FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002879900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002879900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18745
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3962.112563350226727No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT840.44811949853294214No Hit
GATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTA800.4267804747932782No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC680.3627634035742865No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC590.3147506001600427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540.2880768204854628No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGC360.1920512136569752No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGG310.1653774339823953No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTA310.1653774339823953No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAG310.1653774339823953No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC280.14937316617764737No Hit
GTCTAGGGAACTTATAGAAATGCTGGCAATTTCATGAAACCAAAAGTTGTTACAGGCTGGAGAGGAAAACC280.14937316617764737No Hit
CCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA280.14937316617764737No Hit
GCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGAC280.14937316617764737No Hit
CATTTAATGAGGATACACTGTTCATTTTTAAAATTCCTTCACACTGTAATTTAATGTGTTTTATATTCTTT270.1440384102427314No Hit
CCTTTAGGTTAAGTTTAAGCTTTTTAAAAGTTAGTTTTGAGAATTGAGACACAATACTAATACTGTAGGAA260.13870365430781542No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG260.13870365430781542No Hit
CCATTATGCAGAATCCACGCCAGTACAAGATCCCAGACTGGTTCTTGAACAGACAGAAGGATGTAAAGGAT250.13336889837289945No Hit
ACATGATGCTGCCCATTCCACTGAAGTTCTGAAATCTTTCGTCATGTAAATAATTTCCATATTTCTCTTTT250.13336889837289945No Hit
ATGGTACACATTCCATTTGGATCACAAAAGCAAAGAATGCTGTTGTTGCTGCTGTTGCTGCTGTTTCAGTT250.13336889837289945No Hit
ATTCTAGTTTATATTTTCATTTAAAATCCTTTAGGTTAAGTTTAAGCTTTTTAAAAGTTAGTTTTGAGAAT250.13336889837289945No Hit
CTCCAAAGCCACATGTAAAGACTTGGTTATGTTTATCTAATGTTGGGTCCAAGAAGGAATTTCTTTCCATC250.13336889837289945No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA240.12803414243798347No Hit
TTACTTAAGTTTATTGATACATGCTCTTGCCACAAATTCCTCCAGTGAATTATTTCGGCTTGCAGCCCACC240.12803414243798347No Hit
CCCCATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCTGCAGTTCCCTTTGGGTTCCATGTTTTCCT240.12803414243798347No Hit
CTACAGCATGGTGGCCTACTGGAGACAGGCTGGACTCAGCTACATCCGATACTCCCAGATCTGTGCAAAAG230.1226993865030675No Hit
GTCTGTATTATTATTCACCTATAATTAGTCATTATGATGCTTTAAAGCTGTACTTGCATTTCAAAGCTTAT230.1226993865030675No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT230.1226993865030675No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTC230.1226993865030675No Hit
TATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACT230.1226993865030675No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT230.1226993865030675No Hit
ATTATGAAGTGGCTCCTCCTGAGTACCATCGGAAAGCTGTGTGAGAGGCACACTCACTCACTTATGTTTGG230.1226993865030675No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAA220.1173646305681515No Hit
ATAAAGGAGGATTTGAACAGAAAATGAGTAGGCGAGAAGCTGGTCTTATTTTAGGTGTAAGCCCATCTGCT220.1173646305681515No Hit
TAAGAAAGGTGATATTGTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACC220.1173646305681515No Hit
ACACGACGCGGGGCAGCTGGAAGCATTTGTTTTTGAAGTTGTACATAGTAATAATATGTCATTGTACATGT220.1173646305681515No Hit
AAGAAAAGGGGAGCACAAGGCCTTAATGGACATTGACTTGTGAAAACGCAAACATGAATATGGTTGGAGAG220.1173646305681515No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCAC220.1173646305681515No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1173646305681515No Hit
TAATATTACTCTGCTACAAAGTGTCTCCAACTAGAAATGATCAATGAAGTGAGAAATTGTTGAGAAGGATA220.1173646305681515No Hit
GAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAAC220.1173646305681515No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA220.1173646305681515No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCT210.11202987463323553No Hit
AAATAGCACTGAAAAAATGAGGAAATTATTGGTAACCAATTTATTTTAAAAGCCCATCAATTTAATTTCTG210.11202987463323553No Hit
GCTGGAGATATTTCGGATGATAATCTACCTCACTTTCCCTGTGGCTATGTTCTGGGTTTCCAATCAGGCCG210.11202987463323553No Hit
GTTATATATGGGGATGGTAGTTTTTTCATCTTCAGTTGTCTCTGATGCAGCTTATACGAAATAATTGTTGT210.11202987463323553No Hit
CTGGAGGAACTCCTGCCATTACCAGCTCCCTTCTTGCAGAAGGGAGGGGGAAACATACATTTATTCATGCC210.11202987463323553No Hit
TAATGTTACTGATCATGCATTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATTTATTAAGA210.11202987463323553No Hit
CATCCTGGCCTGTCCATTGGTGATGTTGCGAAGAAACTGGGAGAGATGTGGAATAACACTGCTGCAGATGA210.11202987463323553No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAA210.11202987463323553No Hit
GATGCAGAGATTGTGGATACAGAATAATGTACAAGAAAAGGACTAAAAGATTGGTCGTTTTTGATGCTCGA200.10669511869831955No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA200.10669511869831955No Hit
GCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGT200.10669511869831955No Hit
GCTCATAAGAGAGTCATGATTTTGAATCACCCAGATAAAGGTGGATCTCCTTACGTAGCAGCCAAAATAAA200.10669511869831955No Hit
AACATGGAATGCCAGACGCTGGGGATGCTGGTACAAGTTGTGGGACTGCATGCTACTGTCTAGAGCTTGTC200.10669511869831955No Hit
GCATTTATCCGAGGAAACAATGTGTTGTACATCAGTACACAGAAGAGACGGATGTGAAGACACCAAGAGAG200.10669511869831955No Hit
GTTGTCCCAAAGGGTCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAAGATTTCATGAGCAGATCA200.10669511869831955No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAA200.10669511869831955No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTAT200.10669511869831955No Hit
AATATGTATTTTGTCAAATTTGTTACTTAAATATATAGAGACCAGTTTTCTCTGGAAGTTTGTTTAAATGA190.10136036276340357No Hit
AAATTATAGCTTTCAAAAATTTAATAGAAAACAAAGAAGTTCTATTTTATTTAATTGCATTCACAAATCAG190.10136036276340357No Hit
GTTTAACCAAAACATCAGATTGTGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCGAGAAAGCTCA190.10136036276340357No Hit
AGGTTAATGTGTTCAGAAGTCGCTGTCCTTACTACTTTTGCGGAAGTATGGAAGTCACAACTACACAGAGA190.10136036276340357No Hit
GCATTGGAAAAGACATAGAAAAGGCTTGCCAATCTATTTATCCTCTCCATGATGTCTTCGTTAGAAAAGTA190.10136036276340357No Hit
GGTTATTATGATTAGCTTTGTCACTGTTTCACTACTCAGCATGGAAACAAGATGAAATTCCATTTGTAGGT190.10136036276340357No Hit
TAGTAACACTGATTTTAAAATTAAATTTCAAAAGTCAATCTCTAAGAGTAATTTATTTTTGTTTTACCAAC190.10136036276340357No Hit
CTGTGATACCTGGCACAGTGCTTTGATCTTACGATGCCCTCTGTACTGACCTGAAGGAGACCTAAGAGTCC190.10136036276340357No Hit
TCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA190.10136036276340357No Hit
CTTTTGAGCTGATTCTCAGCCCTCGGTCAAAAGAATCTGTTCCAGAATTCCCCCTTTCCCCTCCAAAGAAG190.10136036276340357No Hit
TCACTGAGGAAGAGAAGAATTTCAAAGCCTTCGCTAGTCTCCGTATGGCCCGTGCCAACGCCCGGCTCTTC190.10136036276340357No Hit
GCTCCTAGGCTGAACTCAGCAGATCGGCCCATGAAAACTTCTGTATTGAGACAAAGGAAGGGATCTGTCAG190.10136036276340357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)