTCTGCTTTTACTAGCC120.3602521765235665No Hit
GGAGATGACATGTTTTTAGAATTGCTGTGCAAGGCTCACCCTCTCTCTGTCAACACTGGAATAAACTTTGC120.3602521765235665No Hit
GGAACACCCGGAAAGACTGAAAGAATACATTTTATATCTGGATTTTTACAAATAAAGATGGCTATTATAAT120.3602521765235665No Hit
GTGCTCTGCGTGGCGGAGAACGTGTTTGCTGTACGCTGTGCTCAGCTCACCCACCAGCTGCTGGAGCTGAG120.3602521765235665No Hit
GAATCAGTCACTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTCATTG120.3602521765235665No Hit
GGAGTGTGGAAGAGAAATTAGTGCTGAAAATAAAGAGCTCGAATCATCAGTGTATGAATAGTAGTTAAACC120.3602521765235665No Hit
CCTGTAATCCTAGCACTTTGGGAGGCCAAGGTGGGTAGATCGCTTGAGCTCAGGAGTTCAAGACGAGCCTG110.3302311618132693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.3302311618132693No Hit
GCTTGAACTCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCATGCCACTGCACTCCAGCAGCCTGGGCAACA100.3002101471029721No Hit
ACTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.3002101471029721No Hit
ATGCTGGCCTGCGCCTGCACCTGCGCCTGGCTCTGTCACCGCCATGCAACTCCATGCCTATTTACTGGAAA100.3002101471029721No Hit
AGTTAACACTGAAAGTTCTAGCTTAAGTGTTGAAACTTTTGTGGAAAAAAAAAATCACTTTTGAAACTCAG100.3002101471029721No Hit
GTATCTTATAACTTATTAAATAAAACATTTGCTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.3002101471029721No Hit
GTTTAAATAAATGTAAAAACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.2701891323926749No Hit
GAATGATAGGCTTGTCAGAGCCTATAGCCAGAACTCAGAAAAAATTCAAATGCACTTATGTTCTCATTCTA90.2701891323926749No Hit
GGTTTGGATTTTAATTGTGATGCAGAAGTTATAGTAACAAACATTTGGTTTTGTACAGACATTATTTCCAC90.2701891323926749No Hit
GGAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC90.2701891323926749No Hit
ACTTAGAATAACTAAGTTTATTAAATTCTAGAAAGATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.2701891323926749No Hit
GGACCTAAAAGACCTGTGACTAGTGAGCTCTAGGCTGTAGAAATTTAAAAACTACAATGTGATTAACTCGA90.2701891323926749No Hit
CTTTGGTTTCTATGATACATTCTACTGGTTCTCCCAACACCTCTCCAACCATTTCATCATTTCCTTTATGG90.2701891323926749No Hit
GCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAA90.2701891323926749No Hit
GTTTTAGTTTTGATAATAAATTCTTTGGAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.2701891323926749No Hit
CTTTCTTTTGGAATGTATTTGATTGATAAGAAAGTTTAAACATTGTTTTCACCTCAATGTAGAAATACAGT80.24016811768237767No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT80.24016811768237767No Hit
CTGAGAGTTGATTGTGCCAGGCGCCAGTTTTTCTTGTTATGACTGTGTATTTTTGTTGATCTATACCCTGT80.24016811768237767No Hit
CCGCAAAGTGGGTCTCATTGCTGCCCGCCGGACTGGACGTCTCCGGAGAACCAAGACTGTGCAGGAGAAAG70.21014710297208045No Hit
CACCTATTGATGGGAATGTGTGCGGGCTCAGCCTTCCTGGACACACTAGGTGTGGGAAGCCATAGGAGCCT70.21014710297208045No Hit
CCACGCGTGGAGGAGAATCCGGCCTCCAGACACAATCTCCAGGGCCCACTGGATGGGCCTCCGCTCCTTCT70.21014710297208045No Hit
GCTTGAACCTGGGAGGCAGAGGTTGCGGTGAGCCAAGATCATGCCATTGCACTCCAGCCTAGGTGACAGGG70.21014710297208045No Hit
GGGAATGAAGAGCGTCCTGGGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.21014710297208045No Hit
GTCTCTGCACAGAAACTTGAACTCCAGGATGGAATTCTTCCTCCTCTGCTGGGACTCCTTTGCATGGCAGG70.21014710297208045No Hit
GTGTACTGGTTCTGTGAACCACCTGAAAAAACAAATTAAATGTATTAAACCATAAAAAAAAAAAAAAAAAA60.18012608826178325No Hit
AAACAAAACAGTATCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.18012608826178325No Hit
GGAATTGGCAGAGGAAGTAGGTGTCTGATAACCCTTTGTGGAGAATGAGATTCCCCCCACCTGTGTGAGAA60.18012608826178325No Hit
GGAGTCTGGGATGCAGAACATGAGTATTCATACGAAGACCACCAGCGGCTATGCAGGTGGTCTGAGCTCGG60.18012608826178325No Hit
CACCAATATAGATTACTTCATACCACATTGTCAAGGAAAGGACTAGAAGAATTTTTTGATGACCCAAAAAA60.18012608826178325No Hit
ATGAGGTCCTAGCCCCTGGCTGCCTGGATGCGTTCCCCCTGCTCTCAGCATATGTGGGGCGCCTCAGTGCC60.18012608826178325No Hit
ATGGTCAACGTACCTAAAACCCGAAGAACCTTCTGTAAGAAGTGTGGCAAGCATCAGCCTCACAAAGTGAC60.18012608826178325No Hit
GAGAAAGATCCACCTATGACCTCAGGTCCTCAGAACAACCAGCCCAAGGAACATCTCACCAATTTTAAATC60.18012608826178325No Hit
CTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.18012608826178325No Hit
CCAAAGTTCTGAGGCATTAAGCCAGCAGAAGCAGGGTACCCTTTGGGGAGCAGGAGGCCAATAAAAAGTTC60.18012608826178325No Hit
ATAATAAGTTATTCACCTGAGTATGCAATAAAGATGTGGTGGCCACTCTTTAAAAAAAAAAAAAAAAAAAA60.18012608826178325No Hit
CCAATAAACATTAAAACAAAGACTGTATGTGAACTATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.18012608826178325No Hit
GGGGAGCCTCGGCCCTGAACAGAGCAGCTGCTGATGTCTGGAGGCTGATTTTCCTGTTCTCTGTTCTCCAC50.15010507355148606No Hit
GGATCACTTGAAGCCAGGAGTCCAAGATCAGCCAGGACAACACAGCCAGACCCCATCTCTACTTTAAAAGA50.15010507355148606No Hit
ATGCTGGACCGAGAGCCCTAGCTTCTGGATCCAGGTCTAGGCCCTTCATCTGCTGCTCTGTGGCCCAGGGC50.15010507355148606No Hit
GCTTGTTTTGTTTCATCCTGTCCGTAAGGGGTCAGCGCTCTTGCTTTGCTCTTTTCAATGAATAGCACTTA50.15010507355148606No Hit
CTACTACACCCTGCATGCTTCGAAACTCTGACAGCATACTGGAGGCCATTCAGAAAAAGCTTGGAATAAAG50.15010507355148606No Hit
TAATTAAAAGTATTTAATTTGAAGCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.15010507355148606No Hit
AGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAATTGAAAACAAAA50.15010507355148606No Hit
CCACAATGACATTTAAGCACAGGATCATTATTAGTCTATGTTTTTAATAAACATATCAATTAAGAAAAAAA50.15010507355148606No Hit
GGCGACATGCGAGGGAGAACAGCATCTCAGTGGTGATTTCCAAACCAAGCCTTTGTTTTCGGTGTGGGGTT50.15010507355148606No Hit
TCCTACTTTTGAGTTTCTCTGTGGAAATAAAACATGAATCTTGTTTCCCTAAAAAAAAAAAAAAAAAAAAA50.15010507355148606No Hit
TCATGCTGGATGCTGCCTTAGCGCTGGCGGCCGAGATTTCCAGCAAGAGCCCCGTGGCGGTGCAGAGCACC40.12008405884118883No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTT40.12008405884118883No Hit
CCTTGTAACAATAAAATCTATTTAAACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12008405884118883No Hit
AGTCTGAGGGCCTCACCAAGGAGTACGACCGCTTGCTGGAGGAGCACGCAAAGCTGCAGGCTGCAGTAGAT40.12008405884118883No Hit
CAGTGAGCACATCTAGCTGATGATAAATACACCTTTTTTTCCCTCTTCCCCCTAAAAATGGTAAATCTGAT40.12008405884118883No Hit
CACGGCCGAGTTTGAGGAGGCTCAGACCTCGGCATGTCTGTTACAAGAAGAATTGGAGAAGCTCCGCACAG40.12008405884118883No Hit
CTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTC40.12008405884118883No Hit
TGATGACATTGACCTGTTTGGCAGTGACAATGAGGAGGAGGACAAGGAGGCGGCACAGCTGCGGGAGGAGC40.12008405884118883No Hit
TCTTTGTCCTCTGTACTGTCTCTCTCCTTGCCCCTAACCCAAAAAGCTTCATTTTTCTGTGTAGGCTGCAC40.12008405884118883No Hit
GGTCACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12008405884118883No Hit
GGCAGAAACCGTCTGGAGAATGATGGCGCCACTGCCTTGGCAGAAGCTTTTAGGGTCATCGGGACCCTGGA40.12008405884118883No Hit
GTAACATGTGTTGGCTTCGTGATTAAAATGCTCCTAATCAAGATTGTTTGAACACATGTATTTATAAAACA40.12008405884118883No Hit
ACATGGTACACACACCCCCACCTCCTGCAATTAAACAGTAGCATCGCCAAAAAAAAAAAAAAAAAAAAAAA40.12008405884118883No Hit
GTTCTGCCCCTGCTGCAGCAGAGGAGAAGAAAGATGAGAAGAAGGAGGAGTCTGAAGAGTCAGATGATGAC40.12008405884118883No Hit
GTTTCAGAGCATCTGATTTGTCAGACCCTCACCAAAAATGGGCTTTTATCAAAATGTAATAAAAAATAAAT40.12008405884118883No Hit
ACAATTTTTTCTATGAACCTTTACTTACTTGACTGGATTGGACTAAAAGCACTGATCAGAGGCCACGACAT40.12008405884118883No Hit
ACACACCACACACACACACCACATACACCACACACACACGTTCCCATCCACTCACCCACACACTACACAGC40.12008405884118883No Hit
TCTTAGAAGAGTACTTGAGCTCCACTCCGCAGCGTCTGAAGTTGCTGGACGCGTACCTGCTGTATATACTG40.12008405884118883No Hit
GATAAATTCTGTACCTATACTTGGTTCTATAAGTCCCACATGTTGCCTATTGTCAGAAACAAACTGTTCCT40.12008405884118883No Hit
TAATGAATTAGTCTAATCATGTTTTCTGAAAATATAACCAGCCATTGGCTATTTAAAACTTGTAATTTTTT40.12008405884118883No Hit
ATGCTGGATCATGTGCTACTGGTATAAAGTTCTGGCCTTCTACCTTAAAAAAAAAAAAAAAAAAAAAAAAA40.12008405884118883No Hit
ATAAAGATGTGAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12008405884118883No Hit
GTCTGAATCCAGTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAAAAAAAAAAAA40.12008405884118883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)