FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3634
Sequences flagged as poor quality0
Sequence length71
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108629.884424876169508No Hit
GTTGGTCAACTCTGATACAAAGTATATATACAGTTCAGTATTGTCTCTGTTCATTTTGTTTTTATTTCATT401.1007154650522841No Hit
GATTCTTGGAGCTGAAAAGGCACTTTTCAGAGCCCTCAAATCTAGACGGGATACCCCTAAGTATGGTCTCA360.9906439185470555No Hit
CCCCCAGTTATATATTCTCATTTCACTGAAGACCTTTGGCCAGAGCATAGCATAAAAGATTCTTTTCAAAA330.9080902586681342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280.7705008255365988No Hit
GGGCCTTATATGACCACCTAATGGATTTCCTTGCCGACCGAGGGGTGGACAACACTTTTGCAGATGAGCTG280.7705008255365988No Hit
GGTCCATGGAACAATCACAGGTGTGGATGTCAGCATGAATACACATCTTAAAGCTGTGAAAATGACCCTGA260.7154650522839846No Hit
GGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTCTTAACTA250.6879471656576774No Hit
GGAAAACTTACCTATTGAAGCTGTTTATCACAGGTTAGACTGAAAAGTAGAAATTAACAGTTACACCTGTT240.6604292790313704No Hit
CTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACAGCAGCAACTCCATGC240.6604292790313704No Hit
GGCCAATATGCTGAAGCCCCATCTCTATGAAAAATACAAAAATTAGCCAGGTAGGGTGGCAGGTGCCTGTA230.6329113924050633No Hit
GGCTATTCCATGTATCGGGAATTCTGGGCAAAACCTAAGCCTTAGAAGAAGAGATGCTGTCTTGGTCTTGT230.6329113924050633No Hit
GATTACGGGTGTGAGCCACCACATCTGGTCTTAAACTAACAAATATTAAATACTGTATTTTTATCTGTATC220.6053935057787562No Hit
GCTACTCAGGAGGCTGAGGCAGGAGAATGGCGCGAACCCGGGAGGCAGAGCTTGCAGTGAGCCAAGATCGT220.6053935057787562No Hit
GTGTTCACACCTTCTTTGATCACCAGGATCAGGTCTGGGGAGTAAAATACAATGGAAATGGTTCAAAAATT210.5778756191524491No Hit
CATACTAAGACCTGTTGAGCAAAAGTCCTAACTGTTCGACTTGGATCTAATCATGAGGAAATAATCAAACA210.5778756191524491No Hit
GTGTGGACACTTTTGACAGCTTGGCAGATTTTAACTCCAGAAGCACTTTATGAAATGGTACACTGACTAAT210.5778756191524491No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT200.5503577325261421No Hit
AGCCAGGGCTTACCTGTACACTGACTTGAGACCAGTTGAATAAAAGTGCACACCTTAAAAAAAAAAAAAAA190.5228398458998349No Hit
GAAAAAATGATTTAATCTGTAATAAACTGGTTTATTGTGCAGTGACTGTAATATACTAGAGTTATAATAAA190.5228398458998349No Hit
GATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAGAAAAAAAAA170.4678040726472207No Hit
GGGCCTGGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATGCGACGAACCTCTGAACATCCTGGTGA170.4678040726472207No Hit
GGTTCCTCCCACCCCTGCTGAGATCCCTAGAGCTATTCAGAGCCTGAAAAAAATAGTCAATAGTGCTCAGA170.4678040726472207No Hit
GCTGTTGGTGTTAAGAAGCAGAAGAAGCCTCTGGTGGGAAAAAAGGCTGCAGCTACCAAGAAACCAGCCCC170.4678040726472207No Hit
GTCTCCTTATATGCTACACACAACTCTTGGAAATGAAGCATTCAAAGACATTCCAGTGCAAAGGCATGTGA170.4678040726472207No Hit
GATGATGAAAAAGAGGCTGAGGAAGGAGAGGATGACAGAGACAGCGCCAATGGCGAGGATGACTCTTAAGC170.4678040726472207No Hit
GGCTTCCAAGAAGGAGATCAAAGACATCCTCATCCAGTATGACCGGACCCTGCTGGTAGCTGACCCTCGTC170.4678040726472207No Hit
GTCTATAGTTTTGGCCTTAACTATGCCAATCTAATTATAATTCCCTGTATTTAAAATGGTTTCTTTTACTT160.4402861860209136No Hit
GCCCCACAGCCCCTGACACTCTAGGTTGTTACCCCTTCTACAAAACTGACCCGTTCATCTTCCCAGAGTGC160.4402861860209136No Hit
GGAGAAGAAACTAAGTGTGATGGAGAGCTCAAAATCACACGCCGCAGAGCCAGTCAGATCACACCCGCCAG150.41276829939460646No Hit
TGATTCATGAGCCAGAACCACATATTCTTCTCTTTAGACGACCTCTTCCAAAAGATCAACAAAAATGAAGT150.41276829939460646No Hit
CCTTTCCTCTATCATAAGTCGCCAAAAACAAATGGGGCAGGAAATTGGGAATGAATTGGATGAACAAAATG140.3852504127682994No Hit
AAAGTACACAAACCTGGCTACGGTGATGAGTTAGCTTCTGCTTACTACCTGTGACAACCCAAGTGGGTGAC140.3852504127682994No Hit
GTACAAAGACAGCACAAGAAAAAAACAGATTTCATAAAAATAGTGATTCTGGTTCTTCAAAGACATTTCCA140.3852504127682994No Hit
AAGCAGCTACACACATTCACCTAATTCCATCTGAGGGCAGAACAACGTGGCAAGTCTTGGGGGTAGCAGCT140.3852504127682994No Hit
CCTGTGGCCCTGGCTGCTTTACACAATCTGTTCTATAAGGTTCAGGTGTTTTCAAGTTGGAAAGATCATAA140.3852504127682994No Hit
GCTAAAACATGATGATTAAGTGCACACCGTGTGCCATAGAATGGCACATGTCATTGCCCACTTCTGTGTAG140.3852504127682994No Hit
ACACTGTTTTATGAGTTATATAAAATGTTATAAAAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3577325261419923No Hit
CCTTCAGCAACATATATCCTCGACCAGATGCAGTGCTATAAGAACAGAACGCATTTTGGATGTTATTATTA130.3577325261419923No Hit
GGGTTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3577325261419923No Hit
CTGACATTCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3577325261419923No Hit
GGGTTCACGGAAAAGCCAGAACCTGCTGTTTTCAGGGTGGGTGATGTAAATATAGTGTGTACATAATAAAG120.3302146395156852No Hit
GAGCTAAAAGTAGCTCCCAAGAACCTGCTTAACACAATCGCCTGCCCCGGCCTCAGAAAGCACTTCCATTG120.3302146395156852No Hit
GGTACCTGGGTTCAACTAAAGCGCCAGCCTGCTCCACCCAGAGAAGCACACTTTGTGAGAACCAATGGGAA120.3302146395156852No Hit
GTTCTTTCTTACACAAGAGCTGGAGCCCGTGCCCTACCACACATCTGTGGAGATGCCCAGGATTTGACTCG110.3026967528893781No Hit
GTTAACATTATATTTGGATCCAATTTTCCTGAGATATTGAAATAGTGAAATCACCAAATAAATGATATGAA110.3026967528893781No Hit
GCGTCAGAGAAGTGCAGCTGCTGCGACTGATGCCAGGACAACCTTTCTCCCAGATGTAAACAGAGAGACAT110.3026967528893781No Hit
GTCAGTCACCGCCTCTGCGCTATTCCTCAGGTGTAATATTGTTAAGAAAGAAAGGGGCAAGGAAAGTAAGA110.3026967528893781No Hit
GAGATGCTAAAAATCAAGCAAGAACAGTTTATGAAGAAGATTGTTGCAAACCCAGAGGACACCAGATCCCT110.3026967528893781No Hit
ACCATGCGGGCCGTGCGCAGGGAGAAGGACCAGTTCAAGATGCCCCGGGACTGAGGCCGCCCTGCACCTTC100.27517886626307103No Hit
AGTTTGACAGTTTATGGAGGCTTTTGAATCGTAATAGCAATGTGAGGGTGAGGTACACCTACAGACATTAA100.27517886626307103No Hit
CCCTTTATGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.27517886626307103No Hit
AGTTAGTATCCACATCATAAATTCAGAGTGATGTTTAGCAGTAAATCAATATTTTGAAGTGATACACAGAT100.27517886626307103No Hit
GCATATGAGCCTGAAATATCCAGGCTCCATGACAGTCTTGCCATAGAAAGAAAAATAAAGTAGCCAATTCC100.27517886626307103No Hit
CTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.27517886626307103No Hit
GCTTTATTTTGGCAGATACTCAGTACATTCTTTTCTCTACATCCTCATCAACATCTGTTACTTTTTGACTT90.24766097963676387No Hit
CCGCAGCCCCTGCTGCCCAATAAAACCACTCCGACCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.24766097963676387No Hit
GCTTTCTCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.24766097963676387No Hit
TCTTGACTCACTGCAACCTCTGCCTCCCGGCTCAAGCCATCCTCCCACCTCAGCTAGAACTACAGGCGTGA90.24766097963676387No Hit
ATTTTGCCATTTATCTACAGAAATTTGATTATAAATATGTTCCTTTTTCAAAGAAACTTTATTCTAGAACA90.24766097963676387No Hit
CATGTGTCAATGTTTCTAGTATTTCAAAGAAGCAATTATGTAAAGTTGTTCAATGTGACATAATAGTATTA90.24766097963676387No Hit
GGTGAAGTGACCAATGACTTTGTCATGCTGAAAGGCTGTGTGGTGGGAACCAAGAAGCGGGTGCTCACCCT90.24766097963676387No Hit
CCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAA80.2201430930104568No Hit
AGTCCTAGAAAATAGAAATGTAATTTTAAACTATTCCAATAAAGCTGGAGGAGGAAGGGGAGTTTGACTAA80.2201430930104568No Hit
GAACCAATAAGCCATGTGGGAAGTGCTACTTTTACCTTCTGATTAGTAAGACATTTGTGGAAGCAGGAAAA80.2201430930104568No Hit
CCAGACACACTGATTGGAACTGTATTATATTAAAATACTAAAAATCCAAAAAAAAAAAAAAAAAAAAAAAA80.2201430930104568No Hit
GAATCAAGAATCAATGCGTGGAATTCTCCTACACTTCCTATTTATGAGCCAGGACTAAAAAAAAAAAAAAA80.2201430930104568No Hit
ATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAAT80.2201430930104568No Hit
CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.2201430930104568No Hit
CAGGAGAATCACTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCAAGATCATGCTATTGCATTGCAGCCTG80.2201430930104568No Hit
GTTTTATAATAAAATTCTTGAGAACCTTCCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.2201430930104568No Hit
AGAAAACCTTACGTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1926252063841497No Hit
GTTCTTGAATAAAACTTGGGAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1926252063841497No Hit
GAGCCCTATCTCCTCCTGGTTTTGTTGCTGACATTGCAGCTGATCATGCCTTGACTCCTTCATCTCTAACA70.1926252063841497No Hit
ATAAAAAGTAAACATGACACCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1926252063841497No Hit
GTAATAAATCTTTGTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1926252063841497No Hit
GGTACTTAGAGTTTCTGTTTGATTCTTTTTTAATAAACTACTCTTTGATTTAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
CAGGGCAGGCAGCTGTGCATGTTCTCTCAACTAAAGGTCTTGTGAGAGGAAAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
GGGTGATCCCTGCCATCTCTGCAGTGGCCGTCGCCTTGATGTATCGCCTATACATGGCAGAGGACTGAACC60.1651073197578426No Hit
TCTTGACTCACTGCAACCTCTGCCTCCCGGCTCAAGCCATCCTCCCACCTCAGCTAGAACTACAGGCGTGG60.1651073197578426No Hit
GGGATAGATGCTTGTTTGTAAAAACTCACCTTAATAAAGACTTAGATGTTAAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
TCCCTACACGACGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
CGGCGCGCTGCCCGAGGGGAGCAAGGACAGCTTTGCAGTTCTCCTGGAGTTCGCTGAGGAGCAGCTGCGAG60.1651073197578426No Hit
CAATAAAGTTTGTGTTTATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
ACTCAGACCATAAAGCACTCCTGTTTCACTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
GGGTAGCTGAGTCTGCTTAGGGGACTGCATGGGAAGCACGGAATATAGGGTTAGATGTGTGTTATCTGTAA60.1651073197578426No Hit
GCTCCAGGGCTACCTGCGATCTGTGTTTGCTCTGACGAATGGAATTTATCCTCACAAATTGGTGTTCTAAA60.1651073197578426No Hit
GACCAAAAATTTGTCACAGAATTTTGAGACCCATTAAAAAAGTTAAATGAGAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
GGCCAAGATCCAGGATAAGGAAGGAATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGCAAGCAGCTGG60.1651073197578426No Hit
GGTTTGGAAAAATTTTGCTGTTATTATAGTAAACATACAAAGGATGTCATCAAAAAAAAAAAAAAAAAAAA60.1651073197578426No Hit
GAATAAAATCCCCGCAGCCTACTGCACTTCTTGGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13758943313153552No Hit
GTTAATAAATAGTTTATATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13758943313153552No Hit
GGAACAAATTGCGAAGAGACGCAGACTTTCCTCTCTGCGAGCTTCTACTTCTAAGTCTGAATCCAGTCAGA50.13758943313153552No Hit
GTATAGCTGGAATTAAGTGTTGTCTTGGAGCTGTTGTACATTTAAGAATAAACTTTTGTAAAAAAAGAAAA50.13758943313153552No Hit
GTCTTTTACAGTTCGTTTTCTACAGGAACTGTAAAAGACCATGCAAGAGGCAAAATAAAACTTGAAGTGAA50.13758943313153552No Hit
TTGTTACATGGACATATGTAGCAACACAAGTCGGAATAGAATGGAACCTGTCCCCTGTTGGCAGAGTTACC50.13758943313153552No Hit
GTATGAAGGCTTTTGGTCTCCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAGAC50.13758943313153552No Hit
GACCACTCACCCAACTCCTGAGATCTTATCAGGAAGCTGCTGATCACCAACCTCAGATTTGGCATCCTTTC40.1100715465052284No Hit
GGTGGCAGTTCAGAAATAAAGGGCCCATTTGTGGGATGCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1100715465052284No Hit
GGTACCTGAGAGCCCCTCAGGGGAGGAGGCCGAGTGTCTGGGCTGTTACAGTATTTGCTAAATACATATTT40.1100715465052284No Hit
CTGAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1100715465052284No Hit
GTTCCACGACTCCAAGATCAAGTTCCCGCTGCCCCACCGGGTCCTGCGCCGTCAGCACAAGCCACGCTTCA40.1100715465052284No Hit
GATCACGCCACTGCACTCTAGCCTGGGCGACAGAGAGAGAGACTGTCTAAAAAAAAAAAAAAAAAAAAAAA40.1100715465052284No Hit
GCACAAGCCACGCTTCACCACCAAGAGGCCCAACACCTTCTTCTAGGTGCAGGGCCCTCGTCCGGGTGTGC40.1100715465052284No Hit
TGTCAATATAGTCAGCCGATCGATCGACCTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1100715465052284No Hit
GCCTATACTTTCTACCAGAAGCTTGAAGGGTTTGGTTAAGAATATTCCCCAAAGCACTGAGATACTAAAGA40.1100715465052284No Hit
ACTCCCGGGTGGGGAGGTGGCCAAGGATGCTGGAGCTGAATCAGACGCTGACAGTTCTTCAGGCATTTCTA40.1100715465052284No Hit
ACACGACGCGGTACTGATCATTCTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1100715465052284No Hit
GATGATGAAAAAGAGGCTGAGGAAGGAGAGGATGACAGAGACAGCGCCAATGGCGAGGATGCCTCTTAAGC40.1100715465052284No Hit
GTCCTCGGCCACATTGTGAACTTTGGGGGATGCTCGCTCCAACCGACTGCTGTCACCTTCACCGTTCCAGT40.1100715465052284No Hit
GTCTCCTTATATGCTACACACAACTCTTGGAAATGAAGCATTCAAAGACATTCCAGTGCAAAGGCATGTGC40.1100715465052284No Hit
GGATTTGCCTCCTCCACGCTCCCTACACACCTGAGATGTAAACTGGCAGTCAGTGTTCACTCAGGACCTAG40.1100715465052284No Hit
TCTTATGTGGGTATTACAGGAATCCTTCTACAGGAAACAAAGCACATTTTCAAAATTATCACCAAAGAAGA40.1100715465052284No Hit
TCTTATGTGGGTATTACAGGAATCCTTCTACAGGAAACAAAGCACATTTTCAAAATTATCACCAAAGAAGC40.1100715465052284No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1100715465052284No Hit
GAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAAAAAAAAAA40.1100715465052284No Hit
CCTCCCAGTGGCACCCACGCCACCTGCTTTGAGGGGTTGGATCTTCCTGCTACCCTCTTGGATTCTAAGTG40.1100715465052284No Hit
CATGTGTCAATGTTTCTAGTATTTCAAAGAAGCAATTATGTAAAGTTGTTCAATGTGACATAATAGTATTT40.1100715465052284No Hit
GGTCCATGGAACAATCACAGGTGTGGATGTCAGCATGAATACACATCTTAAAGCTGTGAAAATGCCCCTGA40.1100715465052284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)